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Fan, Fang-Tian,Shen, Cun-Si,Tao, Li,Tian, Chao,Liu, Zhao-Guo,Zhu, Zhi-Jie,Liu, Yu-Ping,Pei, Chang-Song,Wu, Hong-Yan,Zhang, Lei,Wang, Ai-Yun,Zheng, Shi-Zhong,Huang, Shi-Le,Lu, Yin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Pyruvate kinase isozyme type M2 (PKM2) was first found in hepatocellular carcinoma (HCC), and its expression has been thought to correlate with prognosis. A large number of studies have demonstrated that epithelial-mesenchymal transition (EMT) is a crucial event in hepatocellular carcinoma (HCC) and associated metastasis, resulting in enhanced malignancy of HCC. However, the roles of PKM2 in HCC EMT and metastasis remain largely unknown. The present study aimed to determine the effects of PKM2 in EGF-induced HCC EMT and elucidate the molecular mechanisms in vitro. Our results showed that EGF promoted EMT in HCC cell lines as evidenced by altered morphology, expression of EMT-associated markers, and enhanced invasion capacity. Furthermore, the present study also revealed that nuclear translocation of PKM2, which is regulated by the ERK pathway, regulated ${\beta}$-catenin-TCF/LEF-1 transcriptional activity and associated EMT in HCC cell lines. These discoveries provide evidence of novel roles of PKM2 in the progression of HCC and potential therapeutic target for advanced cases.
Association Between MDM2 SNP309 T>G and Risk of Gastric Cancer: A Meta-analysis
Tian, Xin,Tian, Ye,Ma, Ping,Sui, Cheng-Guang,Meng, Fan-Dong,Li, Yan,Fu, Li-Ye,Jiang, Tao,Wang, Yang,Ji, Fu-Jian,Fang, Xue-Dong,Jiang, You-Hong Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: As a negative regulator of P53, MDM2 plays an important role in carcinogenesis; a polymorphism in its promoter region. SNP309 T>G, is known to increase the expression of MDM2, thus being considered related to higher susceptibility to neoplasia. However, no agreement has been achieved regarding its effects on gastric cancer. Methods: The present systematic meta-analysis was performed based on comprehensive literature search from Pubmed, Web of science and CBM databases. Results: It was suggested from 6 independent studies that the GG genotype is associated with a significantly increased risk of gastric cancer (Recessive: OR = 1.43, 95% CI = 1.08-1.91, P = 0.013), and subgroup analysis also confirmed the relationship (English publications-recessive model: OR = 1.45, 95% CI = 1.10-1.91, P = 0.009; Studies in China-recessive model: OR = 1.58, 95% CI = 1.08-2.30, P = 0.017). No publication bias was detected. Conclusion: The meta-analysis indicated a significant inverse association between GG genotype carriage and elevated risk of gastric cancer. However, more studies and detailed information are needed to fully address the topic.
Orthogonal-Gradient Measurement Matrix Construction Algorithm
Pan, Tian,Choi, Youngjune,Tian, Shujuan,Fan, Xiaoping,Li, Zhetao,Sekiya, Hiroo Institution of Electrical Engineers 2016 Chinese journal of electronics Vol.25 No.1
<P>An orthogonal-gradient measurement matrix construction algorithm is proposed for reducing the maximum and average mutual-coherence of sensing matrix. It shrinks Gram matrix based on equiangular tight frame theory. An orthogonal-gradient factor matrix is deduced. It obtains an optimized measurement matrix with the orthogonal-gradient factor matrix. The results of experiments show that the proposed algorithm effectively reduces the maximum and average mutual-coherence of sensing matrix. This leads to a better reconstruction performance for signals with different sparsities compared with Gaussian matrix, Elad's, Xu's, Vahid's and Li's methods.</P>
Liver metabolic perturbations of heat-stressed lactating dairy cows
Fan, Caiyun,Su, Di,Tian, He,Li, Xiaojiao,Li, Yu,Ran, Lei,Hu, Ruiting,Cheng, Jianbo Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8
Objective: The objective of the present study was to elucidate the mechanism underlying liver metabolic perturbations in dairy cows exposed to heat stress (HS). Methods: Liquid chromatography massabl spectrometry was used to analyze metabolic differences in livers of 20 dairy cows, with and without exposure to HS. Results: The results revealed 33 potential metabolite candidate biomarkers for the detection of HS in dairy cows. Fifteen of these metabolites (glucose, lactate, pyruvate, acetoacetate, ${\beta}$-hydroxybutyrate, fumaric acid, citric acid, choline, glycine, proline, isoleucine, leucine, urea, creatinine, and orotic acid) were previously found to be potential biomarkers of HS in plasma or milk, discriminating dairy cows with and without HS. Conclusion: All the potential diagnostic biomarkers were involved in glycolysis, amino acid, ketone, tricarboxylic acid, or nucleotide metabolism, indicating that HS mainly affected energy and nucleotide metabolism in lactating dairy cows.
Exosomes from CIITA-Transfected CT26 Cells Enhance Anti-tumor Effects
Fan, Wen,Tian, Xing-De,Huang, E.,Zhang, Jia-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Aim: To study anti-tumor effects of exosomes from class II transactivator (CIITA) gene transfected CT26 cells. Methods: In this study, we established an MHC class II molecule-expressing murine colon cancer cell line (CT26-CIITA) by transduction of the CIITA gene. Immune effects in vitro and tumor protective results in vivo were tested and monitored. Results: Exosomes from CT26-CIITA cells were found to contain a high level of MHC class II protein. When loaded on dendritic cells (DCs), exosomes from CT26-CIITA cells significantly increased expression of MHC class II molecules, CD86 and CD80, as compared to exosomes from CT26 cells. In vitro assays using co-culture of immunized splenocytes and exosome-loaded DCs demonstrated that CIITA-Exo enhanced splenocyte proliferation and IFN-${\gamma}$ production of CD4+T cells, while inhibiting IL-10 secretion. In addition, compared to exosomes from CT26 cells, CT26-CIITA-derived exosomes induced higher TNF-${\alpha}$ and IL-12 mRNA levels. A mouse tumour preventive model showed that CT26-CIITA derived exosomes significantly inhibited tumour growth in a dose-dependent manner and significantly prolonged the survival time of tumour-bearing mice. Conclusion: Our findings indicate that CT26-CIITA-released exosomes are more efficient to induce anti-tumour immune responses, suggesting a potential role of MHC class II-containing tumour exosomes as cancer vaccine candidates.
Thermal and mechanical properties of poly(lactic acid)-based ternary composites
( Fan-long Jin ),( Qing-qing Pang ),( Tian-yi Zhang ),박수진 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Bio-based poly(lactic acid) (PLA) ternary composites were prepared by the solution blending of PLA, polybutylene succinate (PBS), and nano-CaCO3. The effects of PBS and nano-CaCO3 on the thermal stability,fracture toughness, mechanical properties, and morphology of PLA were investigated using several techniques. The morphology of the composites was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). As a result, the fracture toughness and flexural strength of PLA were improved by the introduction of PBS and further enhanced by the addition of nano-CaCO3.
Fan-Long Jin,Chun-Liu Ma,Bao-Tian Guo,박수진 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.10
The surface of graphene (GE) was modified with a silane coupling agent, and its surface characteristics were investigated. Epoxy resin/GE nanocomposites were prepared via solution blending. The effect of the GE content on the thermal stability, flexural properties, impact strength, and morphology of the nanocomposites was studied. The thermal stability of the epoxy resin increased with the addition of pristine GE and surface-modified GE. The flexural strength, elastic modulus, and impact strength of the nanocomposites with surface-modified GE increased significantly with increasing GE content. Scanning electron microscopy images of the nanocomposites with surface-modified GE show a ductile deformation morphology, which is consistent with their high impact strength.
Increasing the Productivity of TNFR-Fc in GS-CHO Cells at Reduced Culture Temperatures
Tian-Ci Kou,Li Fan,Yan Zhou,Zhao-Yang Ye,Liang Zhao,Wen-Song Tan 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.1
In an effort to improve TNFR-Fc production in GS-CHO cells, batch cultures were performed to investigate the effects of low culture temperature on TNFR-Fc production. It was observed that low culture temperatures resulted in cell cycle arrest in the G1 phase, led to suppressed cell growth, and prolonged the culture period. Although the highest TNFR-Fc concentration was achieved with a culture temperature of 32°C at 247.4 mg/L, the maximum q_(TNFR-Fc) of 15.7 pg/cells/day was achieved at 30°C. Because the inhibition effect on cell growth at 30°C compromised its beneficial effects specifically to TNFR-Fc productivity, TNFR-Fc concentration at this temperature was not significantly increased. Furthermore, the increase in productivity of specific TNFR-Fc at low culture temperatures was also found to be due to an increase in the transcriptional level of the TNFR-Fc gene, determined by RT-PCR analysis. In addition, low culture temperatures had no significant effect on the degree of sialylation of TNFRFc. Taken together; a biphasic cultivation process developed in a fed-batch mode with a low temperature-production phase enhanced TNFR-Fc production by GS-CHO cells and therefore offers major potential for bioprocess optimization.
Video-assisted Thoracoscopic Surgery for Treatment of Earlystage Non-small Cell Lung Cancer
Fan, Xing-Long,Liu, Yu-Xia,Tian, Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objectives: To evaluate the safety, efficacy, and invasiveness of lobectomy by video-assisted thoracoscopic surgery (VATS) in the treatment of stage I/II non-small cell lung cancer (NSCLC). Methods: A total of 148 patients presenting with Stage I or II NSCLC were enrolled into our study, comprising 71 who underwent VATS and 77 patients undergoing conventional thoracotomic lobectomy, in combination with systematic lymph node resection. Results: It was found that VATS was superior to conventional thoracotomy in terms of the duration of surgery, intraoperative blood loss, frequency of the need to administer postoperative analgesia, thoracic intubation indwelling time, post-operative hospital stay, and survival rate (P<0.05). We saw no obvious difference in the number of resected lymph nodes with either approach. Conclusions: VATS lobectomy is a safe and reliable surgical approach for the treatment of Stage I/II NSCLC, characterized by significantly minimal invasiveness, rapid post-operative recovery, and markedly lower loss of blood.