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Li, Taihua,Jeon, Ki-Seok,Suh, Yung Doug,Kim, Min-Gon Royal Society of Chemistry 2011 Chemical communications Vol.47 No.32
<P>A label-free, direct and noncompetitive homogeneous immunoassay, in which ochratoxin A (OTA) coupled with the anti-OTA antibody participates in fluorescence resonance energy transfer (FRET), was developed for the detection of OTA with great specificity and a detection limit of 1 ng mL<SUP>−1</SUP>.</P> <P>Graphic Abstract</P><P>A label-free, direct and noncompetitive homogeneous immunoassay, in which ochratoxin A (OTA) coupled with the anti-OTA antibody participates in fluorescence resonance energy transfer (FRET), was developed for the detection of OTA with great specificity and a detection limit of 1 ng mL<SUP>−1</SUP>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc12604k'> </P>
Pyrrolo-dC based fluorescent aptasensors for the molecular recognition of targets
Li, Taihua,Fu, Rongzhan,Park, Hyun Gyu Royal Society of Chemistry 2010 Chemical communications Vol.46 No.19
<P>Novel signal-on fluorescent aptasensors based on unlabeled aptamers and pyrrolo-dC have been developed for the detection of several targets with great specificity and sensitivity.</P> <P>Graphic Abstract</P><P>Novel signal-on fluorescent aptasensors based on unlabeled aptamers and pyrrolo-dC have been developed for the detection of several targets (ATP, thrombin, and PDGF) with great specificity and sensitivity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b923462d'> </P>
Li, Taihua,Jo, Eun-Jung,Kim, Min-Gon The Royal Society of Chemistry 2012 Chemical communications Vol.48 No.17
<P>A label-free fluorescence immunoassay system relying on fluorescence from the dianionic form of ochratoxin A (OTA) in the OTA/anti-OTA complex was developed. With an optimized system, the fluorescence immunoassay can be used to detect OTA in a highly specific manner with a detection limit of 0.5 ng mL<SUP>−1</SUP>.</P> <P>Graphic Abstract</P><P>A label-free fluorescence immunoassay system relying on fluorescence from the dianionic form of ochratoxin A (OTA) in the OTA/anti-OTA complex was developed. With an optimized system, the fluorescence immunoassay can be used to detect OTA in a highly specific manner with a detection limit of 0.5 ng mL<SUP>−1</SUP>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cc17088d'> </P>
Girigoswami, Agnishwar,Li, Taihua,Jung, Cheulhee,Mun, Hyo Young,Park, Hyun Gyu American Scientific Publishers 2009 Journal of nanoscience and nanotechnology Vol.9 No.2
<P>Here we describe a label-free detection strategy for point mutation in breast cancer susceptibility gene BRCA1 utilizing ligation chain reaction (LCR) and zip-code array. We amplified the genomic regions containing mutation sites by polymerase chain reaction (PCR) to prepare the template for LCR. Then we ligated a primary probe extended by zip-code complementary at the 5' end with a 3'-thiol modified secondary probe. The resulting ligated product was labeled with gold nanoparticles and allowed to hybridize with corresponding zip-code sequences on the microarray. Finally, we applied silver enhancement to amplify the signals. Using this strategy, we successfully genotyped BRCA1 mutation, which was detected by naked eye.</P>