Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl‑1 with yellow–green leaf color

Tong‑Mei Gao,Shuang‑Ling Wei,Jing Chen,Yin Wu,Feng Li,Li‑Bin Wei,Chun Li,Yan‑Juan Zeng,Yuan Tian,Dong‑Yong Wang,Hai‑Yang Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.1

Background Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops. Objective This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow–green leaf sesame mutant Siyl-1. Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type. Results Genetic analysis indicated that there were 3 phenotypes of the F2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellowgreen mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P < 0.05). The 2-DE comparison showed that there were 98 differentially expressed proteins (DEPs) among YY, Yy, and yy lines. All the 98 DEPs were classified into 5 functional groups. Of which 82.7% DEPs proteins belonged to the photosynthesis and energy metabolism group. Conclusion The results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame.

Anatomic characteristics and novel transplantation model of the canine uterus

Xuan-Hai Do,Thanh-Hai Tong,Trung-Chuc Nguyen,Tuan-Anh Ngo,Minh-Trang Thi Tran 대한이식학회 2023 Korean Journal of Transplantation Vol.37 No.2

Background: In Vietnam, the rate of absolute uterine factor infertility is increasing, but no study has been published on uterine transplantation. The present study was designed to comprehensively observe the canine uterine anatomy and to examine the possibility of using a living canine donor for uterine transplantation training and further research. Methods: Ten female Vietnamese mixed-breed dogs were sacrificed for anatomical research, and 15 additional pairs were used to evaluate the novel uterine transplant model. Results: The anatomic features of the canine uterus differed considerably from those of the human uterus, with the uterine vessels originating from branches of the pudendal vessels (also known as the vaginal vessels). The uterine vascular pedicle had a small diameter (1 to 1.5 mm for arteries and 1.2 to 2.0 mm for veins) and required manipulation under a microscope. To perform uterine transplantation, the donor specimen’s artery and vein lengths were successfully reconstructed by anastomosis between both sides of the vasculature using autologous Y-shaped subcutaneous veins. The living-donor uterine transplantation model constructed in this study was feasible, with the transplanted uterus surviving in 86.7% of cases (13/15). Conclusions: Uterine transplantation was successfully performed in a Vietnamese canine living donor model. This model could be helpful in uterine transplantation training and improve the transplantation success rate in humans.

Multiuser Diversity based Adaptive Scheduling Scheme for OFDMA systems

Giang Hai Tong,HyeongJung Kim,Subodh Pudasaini,Seokjoo Shin 한국차세대컴퓨팅학회 2012 한국차세대컴퓨팅학회 논문지 Vol.8 No.1

OFDMA 기반 다중사용자 시스템에서는 상향링크 피드백 형식으로 기지국에 보고되는 각 사용자의 채널 품질 정보(CQI)를 기반으로 사용자를 선택한다. 이를 위해 상향링크 채널의 오버헤드가 많이 요구되는 상세한 피드백 정보를 이용하거나 오버헤드는 적지만 불충분한 CQI 정보를 이용하는 방식은 상호 trade-off 관계에 있기 때문에 시스템 처리률을 최적화시키기에 어려움이 있다. 따라서 이 문제를 해결하기 위해, best-M 스케줄링 기법이 제안되었다. best-M 기법에서는 모든 서브채널에 대해서 CQI를 전송하는 대신에 M 서브채널의 CQI 만을 기지국으로 전송함으로써 상향링크 오버헤드를 줄일 수 있게 된다. 본 논문에서는 시스템의 처리량을 더 향상시키기 위해서, 능동적 사용자들의 수를 기반으로 M을 적응적으로 계산하는 적응적 best-M 피드백 스케줄링 방식을 제안하였다. 컴퓨터 시뮬레이션을 통한 제안 방식의 성능 분석 결과로부터 제안된 기법은 상향링크 오버헤드를 현저히 줄이면서 향상된 하향링크 처리률을 제공할 수 있음을 확인하였으며, 특히 best M 방식과 비교할 경우 동일 시스템에서 더 많은 동시 사용자를 지원할 수 있음을 확인하였다. 또한, 본 연구에서는 총 피드백 오버헤드 제한 조건에 따른 시스템 최적 처리률을 얻기 위한 중요 동작 포인트를 얻을 수 있었다.

Transcriptional analysis of Pieris rapae in response to P. rapae granulovirus

Hai-Jian Huang,Tong-Qiang Zhang,Qiao Lin, Jian-Hui Ye,Chuan-Xi ZHANG,Bao-Qin Zhang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2

Pieris rapae granulovirus (PrGV) is an important pathogen that has been exploited as a microbial insecticide to control agriculture pests. They can specifically infect cabbage butterfly (Pieris rapae), causing a series of pathological symptoms. In this infected P. rapae at 6 h and 72 h. As a result, a series of host genes were significantly modulated following PrGV infection, including those correlated with exoskeleton, ribosome, heat shock protein (HSP), proteasome, oxidation-reduction and apoptosis. Taken together, our study unveiled the P. rapae response to PrGV at different time point and provided a potential strategy for pest management.

Hypertriglyceridemia: A Neglected Risk Factor for Ischemic Stroke?

Hai-jie Liang,Qing-yi Zhang,Yi-tong Hu,Guo-qing Liu,Rong Qi 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.1

Hypertriglyceridemia is caused by defects in triglyceride metabolism and generally manifests as abnormally high plasma triglyceride levels. Although the role of hypertriglyceridemia may not draw as much attention as that of plasma cholesterol in stroke, plasma triglycerides, especially nonfasting triglycerides, are thought to be correlated with the risk of ischemic stroke. Hypertriglyceridemia may increase the risk of ischemic stroke by promoting atherosclerosis and thrombosis and increasing blood viscosity. Moreover, hypertriglyceridemia may have some protective effects in patients who have already suffered a stroke via unclear mechanisms. Therefore, further studies are needed to elucidate the role of hypertriglyceridemia in the development and prognosis of ischemic stroke.

Self-Identity of the Passively Urbanized Group in the Process of Obtaining Urban Adaptability and Modernity: An Empirical Study on 561 Land-Displaced Peasants in Nanjing

Hai Bo Zhang,Xing Tong 전남대학교 지역개발연구소 2008 지역개발연구 Vol.40 No.2

The purified extract of steamed Panax ginseng protects cardiomyocyte from ischemic injury via caveolin-1 phosphorylation-mediating calcium influx

Hai-Xia Li,Yan Ma,Yu-Xiao Yan,Xin-Ke Zhai,Meng-Yu Xin,Tian Wang,Dong-Cao Xu,Yu-Tong Song,Chun-Dong Song,Cheng-Xue Pan 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Background: Caveolin-1, the scaffolding protein of cholesterol-rich invaginations, plays an important rolein store-operated Ca2þ influx and its phosphorylation at Tyr14 (p-caveolin-1) is vital to mobilize protectionagainst myocardial ischemia (MI) injury. SOCE, comprising STIM1, ORAI1 and TRPC1, contributesto intracellular Ca2þ ([Ca2þ]i) accumulation in cardiomyocytes. The purified extract of steamed Panaxginseng (EPG) attenuated [Ca2þ]i overload against MI injury. Thus, the aim of this study was to investigatethe possibility of EPG affecting p-caveolin-1 to further mediate SOCE/[Ca2þ]i against MI injury in neonatalrat cardiomyocytes and a rat model. Methods: PP2, an inhibitor of p-caveolin-1, was used. Cell viability, [Ca2þ]i concentration were analyzedin cardiomyocytes. In rats, myocardial infarct size, pathological damages, apoptosis and cardiac fibrosiswere evaluated, p-caveolin-1 and STIM1 were detected by immunofluorescence, and the levels ofcaveolin-1, STIM1, ORAI1 and TRPC1 were determined by RT-PCR and Western blot. And, release of LDH,cTnI and BNP was measured. Results: EPG, ginsenosides accounting for 57.96%, suppressed release of LDH, cTnI and BNP, and protectedcardiomyocytes by inhibiting Ca2þ influx. And, EPG significantly relieved myocardial infarct size, cardiacapoptosis, fibrosis, and ultrastructure abnormality. Moreover, EPG negatively regulated SOCE viaincreasing p-caveolin-1 protein, decreasing ORAI1 mRNA and protein levels of ORAI1, TRPC1 and STIM1. More importantly, inhibition of the p-caveolin-1 significantly suppressed all of the above cardioprotectionof EPG. Conclusions: Caveolin-1 phosphorylation is involved in the protective effects of EPG against MI injury viaincreasing p-caveolin-1 to negatively regulate SOCE/[Ca2þ]i.

Diagnostic value of Thyroglobulin Measurement with Fine-needle Aspiration Biopsy for Lymph Node Metastases in Patients with a History of Differentiated Thyroid Cancer

Zhang, Hai-Shan,Wang, Ren-Jie,Fu, Qing-Feng,Gao, Shi,Sun, Bu-Tong,Sun, Hui,Ma, Qing-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Purpose: The aim of this study was to evaluate the diagnostic value of FNA-Tg for detecting lymph node metastases in patients with a history of differentiated thyroid cancer (DTC). Materials and Methods: A total of 58 patients with DTC diagnosis and evidence of single or multiple suspicious cervical lymph nodes were assessed. All underwent total or near-total thyroidectomy with (35 cases) or without (23 cases) radioiodine (RAI) ablation, followed by thyroid stimulating hormone (TSH) suppression therapy. A total of 68 lymph nodes were examined by ultrasound-guided fine needle aspiration (US-FNA) for both cytological examination and FNA-Tg measurement. Serum Tg and anti-thyroglobulin antibody (TgAb) levels were also measured. Diagnostic performance including sensitivity, specificity, accuracy, positive (PPV) and negative predictive value (NPV) of FNAC and FNA-Tg were calculated and compared. The Spearman's rank correlation coefficient was used to estimate the relationship between FNA-Tg and serum TgAb. Results: The FNA-Tg levels were significantly higher with DTC metastatic lymph nodes (median 927.7 ng/mL, interquartile range 602.9 ng/mL) than non-metastatic lymph nodes (median 0.1 ng/mL, interquartile range 0.4 ng/mL) (p<0.01). Considering 1.0 ng/mL as a threshold value for FNA-Tg, the sensitivity, specificity, accuracy, PPV and NPV of FNA-Tg were 95.7%, 95.5%, 95.6%, 97.8% and 91.3%, respectively. The sensitivity and accuracy of the combination of FNAC and FNA-Tg were significantly higher than that of FNAC alone (p<0.05). The diagnostic performance of FNA-Tg was not significantly different between cases with or without RAI ablation, and the serum TgAb levels did not interfere with FNA-Tg measurements. Conclusions: Measurement of FNA-Tg is useful. The combination of FNAC and FNA-Tg is more sensitive and accurate for detecting lymph node metastases in patients with a history of DTC than FNAC alone. Serum TgAbs appear to be irrelevant for measurement of FNA-Tg.

A highly area-efficient controller for capacitive touch screen panel systems

Hwang, Tong-Hun,Cui, Wen-Hai,Yang, Ik-Seok,Kwon, Oh-Kyong IEEE 2010 IEEE transactions on consumer electronics Vol.56 No.2

<P>In this paper, a highly area-efficient controller for capacitive touch screen panels (TSPs) is proposed. The proposed controller uses a 10-bit successive approximation register analog-to-digital converter (SAR ADC) with an adder to compensate for the capacitance variation in the TSP and for the offset voltage variation in the charge amplifier of the sensing circuit. By using the proposed compensation method, the area of the controller can be reduced by 90.3% of the area of the conventional controllers. The measurement results showed that the signal-to-noise ratio (SNR) of the controller increases from 12.5 to 21.3 dB after compensation. Also, its spatial jitter decreases from ±1.5 to ±0.46 mm, which is 7% of the sensor pitch of 8 mm</P>

Antiviral Activities of Natural Compounds Derived from Traditional Chinese Medicines against Porcine Circovirus Type 2 (PCV2)

Na Sun,Tong Yu,Jun-Xing Zhao,Yaogui Sun,Junbing Jiang,Zhi-Bian Duan,WenkuiWang,Yuan-Liang Hu,Hai-Min Lei,HongquanLi 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

Twenty natural compounds isolated fromtraditional Chinese medicines were examined for theirantiviral activities against PCV2 in vitro. Antiviral activitywas analyzed by the assays of blocking of virus entry,inhibition of virus replication and inactivation of virusparticles in the virus suspension. 50% cytotoxic concentration(CC50) and 50% effective concentration (EC50) for eachcompound were determined by using the MTT method andimmunofluorescence assay (IFA), respectively. The resultsobtained from the inhibition of virus replication andinactivation of virus particles showed that the maximuminhibition rates for matrine and scutellarin were 57 and72.69% and their SI were 29.87 and 18.66, respectively. However, the results from the assays of inhibition of virusentry and inhibition of virus replication demonstrated thatboth aesculetin and arecoline hydrochloride stimulatedPCV2 replication, with the maximum stimulation rate of387.3 and 219.4%. In summary, both matrine and scutellarinhad antiviral activity against PCV2, while aesculetin andarecoline hydrochloride promoted PCV2 infection replicationin vitro.