세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

Glycosylated Hemoglobin 分析에서의 Filter Separator를 利用한 比色法과 Ion Exchange Column法에 關한 比較硏究

金厚澤 상명대학교 논문집 1983 상명대학교논문집 Vol.11 No.-

Glycosylated Hemoglobin Assay was studied through the use of column method and the colorimetric method using the filter separator (Batch method). In this study, the two methods sere compared regarding the operation, condition and correlation of the assay. The results are as follows 1. The Batch method is more easy to operate than the ion exchange column method. 2. The ion exchange column method is dependent upon pH and temperature absolutely but the Batch method is not. 3. The analyses using the Batch method is shorter than the ion exchange method. 4. The correlation coefficient between the two methods is 0.967, the F value is 1.16,and the T value is 0.68. Consequently, the two methods are not significantly different from each other.

소변내의 17 Ketosteroid 분석에 있어서의 추출분리법에 관한 연구

金厚澤 건국대학교 1985 論文集 Vol.20 No.1

In urinary 17 ketosteroid analysis, we have studied about the extraction with ethyl ether and methylene chloride, and separation with chromatographic column. The results are as follows. The correlation between the extraction with ethyl ether and methylene chloride, r is 0.9748. The correlation between the extraction with methylene chloride and the chromatographic separation, r is 0.9811. Coefficient variation of the method using ethyl ether and methylene chloride and chromatographic column separation is 8.3%, 9.1%, 7.1% respectively. The extraction method takes a long tinge to dry but the evapourating time in column separation is not necessary. Reproducibility of chromatographic separation method is best in the above-three methods.

TiNi/Al6061 형상기억복합재료의 고온에서의 손상거동에 대한 AE 특성

이진경,박영철,구후택 한국비파괴검사학회 2002 한국비파괴검사학회지 Vol.22 No.1

금속복합재료(MMC) 내부에서의 강화재와 기지재사이의 열팽창계수의 차이에 의해 발생하는 인장잔류응력은 복합재료의 인장강도를 저하시키는 심각한 문제중의 하나이다. 따라서 본 연구에서는 TjNi/Al6061 형상기억복합재료내부의 인장잔류응력의 문제를 해결하기 위하여 강화재로써 TiNi 합금을 사용하였다. TiNi 합금은 형상기억효과를 이용하여 복합재료내부에 압축잔류응력을 발생시킴으로써 인장강도를 증가시키는 역할을 한다. 복합재료내부에 압축잔류응력을 발생시키기 위하여 예변형(pre-strain)을 가하였다. 본 연구에서는 예변형의 변화에 따른 압축잔류응력의 영향을 평가하였으며 AE 기법을 이용하여 고온에서의 복합재료의 미시적 손상거동과 예변형의 영향을 평가하였다. It has been known that tensile residual stresses occurring by the thermal expansion coefficient mismatch between fiber and matrix is a cause of the weak strength of metal matrix composites(MMCs). In order to solve this problem, TiNi alloy fiber was used as a reinforced material in TiNi/Al6061 shape memory alloy composite in this study. TiNi alloy fiber improves the tensile strength of the composite by causing compressive residual stress in matrix on the basis of its shape memory effect. Pre-strain was imposed to generate the compressive residual stresses inside the TiNi/Al6061 shape memory alloy composites. AE technique was used to quantify the microscopic damage behavior of the composite at high temperature. The effect of applied pre-strains on the AE behavior was also evaluated.

자궁내막증 병변에서의 Cytokine mRNA의 발현 양상

이택후(Taek Hoo Lee),김광수(Gwang Soo Kim),김일규(Il Gyu Kim),전상식(Sang Sik Chun),조영래(Young Lae Cho) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.9

목적: 자궁내막증은 최근에 그 빈도가 급격하게 증가되고 있으나 아직까지 병인과 치료법이 확실하게 정립되지 못하고 있는 부인과 내분비의 대표적인 질환이다. 최근 들어서 자궁내막증 환자의 불임의 원인으로 cytokine이 밀접하게 관여됨이 밝혀지고 있고 자궁내막증의 병인으로 복강내로 역류된 월경혈에 의한 복강내의 국소적 염증에 대한 개체의 반응정도와 면역체계의 변화가 중요하게 인정되고 있으나 아직까지 정확한 기전은 밝혀져 있지 않기 때문에 이에 저자는 자궁내막증 환자의 복수 내에서의 cytokine의 정량측정과 골반내 자궁내막증 병변조직에서의 cytokine mRNA의 발현 양상을 조사하여 자궁내막증 환자의 병인분석을 시도하였다. 연구방법: 자궁내막증 진단을 받고 복강경 혹은 개복 수술을 받은 30명의 환자에서 총 60개의 자궁내막증 병변이라고 의심되는 조직을 채취하여 이를 RT-PCR법을 이용하여 Cytokine 유전자의 발현양상을 조사하였다. 또한 7례의 정상대조군과 23례의 자궁내막증 환자의 복수를 ELISA법을 이용하여 정량분석을 시도하였다. 결과: 자궁내막증 환자의 복수내에서 IL-6와 IL-10의 농도는 자궁내막증의 임상적 중증도에 따라서 의미있게 증가되어 있었으나 IFN-γ, TNF-α, IL-1β, 그리고 IL-5의 농도는 정상 대조군에 비해서 변화가 없었다. 모든 예의 심부 자궁내막증 병변조직과 표재성 자궁내막증 병변조직에서 IL-1β cytokine mRNA의 발현을 볼 수 있었다. IL-5와 IL-6은 표재성 병변 12개 중에서 각각 2개의 black lesion에서만 발현을 볼 수 있었으며 IL-10은 표재성 병변에서는 12개중 2개에서 그리고 심부성 병변에서는 8개중 1개의 조직에서만 발현되었다. IFN-γ는 표재성 병변에서는 전혀 발현이 없었으나 심부성 병변에서는 8개중 4개의 조직에서 발현이 되었으며 TNF-α는 표재성 병변에서는 red 및 black 병변에서 각각 1개의 조직에서만 발현이 되었으나 심부성 병변에서는 역시 8개중 4개의 조직에서 발현이 되었다. 결론: 표재성 병변이 골반강내에 착상하여 염증성 반응이 일어날 수 있는 원인이 제공되면 IL-1β 혹은 TNF-α같은 염증성 cytokine이 분비가 되며 이로 인해 생성되는 단핵세포의 chemotactic factor에 의해 대식세포의 증가와 활성화가 이루어지고 이어서 복강내에 IL-6 등의 cytokine이 증가되며 마지막으로 여러 가지 증가된 cytokine에 대한 반대 반응으로 IL-10이 증가됨을 추정할 수가 있겠으며 이러한 가정은 앞으로 cytokine을 이용한 치료적 응용의 기초적인 연구로서 중요한 의미를 제공할 수 있다고 하겠다. Objective: The pathogenesis of endometriosis is generally accepted that retrograde menstruation and alterations in the local pelvic immune environment. This study was performed to help elucidate what kind of role various cytokines might play in the pathogenesis of endometriosis. Method: Concentrations of peritoneal fluid cytokines were compared in 7 women with normal pelvic finding and 23 women with endometriosis by enzyme-linked immunosorbent assay(ELISA). The patterns of cytokine mRNA expression in 8 ovarian endometrioma and 12 superficial pelvic endometriosis lesions were investigated by reverse transcription-polymerase chain reaction(RT-PCR) amplification method. Result: Both IL-6 and IL-10 levels in peritoneal fluid specimens with endometriosis tended to be higher than normal. However, there were no significant differences between peritoneal fluid concentrations of IFN-γ, TNF-α, IL-1β, and IL-5 of women with and without endometriosis. The levels of IL-6 and IL-10 were significantly higher in peritoneal fluid of women with severe endometriosis compared to women with mild endometriosis. IL-1β mRNA was expressed in all of 8 deep and 12 superficial endometriosis lesions. IL-5 and IL-6 mRNA were expressed in only two black lesions respectively, however, both were not expressed in the all deep lesions. Expressions of IL-10 mRNA occurred in one red and one black lesion while this was expressed in only one of the deep lesions. TNF-α mRNA was expressed in one red and one black lesion of 12 superficial lesions compared with four of the deep lesions. There was the difference between kinds of increased cytokines in the peritoneal fluid and those of expressed cytokines in the endometriotic lesions of patients with endometriosis. Conclusion: This study supports the concept that local immunologic factors may be important in the pathogenesis and pathophysiology of endometriosis. The pattern of cytokine mRNA expression of endometriotic lesions would seem to indicate that proinflammatory cytokines such as IL-1β and TNF-α are responsible for the development or progression of endometriosis.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후 ( Taek Hoo Lee ),김항진 ( Hang Jin Kim ),송건호 ( Gun Ho Song ),김대근 ( Dae Geun Kim ),전상식 ( Sang Sik Chun ),박윤규 ( Yoon Kyu Park ),서태광 ( Tae Kwang Suh ),전병균 ( Byeong Gyun Jeon ),류은경 ( Eun Kyung Ryu ),이은숙 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12

Immobilization of spermatozoa prior to intracytoplasmic sperm injection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization, cleavage, percentage of grade 1 embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes were inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30 μ I drop of mHTF supplemented with 10 % heat-inactivated follicular fluid(FF) at 37℃. On day 2, embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF+10 % FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to each treatment and ICSI was performed(88 head-first, 76 tail-first). The rates of normal fertilization were 81.8 % and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9 % and 68.8 %, 93.1 % and 74.1 % for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4 %(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

흡착제(吸着劑)의 피복(被覆)이 담배의 끽미(喫味)와 물리화학적(物理化學的) 성질 (性質)에 미치는 효과(效果)에 관한 연구(硏究)

박택규 ( Taek Kyu Park ),김기환 ( Ki Hwan Kim ),김후택 ( Hoo Taek Kim ) 건국대학교 기초과학연구소 1980 理學論集 Vol.6 No.-

The adsorbents of a large specific surface area was coated to shredded filler tobacco (Hicks tobacco grades 5; Nicotiana tabacum L.) for increased combustibility and reduced tar, nicotine in smoke. The adsorbents used are activated porous charcoal of a coconut-shell, wood charcoal, clinoptilolite and tobacco powder. Comparison of smoke composition from control cigarette and adsorbents coated cigarette were as follows; The combustibility was more increased in the case of more fireable, larger specific surface area, and larger porous adsorbent. Specially activated porous charcoal of a coconut-shell coated cigarette was most reduced contents of tar, nicotine, phenols and NOx in smoke.

표면 개질화된 천연제올라이트의 수분 흡착 특성

이후주(Hoo Joo Lee),이후근(Hoo Kun Lee),박근일(Geun Il Park),노성기(Seung Gy Ro),최호석(Ho Suk Choi),이용택(Yong Taek Lee) 大韓環境工學會 1999 대한환경공학회지 Vol.21 No.4

축산물 가격변동의 물가 파급영향에 대한 소비자 인식 분석

서상택 ( Sang Taek Seo ),김태후 ( Taek Hoo Kim ),김성섭 ( Seong Sup Kim ) 한국농업경제학회 2012 農業經濟硏究 Vol.53 No.1

This study analyzes the consumer`s recognition for the impact of fresh meats on general price level, which becomes an important issue to consumers who have frequently experienced animal diseases and price fluctuation of fresh meats in these days. 550 urban housewives were surveyed for the items with the number of purchase, the type of purchase, and the amount of expenditure in fresh meats in comparison with public service fees including demographical characteristics. Bardley-Terry model and logistic regression model were adopted to analyze the impacts and factors that price changes in fresh meats have on general price level. Results show that public service fees have more impacts on general price level than fresh meats. This implies that a relative compariosn of the impacts of price change in fresh meats and public service fees may lower the misunderstanding that fresh meats are main source of price fluctuation of general price.

Global nematicides market trend and R&D strategies of leading companies

Taek Su Shin,Jae Sung Im,Min Seop Cho,Hyun Hoo Park 한국응용곤충학회 2022 한국응용곤충학회 학술대회논문집 Vol.2022 No.04