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Verónica García-Villamar,Laura G. Hernández-Aragón,Jesús R. Chávez-Ríos,Arturo Ortega,Margarita Martínez-Gómez,Francisco Castelán 대한배뇨장애요실금학회 2018 International Neurourology Journal Vol.22 No.S2
Purpose: To evaluate the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptor, GDNF family receptor alpha subunit 1 (GFRα-1) in the pelvic (middle third) vagina and, particularly, in the paravaginal ganglia of nulliparous and primiparous rabbits. Methods: Chinchilla-breed female rabbits were used. Primiparas were killed on postpartum day 3 and nulliparas upon reaching a similar age. The vaginal tracts were processed for histological analyses or frozen for Western blot assays. We measured the ganglionic area, the Abercrombie-corrected number of paravaginal neurons, the cross-sectional area of the neuronal somata, and the number of satellite glial cells (SGCs) per neuron. The relative expression of both GDNF and GFRα-1 were assessed by Western blotting, and the immunostaining was semiquantitated. Unpaired two-tailed Student t -test or Wilcoxon test was used to identify statistically significant differences (P≤0.05) between the groups. Results: Our findings demonstrated that the ganglionic area, neuronal soma size, Abercrombie-corrected number of neurons, and number of SGCs per neuron were similar in nulliparas and primiparas. The relative expression of both GDNF and GFRα- 1 was similar. Immunostaining for both GDNF and GFRα-1 was observed in several vaginal layers, and no differences were detected regarding GDNF and GFRα-1 immunostaining between the 2 groups. In the paravaginal ganglia, the expression of GDNF was increased in neurons, while that of GFRα-1 was augmented in the SGCs of primiparous rabbits. Conclusions: The present findings suggest an ongoing regenerative process related to the recovery of neuronal soma size in the paravaginal ganglia, in which GDNF and GFRα-1 could be involved in cross-talk between neurons and SGCs.
Verónica Perea,Xavier Urquizu,Maite Valverde,Marina Macias,Anna Carmona,Esther Esteve,Gemma Escribano,Nuria Pons,Oriol Giménez,Teresa Gironés,Andreu Simó-Servat,Andrea Domenech,Núria Alonso-Carril,Car 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.6
Background: This study aimed to evaluate the influence of maternal diabetes in the risk of neurodevelopmental disorders in offspring in the prenatal and postnatal periods.Methods: This cohort study included singleton gestational diabetes mellitus (GDM) pregnancies >22 weeks’ gestation with live newborns between 1991 and 2008. The control group was randomly selected and matched (1:2) for maternal age, weeks of gestation and birth year. Cox regression models estimated the effect of GDM on the risk of attention-deficit/hyperactivity disorder (ADHD), autism spectrum disorder (ASD), and maternal type 2 diabetes mellitus (T2DM). Moreover, interaction between maternal T2DM and GDM-ADHD relationship was evaluated.Results: Children (<i>n</i>=3,123) were included (1,073 GDM; 2,050 control group). The median follow-up was 18.2 years (interquartile range, 14.2 to 22.3) (<i>n</i>=323 with ADHD, <i>n</i>=36 with ASD, and <i>n</i>=275 from women who developed T2DM). GDM exposure was associated with ADHD (hazard ratio [HR]<sub>crude</sub>, 1.67; 95% confidence interval [CI], 1.33 to 2.07) (HR<sub>adjusted</sub>, 1.64; 95% CI, 1.31 to 2.05). This association remained significant regardless of the treatment (diet or insulin) and diagnosis after 26 weeks of gestation. Children of mothers who developed T2DM presented higher rates of ADHD (14.2 vs. 10%, <i>P</i>=0.029). However, no interaction was found when T2DM was included in the GDM and ADHD models (<i>P</i>>0.05). GDM was not associated with an increased risk of ASD (HR<sub>adjusted</sub>, 1.46; 95% CI, 0.74 to 2.84).Conclusion: Prenatal exposure to GDM increases the risk of ADHD in offspring, regardless of GDM treatment complexity. However, postnatal exposure to maternal T2DM was not related to the development of ADHD.
Liliana Mabel Gerard,Cristina Verónica Davies,Carina Alejandra Soldá,María Belén Corrado,María Verónica Fernández 한국미생물·생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.2
Sixteen acetic acid bacteria (AAB) were isolated from blueberries and citric fruits of the Salto Grande region (Concordia, Entre Ríos, Argentina) using enrichment techniques and plate isolation. Enrichment broths containing ethanol and acetic acid enabled maximum AAB recovery, since these components promote their growth. Biochemical tests allowed classification of the bacteria at genus level. PCR-RFLP of the 16S rRNA and PCR-RFLP of the 16S-23S rRNA intergenic spacer allowed further classification at the species level; this required treatment of the amplified products of 16S and 16S-23S ITS ribosomal genes with the following restriction enzymes: AluI, RsaI, HaeIII, MspI, TaqI, CfoI, and Tru9I. C7, C8, A80, A160, and A180 isolates were identified as Gluconobacter frateurii; C1, C2, C3, C4, C5, C6, A70, and A210 isolates as Acetobacter pasteurianus; A50 and A140 isolates as Acetobacter tropicalis; and C9 isolate as Acetobacter syzygii. The bacteria identified by 16S rRNA PCR-RFLP were validated by 16S-23S PCR-RFLP; however, the C1 isolate showed different restriction patterns during identification and validation. Partial sequencing of the 16S gene resolved the discrepancy.
Chymotrypsin - Eudragit Complex Formation
Valeria Boeris,Laura Verónica Cappella,Gisele Peres,Inés Burgos,Nádya Pesce da Silveira,Gerardo Fidelio,Guillermo Picó 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
Eudragit® L100 (EuL) and Eudragit® S100(EuS) are synthetic polyanions differing on their electric charge density. They interact with chymotrypsin (ChTRP),a basic protein forming soluble and non-soluble complexes. The complex formation was studied by dynamic light scattering, isothermal titration calorimetry, native fluorescence emission, circular dichroism and thermodynamical thermal stability of the enzyme. EuS was able to bind 33 ChTRP molecules while EuL, 60. The binding of ChTRP to both Eu was slightly endothermic and the entropic factor was responsible for the soluble complexes formation. The ChTRP-Eu size increases with pH and the binding of ChTRP to Eu modifies the Eu hydrodynamic radium. The interaction of ChTRP with Eu did not modify its secondary or tertiary structure. The thermal stability of ChTRP was increased when it interacted with both Eu.
Lilian de Siqueira,Verônica Ribeiro dos Santos,Juliani Caroline Ribeiro de Araújo,Hugo Gutemberg Patiño de Oliveira Filho,Luana Marotta Reis de Vasconcellos,Eliandra de Sousa Trichês,Alexandre Luiz So 한국섬유공학회 2023 Fibers and polymers Vol.24 No.9
The development of new biomaterials with improved properties is a trend in tissue regeneration. In this way, an innovative approach is employed in this work for obtaining polymer fibers coated with nanoparticles resulting from the simultaneous application of poly (lactic acid) (PLA)/polycaprolactone (PCL) electrospinning and bioactive particles from an amorphous multicomponent silica–calcium–phosphorus system (AMS) electrospraying. The osteogenesis was evaluated in vitro and in vivo using male rats, in which total protein, alkaline phosphatase, and biological performance through histological and histomorphometric analysis were discussed. The morphological results assessed by scanning electron microscopy showed a mesh of PLA/PCL fibers associated with AMS. The spraying of 17.44% of AMS particles in the PLA/PCL electrospun fibers decreased the Young’s modulus and tensile strength. However, the amount of AMS particles sprayed was enough to promote a reduction of 17.8% in the measured contact angle values. Phosphatase alkaline higher mean value was also observed in the fibers when in contact with the AMS, but nonstatistical difference was observed (p > 0.05). It was possible to observe the presence of mineralized nodules deposited on the bottom of the plate and between the fibers. The newly formed bone into defect filled with PLA/PCL-AMS fibers was higher than that observed in the control group. These findings suggest PLA/PCL-AMS fibers as a multifunctional composite system that may be attractive for both bone and dental tissue engineering applications.
Chronic Toxicity, Genotoxic Assay, and Phytochemical Analysis of Four Traditional Medicinal Plants
América Castañeda Sortibrán,María Guadalupe Ordaz Téllez,Verónica Muñoz Ocotero,Marco Antonio Carballo-Ontiveros,Angélica Méndez García,Rocio Jimena Jiménez Valdés,Elizabeth Romero Gutiérrez,Rosario R 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.9
Four medicinal plants—Tecoma stans, Ligusticum porteri, Monarda austromontana, and Poliomintha longiflora, which are distributed in tropical and subtropical countries of the American continent—are widely used in folk medicine to treat diseases such as diarrhea and dysentery. In addition, T. stans and P. longiflora are extensively used as hypoglycemic agents, and M. austromontana and P. longiflora are used as condiments. The plants were collected, identified, dried, and pulverized. Solvent extraction was prepared by maceration of the plant samples, and the phytochemical composition of the extracts was determined by using standard analysis procedures. Phytochemical analysis showed the presence of triterpenoids/steroids, flavonoids, and phenols/tannins and, in L. porteri, traces of alkaloids. After the elimination of solvents in vacuo, the extracts were administrated to Drosophila larvae to test their toxicity and genotoxicity. Third instar larvae were chronically fed with the phytoextracts. The extract from L. porteri was toxic, whereas those from T. stans, P. longiflora, and M. austromontana were not. Genotoxic activities of the 4 plants were investigated by using the wing-spot assay of D. melanogaster. Mitomycin C was used as a positive control. No statistically significant increase was observed between treated sample series and a concurrent negative (water) or solvent control sample series.