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Tushar Sharma,G. Suresh Kumara,전보현,Jitendra S. Sangwai 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.22 No.-
Pickering emulsion offers potential applications in several fields including oil and gas industry due totheir enhanced stability. Oil-in-water (o/w) emulsions are usually stabilized by surfactant ornanoparticle or by both but show poor thermal stability which limits their use for high-temperatureapplications. In this work, a novel formulation of o/w emulsion stabilized using nanoparticle-surfactantpolymersystem is investigated for the formulation of thermally stable Pickering emulsion. Theconventional oilfield polymer polyacrylamide (PAM), surfactant, sodium dodecylsulfate (SDS), andnanoparticles such as, SiO2, clay, and CuO in varying concentration are used. It is observed that thenanoparticle in the presence of surfactant and polymer synergistically interacted at the oil–waterinterface. The effect of temperature, pH, and salinity on the interfacial tension is investigated tounderstand the thermal stability. The emulsion system with partially hydrophobic clay nanoparticles inthe presence of PAM and SDS shows higher thermal stability as compared to fully hydrophilic SiO2nanoparticles. In the presence of salt, NaCl (1.0 wt%), the thermal stability of clay and SiO2 stabilizedemulsions is observed to be further promoted at higher temperatures. Scanning electron microscopy(SEM) images confirm the existence of a structured and rigid layer of nanoparticle at the oil–waterinterface.
Experimental Study of Thickness Gradient and Flow Simulation in VARTM Process
Tushar Gajjar,Dhaval B. Shah,S. J. Joshi,K. M. Patel 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2
Vacuum assisted resin transfer moulding (VARTM) process is widely used for manufacturing composite products. It is very difficult to manufacture a composite component with small dimension tolerance using VARTM process. In this research paper, carbon fiber reinforced polymer (CFRP) flat plates have been manufactured using the VARTM process with a different combination of process parameters. The carbon fiber laminates with different thickness have been manufactured using the VARTM process. The thickness of manufactured components has been measured at different locations using noncontact type 3D scanning techniques. The warpage has been determined for each manufactured components. The compaction pressure has been measured using pressure gauge at a different location during manufacturing. The thickness variation, pressure variation and volume fraction have been determined across the length for each manufactured components for different thickness laminates. The mould filling time has been calculated using the numerical approach in ANSYS Fluent software and the same has been compared with experimental results. The percentage difference between the experimental and numerical approach has been found within 5 %.
A two stroke free piston engine's performance and exhaust emission using artificial neural networks
Tushar Ahmed,임옥택 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.10
The performance and exhaust emissions of a Free piston linear engine (FPLE) were ascertained for various equivalence ratios (0.7, 0.8, 0.9, 1.0, 1.1 and 1.2). After that, the usability of Artificial neural networks (ANNs) in case of FPLE has been tested. Actually, the aim was to examine the best suited operational condition of, and show the possibility of using ANN, for this kind of engine technology. We first interrogated the thermal efficiency, generated power, total heat release rate, indicated mean effective pressure, exhaust gas temperature, and exhaust emissions such as CO, CO 2 , NO x and O 2 for the chosen range of equivalence ratios and then gathered experimental data in order to train and test an artificial neural network model for prediction. The experimental results showed that, running the engine on the slightly lean side stoichiometrically, can fulfil the goals of higher engine performance and lower emissions. We used the back propagation learning algorithm for ANN and observed that the correlation coefficients (R) vary between 0.990-0.999, Mean absolute percentage error (MAPE) vary between 0.885-5.9%, and coefficient of determination (R 2 ) vary between 0.937-0.999; which showed a welldefined relationship between the predicted and experimental values.
Tyrosine 1045 Codon Mutations in Exon 27 of EGFR are Infrequent in Oral Squamous Cell Carcinomas
Tushar, Mehta Dhaval,Ramanathan, Arvind Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7
Background: The activation and inactivation of receptor tyrosine kinases are tightly regulated to ensure faithful replication of cells. After having transduced extracellular growth activating signals, activated EGFR is subjected to downregulation either by clathrin mediated endocytosis or c-Cbl mediated proteasome degradation depending on the ligand concentration. c-Cbl is an ubiquitin ligase which requires a phosphorylated tyrosine residue at position 1045 in the cytoplasmic domain of EGFR to interact and add ubiquitin molecules. While activating mutations in exons 19 and 21 have been associated with the development of several cancers, the status of mutations at tyrosine 1045 coding exon 27 of EGFR remain to be investigated. Consistently, defective phosphorylation at 1045 has been associated with sustained phosphorylation of EGFR in non-small lung carcinomas. Hence in the present study we investigated the genetic status of the tyrosine 1045 coding site within exon 27 of EGFR gene to explore for possible occurrence of mutations in this region, especially since no studies have addressed this issue so far. Materials and Methods: Tumor chromosomal DNA isolated from thirty five surgically excised oral squamous cell carcinoma tissues was subjected to PCR amplification with intronic primers flanking the tyrosine 1045 coding exon 27 of EGFR gene. The PCR amplicons were subsequently subjected to direct sequencing to elucidate the mutation status. Results: Sequence analysis identified no mutations in the tyrosine 1045 codon of EGFR in any of the thirty five samples that were analyzed. Conclusions: The lack of identification of mutation in the tyrosine 1045 codon of EGFR suggests that mutations in this region may be relatively rare in oral squamous cell carcinomas. To the best of our knowledge, this study is the first to have explored the genetic status of exon 27 of EGFR in oral squamous cell carcinoma tissue samples.
Tushar Ramdas Sabale,Pranav Pradip Kulkarni,Anand Rameshchandra Ghosalkar 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.4
Poly-3-hydroxybutyrate (PHB) is a bio-based and biodegradable polymer produced by microbial fermentation from wide variety of feedstocks. Methanotrophic organism Methylosinus trichosporium has been reported for the production of PHB using alternate feedstock like methane which is economical and abundantly available. PHB was produced from methane by cultivating M. trichosporium in a bioreactor with continuous cell recycle system. Different gas sparging strategies were evaluated for impact on cell biomass and PHB production. The fermentation was conducted in different modes including batch and continuous culture with and without membrane recycle. Using membrane based recycle system the biomass increased from 1.5 to maximum of 7.31 g DCW/L in 27 days. Although PHB content was comparable in both batch mode and membrane based recycle system, a 4.9 fold increase in biomass production enhanced the PHB titer by 6.1 fold as compared to batch culture. Continuous cell recycle processes provide an opportunity to increase the competitiveness of gas fermentation based processes for methanotrophs based PHB production.
Tushar Sharma,Jitendra S. Sangwai,G. Suresh Kumara 한국자원공학회 2014 Geosystem engineering Vol.17 No.3
A conventional waterflood often leads to unsuccessful recovery of oil, as most of the injected water tends to channel into the more permeable zones. Pickering emulsions stabilized using surfactant and colloidal particles, such as nanoparticles, are gaining wider recognition in the petroleum industry due to their better thermal stability and stabilized flow behavior. In this work, a novel formulation of oil-in-water (o/w) emulsion stabilized using nanoparticle–surfactant–polymer in the presence of salt (NaCl) is investigated for improved oil recovery at conditions of high pressure (13.6MPa) and high temperature (313–363 K).We report a comparative study of performance of o/w emulsion flooding with conventional water flooding for enhanced oil recovery of a crude oil having a viscosity of 161 mPa s at 313K in a Berea sandstone core using core-flood experiments. The results of core flooding tests show that an incremental oil recovery of more than 23% of original oil in place over water flooding can be obtained using an emulsion flooding formulated in this work.