여주 건조분말 함유식이가 고콜레스테롤 혈증 Rat의 혈청성분에 미치는 영향

이웅수 忠州大學校 2007 한국교통대학교 논문집 Vol.42 No.-

This research was carried out to investigate the hypocholesterolemic effects of diets containing bitter gourd(Momordica charantia, MC; Yeoju) powder in Sprague-Dawley male rats. After 2 weeks feeding with basal diet, 6 week old rats were fed with basal diet, diets containing 1% cholesterol, and 1% MC, 5% MC and 10% MC powder with 1% cholesterol for 2, 4 and 6 week. And then I measured the levels of glucose, triglyceride, total-, HDL-and LDL-cholesterol, and the activities of GOT, GPT in the serum of rats. The levels of triglyceride and total-cholesterol in the serum of rats were decreased remarkably in the groups fed with diet contained more 5% MC powder compared to the basal diet group for 2weeks. The levels of LDL-cholesterol were decreased in the all groups fed with diet contained MC power for 4weeks, especially groups fed with contained more 5% MC powder were more effective than the other groups, but the decrease of HDL-cholesterol level was not effective in all diet groups contained MC powder. The levels of blood sugar were decreased in all MC powder diet groups, and the decreased levels were more effective in the groups fed with diet contained more MC powder. The activities of GOT and GPT were also decreased in all MC powder diet groups. In conclusion, diets contained MC powder were effective in the decrease of triglyceride, total- and LDL-cholesterol, and glucose levels, and GOT and GPT activities in the serum of rats

설사환자의 대변에서 분리한 세균성 병원체의 특성 조사 II. Staphylococcus aureus의 분리·동정 및 항생제에 대한 내성 조사

이웅수 忠州大學校 2010 한국교통대학교 논문집 Vol.45 No.-

I investigated the characterization of bacterial pathogens on 2,270 stool specimens, which was collected from dysentery patients for one year, 2007 Detection of enteric bacteria in stool of diarrheal patient was performed according to the procedure of routine protocol such as culture and multiplex PCR method. Biochemical test for the identification of Staphylococcus aureus(S. aureus) was performed by API Staphy kit. In order to cofirm of toxin type, I also carried out serological test. Among 456 isolates of bacterial pathogen isolated from the collected stool, S. aureus was 74 isolates(toxin A type 16, C type 9, D type 1, non-toxin type 48). Detection rate of S. aureus in dysentery patients was the higher in April, August and December, and in children below 10 years old(66.2%). In addition, S. aureus in male was isolated higher rate than their in female. Antibiotics resistance rate on the each toxin type S. aureus showed that type A is AM > PC > EM, OC > TC > CP > CT > CM > GM, IP > CI > ChP > TM/SM, RP, and type C is AM, PC, EM, OC > CP, CM, TC > CT, CI, IP > GM > TM/SM, RP, and type D is only 100% on AM, PC and TC. In the present study, I focused to investigate the incidence and antibiotics resistance patterns in each toxin type S. aureus related diarrheal patients. These results were very helpful to make preventive method for infectious disease causing bacterial gastroenteritis, especially, food poisoning by enteric bacterial infection.

장내세균에 의한 Trichothecene Mycotoxins의 대사 : (I) T-2 toxin의 대사

이웅수 忠州大學校 2008 한국교통대학교 논문집 Vol.43 No.-

Trichothecene mycotoxins are a chemically related group of toxic fungal metabolites produced by a number of species of the fungi such as Fusarium, Cephalosporium, Trichothecium, Myrothecium, Stachybotrys and Trichoderma, and are responsible for mycotoxicoses as causative agents in a wide variety of animal and human health problems. Especially, T-2 toxin is a secondary metabolite produced by Fusarium spp. such as F. tricinctum, F. poae and F. sporotrichioides etc., and is often found in agricultural products and feeds including cereals, and is a potent cytotoxic and immunodepressive trichothecene mycotoxin, and causative agent of moldy corn toxicosis and alimentary toxic aleukia(ATA), and induces acute toxicity such as leukocytosis, hemorrhage and dermal edema, followed by death in animals. In order to elucidate the possible metabolism of T-2 toxin by rat and human intestinal bacteria, this research was carried out. T-2 toxin transformed into HT-2 toxin and unknown metabolite by cultured intestinal bacteria in the anaerobic condition. The amount of HT-2 toxin was decreased, but unknown metabolite was increased by subsequent incubation. This metabolite was elucidated to deepoxy HT-2 toxin by NMR and mass spectra data. It was suggested that T-2 toxin was deepoxidized after deacetylation to HT-2 toxin by the intestinal bacteria. This deepoxy HT-2 toxin was transformed into HT-2 toxin and 15-deacetyl-deepoxy HT-2 toxin by liver microsome of PCB-treated rat in the presence of NADPH and oxygen. These results suggest that the epoxide group of trichothecene mycotoxins is reduced by intestinal bacteria and re-oxidized by hepatic microsomal enzyme of rat.

配當과 株價에 關한 硏究 : 一部上場 企業을 中心으로

朴秀雄 경성대학교 1982 부산수산대학 논문집 Vol.3 No.1

Fusarium 속 곰팡이가 생산하는 Mycotoxin인 Nivalenol의 발암성에 관한 연구

李雄洙 충주대 산업과학기술연구소 2000 産業科學論文集 Vol.8 No.-

Nivalenol(NIV), a trichothecene mycotoxin produced by Fusarium spp., was significantly contaminated to cereals such as barley, polished barley, wheat, malt and corn, and animal feeds at high level and high frequency throughout the world. Therefore, I was carried out this research to evaluate the toxicological effects especially carcinogenicity of NIV in mice during 2 years feeding trial. In 2 years feeding period, the survival rates were 52, 52, 48 and 69% in the groups given 0, 6, 12 and 30ppm NIV, respectively. Lethal case was not observed in the 30ppm group until 18 months feeding period. Mean survival days in each group for 2 years feeding period were 565, 629, 654 and 719 days. These results indicated the increase of life span in the NIV-treated groups. The incidence rates of total tumor after 2 years feeding trial were 50, 66, 59 and 56% in each groups, respectively. Most of tumors observed in control and NIV-treated groups were lymphomas which spontaneously occurred at high frequency. There was no significant difference in the tumor incidence rate between control and NIV-treated groups. The results of this research showed that NIV was not carcinogenic substance, and reversely increased life scan according to the concentration of NIV in mice.

Anthraquinone계 화합물의 변이원성 및 유전독성 발현에 관한 연구(I) : Mutagenicity of emodin activated with cytochrome P-450 Cytochrome P-450에 의해 활성화된 emodin의 변이원성

李雄洙 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.2

It has been reported that several anthraquinone compounds possess mutagenicity and carcinogenicity. Emodin is one of the anthraquinone mycotoxins, and a constituent of several herb medicine used as laxative such as Rhei Rhizoma(rhubarb), Cassiae Semen(cassia seed) and Aloe. Emodin was selected as a model compound to solve a carcinogenic mechanism of the anthraquinone mycotoxins and to evaluate the safety of herb medicine. In this research, the mutagenicity and metabolic reaction of emodin were investigated with the purified cytochrome P-450s. In Ames test with the reconstituted cytochrome P-450 system derived from the hepatic microsomes of PB-induced rats, the mutagenicity of emodin activated with the fractionⅡ(P-448 type) was significantly higher than with the fraction Ⅰ(P-450 type). TLC analysis of the metabolites revealed that among 8 isozymes of P-450 type(Ⅰ-a, b, c, d) and P-448 type(Ⅱ-a, b, c, d), only ω-OH-emodin was detected on the TLC plate when emodin was incubated with 4 isozymes of P-450 type(Ⅰ-a, b, c, d). Whereas, 2-, 4-, 7-, ω-OH-emodin were detected in the metabolites produced by P-448 type(Ⅱ-a, b, c, d). Of 8 isozymes, Ⅱ-a possessed the highest activity in the production of 2-OH-emodin, a direct-acting mutagen. This Ⅱ-a was also represented to catalyze the hydroxylation of aflatoxin B1 and ochratoxin A into aflatoxin M1 and 4(R)-4-hydroxy-ochratoxin A, respectively. It was suggested that P-448 types play an important role in the metabolic activation of emodin.

동물물리치료

김진웅,배수찬 대한정형물리치료학회 2002 대한정형도수물리치료학회지 Vol.8 No.2

This article review the animals physical therapy. It is so called veterinary physical therapy. The animals physical therapist is working to physical therapy for animals in veterinary clinical field. The veterinary medicine has not veterinary rehabiliation medicine or physical therapy for animals, also physical therapy field in Korea. So, This research will explain about animals physical therapy of Korea and other countries' by journals and internet information and suggest the future of the animals physical therapist. Finally, The veterinary physical therapy is not used to veterinary clinical field in now. But, The veterinary physical therapy will desire to the view of medical serve and economy by the host of animals and clinical veterinarian the future. Animal physical therapy is a new and rapidly developing field of health care for animals. The benefits of physical therapy have long been recognized in humans. More recently, work in the veterinary field has shown the same benefits of physical therapy to be true for animal patients. Performing orthopaedic or neurological surgery, or fitting a human patient with a cast or splint, and then discharging the patient is an outdated approach. In such cases, physical therapy is clearly warranted. Similarly, recent research has shown that post-surgical rehabiltation and therapy after injuries significantly improves the functional outcomes for animals. Physical Therapy is a healthcare profession directed at evaluating, restoring and maintaining physical function and movement. Working with the owner, veterinarian and often other healthcare professionals, a physiotherapist helps your animal to achieve and maintain optimal health and well-being. Equipped with a specialized university Bacheloriate education and intensively educated in Anatomy, Physiology, Biomechanics, Histology, Neurology, and Pathology, PT's are able to assess, diagnose and treat movement and function. Physical Therapy, Sports Medicine and Rehabilitation are recognized sciences applied to both humans and animals. The goals of physiotherapy are to relieve pain, restore range of motion/movement, improve function, prevent injuries and expand the physical potential of the patient. Once in the field, physical therapists actively continue their education to keep up to date on the latest treatments and technologies. Via continuing education courses, physiotherapists can learn how to apply their unique and specialized knowledge to other animal species.

非營利組織의 會計制度에 관한 考察 : 企業會計制度의 導入을 중심으로

田雄秀 단국대학교 산업연구소 1985 산업연구 Vol.8 No.-

여성 생식계조직의 각종 임신연관 혈장단백의 분포에 관한 면역효소조직화학적 연구

남웅수,이규완,홍성봉 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3

Pregnancy associated plasma protein-A(PAPP-A), pregnancy-specific β-1 glycoprotein (SP1) and human placental lactogen(HPL), which are specific protein, have been known to be detectable in the plasma of pregnant women, especially in mid and last trimester by radioimniunoassay. Various researches have been reported on these and the increments of them could be of value as the tumor markers and as the pregnancy related abnormalities. Surgical specimens of normal and pathologic female genital tissues were fixed in formalin and embedded in paraffin in order to detect the exact distribution of PAPP-A, SP1 and HPL in various tissue component of them. The result obtained are as follows: 1. Normal superficial and intermediate squamous epithelial cells, moderate and severe dysplasia, carcinoma in situ and keratinizing squamous cell carcinoma of uterine cervix revealed positive reaction to anti PAPP-A, SP1, and HPL antibodies. 2. Endometrium in both proliferative and secretory phase, endometrial hyperplasia, trophoblastic tumors revealed positive reaction to anti PAPP-A, SP1, and HPL antibody, hut endometrial adenocarcinoma revealed negative reaction to each of them. 3. All of the normal ovarian tissues and various epithelial ovarian tumors revealed negative reaction to PAPP-A, SP1 and HPL antibody, but stromal and germ cell ovarian tumors revealed positive reaction to each of them. 4. Reactions to each PAPP's were in uniformity among the same tissues, that is, once the reaction was positive, the reactions to the other remaining two PAPP's were positive. Similarly negative reactions appeared in the same fashion.

흰쥐에서 녹차카테킨류의 간추출율의 평가

임재수,황성주,지웅길,김동출 충남대학교 약학대학 의약품개발연구소 2002 藥學論文集 Vol.17 No.-

To investigate the role of liver in the elimination of green tea catechins, the hepatic extraction ratio of epigallocatechin gallate, epigallocatechin, and epicatechin gallate was investigated following intraduodenal administration of green tea catechin extracts in Sprague-Dawley rats. The plasma concentrations of the catechins in portal and systemic circulations were determined simultaneously in the same rat to evaluate the hepatic extraction ratio. The values of hepatic extraction ratio of epigallocatechin gallate, epicatechin gallate, and epicatechin were 0.54, 0.28, and 0.51, respectively. Therefore, it is suggested that the hepatic clearance plays a major role in the elimination of epigallocatechin gallate and epicatechin.