<i>Rhodanobacter koreensis</i> sp. nov., a bacterium isolated from tomato rhizosphere

Won, KyungHwa,Singh, Hina,Ngo, Hien T. T.,Son, HeungMin,Kook, MooChang,Kim, Ki&#x2013,Young,Yi, Tae&#x2013,Hoo International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.4

<P>A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7<SUP>T</SUP>, was isolated from tomato plant rhizosphere soil. Strain THG-DD7<SUP>T</SUP> grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 0.5 % (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7<SUP>T</SUP> was most closely related to <I>Rhodanobacter umsongensis</I> GR24-2<SUP>T</SUP> (98.2 %), <I>Rhodanobacter panaciterrae</I> LnR5-47<SUP>T</SUP> (98.0 %), <I>Rhodanobacter soli</I> DCY45<SUP>T</SUP> (97.9 %), <I>Rhodanobacter terrae</I> GP18-1<SUP>T</SUP> (97.9 %) and <I>Dyella ginsengisoli</I> Gsoil 3046<SUP>T</SUP> (97.7 %). The DNA G+C content was 65.2 mol%. In DNA–DNA hybridization, the DNA relatedness levels between strain THG-DD7<SUP>T</SUP> and its closest phylogenetically neighbours were below 40.0 %. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-<I>N</I>-methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, anteiso-C<SUB>15 : 0</SUB> and iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and/or C<SUB>16 : 0</SUB> 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7<SUP>T</SUP> to the genus <I>Rhodanobacter</I> . The results of physiological and biochemical tests enabled strain THG-DD7<SUP>T</SUP> to be differentiated genotypically and phenotypically from the species of the genus <I>Rhodanobacter</I> with validly published names. Therefore, the novel isolate represents a novel species, for which the name <I>Rhodanobacter koreensis</I> sp. nov. is proposed. The type strain is THG-DD7<SUP>T</SUP> ( = KACC 17650<SUP>T</SUP> = JCM 19614<SUP>T</SUP>).</P>

Influence of Cr Doping on the Critical Behavior of Amorphous Alloy Ribbons Fe_{78–<i>x</i>}Cr_<i>x</i>Si₄Nb₅B₁₂Cu₁

Phan, T. L.,Thanh, P. Q.,Chau, N.,Huu, C. X.,Ngo, D.-T,Ho, T. A.,Thanh, T. D.,Yu, S. C. IEEE 2014 IEEE transactions on magnetics Vol.50 No.11

<P>Though many previous works focused on studying Cr-doped Fe-Si-Nb-B-Cu amorphous alloys, magnetic-interaction mechanisms in these materials have not been carefully investigated yet. Dealing with these issues, we have prepared the amorphous alloy ribbons Fe<SUB>78-x</SUB>Cr<SUB>x</SUB>Si<SUB>4</SUB>Nb<SUB>5</SUB>B<SUB>12</SUB>Cu<SUB>1</SUB> with x= 1, 3, and 6, and then studied their magnetic and critical properties. Magnetization versus temperature and magnetic-field measurements, MHT, performed on a vibrating sample magnetometer reveal that the Cr-content increase in Fe<SUB>78-x</SUB>Cr<SUB>x</SUB>Si<SUB>4</SUB>Nb<SUB>5</SUB>B<SUB>12</SUB>Cu<SUB>1</SUB> reduces the T<SUB>C</SUB> from 430 K for x= 1 to about 322 K (for x= 6). This indicates the decline of ferromagnetic (FM) exchange interactions between Fe atoms when there is the presence of Cr atoms. We have also analyzed the M(H) data at the temperatures in the vicinity of the T<SUB>C</SUB> using the modified Arrott plot method and the scaling hypothesis, and determined the values of the critical exponents β = 0.367-0.376 and γ = 1.315-1.338. These values are close to those expected for the 3-D Heisenberg model with β = 0.365 and γ = 1.336, proving the existence of short-range FM order in the amorphous alloy ribbons.</P>

Inhomogeneous Ferromagnetism and Spin-Glass-Like Behavior in <inline-formula> <tex-math notation='TeX'> <tex> $({\rm Nd}_{1-x}{\rm Y}_{x})_{0.7}{\rm Sr}_{0.3}{\rm MnO}_{3}$ </tex> </tex-math></inline-formula> With <inline-formula> <tex-math notation='TeX

Phan, T. L.,Nguyen, V. D.,Ho, T. A.,Khiem, N. V.,Thanh, T. D.,Phuc, N. X.,Thang, P. D.,Yu, S. C. IEEE 2014 IEEE transactions on magnetics Vol.50 No.6

<P>The magnetic properties of polycrystalline ceramic samples (Nd1-xYx)(0.7)Sr0.3MnO3 with x = 0.21 - 0.35 were studied by means of dc magnetization and ac susceptibility measurements. Experimental results reveal a strong decrease of the ferromagnetic (FM)-paramagnetic phase-transition temperature (T-C) from 97 to 65 K as increasing x from 0.21 to 0.35, respectively. There is magnetic inhomogeneity associated with short-range FM order. Particularly, the samples undergo a spin-glass (SG) phase transition at the so-called blocking temperature (T-B) below T-C, which shifts toward lower temperatures with increasing the applied field, Hex; T-B -> T-g (the SG phase-transition temperature) as H-ex -> 0. The existence of the SG behavior in these samples was also confirmed by frequency (f) dependences of the ac susceptibility. For the in-phase/real component, chi' (T), it shows a frequency-dependent peak at the SG freezing temperature (T-f); T-f -> T-g as f -> 0. Dynamics of this process were analyzed by means of the slowing down scaling law, tau/tau(0) proportional to (T-f/T-g - 1)(-zv), where tau(0) and zv are the characteristic time and critical exponent, respectively. Fitting the experimental T-f(f) data to the scaling law gave the results of zv = 10.1-12.3 and tau(0) = 10(-21)-10(-15)s. These values are different from those expected for canonical SG systems with zv = 10 and tau(0) = 10(-13)s, revealing the cluster-SG behavior of (Nd1-xYx)(0.7)Sr0.3MnO3 samples. Notably, the increase in Y content leads to the shift of tau(0) and zv values toward those of canonical SG systems, which is ascribed to an expansion of SG clusters.</P>

NEDD 4‐induced degradative ubiquitination of phosphatidylinositol 4‐phosphate 5‐kinase α and its implication in breast cancer cell proliferation

Tran, Mai Hoang,Seo, Eunjeong,Min, Soohong,Nguyen, Quynh&#x2010,Anh T.,Choi, Juyong,Lee, Uk&#x2010,Jin,Hong, Soon&#x2010,Sun,Kang, Hyuk,Mansukhani, Alka,Jou, Ilo,Lee, Sang Yoon John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.9

<P><B>Abstract</B></P><P>Phosphatidylinositol 4‐phosphate 5‐kinase (PIP5K) family members generate phosphatidylinositol 4,5‐bisphosphate (PIP2), a critical lipid regulator of diverse physiological processes. The PIP5K‐dependent PIP2 generation can also act upstream of the oncogenic phosphatidylinositol 3‐kinase (PI3K)/Akt pathway. Many studies have demonstrated various mechanisms of spatiotemporal regulation of PIP5K catalytic activity. However, there are few studies on regulation of PIP5K protein stability. Here, we examined potential regulation of PIP5Kα, a PIP5K isoform, via ubiquitin‐proteasome system, and its implication for breast cancer. Our results showed that the ubiquitin ligase NEDD4 (neural precursor cell expressed, developmentally down‐regulated gene 4) mediated ubiquitination and proteasomal degradation of PIP5Kα, consequently reducing plasma membrane PIP2 level. NEDD4 interacted with the C‐terminal region and ubiquitinated the N‐terminal lysine 88 in PIP5Kα. In addition, PIP5Kα gene disruption inhibited epidermal growth factor (EGF)‐induced Akt activation and caused significant proliferation defect in breast cancer cells. Notably, PIP5Kα K88R mutant that was resistant to NEDD4‐mediated ubiquitination and degradation showed more potentiating effects on Akt activation by EGF and cell proliferation than wild‐type PIP5Kα. Collectively, these results suggest that PIP5Kα is a novel degradative substrate of NEDD4 and that the PIP5Kα‐dependent PIP2 pool contributing to breast cancer cell proliferation through PI3K/Akt activation is negatively controlled by NEDD4.</P>

Effect of Dietary Copper Sources (Cupric Sulfate and Cupric Methionate) and Concentrations on Performance and Fecal Characteristics in Growing Pigs

Huang, Y.,Zhou, T.X.,Lee, J.H.,Jang, H.D.,Park, J.C.,Kim, I.H. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.6

This study was conducted to assess the effects of organic and inorganic copper on performance in growing pigs. A total of 100 pigs, average age 63 d and initial body weight 21.46${\pm}$1.13 kg, were assigned to five treatment groups. Dietary treatments included i) CON (basal diet, 0 ppm Cu), ii) T1 (basal diet with 67 ppm Cu as cupric sulfate, $CuSO_4$), iii) T2 (basal diet with 134 ppm Cu as $CuSO_4$), iv) T3 (basal diet with 67 ppm Cu as cupric methionate, CuMet) and v) T4 (basal diet with 134 ppm Cu as CuMet). Throughout the entire experimental period, ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain: feed) ratios showed no significant differences. The dry matter digestibility was improved in the T1, T2, T3, and T4 treatments (p<0.05), as compared with CON. Nitrogen digestibility was improved in the T3 treatment group as compared with CON (p<0.05). As compared with the T1 treatment group, fecal pH values were improved in the CON, T3, and T4 treatment groups (p<0.05). Fecal Cu concentrations were significantly lower in the CON, T3, and T4 treatment groups than in T1 and T2 (p<0.05). The incidence of diarrhea was reduced when the pigs were fed on the T2, T3, and T4 diets as compared with CON. In conclusion, diets supplemented with 67 or 134 ppm Cu as CuMet may prove effective in improving nutrient digestibility and fecal pH value in growing pigs, and fecal Cu concentrations may be reduced by CuMet supplementation.

OGLE‐2009‐BLG‐023/MOA‐2009‐BLG‐028: characterization of a binary microlensing event based on survey data

Hwang, K.&#x2010,H.,Han, C.,Udalski, A.,Sumi, T.,Gould, A.,Jaroszy&#x144,ski, M.,Kubiak, M.,Szyma&#x144,ski, M. K.,Pietrzy&#x144,ski, G.,Soszy&#x144,ski, I.,Szewczyk, O.,Ulaczyk, K.,Wyrzykowski, &#x13 Blackwell Publishing Ltd 2011 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.413 No.2

<P><B>ABSTRACT</B></P><P>We report the result of the analysis of the light curve of a caustic‐crossing binary‐lens microlensing event OGLE‐2009‐BLG‐023/MOA‐2009‐BLG‐028. Even though the event was observed solely by survey experiments, we could uniquely determine the mass of the lens and distance to it by simultaneously measuring the Einstein radius and lens parallax. From this, we find that the lens system is composed of M‐type dwarfs with masses (0.50 ± 0.07) and (0.15 ± 0.02) M<SUB>⊙</SUB> located in the Galactic disc with a distance of ∼1.8 kpc toward the Galactic bulge direction. The event demonstrates that physical lens parameters of binary‐lens events can be routinely determined from future high‐cadence lensing surveys and thus microlensing can provide a new way to study Galactic binaries.</P>

Sonochemical synthesis of Fe–TiO2â€â€&Arin

Kim, T. H.,GÃ,&#x192,Æ’Ã,&#x201a,Â,³mez-SolÃ,&#x192,Æ’Ã,&#x201a,Â,­s, C.,Moctezuma, E.,Lee, S. W. VSP 2014 Research on chemical intermediates Vol.40 No.4

Asymptotics for self-normalized random products of sums of i.i.d. random variables

Pang, T.X.,Lin, Z.Y.,Hwang, K.S. Academic Press 2007 Journal of mathematical analysis and applications Vol.334 No.2

Let {X,X<SUB>i</SUB>;i>=1} be a sequence of independent and identically distributed positive random variables, which is in the domain of attraction of the normal law, and t<SUB>n</SUB> be a positive, integer random variable. Denote S<SUB>n</SUB>=@?<SUB>i=1</SUB><SUP>n</SUP>X<SUB>i</SUB>, V<SUB>n</SUB><SUP>2</SUP>=@?<SUB>i=1</SUB><SUP>n</SUP>(X<SUB>i</SUB>-X@?)<SUP>2</SUP>, where X@? denotes the sample mean. Then we show that the self-normalized random product of the partial sums, (@?<SUB>k=1</SUB><SUP>t<SUB>n</SUB></SUP>S<SUB>k</SUB>kμ)<SUP>&</SUP>mu;<SUP>V''<SUB>t'n</SUB></SUP>, is still asymptotically lognormal under a suitable condition about t<SUB>n</SUB>.

Using Ultrafast X-ray Spectroscopy To Address Questions in Ligand-Field Theory: The Excited State Spin and Structure of [Fe(dcpp)₂]²⁺

Britz, Alexander,Gawelda, Wojciech,Assefa, Tadesse A.,Jamula, Lindsey L.,Yarranton, Jonathan T.,Galler, Andreas,Khakhulin, Dmitry,Diez, Michael,Harder, Manuel,Doumy, Gilles,March, Anne Marie,Bajno&#x3 American Chemical Society 2019 Inorganic chemistry Vol.58 No.14

<P>We have employed a range of ultrafast X-ray spectroscopies in an effort to characterize the lowest energy excited state of [Fe(dcpp)<SUB>2</SUB>]<SUP>2+</SUP> (where dcpp is 2,6-(dicarboxypyridyl)pyridine). This compound exhibits an unusually short excited-state lifetime for a low-spin Fe(II) polypyridyl complex of 270 ps in a room-temperature fluid solution, raising questions as to whether the ligand-field strength of dcpp had pushed this system beyond the <SUP>5</SUP>T<SUB>2</SUB>/<SUP>3</SUP>T<SUB>1</SUB> crossing point and stabilizing the latter as the lowest energy excited state. Kα and Kβ X-ray emission spectroscopies have been used to unambiguously determine the quintet spin multiplicity of the long-lived excited state, thereby establishing the <SUP>5</SUP>T<SUB>2</SUB> state as the lowest energy excited state of this compound. Geometric changes associated with the photoinduced ligand-field state conversion have also been monitored with extended X-ray absorption fine structure. The data show the typical average Fe-ligand bond length elongation of ∼0.18 Å for a <SUP>5</SUP>T<SUB>2</SUB> state and suggest a high anisotropy of the primary coordination sphere around the metal center in the excited <SUP>5</SUP>T<SUB>2</SUB> state, in stark contrast to the nearly perfect octahedral symmetry that characterizes the low-spin <SUP>1</SUP>A<SUB>1</SUB> ground state structure. This study illustrates how the application of time-resolved X-ray techniques can provide insights into the electronic structures of molecules-in particular, transition metal complexes-that are difficult if not impossible to obtain by other means.</P><P>Time-resolved X-ray emission and absorption spectroscopies have been used to probe the excited-state electronic and geometric structure of an Fe(II) polypyridyl complex. Analysis of the data revealed that the lowest energy excited state is high-spin (<I>S</I> = 2) in character. This determination had not been possible using other experimental techniques (e.g., time-resolved optical spectroscopy), demonstrating the potential for ultrafast X-ray methods to address scientific questions that are difficult to resolve by other means.</P> [FIG OMISSION]</BR>

Serine palmitoyltransferase inhibitor myriocin induces growth inhibition of B16F10 melanoma cells through G₂/M phase arrest

Lee, Y.&#x2010,S.,Choi, K.&#x2010,M.,Choi, M.&#x2010,H.,Ji, S.&#x2010,Y.,Lee, S.,Sin, D.&#x2010,M.,Oh, K.&#x2010,W.,Lee, Y.&#x2010,M.,Hong, J.&#x2010,T.,Yun, Y.&#x2010,P.,Yoo, H.&#x2010,S. Blackwell Publishing Ltd 2011 Cell proliferation Vol.44 No.4

<P><B>Abstract</B></P><P><B>Objectives: </B> Melanoma is the most aggressive form of skin cancer, and it resists chemotherapy. Candidate drugs for effective anti‐cancer treatment have been sought from natural resources. Here, we have investigated anti‐proliferative activity of myriocin, serine palmitoyltransferase inhibitor, in the <I>de novo</I> sphingolipid pathway, and its mechanism in B16F10 melanoma cells.</P><P><B>Material and methods: </B> We assessed cell population growth by measuring cell numbers, DNA synthesis, cell cycle progression, and expression of cell cycle regulatory proteins. Ceramide, sphingomyelin, sphingosine and sphingosine‐1‐phosphate levels were analysed by HPLC.</P><P><B>Results: </B> Myriocin inhibited proliferation of melanoma cells and induced cell cycle arrest in the G<SUB>2</SUB>/M phase. Expressions of cdc25C, cyclin B1 and cdc2 were decreased in the cells after exposure to myriocin, while expression of p53 and p21<SUP>waf1/cip1</SUP> was increased. Levels of ceramide, sphingomyelin, sphingosine and sphingosine‐1‐phosphate in myriocin‐treated cells after 24 h were reduced by approximately 86%, 57%, 75% and 38%, respectively, compared to levels in control cells.</P><P><B>Conclusions: </B> Our results suggest that inhibition of sphingolipid synthesis by myriocin in melanoma cells may inhibit expression of cdc25C or activate expression of p53 and p21<SUP>waf1/cip1</SUP>, followed by inhibition of cyclin B1 and cdc2, resulting in G<SUB>2</SUB>/M arrest of the cell cycle and cell population growth inhibition. Thus, modulation of sphingolipid metabolism by myriocin may be a potential target of mechanism‐based therapy for this type of skin cancer.</P>