tufA gene as molecular marker for freshwater Chlorophyceae

Vieira, Helena Henriques,Bagatini, Inessa Lacativa,Guinart, Carla Marques,Vieira, Armando Augusto Henriques The Korean Society of Phycology 2016 ALGAE Vol.31 No.2

Green microalgae from the class Chlorophyceae represent a major biodiversity component of eukaryotic algae in continental water. Identification and classification of this group through morphology is a hard task, since it may present cryptic species and phenotypic plasticity. Despite the increasing use of molecular methods for identification of microorganisms, no single standard barcode marker is yet established for this important group of green microalgae. Some available studies present results with a limited number of chlorophycean genera or using markers that require many different primers for different groups within the class. Thus, we aimed to find a single marker easily amplified and with wide coverage within Chlorophyceae using only one pair of primers. Here, we tested the universality of primers for different genes (tufA, ITS, rbcL, and UCP4) in 22 strains, comprising 18 different species from different orders of Chlorophyceae. The ITS primers sequenced only 3 strains and the UCP primer failed to amplify any strain. We tested two pairs of primers for rbcL and the best pair provided sequences for 10 strains whereas the second one provided sequences for only 7 strains. The pair of primers for the tufA gene presented good results for Chlorophyceae, successfully sequencing 21 strains and recovering the expected phylogeny relationships within the class. Thus, the tufA marker stands out as a good choice to be used as molecular marker for the class.

tufA gene as molecular marker for freshwater Chlorophyceae

Helena Henriques Vieira,Inessa Lacativa Bagatini,Carla Marques Guinart,Armando Augusto Henriques Vieira 한국조류학회I 2016 ALGAE Vol.31 No.2

Green microalgae from the class Chlorophyceae represent a major biodiversity component of eukaryotic algae in continental water. Identification and classification of this group through morphology is a hard task, since it may present cryptic species and phenotypic plasticity. Despite the increasing use of molecular methods for identification of microorganisms, no single standard barcode marker is yet established for this important group of green microalgae. Some available studies present results with a limited number of chlorophycean genera or using markers that require many different primers for different groups within the class. Thus, we aimed to find a single marker easily amplified and with wide coverage within Chlorophyceae using only one pair of primers. Here, we tested the universality of primers for different genes (tufA, ITS, rbcL, and UCP4) in 22 strains, comprising 18 different species from different orders of Chlorophyceae. The ITS primers sequenced only 3 strains and the UCP primer failed to amplify any strain. We tested two pairs of primers for rbcL and the best pair provided sequences for 10 strains whereas the second one provided sequences for only 7 strains. The pair of primers for the tufA gene presented good results for Chlorophyceae, successfully sequencing 21 strains and recovering the expected phylogeny relationships within the class. Thus, the tufA marker stands out as a good choice to be used as molecular marker for the class.

Correlation between short-term blood pressure variability parameters with mobil-O-graph pulse wave velocity

Marco Antonio Vieira Silva,Luiz Antonio Pertilli Rodrigues Resende,Mateus Marchiori Vieira,Camila Blanco Ferreira Jajah,Lucas Alves Berzotti,Nicole Cristine Rambourg,Ian Dias de Souza Pierson,João Luc 대한고혈압학회 2022 Clinical Hypertension Vol.28 No.-

Blood pressure variability (BPV) and arterial stiffness show an association with increased cardiovascular events. Evidences demonstrated an association between higher short-term systolic BPV and stiffer arteries. There is no previous study assessed the correlation between BPV and arterial stiffness measured by a Mobil-O-Graph device. We issued to evaluate the correlation between short-term BPV parameters and Mobil-O-Graph pulse wave velocity (PWV) among suspected hypertensive individuals under treatment.Mobil-O-Graph device estimated arterial stiffness (oscillometric PWV [oPWV]) in 649 individuals, and they recorded 24-h ambulatory BP; 428 had suspected hypertension and 221 under treatment. We analyzed the correlation between oPWV and measures of BPV: SD of 24 h BP (24-h SD), SD of daytime BP (daytime-SD), and SD of nighttime BP (nighttime-SD), weighted SD of 24-h BP (wSD), coefficient of variation of 24-h BP (CV 24-h) and average real variability (ARV). Oscillometric PWV showed a positive correlation with all systolic BPV measures, in both groups. Among suspected hypertensives: 24-h SD, r = 0.30; SD daytime-SD, r = 0.34; nighttime-SD, r = 0.16; wSD, r = 0.30; CV 24-h, r = 0.24; ARV, r = 0.22. In the treated individuals: 24-h SD, r = 0.46; daytime-SD, r = 0.47; nighttime-SD, r = 0.35; wSD, r = 0.50; CV 24-h, r = 0.43; ARV, r = 0.37, all P < 0.001. Diastolic BPV demonstrated association with some measures of BPV. In suspected hypertensive group: nighttime-SD, r = 0.13; wSD, r = 0.10, both P < 0.001. And in treated individuals: daytime-SD, r = 0.23; wSD, r = 0.22; CV 24-h, r = 0.19 (all P < 0.001), ARV, r = 0.15 ( P < 0.05). Systolic daytime-SD in suspected and diastolic CV 24-h in treated group independently predicted oPWV. We observed a positive and independent correlation between Mobil-O-Graph pulse wave velocity and BPV measures, strong to systolic BPV and weak to diastolic BP.

Assessing the Spatial Distribution of Perfluorooctanoic Acid Exposure via Public Drinking Water Pipes Using Geographic Information Systems

Vieira, Veronica,Hoffman, Kate,Fletcher, Tony The Korean Society of Environmental Toxicology 2013 환경독성보건학회지 Vol.28 No.-

Objectives Geographic Information Systems (GIS) is a powerful tool for assessing exposure in epidemiologic studies. We used GIS to determine the geographic extent of contamination by perfluorooctanoic acid, C8 (PFOA) that was released into the environment from the DuPont Washington Works Facility located in Parkersburg, West Virginia. Methods Paper maps of pipe distribution networks were provided by six local public water districts participating in the community cross-sectional survey, the C8 Health Project. Residential histories were also collected in the survey and geocoded. We integrated the pipe networks and geocoded addresses to determine which addresses were serviced by one of the participating water districts. The GIS-based water district assignment was then compared to the participants' self-reported source of public drinking water. Results There were a total of 151,871 addresses provided by the 48,800 participants of the C8 Health Project that consented to geocoding. We were able to successfully geocode 139,067 (91.6%) addresses, and of these, 118,209 (85.0%) self-reported water sources were confirmed using the GIS-based method of water district assignment. Furthermore, the GIS-based method corrected 20,858 (15.0%) self-reported public drinking water sources. Over half (54%) the participants in the lowest GIS-based exposure group self-reported being in a higher exposed water district. Conclusions Not only were we able to correct erroneous self-reported water sources, we were also able to assign water districts to participants with unknown sources. Without the GIS-based method, the reliance on only self-reported data would have resulted in exposure misclassification.

Evolution of the solar irradiance during the Holocene

Vieira, L.E.A.,Solanki, S.K.,Krivova, N.A.,Usoskin, I. EDP Sciences 2011 Astronomy and astrophysics Vol.531 No.1

Dynamic analysis of bridge girders submitted to an eccentric moving load

Vieira, Ricardo F.,Lisi, Diego,Virtuoso, Francisco B. Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.1

The cross-section warping due to the passage of high-speed trains can be a relevant issue to consider in the dynamic analysis of bridges due to (i) the usual layout of railway systems, resulting in eccentric moving loads; and (ii) the use of cross-sections prone to warping deformations. A thin-walled beam formulation for the dynamic analysis of bridges including the cross section warping is presented in this paper. Towards a numerical implementation of the beam formulation, a finite element with seven degrees of freedom is proposed. In order to easily consider the compatibility between elements, and since the coupling between flexural and torsional effects occurs in non-symmetric cross-sections due to dynamic effects, a single axis is considered for the element. The coupled flexural-torsional free vibration of thin-walled beams is analysed through the presented beam model, comparing the results with analytical solutions presented in the literature. The dynamic analysis due to an eccentric moving load, which results in a coupled flexural-torsional vibration, is considered in the literature by analytical solutions, being therefore of a limited applicability in practice engineering. In this paper, the dynamic response due to an eccentric moving load is obtained from the proposed finite element beam model that includes warping by a modal analysis.

COLOR STABILITY OF THREE PACKABLE COMPOSITES (P-60, SOLITAIRE, SUREFIL) AND TWO UNIVERSAL COMPOSITES (CHARISMA, Z-250)

Vieira, Glauco Fioranelli,Morimoto, Susana,Arakaki, Yuri Dept. of Dental BiomaterialsCollege of Dentistry 2001 Dental materials journal, Seoul national universit Vol.11 No.1

Surface Extraction from Point-Sampled Data through Region Growing

Vieira, Miguel,Shimada, Kenji Society for Computational Design and Engineering 2005 International Journal of CAD/CAM Vol.5 No.1

As three-dimensional range scanners make large point clouds a more common initial representation of real world objects, a need arises for algorithms that can efficiently process point sets. In this paper, we present a method for extracting smooth surfaces from dense point clouds. Given an unorganized set of points in space as input, our algorithm first uses principal component analysis to estimate the surface variation at each point. After defining conditions for determining the geometric compatibility of a point and a surface, we examine the points in order of increasing surface variation to find points whose neighborhoods can be closely approximated by a single surface. These neighborhoods become seed regions for region growing. The region growing step clusters points that are geometrically compatible with the approximating surface and refines the surface as the region grows to obtain the best approximation of the largest number of points. When no more points can be added to a region, the algorithm stores the extracted surface. Our algorithm works quickly with little user interaction and requires a fraction of the memory needed for a standard mesh data structure. To demonstrate its usefulness, we show results on large point clouds acquired from real-world objects.

Sand-Nonwoven geotextile interfaces shear strength by direct shear and simple shear tests

Vieira, Castorina Silva,Lopes, Maria de Lurdes,Caldeira, Laura Techno-Press 2015 Geomechanics & engineering Vol.9 No.5

Soil-reinforcement interaction mechanism is an important issue in the design of geosynthetic reinforced soil structures. This mechanism depends on the soil properties, reinforcement characteristics and interaction between these two elements (soil and reinforcement). In this work the shear strength of sand/geotextile interfaces were characterized through direct and simple shear tests. The direct shear tests were performed on a conventional direct shear device and on a large scale direct shear apparatus. Unreinforced sand and one layer reinforced sand specimens were characterized trough simple shear tests. The interfaces shear strength achieved with the large scale direct shear device were slightly larger than those obtained with the conventional direct shear apparatus. Notwithstanding the differences between the shear strength characterization through simple shear and direct shear tests, it was concluded that the shear strength of one layer reinforced sand is similar to the sand/geotextile interface direct shear strength.

O corpo na narrativa da Batalha do Salado.

Vieira de Moura, Pedro 한국외국어대학교 외국종합연구센터 중남미연구소 2002 중남미연구 Vol.21 No.-