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Taichi Usui,Takeomi Murata 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.5
Our purpose is to develop an efficient synthetic method of obtaining oligosaccharide unit insufficient amounts to study functions of glycans. Many exoglycosidases have been used as tools for the oligosaccharide synthesis. In contrast, a limited number of reports are available on the utilization of endoglycosidases. We describe herewith the efficient synthetic method of useful oligosaccharides and derivatives as biomaterials utilizing lysozyme, cellulase, and lacto-N-biosidase-mediated transglycosylations.
Detection of Grasping Position from Video Images Based on SSD
Taichi Kitayama,Huimin Lu,Yujie Li,Hyoungseop Kim 제어로봇시스템학회 2018 제어로봇시스템학회 국제학술대회 논문집 Vol.2018 No.10
Recently, consistent container transportation of roads and ships is mainstream of international freight transport. Because of various factors, automation of cargo handling work is required at the container terminal. Various causes are decrement of future labor force population by an increasing trend of container moving amount and declining birthrate and aging population. Therefore, this study presents the relative position of hanger and container measurement technology using Single Shot Multibox Detector (SSD) for the purpose of improvement of cargo handling work efficiency and unmanned container terminal. In the case of undetected by SSD, it will be detected using AKAZE feature. The proposed method is applied to 368 images of container gripping taken by a camera installed in a container crane. As a result, Interaction of Union (IoU) targeted for container gripping is 87.79%, and a detection rate is 94.57%.
Experimental validation of steering torque feedback simulator through vehicle running test
Taichi Shiiba,Wataru Murata 대한기계학회 2009 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.23 No.4
This paper describes a force feedback system based on real-time multibody dynamic analysis. This system can provide the analyzed reactive force to the operator through the operational device. In this study, this system is used as a steering torque feedback simulator of an automobile. This simulator can provide the haptic sensation of the steering wheel to the operator. For the purpose of evaluating the validity of the developed simulator, we conducted some vehicle running tests with an experimental electric vehicle. The results of these tests were compared with the results simulated on the steering torque feedback simulator. It was shown that the developed simulator can provide a suitable steering torque to the operator.
Taichi Chen,Hongzhi Xia,Shixiu Cui,Xueqin Lv,Xueliang Li,Yanfeng Liu,Jianghua Li,Guocheng Du,Long Liu 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.5
Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK- 7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future.