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Varnika Bhatia,Amit Alok 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.3
A post-transfer investigation was carried out to analyze the process of gene introgression in a conventional backcross-breedingprogram taking low erucic acid as target trait in Brassica juncea. FAE1 locus is involved in the elongation of oleic acid (C18:1) to erucicacid (C22:1). A high concentration of erucic acid in the seeds of Brassica species has been reported to be nutritionally undesirable. Molecular markers like AFLP markers, microsatellite markers, and gene-based SNP markers were used to determine the size of thedonor parent chromosomal segments retained around the FAE1 genes in the individuals selected from different backcross generations. The genotype of the individuals was inferred from the genotype of the markers and the graphical genotypes were constructed usingGGT software. Molecular marker analysis led to the identification of rare recombinants near the target locus with reduced size of introgressedsegment from the donor parent. Based on the present study, we propose that marker-assisted backcross breeding in B. junceacould prove to be a promising tool for the transfer of many quality traits from unadapted East European germplasm to Indian cultivars
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