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Thakor Rashmi,Mistry Harsh,Bariya Himanshu 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.4
Fungi produce phytohormones like indole-3-acetic acid (IAA), which stimulates the growth of plants. In the present study, Aspergillus niger (AN) and Aspergillus caespitosus (DS-3) were identifi ed in screens for highly effi cient IAA-producing fungi. Initially, IAA production was assessed through UV–Visible spectroscopic analysis using Salkowski’s reagent. HPTLC confi rmed the presence of IAA in all fungal extracts when compared with the standard IAA. HPTLC analysis revealed that AN exhibit the highest IAA production, which was 251.86 ± 0.7 μg ml −1 . AN, DS-3 and a combination of both strains as a growth-promoting consortium (GPC-1) were applied to Trigonella foenum-graecum L seeds for 6 h. After 14 days, the fenugreek plants were used for both the physiological and biochemical analysis. Results indicated that the GPC-1 induced more desirable physiological properties, with higher protein content, carbohydrate content, total phenolic content and antioxidant activity compared to treatment with individual PGPFs or distilled water. The extract from fenugreek plants treated with GPC-1 had the maximum diosgenin content (342.374 ± 0.67 μg ml −1 ), as assessed by HPTLC.
LAL THAKORE B.B.,SNEH MATHUR,R.B. SHINGH,B.P. CHAKRAVARTI 한국응용곤충학회 1987 한국응용곤충학회지 Vol.26 No.4
토양에 첨가한 8종의 Oil cake 중 Margosa, Undi, Karanj cake은 생강뿌리썩음병의 발생을 억제하였다. 생강수량의 증진효과는 Karanj, Roselle, Mustard cake의 순으로 높았다. Out of 8 oil cakes used for soil amendment, Margosa, Undi and Karanj cakes were found to reduce the ginger rot. However, maximum increase in yield was found in Karanj cake followed by Roselle and Mustard cakes.
B.B. LAL THAKORE,SNEH MATHUR,R.B. SINGH 한국응용곤충학회 1987 한국응용곤충학회지 Vol.26 No.2
땅콩녹병과 검은 무늬병을 방제하기 위한 살균제를 선정하기 위하여 실내와 포장에서 실험한 결과 12개 공시약제중 Biloxazole이 상기 두 병해의 동시방제에 효과적이었으며 땅콩 수량도 증진되였다. Out of twelve fungicides evaluated in vitro and in vitro to manage rust and leaf spot diseases: Biloxazole was found to control both the diseases simultaneously and also increased the yield of peanut.
Yong-Hee Kim,Thakor, Nitish V.,Schieber, Marc H.,Hyoung-Nam Kim IEEE 2015 IEEE transactions on neural systems and rehabilita Vol.23 No.3
<P>Future generations of brain-machine interface (BMI) will require more dexterous motion control such as hand and finger movements. Since a population of neurons in the primary motor cortex (M1) area is correlated with finger movements, neural activities recorded in M1 area are used to reconstruct an intended finger movement. In a BMI system, decoding discrete finger movements from a large number of input neurons does not guarantee a higher decoding accuracy in spite of the increase in computational burden. Hence, we hypothesize that selecting neurons important for coding dexterous flexion/extension of finger movements would improve the BMI performance. In this paper, two metrics are presented to quantitatively measure the importance of each neuron based on Bayes risk minimization and deflection coefficient maximization in a statistical decision problem. Since motor cortical neurons are active with movements of several different fingers, the proposed method is more suitable for a discrete decoding of flexion-extension finger movements than the previous methods for decoding reaching movements. In particular, the proposed metrics yielded high decoding accuracies across all subjects and also in the case of including six combined two-finger movements. While our data acquisition and analysis was done off-line and post processing, our results point to the significance of highly coding neurons in improving BMI performance.</P>
Insulin enhances neurite extension and myelination of diabetic neuropathy neurons
( Vuong M. Pham ),( Nitish Thakor ) 대한통증학회 2022 The Korean Journal of Pain Vol.35 No.2
Background: The authors established an in vitro model of diabetic neuropathy based on the culture system of primary neurons and Schwann cells (SCs) to mimic similar symptoms observed in in vivo models of this complication, such as impaired neurite extension and impaired myelination. The model was then utilized to investigate the effects of insulin on enhancing neurite extension and myelination of diabetic neurons. Methods: SCs and primary neurons were cultured under conditions mimicking hyperglycemia prepared by adding glucose to the basal culture medium. In a single culture, the proliferation and maturation of SCs and the neurite extension of neurons were evaluated. In a co-culture, the percentage of myelination of diabetic neurons was investigated. Insulin at different concentrations was supplemented to culture media to examine its effects on neurite extension and myelination. Results: The cells showed similar symptoms observed in in vivo models of this complication. In a single culture, hyperglycemia attenuated the proliferation and maturation of SCs, induced apoptosis, and impaired neurite extension of both sensory and motor neurons. In a co-culture of SCs and neurons, the percentage of myelinated neurites in the hyperglycemia-treated group was significantly lower than that in the control group. This impaired neurite extension and myelination was reversed by the introduction of insulin to the hyperglycemic culture media. Conclusions: Insulin may be a potential candidate for improving diabetic neuropathy. Insulin can function as a neurotrophic factor to support both neurons and SCs. Further research is needed to discover the potential of insulin in improving diabetic neuropathy.
Soil Amendment wit Oil Cakes in Ginger Field for Rhizome Rot Control
랄타코레,마투르,차크라바르티,Lal Thakore B.B.,Mathur Sneh,Singh R.B.,Chakravarti B.P. Korean Society of Applied Entomology 1987 한국식물보호학회지 Vol.26 No.4
토양에 첨가한 8종의 Oil cake 중 Margosa, Undi, Karanj cake은 생강뿌리썩음병의 발생을 억제하였다. 생강수량의 증진효과는 Karanj, Roselle, Mustard cake의 순으로 높았다. Out of 8 oil cakes used for soil amendment, Margosa, Undi and Karanj cakes were found to reduce the ginger rot. However, maximum increase in yield was found in Karanj cake followed by Roselle and Mustard cakes.
Polymeric nanoparticles as dual-imaging probes for cancer management
Menon, Jyothi U.,Jadeja, Parth,Tambe, Pranjali,Thakore, Dheeraj,Zhang, Shanrong,Takahashi, Masaya,Xie, Zhiwei,Yang, Jian,Nguyen, Kytai T. Techno-Press 2016 Biomaterials and Biomechanics in Bioengineering Vol.3 No.3
This article reports the development of biodegradable photoluminescent polymer (BPLP)-based nanoparticles (NPs) incorporating either magnetic nanoparticles (BPLP-MNPs) or gadopentate dimeglumine (BPLP-Gd NPs), for cancer diagnosis and treatment. The aim of the study is to compare these nanoparticles in terms of their surface properties, fluorescence intensities, MR imaging capabilities, and in vitro characteristics to choose the most promising dual-imaging nanoprobe. Results indicate that BPLP-MNPs and BPLP-Gd NPs had a size of $195{\pm}43nm$ and $161{\pm}55nm$, respectively and showed good stability in DI water and 10% serum for 5 days. BPLP-Gd NPs showed similar fluorescence as the original BPLP materials under UV light, whereas BPLP-MNPs showed comparatively less fluorescence. VSM and MRI confirmed that the NPs retained their magnetic properties following encapsulation within BPLP. Further, in vitro studies using HPV-7 immortalized prostate epithelial cells and human dermal fibroblasts (HDFs) showed > 70% cell viability up to $100{\mu}g/ml$ NP concentration. Dose-dependent uptake of both types of NPs by PC3 and LNCaP prostate cancer cells was also observed. Thus, our results indicate that BPLP-Gd NPs would be more appropriate for use as a dual-imaging probe as the contrast agent does not mask the fluorescence of the polymer. Future studies would involve in vivo imaging following administration of BPLP-Gd NPs for biomedical applications including cancer detection.
薄層 크로마토그라피와 指標 곰팡이의 利用에 의한 밀에 있어서 Triadimefon의 잔류량 분석
KALPNA BHATNAGAR,B.B. LAL THAKORE,SNEH MATHUR,B.P. CHAKRAVARTI 한국응용곤충학회 1986 한국응용곤충학회지 Vol.25 No.4
Macrophomina phaseolina균(菌)을 이용(利用)하여 살균제 Triadimefon의 밀 품종 Kharchia와 Lal Bahadur에 있어서 수확후의 잔류량을 조사한 결과 살포식물의 밀짚에서는 Triadimefon이 검출되었으나 밀 종자에서는 검출되지 않았다. 잔류량 조사를 위해 개발된 박층(薄層) 크로마토그라피에 의하여도 동일한 결과를 얻었다. 기주체내에서 Triadimefon은 Triadimenol로 전환되었다. By using the test fungus Macrophomina phaseolina, residues of triadimefon were found in straw collected after harvest from sprayed plants of wheat varieties Kharchia and Lal Bahadur but grains contain no such residues. Thin layer chromatographic method was developed to detect residues of the fungicide which was found to be present in straw of sprayed plants of both the varieties. No residues could be detected in grain samples. It was found that triadimefon was converted in triadimenol in/to host.
Single-Finger Neural Basis Information-Based Neural Decoder for Multi-Finger Movements
Choi, Hwayoung,You, Kyung-Jin,Thakor, Nitish V.,Schieber, Marc H.,Shin, Hyun-Chool IEEE 2018 IEEE transactions on neural systems and rehabilita Vol.26 No.12
<P>In this paper, we investigate the relationship between single and multi-finger movements. By exploiting the neural correlation between the temporal firing patterns between movements, we show that the Pearson’s correlation coefficient for the physically related movement pairs are greater than those of others; the firing rates of the neurons that are tuned to a single-finger movements also increases when the corresponding multi-finger movements are instructed. We also use a hierarchical cluster analysis to verify not only the relationship between the single and multi-finger movements, but also the relationship between the flexion and extension movements. Furthermore, we propose a novel decoding method of modeling neural firing patterns while omitting the training process of the multi-finger movements. For the decoding, the Skellam and Gaussian probability distributions are used as mathematical models. The probabilistic distribution model of the multi-finger movements was estimated using the neural activity that was acquired during single-finger movements. As a result, the proposed neural decoding accuracy comparable with that of the supervised neural decoding accuracy when all of the neurons were used for the multi-finger movements. These results suggest that only the neural activities of single-finger movements can be exploited for the control of dexterous multi-finger neuroprosthetics.</P>