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사람 대장암 세포주에서 Diallyl Disulfide의 세포증식억제 및 Apoptosis 유도 효과
김태명(Tae Myoung Kim),류재면(Jae Myun Ryu),권현정(Hyun Jung Kwon),우관식(Koan Sik Woo),정헌상(Heon Sang Jeong),홍진태(Jin Tae Hong),김대중(Dae Joong Kim) 한국독성학회 2005 Toxicological Research Vol.21 No.4
Epidemiological and laboratory studies provide insight into the anti-carcinogenic potential of garlic and its constituent compounds. Garlic is appealing as an anti-carcinogenic agent due to its ability to induce apoptosis in vitro. Diallyl disulfide (DADS) is one of the major components of garlic that used to determine inhibition of cell proliferation and induced apoptosis in human colon cell lines. In this study, human colorectal cancer cell lines (LOVO, HCT-116, SW-480) were exposed to DADS. The inhibitory effects of DADS dose level more than 50 μM in the cell viability of all cell lines. Cell growth activity inhibits of human colon cancer cell lines. The inhibitory effects of DADS dose level more than AFG₁5~50 μM in the cell growth using MTT assay. We found that DADS may have the apoptosis action (chromatin condensation, DNA fragmentation) using DAPI staining and increased the expression of caspase-3 at the dose level more than 100 μM, decreased the expression level of β- catenin at dose dependent in the western blotting. We suggest that DADS may have a potential candidate as cancer chemopreventive agents.
1,2-Dimethylhydrazine에 의해 유발된 Colonic Aberrant Crypt Foci에 대한 마늘추출물의 암예방효과
김태명(Tae Myoung Kim),류재면(Jae Myun Ryu),권현정(Hyun Jung Kwon),황인국(In Guk Hwang),반정옥(Jung Ok Ban),정헌상(Heon Sang Jeong),홍진태(Jin Tae Hong),김대중(Dae Joong Kim) 한국독성학회 2007 Toxicological Research Vol.23 No.2
Garlic (Allium sativum L.) with the food supplement material and medicine was used traditionally in Asia and Europe. Epidemiological studies revealed that the intake of garlic reduced incidences of various cancer including digestive system. The present study was designed to investigate the effect of garlic ethanol extract on the development of colonic aberrant crypt foci (ACF) induced by 1,2-dimethylhydrazine (DMH) in male F344 rats. Five-week-old rats were given four times for two weeks to subcutaneous injections by DMH (30 ㎎/㎏ body weight) to induce ACF. The animals were divided into groups that fed diet containing garlic ethanol extract at five different doses (0.1, 0.2, 0.5, 2, 5%), respectively, animals were evaluated for the total number of ACF and total aberrant crypts (AC) per colon detected from methylene blue-stained rat colon. ACF were formed in animals in DMH-treated group. The feeding suppressed potently the appearance ACF in the colon of rats. Especially, fed diet containing garlic ethanol extract at intermediate dose (0.5%) significantly reduced the number of ACF and AC per colon (p < 0.05). Garlic ethanol extract inhibited DMH-induced overexpression of Activator Protein-1 (AP-1) and β-catenin genes related to cell proliferation that also upregulated the expression of p21Waf1/Cip1 mRNA, a cell cycle-regulating gene. These results suggested that garlic ethanol extract may inhibit ACF formation, β-catenin gene as the early preneoplastic marker of malignant potential in the process of colon carcinogenesis.
랫드의 간에서 다양한 농도의 아플라톡신 투여에 의한 DNA Adduct의 형성과 Ras의 발현양상
김태명(Tae Myoung Kim),허진주(Jin Joo Hue),리란(Lan Li),김대중(Dae Joong Kim),남상윤(Sang Yoon Nam),윤영원(Young Won Yun),이범준(Beom Jun Lee) 한국독성학회 2005 Toxicological Research Vol.21 No.4
Aflatoxins are produced by Aspergillus flavus, parasiticus that grows in improperly stored cereals. Aflatoxin B₁ (AFB₁) is a potent hepatocarcinogen in a variety of experimental animals including human beings. In spite of a high attention to the hepatocarcinogenecity of aflatoxins, the relative toxicity of other types (AFB₂, AFG₁ and AFG₂) of the toxins is not fully clarified. Sprague-Dawley male rats were orally administered with AFB₁, AFB₂, AFG₁ and AFG₂ at the dose of 250, 1250, and 2500 μg/kg body weight. Animals were then killed at 12, 24 or 48 hrs following aflatoxin adminstration. Subsequently the relative weight of liver was measured and histopathological examination on the liver was performed. Level of 8-OxodG and expression of ras gene in the liver was determined. The relative liver weights at high doses of AFB₁ and AFG₁ was significantly low. The treatment of AFB₁ at the high dose of 2500 ㎍/㎏ showed vacuolar degeneration and centrilobular hepatic necrosis with inflammatory cells. The pathological changes by AFB₂, AFG₁ and AFG₂ were not clearly found. The formation of 8-OxodG by AFB₁ increased in a dose-dependent manner up to 24 hrs after a single treatment of AFB₁ thereafter decreased to the level of the control. The treatments of AFB₂, AFG₁ and AFG₂ showed an inconsistent pattern in the formation of 8-OxodG in the liver of rats with increasing time. The expression of ras oncogene in the liver by AFB₁ at the dose of 1250 ㎍/㎏ was increased twice compared to the control. The treatments of AFB₂, AFG₁ and AFG₂ at all doses decreased the expression of ras in the liver. These results in the present study indicate that AFB₁ among aflatoxins with low comparable levels is the most toxic as determined by early biomarkers such as 8-OxodG formation and ras expression. However, the levels of 8-OxodG and ras as biomarkers were not useful to predict the relative hepatocarcinogenicity of aflatoxins to AFB₁ in the present model. Further studies are required to look for other biomarkers to predict carcinogenic potency of aflatoxins.
김태명,지석근,김영완,Kim, Tae-Myoung,Jee, Suk-Kun,Kim, Young-Wan 한국전기전자학회 2018 전기전자학회논문지 Vol.22 No.3
본 논문에서는 옥내 외에 설치 운영되는 수배전반의 결로 현상을 방지하는 IT 기반 자동 온습도 제어기를 설계하고 제작하였다. 이슬 결로점 온도가 실내공간의 습도와 온도로부터 산출되고, 이슬 결로점 온도와 결로 부위의 온도를 비교하여 수배전반의 결로 현상을 방지할 수 있는 시설이 순차적으로 제어 동작되도록 구현하였다. 아울러 원격지의 수배전반의 결로 기반 제어기 동작 상태를 모니터링 할 수 있는 원격 모니터링 장치를 LoRa 무선통신 방식을 사용하여 구현하였다. 제어기와 원격 모니터링 장치를 설치 운영하여 전송되는 데이터의 정확성과 결로 기반 제어기 동작상태의 안정성을 확인하였다. 본 제어 장치는 수배전반 이외에 결로가 발생되는 장비에 설치되어 결로를 방지할 수 있는 장치로 용이하게 활용될 수 있다. IT-based automatic controller that control the temperature and humidity to prevent dew condensation of distributing board was designed and implemented in this paper. The dew condensation temperature was deduced from room temperature and humidity of distributing board. Based on the comparisons between the deduced dew condensation temperature and the temperature of surface condensation, the facilities that can prevent the condensation was implemented to be operated in due order. Also, the remote monitoring module to monitor operation status of controller was implemented using LoRa technique. The performances for controller operation and data transmission were validated from the transmission and operation test for dew condensation prevention. The controller can be put to good use at the facilities that requires the condensation prevention.
아플라톡신을 간회 투여한 랫드의 간에서 CYP450 1A1, p53의 발현과 DNA adduct의 형성
이범준,이숙진,김태명,김대중,남상윤,현상환,강종구,홍진태,김철규,윤영원,Lee, Beom Jun,Lee, Sook Jin,Kim, Tae Myoung,Kim, Dae Joong,Nam, Sang Yoon,Hyun, Sang Hwan,Kang, Jong Koo,Hong, Jin Tae,Kim, Cheul Kyu,Yun, Young Won 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4
Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus that grow in improperly stored cereals. Aflatoxin B1 ($AFB_1$) is a potent hepatocarcinogen in a variety of experimental animals including human beings. In spite of a high attention to the hepatocarcinogenecity of $AFB_1$, the relative toxicity of aflatoxins ($AFB_2$ and $AFG_1$) is not fully clarified. Sprague-Dawley male rats were orally administered with $AFB_1$, $AFB_2$, and $AFG_1$ at the dose of 250 ${\mu}g/kg$ (additionally including a dose of $1250{\mu}g/kg $ for $AFB_1$) body weight. Animals were then killed at 12, 24 or 48 hrs following aflatoxin exposure. Subsequently the immunohistochemical examination of p53, cytochrome p450 1A1 (CYP450 1A1), and glutathione-S-transferase placental form (GST-P) were performed. The level of the 8-OxodG in the liver was determined. Expressions of CYP450 1A1 and p53 were high in the liver of rats through 48 hrs after treatment of $AFB_1$ at the single dose of $250{\mu}g/kg $. This pattern was more clear as increasing doses. The treatment of $AFB_2$ and $AFG_1$ did not affect the expression of CYP450 1A1 but it caused weak expression of p53. The activity of GST were not found in the liver of rats treated with aflatoxins. The formation of 8-OxodG by $AFB_1$ increased in a dose-dependent manner up to 24 hrs after a single treatment of $AFB_1$ thereafter decreased to the level of control. The treatment of $AFB_2$ and $AFG_1$ did not affect the levels of 8-OxodG in the liver of rats with increasing time. These results in the present study indicate that $AFB_1$ among aflatoxins with low comparable levels is the most toxic as determined by early biomarkers such as CYP450 1A1, p53, GST-P, and 8-OxodG.
발아 벼 부위별 추출물의 항산화활성과 Angiotensin Converting Enzyme I 저해활성
김현영,황인국,김태명,박동식,김재현,김대중,이준수,정헌상,Kim, Hyun-Young,Hwang, In-Guk,Kim, Tae-Myoung,Park, Dong-Sik,Kim, Jae-Hyun,Kim, Dae-Joong,Lee, Jun-Soo,Jeong, Heon-Sang 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.6
한국산 벼의 발아 전과 후의 부위별 및 추출용매별 항산화성분 및 활성변화를 살펴보았다. 발아 전과 후의 벼를 왕겨, 현미 및 싹으로 분리하고 각각 열수 및 70% 에탄올을 이용하여 추출하였다. 총 폴리페놀 함량은 현미 물추출물에서 발아 전 1.67에서 발아 후 5.84 mg/g으로 증가하였으며, DPPH 라디칼소거능은 벼 에탄올추출물에서 발아 전 22.95 %에서 발아 후 31.32%로 증가하였지만 왕겨와 현미 부분에서는 발아 후 감소하였다. 총 항산화력은 에탄올추출물에서 발아 전과 후에 큰 차이를 보이지 않았으나 싹 부분에서는 4.41 mg AA eq/g으로 높게 나타났다. 환원력은 에탄올 추출물 현미에서 발아 전 0.32에서 발아 후 0.45로 증가하였다. ACE 저해활성은 왕겨에서 가장 높았으며 발아 후 증가하였다. 본 연구결과 발아 시 항산화활성이 증가하는 원인에 대한 추후 연구가 필요하다 판단된다. We investigated the changes in antioxidant and angiotensin converting enzyme I (ACE) inhibitory activity in different parts of 'Ilpum' (Oryza sativa L.) rough rice before and after germination. Rough rice, either before or after germination, were separated into hull, brown rice, or sprout, and then extracted with distilled water and 70% ethanol. After germination, the total polyphenol contents of the distilled water extract of the brown rice was higher than before germination (5.84 and 1.67 mg/g, respectively). The DPPH radical scavenging activity on the unseparated rough rice ethanol extract increased from 22.95% before germination to 31.32% after germination, but it decreased in both the hull and brown rice extracts. The ABTS radical scavenging activity was highest in the sprout extract with a value of 4.41 mg AA eq/g. The reducing power of the brown rice ethanol extract increased from 0.32 before germination to 0.45 after germination. The ACE inhibitory activity of the in hull ethanol extract increased after germination. These results indicate that antioxidant capacity increases in the hull and sprout after germination could be considered having significant health benefits.
발아 벼 열수 및 에탄올 추출물의 암세포주 증식억제 효과
김현영(Hyun Young Kim),황인국(In Guk Hwang),김태명(Tae Myoung Kim),김대중(Dae Joong Kim),박동식(Dong Sik Park),김재현(Jae Hyun Kim),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.8
발아 전후 6종의 벼(일품벼, 백진주벼, 설갱벼, 고아미2, 거대배아벼 및 흑광벼)의 물 및 에탄올 추출물에 대한 암세포주 성장억제효과를 간암주(HepG2), 전립선암세포주(PC-3) 및 유방암세포주(MCF-7)에 대하여 살펴보았다. 물추출물은 1 mg/mL 농도에서 3종의 암세포주에 대하여 118.97~44.85% 범위의 높은 생존율을 보여 항암효과가 낮 았지만 에탄올 추출물은 90.82~5.04% 범위의 생존율을 보여 물 추출물보다 높은 암세포 성장억제 효과를 나타내었다. 일품벼 발아 전후 에탄올 추출물은 간암세포주에 대하여 1mg/mL 농도에서 각각 27.23 및 42.36%의 생존율을 보여 발아 시 항암효과가 증가하였으며, 특히 유방암세포주에 대해서는 0.25 mg/mL 농도에서 각각 48.20 및 56.82%의 생존율을 보여 다른 품종보다 성장억제율이 높았다. 또한 설갱벼 발아 전후 에탄올 추출물은 전립선암세포에 대하여 1 mg/mL 농도에서 각각 44.52 및 64.51%의 생존율을 보여 발아 항암효과가 증가하였다. 추후 항암활성 물질의 분리 동정과 메커니즘 규명에 대한 연구가 수행되어야 할 것으로 판단된다. This study was conducted to investigate the effect of 70% ethanol and water extract of Korean rough rice (‘Ilpum’, ‘Goami2’, ‘Keunnun’, ‘Sulgaeng’, ‘Baegjinju’, and ‘Heugkwang’) before and after germination on proliferation of human cancer cell lines (HepG2, PC-3, and MCF-7). Antiproliferation effect was higher in ethanol extract than water extract, and was higher in after germination. ‘Ilpum’ ethanol extract after germination showed higher anti-proliferation effect on HepG2 and PC-3 cell lines than before germination. The cell viability on HepG2 and PC-3 cell lines of ‘Ilpum’ ethanol extract after germination was 27.23% and 5.05% at 1.0 ㎎/mL, respectively. The anti-proliferation effect on MCF-7 was the highest in ‘Ilpum’ and ‘Heugkwang’ 70% ethanol extract after germination and those cell viabilities were 7.27% and 17.00% at 0.5 ㎎/mL, respectively. These results suggest that germinated rough rice might have a potentially preventive effect on human cancer cells.