Prognosis of Eight Chinese Cases of Primary Vaginal Yolk Sac Tumor with a Review of the Literature

Tang, Qiong-Lan,Jiang, Xue-Feng,Yuan, Xiao-Ping,Liu, Yong,Zhang, Lin,Tang, Xiao-Feng,Zhou, Jia-Jia,Li, Hai-Gang,Fang, Jian-Pei,Xue, Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Primary vaginal yolk sac tumor is a rare malignancy in the pediatric population, and a diagnostic challenge and appropriate initial treatment remains unsolved. The aim of this study was to investigate the clinicopathologic features, treatment and prognosis of this tumor. Materials and Methods: Eight cases of primary vaginal yolk sac tumor were reported with a literature review. Results: There were 4 pure yolk sac tumor cases and four mixed germ cell tumors containing yolk sac tumor element, including two cases with embryonal carcinoma and two cases with embryonal carcinoma and dysgerminoma. Partial vaginectomy was performed in four cases and all patients received chemotherapy. 85 cases in literatures were reviewed and 9 cases were misdiagnosed. Follow-up data was available in 77 cases and 5-year overall survival rate was 87.6%. 5-year survival rate of biopsy with chemotherapy, conservative surgery with chemotherapy and radical surgery with chemotherapy was 91.1%, 100% and 28.6%, respectively (p<0.001). Compared to cases without relapse or metastasis after initial treatment, patients with relapse or metastasis had a shorter overall survival (35.6% vs 96.6%, p<0.001). Conclusions: Mixed germ cell tumor containing yolk sac tumor element was not uncommon and partial vaginectomy may be a good choice for primary vaginal mixed yolk sac tumor type to eradicate local tumor cells and provide complete information for pathological diagnosis and postoperative adjuvant therapy.

Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Tang, Jin-Feng,Yu, Zhong-Hua,Liu, Tie,Lin, Zi-Ying,Wang, Ya-Hong,Yang, La-Wei,He, Hui-Juan,Cao, Jun,Huang, Hai-Li,Liu, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.

Golgi Phosphoprotein 2 Down-regulates the Th1 Response in Human Gastric Cancer Cells by Suppressing IL-12A

Tang, Qing-Feng,Ji, Qing,Tang, Yu,Hu, Song-Jiao,Bao, Yi-Jie,Peng, Wen,Yin, Pei-Hao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Golgi phosphoprotein 2 (GOLPH2) is a very important biomarker in a variety of diseases. Its biological function is not clear, particularly in gastric cancer. To investigate the role of GOLPH2 in human gastric cancer, and determine its effect on the Th1 lymphocyte response, its expression and that of IL-12A were measured by real-time PCR and immunohistochemistry. The relationship between GOLPH2 and IL-12A was analysed statistically. The effect of GOLPH2 on the Th1 lymphocyte response was investigated with an in vitro co-culture system. The results showed that in human gastric cancer, the expression of GOLPH2 was significantly higher and the expression of IL-12A was lower than in normal gastric mucosal tissues, and the expression levels of GOLPH2 and IL-12A were negatively correlated. In addition, obvious down-regulation of the Th1 response was observed when lymphocytes were co-cultured with gastric cancer SGC7901 cells over-expressing GOLPH2. GOLPH2 down-regulated the expression of IL-12A, and inhibited the expression of TNF-${\alpha}$ and IFN-${\gamma}$. The results indicated that GOLPH2 down-regulates the Th1 response via suppression of IL-12A in human gastric cancer, and this might provide a target for the prevention and treatment.

ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis

Tang Ri,Tang Ri,Xu Qiaoyi,Feng Jinhua,Zhou Yang,Xing Shunpeng,He Zhengyu,Gao Yuan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Recent clinical research has revealed that mechanical ventilation (MV) can initiate pulmonary fibrosis and induce mechanical ventilation-induced pulmonary fibrosis (MVPF). However, the underlying mechanism remains largely uncharacterized. Based on a mouse model of MVPF and an alveolar epithelial cell cyclic strain model, the present study explores the possible mechanism of MVPF. Single-cell RNA-sequencing and EV RNA-sequencing analysis revealed that MV promoted apoptosis signal-regulating kinase 1 (ASK1)-mediated endoplasmic reticulum (ER) stress pathway activation and extracellular vesicle (EV) release from alveolar epithelial cells. Furthermore, the ASK1-ER stress pathway was shown to mediate mechanical stretch (MS)- or MV-induced EV release and lung fibroblast activation in vivo and in vitro. These processes were suppressed by ER stress inhibitors or by silencing ASK1 with ASK1- short hairpin RNA (shRNA). In addition, MVPF was suppressed by inhibiting ASK1 and ER stress in vivo. Therefore, the present study demonstrates that ASK1-ER stress pathway-mediated fibrotic-EV release from alveolar epithelial cells contributes to fibroblast activation and the initiation of pulmonary fibrosis during MV. The inhibited release of EVs targeting the ASK1-ER stress pathway might be a promising treatment strategy for MVPF.

Population Differentiation of Procypris rabaudi (Tchang) in the Upper Reaches of the Yangtze River Revealed by Mitochondrial DNA and RAPD Markers

Yong Feng He,Qiong Ying Tang,Jian Wei Wang,Huan Zhang Liu,De Qing Tan 한국유전학회 2008 Genes & Genomics Vol.30 No.3

Procypris rabaudi (Tchang) is a cyprinid fish endemic to middle and upper reaches of the Yangtze River. Besides in main stream and large tributaries, there exists an early matured, small-sized ecological type in a small tributary, Tang River. In this study, mitochondrial DNA cytochrome b (cyt b) gene sequence analysis and randomly amplified polymorphic DNA (RAPD) analysis were performed to investigate the differentiation of the Tang River population from the Mudong reach population of the Yangtze River, with the purpose of conservation and exploitation of this fish. In the 1140 bps of cyt b gene sequence surveyed, 20 sites were found polymorphic, which defined 23 haplotypes. Among them, four haplotypes accounted for 54.4% of all individuals, while population-specific haplotypes occurred in low frequencies. Analysis of molecular variation on cyt b data revealed no significant partition existing between Tang River population and Mudong reach population. Analyses of 132 RAPD loci suggested that genetic variation between populations was significant, though values of different FST were not very high. The results revealed low genetic diversity and the beginning of population differentiation, suggesting that Tang River population should be designated as a separate Management Unit.

Generalized Analysis of Message Propagation on Social Network

Min-Feng Wang,Chi-Sheng Huang,Meng-Feng Tsai,Bo-Ru Song,Shin-Fu Su,Cheng-Hsien Tang 보안공학연구지원센터 2012 International Journal of Future Generation Communi Vol.5 No.2

IOMMU Para-Virtualization for Efficient and Secure DMA in Virtual Machines

Tang, Hongwei,Li, Qiang,Feng, Shengzhong,Zhao, Xiaofang,Jin, Yan Korean Society for Internet Information 2016 KSII Transactions on Internet and Information Syst Vol.10 No.12

IOMMU is a hardware unit that is indispensable for DMA. Besides address translation and remapping, it also provides I/O virtual address space isolation among devices and memory access control on DMA transactions. However, currently commodity virtualization platforms lack of IOMMU virtualization, so that the virtual machines are vulnerable to DMA security threats. Previous works focus only on DMA security problem of directly assigned devices. Moreover, these solutions either introduce significant overhead or require modifications on the guest OS to optimize performance, and none can achieve high I/O efficiency and good compatibility with the guest OS simultaneously, which are both necessary for production environments. However, for simulated virtual devices the DMA security problem also exists, and previous works cannot solve this problem. The reason behind that is IOMMU circuits on the host do not work for this kind of devices as DMA operations of which are simulated by memory copy of CPU. Motivated by the above observations, we propose an IOMMU para-virtualization solution called PVIOMMU, which provides general functionalities especially DMA security guarantees for both directly assigned devices and simulated devices. The prototype of PVIOMMU is implemented in Qemu/KVM based on the virtio framework and can be dynamically loaded into guest kernel as a module, As a result, modifying and rebuilding guest kernel are not required. In addition, the device model of Qemu is revised to implement DMA access control by separating the device simulator from the address space of the guest virtual machine. Experimental evaluations on three kinds of network devices including Intel I210 (1Gbps), simulated E1000 (1Gbps) and IB ConnectX-3 (40Gbps) show that, PVIOMMU introduces little overhead on DMA transactions, and in general the network I/O performance is close to that in the native KVM implementation without IOMMU virtualization.

High-Purity Carbon Dots Prepared by Modulating Morphology of Carbon Nano-Crystals: In Vitro and In Vivo Multi-Color Bioimaging

Feng Huo,Yuran Tang,Yuhang Liu,Chao Tan,Lin Chang,Zakaria Mirza,Wei Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.15 No.01

Due to the unique optical properties and excellent biological applications, carbon-based luminescent materials have become a research hotspot in recent years. In this paper, we report a synthesis method under mild condition, in which Carbon NanoCrystals (CNCs) were first prepared, and then high-purity multi-color carbon dots (HPMCCDs) were synthesized by modulating crystal morphology. The prepared nonfluorescent CNCs are, respectively, dissolved in a strong polar solvent and a weak polar solvent to prepare HPMCCDs. It exhibits the multi-excitation and multi-emission features (from green to yellow). The high-purity carbon dots (HPCDs) prepared by using this method demonstrate the higher quantum yields (QYs) (53.7%) than that of the carbon dots (CD) by traditional hydrothermal methods with dialysis, and make full use of the atomic economic model. Besides, it shows the unique up-conversion luminescence property (UCLP) at lower wavelength excitation and, which can also be placed for a long time at room temperature without being oxidized or deteriorated. Furthermore, we explored the regulation of polar-chromism mechanism of the CNCs. Finally, the HPMCCDs can be used for multi-color bioimaging in vivo and in vitro and as well as many other potential applications.

Comparative Evaluation of an Error Checking Approach for MPSoC

Tang Liu,Huang-Zhang Qin,Hou-Yi Bin,Fang-Feng Cai,Zhang-Hui Bing 보안공학연구지원센터 2013 International Journal of Multimedia and Ubiquitous Vol.8 No.5

FORMATION OF REVERSED MICELLE NANORING BY A DESIGNED SURFACTANT-LIKE PEPTIDE

FENG QIU,YONGZHU CHEN,CHENGKANG TANG,YANRONG LU,XIAOJUN ZHAO,JINGQIU CHENG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.4

Designing self-assembling peptides as nanomaterials has been an attractive strategy in recent years, however, these peptides were usually studied in aqueous solutions for their self-assembling behaviors and applications. In this study, we have designed a surfactant-like peptide AGD with a wedge-like shape and studied its self-assembling behaviors in aqueous solution or nonpolar system. By analyzing the intermolecular hydrogen bond using FT-IR and characterizing the nanostructures with DLS, AFM and TEM, it was con¯rmed that AGD could not undergo selfassembly in aqueous solution while could self-assemble into well-ordered nanorings in nonpolar system. A molecular model has been proposed to explain how the nanorings were formed in the manner of reversed micelle. These results suggested a novel strategy to fabricate self-assembling peptide nanomaterials in nonpolar system, which could have potential applications in many ¯elds.