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Background : The advent of ubiquitous computing requires us to reconsider all aspects of industrial design engineering ? to invent, package and optimize such products, services and experiences to society. This project was devised to bridge these in a compelling and magical prototype, called the Kinetic Mirror, a mirror that can not only mimic color but also shape in front of it. It builds upon the efforts performed in the field of projector-based augmented reality and natural design interfaces, and it showcases our ideas of future prototyping of design concepts. Methods : This article describes the complexity of engineering when embodying and producing such interactive systems to disseminate design knowledge. Specifically, we reflect on the conceptualization and development of the Kinetic Mirror: a three-dimensional display that mirrors depth and color in 400 “pixels”. Enabled by the introduction of low-cost structured light sensors, we envisioned an instantaneous physical manifestation of the captured scan. Results : Challenges included: selecting electronic parts, software architecture, hardware and networking performance, outsourcing of production, power consumption, and overall assembly and construction The final system was put to show on five exhibits to test audience engagement and robustness of the result. This work has implications towards design curricula and provides new focal points of attention for design research and prototyping. Conclusions : Demonstration and prototypes are an increasingly important medium to disseminate design knowledge, because experience can only partly conveyed in written text or even in video. However, if products become dynamic, articulated and with behavior, the technology requirements for prototypes become more complex, and as a result harder to maintain. In this paper we shared our lessons learned.
CEBP, a nuclear-encoded chloroplast protein, has been previously cloned as a putative transcription factor involved in ethylene signaling in carnation development and senescence. In order to more clearly define CEBP role and function, we carried out experiments to define its pattern of mRNA abundance and possible subcellular localization. Changes in CEBP mRNA abundance showed a dramatic drop from anthesis to open flower stage of development immediately preceding the ethylene climacteric associated with flower senescence. A similar but less dramatic decrease in CEBP was observed upon ethylene exposure, again before endogenous climacteric ethylene was observed. The pattern of CEBP mRNA abundance suggests a response to or involvement in the initial steps of the petal senescence process. GFP co-localization showed that a GFP–CEBP construct was directed to the nucleus, whereas a CEBP–GFP construct localized to the chloroplast. Nuclear localization was expected as CEBP was cloned as an ethylene-responsive element-binding protein or transcription factor. The role and function of CEBP in the chloroplast, however, remains unclear. Future lines of inquiry based on our results are discussed.
A series of simulations were carried out to investigate the residual stress induced in the photovoltaic laminate during the cooling processafter lamination with a global model and several submodels. The simulations focus separately on the effects of the cooling rate, thecell layout and anisotropy on the residual stress and deformation of the photovoltaic laminate in a comparative manner with the finiteelement method. The results have shown that significant stress concentration and twist occurs in the interconnection region in the cell. Inaddition, different cooling rates, cell layouts and anisotropy only influence the largest stress rather than the stress distribution and deformation. Therefore, the results of a uniform stationary isotropic model with fewer cells can provide enough insight into the stress distributionin real photovoltaic laminates and the modified largest first principal stress can be used for design and verification.