품종에 따른 수수 메탄올 추출물 및 용매분획물의 항산화활성

우관식, 김정인, 고지연, 이재생, 송석보, 정태욱, 김기영, 곽도연, 오인석, 정헌상 忠北大學校 農業科學硏究所 2013 農業科學硏究 Vol.29 No.1

The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of sorghum according to varieties. The cultivated varieties were Hwanggeumchal-susu (HS), Milo-susu (MS) and Jungmo 4001 (JM4). The yield of methanolic extract of HS was 4.37%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 8.82, 2.46, 5.24, 24.49, and 47.23%, respectively. The highest total polyphenol, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 30.33 mg GAE/g ME, 4.69 mg CE/g ME, 25.86 mg TAE/g ME, and 675.09 μg CE/g ME from HS, respectively. The highest total polyphenol and flavonoid contents of solvent fractions (SF) were 52.87 mg GAE/g SF and 18.86 mg CE/ g SF in ethyl acetate fraction of HS, respectively. The highest total tannin and proanthocyanidin contents of solvent fractions were 39.27 mg TAE/g SF and 1210.74 μg CE /g SF in the butanol fraction of HS, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 20.54 and 33.85 mg TE/g SF in ethyl acetate fraction of HS, respectively. The highest catalase activity of the solvent fractions was 67.92 nmol/min/mL in ethyl acetate fraction of HS. The superoxide dismutase- like activities of methanolic extracts from HS, MS and WS were 20.22, 20.00 and 3.14 unit/mL, respectively. The results of this study show that notable antioxidant activities in sorghum flowers have significant health benefits.

21가지 장미꽃 품종의 페놀성 화합물 함량 및 항산화 활성

조연재(Yeon Jae Jo),서정현(Jeong Hyun Seo),홍채영(Chae Young Hong),김성태(Seung Tae Kim),최은경(Ehn-Kyoung Choi),김윤배(Yun-Bae Kim),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.4

본 연구는 21품종의 장미꽃에 대한 식품 및 화장품 원료로 사용 가능성을 살펴보기 위해 페놀성 화합물과 항산화 활성을 평가하였다. 총 폴리페놀, 총 플라보노이드 및 타닌 함량은 러버샤이 품종에서 각각 379.70 mg GAE/extract g, 50.10 mg CE/extract g 및 339.44 mg TAE/extract g으로 높게 나타났으며, 칠백리 품종에서 각각 107.25 mg GAE/extract g, 9.46 mg CE/extract g 및 36.31 mg TAE/extract g으로 낮았다. 페놀산 함량은 총 폴리페놀 함량과 유사한 결과를 보였으며, 러버샤이 품종에서 27.40 mg/extract g으로 높았다. 안토시아닌은 대부분 cyanidin-3,5-diglucoside로 확인되었으며 총 안토시아닌 함량은 유니나 품종에서 8.80 mg/extract g으로 가장 높았다. ABTS 및 DPPH 라디칼 소거 활성은 각각 428.10 및 419.59 mg AAE/extract g로 러버샤이 품종에서 가장 높았으며, 항산화 활성과 페놀 화합물의 함량과 높은 양의 상관관계를 보였다. 이러한 결과는 러버샤이와 같은 항산화 활성이 뛰어난 품종들이 관상 목적뿐만 아니라 높은 기능성을 가진 식품 및 화장품의 원료로 사용 가능성이 있다고 판단된다. This study evaluated the functional components and antioxidant activities of 21 different rose flower extracts, to confirm their value as a food and cosmetics ingredient. The highest values of total polyphenol, flavonoid and tannin contents (379.70±1.64 mg GAE/extract g, 50.10±0.76 mg CE/extract g, and 339.44±1.20 mg TAE/extract g, respectively) were obtained in Lover Shy. The proanthocyanidin content ranged from 61.88 mg CE/extract g in the Unina cultivar to 5.34±0.13 mg CE/extract g in the Aileen cultivar. The phenolic acid content obtained was highest in the Lover Shy cultivar (27.40±0.26 mg/extract g), whereas highest levels of anthocyanin (8.83±0.03 mg/extract g) were obtained in the Unina cultivar. High ABTS and DPPH radical scavenging activities were determined in the Lover Shy cultivar (428.10±0.29 and 419.59±3.12 mg AAE/extract g, respectively). Taken together, these results indicate that the antioxidant activity and functional components of Lover Shy cultivar are superior, and this extract can potentially be applied as a food and cosmetic ingredient.

비수리(Lespedeza cuneata G. Don) 메탄올 추출물로부터 분획된 용매분획물의 항산화활성과 α-Glucosidase 저해활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

비수리의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성, superoxide dismutase 유사활성, α-glucosidase 저해활성 등에 대해 검토하였다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획 등 순차적 용매분획물의 총 폴리페놀 함량은 각각 12.44, 3.61, 6.39, 27.11, 20.00 및 9.32 ㎎ GAE/g extract residue(ER)로 나타났으며, 총 플라보노이드 함량은 각각 2.94, 9.92, 7.77, 9.27, 5.11 및 2.66 ㎎ CE/g ER, 총 탄닌 함량은 각각 8.75, 10.04, 7.42, 17.32, 11.65 및 7.61 ㎎ TAE/g ER, 총 프로안토시아니딘의 함량은 346.09, 63.50, 103.76, 288.62, 231.99 및 358.48 ㎍ CE/g ER로 나타났다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 DPPH radical 소거활성은 각각 20.62, 5.16, 9.29, 20.80, 20.00 및 20.79 ㎎ TE/g ER, ABTS radical 소거활성은 각각 33.86, 9.24, 17.36, 33.76, 33.49 및 33.86 ㎎ TE/g ER로 나타났다. SOD 유사활성은 각각 4.12, 0.61, 2.01, 9.89, 13.47 및 11.82 unit/mL의 활성을 보여 butanol 분획과 물 분획에서 유의적으로 높은 활성을 나타내었고 α-glucosidase 저해활성은 물 분획 50 및 25 ㎍/mL에서 각각 93.85 및 61.64%로 높은 저해활성을 보이는 것으로 나타났다. 이상의 결과에서 비수리 추출물은 항산화성분 및 항산화활성, SOD 유사활성, α-glucosidase 저해활성을 가지는 물질을 함유한 것으로 보이며, 체내 및 식품에서 활성산소 종 제거에 도움을 줄 수 있을 것으로 보인다. The purpose of this study was to evaluate the antioxidant contents and antioxidant activities of solvent fractions from methanolic extract of sericea lespedeza. To determine the antioxidant compounds in solvent fractions from methanolic extract, total polyphenolic, flavonoid, tannin, and proanthocyanidin contents were measured by spectrophotometric methods. Solvent fractions were evaluated for antioxidative capacity according to DPPH and ABTS radical scavenging activities. Total polyphenolic contents were 12.44, 3.61, 6.39, 27.11, 20.00, and 9.32 ㎎ gallic acid equivalent (GAE)/g extract residue (ER), respectively. Total flavonoid contents were 2.94, 9.92, 7.77, 9.27, 5.11, and 2.66 ㎎ catechin equivalent (CE)/g ER, respectively. Total tannin contents were 8.75, 10.04, 7.42, 17.32, 11.65, and 7.61 ㎎ tannic acid equivalent (TAE)/g ER, respectively. Total proanthocyanidin contents were 346.09, 63.50, 103.76, 288.62, 231.99, and 358.48 ㎍ CE/g ER, respectively. DPPH radical scavenging activities of solvent fractions from methanolic extract of sericea lespedeza were 20.62, 5.16, 9.29, 20.80, 20.00 and 20.79 ㎎ Trolox equivalent (TE)/g ER, and ABTS radical scavenging activities were 33.86, 9.24, 17.36, 33.76, 33.49, and 33.86 ㎎ TE/g ER, respectively. SOD-like activities were 4.12, 0.61, 2.01, 9.89, 13.47, and 11.82 units/mL, and α-glucosidase inhibition activities were 93.85 and 61.64% at concentrations of 50 and 25 ㎍/mL in the water fraction, respectively. The results of this study show that notable antioxidant activities in sericea lespedeza have significant health benefits.

맨드라미(Celosia cristata L.) 꽃 메탄올 추출물로부터 용매분획된 분획물의 항산화활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

맨드라미꽃의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성에 대해 검토하였다. 맨드라미꽃의 항산화성분과 활성은 메탄올 추출물과 순차적 용매분획물에 대해 측정하였으며, 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 추출수율은 각각 23.33, 10.27, 20.00, 13.63, 17.55 및 38.54%였다. 맨드라미 꽃 메탄올 추출물의 총 polyphenol, flavonoid 및 tannin 함량은 각각 6.80 ㎎ GAE/g ME, 2.34 ㎎ CE/g ME 및 6.23 ㎎ TAE/g ME였다. 순차적 용매분획물 중 butanol 분획에서 각각 14.92 ㎎ GAE/g SF, 5.44 ㎎ CE/g SF 및 13.38 ㎎ TAE/g SF로 함량이 높았다. 총 proanthocyanidin 함량은 44.72 ㎍ CE/g ME였고 순차적 용매분획물은 각각 42.47, 44.43, 50.03, 49.12 및 41.80 ㎍ CE/g ER로 용매간 유의적인 차이를 보이지 않았다. 맨드라미꽃의 메탄올 추출물의 DPPH 및 ABTS radical 소거활성은 각각 5.24 및 10.70 ㎎ TE/g ME였고 butanol 분획에서 각각 12.53 및 21.09 ㎎ TE/g SF로 활성이 높았다. 메탄올 추출물의 SOD 유사활성은 7.96 unit/mL로 나타났고 hexane, chloroform, ethyl acetate, butanol 및 물 분획물은 각각 4.56, 6.15, 8.07, 12.36 및 5.21 unit/mL로 butanol 분획의 활성이 높았다. 맨드라미꽃 추출물의 항산화성분과 항산화활성 간에 높은 정의 상관관계를 보였고 맨드라미꽃 추출물이 체내 및 식품에서 유익한 도움을 줄 수 있을 것으로 생각된다. The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of cockscome flowers. The yield of methanolic extracts from cockscome flowers was 23.33%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 10.27, 20.00, 13.63, 17.55, and 38.54%, respectively. Total polyphenolic, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 6.80 ㎎ gallic acid equivalent (GAE)/g ME, 2.34 ㎎ catechin equivalent (CE)/g ME, 6.23 ㎎ tannic acid equivalent (TAE)/g ME, and 44.72 ㎍ CE/g ME, respectively. The highest total polyphenolic, flavonoid, and tannin contents of solvent fractions were 14.92 ㎎ GAE/g solvent fraction (SF), 5.44 ㎎ CE/g SF, and 13.38 ㎎ TAE/g SF in the butanol fraction, respectively. The total proanthocyanidin contents were 42.47, 44.43, 50.03, 49.12, and 41.80 ㎍ CE/g SF, respectively. The DPPH and ABTS radical scavenging activities from cockscome flowers were 5.24 and 10.70 ㎎ Trolox equivalent (TE)/g ME, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 12.53 and 21.09 ㎎ TE/g SF in the butanol fraction, respectively. SOD-like activities of methanolic extracts from cockscome flowers were 7.96 units/mL, whereas those of its solvent fractions were 4.56, 6.15, 8.07, 12.36, and 5.21 units/mL, respectively. The results of this study show that notable antioxidant activities in cockscome flowers have significant health benefits.

캠기반 요골동맥 펄스 시뮬레이터 개발

양태헌 韓國交通大學校 2022 한국교통대학교 논문집 Vol.57 No.-

The goal of this study is to develop a small and economical pulsation simulator that can generate various radial artery pressure waveforms. For this, many pulse waveforms were collected from the subjects using a robotic pulse pressure measurement system, and standard pulse waveforms of the subjects were defined from the data collected using a mathematical model. This standard pulse waveform was used to design and fabricate the cam profile. Using the fabricated cam, a pneumatic pulse waveform generating system based on DC motor and cam-follower mechanism was developed. The developed pulsation simulator was developed to include a mechanism to control the magnitude of the pulse pressure and heart rate, and a real-time pulse display. To verify the performance of the developed pulsation simulator, a series of tests were performed on the waveform generated by the pulsation simulator while varying the pressure level and heart rate using a robotic pulse pressure measurement system. As a result of the radial augmentation index (AI) and phase analysis between the human standard pulse waveform and the waveform generated by the simulator, it was confirmed that the error ratio was small enough that the proposed simulator can generate a human pulse waveform.

요골동맥 혈압파형 재현을 위한 의료로봇 시뮬레이터 개발

양태헌 한국교통대학교 2019 한국교통대학교 논문집 Vol.54 No.-

Radial pulsation waveforms in human is a surrogate maker of estimating the central versus arterial pressure waveforms that are clinically essential for predicting cardiovascular disease. Due to technical limitations, the ability to transfer between central and radial pressures has not been reliably proven. Therefore, to verify the mathematical function between the central pressure and the radial pressure, we developed a prototype radial pulsation simulator that mimics the real human cardiovascular system. The proposed simulator consists of a ventricle, an atrium, arteries and precision sensing equipment. When viscous fluid is injected into the ventricles, the resulting forward wave encounters a unique fluid at the branch of the artery. The reflected wave from the branch propagated backwards, increasing the pressure. The resulting central pressure waveform with extended shoulders was delivered to the radial and ulnar arteries. As a result of the experiments, the developed radial pulsation simulator can now produce radial pressure waveforms like humans. The results suggest that the developed simulator is likely to generate several pressure waveforms representing cardiovascular disease, and that the proposed simulator can be applied to verify the generalized transfer function between the central and radial pressure waveforms.

Electrostatic Resonance 초박형 햅틱 액추에이터 어레이 개발

양태헌 한국교통대학교 2021 한국교통대학교 논문집 Vol.56 No.-

Vibrotactile feedback is a key feature of modern touch displays, greatly improving the user's operability and immersion when interacting with the on-screen interface. In this study, we intend to propose a vibrotactile actuator suitable for mounting on the back of a medium or large display because it can generate strong vibration output by applying an electrostatic force-based mechanism and can be manufactured in a thin flat panel type. The proposed actuator has been developed in a structure that can amplify the vibration force by alternating the electrically grounded mass with electrostatic force by the upper and lower electrodes spaced apart from the radial plate spring. As a result of the performance evaluation, the developed bar-type module with two built-in actuators showed excellent vibration output of up to 14 g at 135 Hz, confirming the possibility of providing haptic feedback in medium and large-sized touch displays.

강낭콩의 식물체 부위와 생육시기에 따른 무기영양성분 함량과 흡수량에 대한 질소의 사용효과

오태근,이경아,송범헌 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.2

This study was conducted to examine the nitrogen effects on uptake and utilization of major mineral nutrients of the kidney bean (Phaseolus vulgaris L.). The N treatments were five levels, 0, 3, 6, 9, 12 N kg/10a. The kidney bean was cultivated on the upland experimental field in the agricultural ???? research & extensions service of Chungbuk province. The contents and uptake amounts of mineral nutrients were investigated throughout the growth stages of the kidney bean. Higher content of total N in the stem was maintained until 8 days before flowering stage and then decreased gradually. Also, total N content of leaves was increased in the vegetative growth stage, and it was decreased rapidly to 8 days after the flowering stage, and then increased gradually to the initial maturity stage. Total N content of roots was tended to decrease until 8 days after the flowering stage, while that of seed was increased from the flowering stage to the maturity stage. The amount of uptake N of the shoot parts was increased until the flowering stage, maintained similarly from the flowering to 14 days after the flowering stage, and then increased rapidly to the maturity stage, while that of root was increased slowly to the flowering stage and then maintained similarly to the maturity.

초음파 가이드를 통한 맥락막상강출혈의 치료

이기헌,유재호,김태원,이상준,김신동 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

Purpose : Intraoperative suprachoroidal hemorrhage is one of the most serious complications. We report a case of suprachoroidal hemorrhage during phacoemulsification surgery, was managed properly. Methods : Suprachoroidal hemorrhage occurred during phacoemulsificaiton in other hospital. The patient was referred to our department immediately after closing the surgical wound. On initial examination, iris and vitreous were prolapsed through surgical wound. Ciliary body was closely place to anterior chamber, and retina was not visible. Results : Prolapased ocular tissue were reduced by emergency operation. We controlled the patient’s intraocular pressure and inflammation. Ultrasonography was repeatedly performed to check the degree of liquefaction of suprachoroidal hemorrhage. After 9 days of suprachoroidal hemorrhage onset, vitrectomy and drainage through sclera was performed. Postoperative state was stable with successful removal of suprachoroidal hemorrhage. After 2 weeks of secondary operation, retinal detachment has occurred on nasal retinal area, so we performed scleral buckling and endolaser photocoagulation. After 6 months, retina was attached well and intraocular pressure was in normal range. Conclusion : In suprachoroidal hemorrhage, drainage of suprachoroidal hemorrhage after liquefaction of thrombi is effective. After successful drainage, postoperative complications may accompany such as retinal detachment or elevation of intraocular pressure. 목적 : 술중 발생하는 맥락막상 출혈은 매우 심각한 합병증이다. 저자는 수정체 유화술 중에 발생한 맥락막상 출혈을 적절히 대처한 증례를 보고하는 바이다. 대상과 방법 : 수정체 유화술 중 발생한 맥락막상강 출혈환자가 수술창을 봉합 후 즉시 본원으로 전원되었다. 초진시 홍채와 유리체가 수술창을 통해 탈출되어 있었으며, 전방을 통해 모양체가 확인되었으나, 망막은 보이지 않았다. 탈출된 안구내 조직을 응급수술을 통해 복원 후 환자의 안압과 염증을 조절하였다. 초음파를 반복적으로 실시하여 맥락막상 출혈의 액화 정도를 확인하였으며, 출혈 9일 후 유리체 절제술과 공막을 통한 배액술을 시행하였다. 결과 : 맥락막상 출혈이 성공적으로 제거되었으며 술 후 상태는 안정적이었다. 수술 2주 후 비측 망막 박리가 발생하여 공막돌륭술과 안구내 삽관 레이저술을 시행하였다. 6개월 후 망막은 잘 붙어있었으며, 안압은 정상 범위를 유지하였다. 결론 : 맥락막상 출혈의 경우 출혈의 배액 시기를 결정하는 것이 어렵지만, 규칙적인 초음파를 통해 배액술의 시기를 결정할 수 있다. 또한 맥락막상강 출혈의 성공적인 배액 이후에도 망막박리 및 안압상승과 같은 합병증의 동반에 대해 유념해야한다.

그래핀을 이용한 Fe 처리에 관한 연구

함헌,이영진,전태성 한국교통대학교 2014 한국교통대학교 논문집 Vol.49 No.-

Graphene has not only outstanding electrical characteristics which has structural and chemical safty, but has many phisical, chemical and mechanical merits unaffected by surrounding environment. With remarkable physical, chemical and electrical properties of graphene, graphaene can possible to use in wide range of industrial applications, including energy storage, gas detection conducting electrodes, heavy metal removals and etc.. In this paper, Graphene has been chemical surface processed to absorb dissolved iron in the wastewater and Fe removal efficiency showed 90.4% in 100mg/L Fe concentration