Bacteroides fragilis의 독소 유전자 분석 및 발병기전에 관한 연구

오희복,이근영,정경태,성원근 국립보건연구원 2000 국립보건원보 Vol.37 No.-

TRIIODTHYRONINE (T₃) ENHANCES THE STIMULATORY EFFECT OF 1, 25-DIHYDROXYVITAMIN D₃ ON CALBINDIN-D_28k mRNA EXPRESSION IN THE KIDNEY AND INTESTINE BUT NOT IN CEREBELLUM OF THE CHICK

Sechman, A.,Shimada, K.,Saito, N.,Ieda, T.,Ono, T. Asian Australasian Association of Animal Productio 1996 Animal Bioscience Vol.9 No.1

The present study was conducted to investigate the role of thyroid hormones in the regulation of gene expression of calbindin-$D_{28k}$ (CaBP-D28K) in the chicken. By employing slot blot and RIA analyses, levels of CABP-D28K mRNA and CaBP-D28K protein in the intestine, kidney, cerebellum and liver were measured 6 and 12 h after i.m. injection of 1, 25-dihydroxyvitamin $D_3$ [1, 25 $(OH)_2D_3$; 250 ng/chick] and 3, 5, 3'-triiodothyronine ($T_3$; 500 ng/chick) in one-day-old chicks. The abundant messages of CaBP-D28K mRNA were detected in the intestine, kidney and cerebellum while there was little message in the liver. After 1, 25 $(OH)_2D_3$ treatment (6 + 12 hours), levels of CaBP-D28K mRNA increased in the intestine, but there was no change in the mRNA levels in the kidney and cerebellum. Although $T_3$ alone had no effect on CaBP-D28K mRNA levels, simultaneous administration of $T_3$ enhanced the 1, 25 $(OH)_2D_3$ effect of levels of CaBP-D28K mRNA in the intestine both 6 and 12 h post-treatment, and in the kidney 12 h post-treatment. At a protein level, co-treatment with 1, 25 $(OH)_2D_3$ and $T_3$ elicited a significant increase in CaBP-D28K expression in the intestine 12 h post-treatment, as compared to treatment with only 1, 25 $(OH)_2D_3$, whereas no differences were observed in the CaBP-D28K protein levels in the kidney and cerebellum. These results suggest that thyroid hormones may play a synergistic role with 1, 25 $(OH)_2D_3$ for CaBP-D28K gene expression in the intestine and kidney in chicks.

Magnetic and Transport Properties of a New Caged Compound PrOs2Zn20

K. Wakiya,N. Nagasawa,K. T. Matsumoto,T. Onimaru,K. Umeo,T. Takabatake 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.12

The magnetic and transport properties of a Pr-encaged compound PrOs2Zn20 have been studiedby the electrical resistivity (T), magnetic susceptibility ρ(T), and specific heat C(T) measurementsdown to 2 K. The (T) and the C(T) show distinct anomalies at Ts = 87 K, which resemble theanomalies in the isostructural and isoelectronic compound PrRu2Zn20 with the structural phasetransition at Ts = 138 K. Above 50 K, the ρ(T) follows the Curie-Weiss law with the effectivemagnetic moment of 3.64 µB/f.u., which indicates the trivalent state of the Pr ion. The saturatedbehavior of ρ(T) below 5 K indicates the Van-Vleck paramagnetic state. Hence, the Pr3+ ion fallsin a nonmagnetic ground state under the crystalline electric field.

Two-stage partial melting and contrasting cooling history within the Higher Himalayan Crystalline Sequence in the far-eastern Nepal Himalaya

Imayama, T.,Takeshita, T.,Yi, K.,Cho, D.L.,Kitajima, K.,Tsutsumi, Y.,Kayama, M.,Nishido, H.,Okumura, T.,Yagi, K.,Itaya, T.,Sano, Y. Universitetsforlaget ; Elsevier Science Ltd 2012 Lithos Vol.134 No.-

The timing of partial melting and the pressure-temperature (P-T) paths in the High Himalayan Crystalline Sequence (HHCS) in far-eastern Nepal has been investigated using zircon chronology, rare earth element (REE) compositions, and P-T pseudosection analysis. Zircon from migmatites formed during Himalayan thermal events displays inherited magmatic core overgrown by two generations of metamorphic rims. The new rims are distinguished on the basis of their Tertiary ages, low MREE contents, and low Th/U ratios. The inner zircon rims from Sil+Grt+Bt+Kfs+Pl+Qtz and Ky+Sil+Grt+Bt+Ms+Pl+Qtz migmatites at different structural level of the HHCS display ages of c. 33-28Ma (Early Oligocene) and c. 21-18Ma (Early Miocene): these rims are characterized by flat MREE to HREE patterns and were overgrown by partial melt through muscovite dehydration melting under the stability of garnet, which occurred at P=c. 7-10kbar and T=c. 730-780<SUP>o</SUP>C, and at P=c. 8-14kbar and T=c. 720-770<SUP>o</SUP>C, respectively. The outer zircon rims are relatively enriched in HREE with respect to the inner rims and were overgrown at c. 27-23Ma (Late Oligocene) and at c. 18-16Ma (Early Miocene) during melt crystallization accompanying breakdown of garnet at P=c. 4-7kbar and T=c. 650-725<SUP>o</SUP>C. Early Miocene Ms-Bt leucogranites with two successively overgrown zircon rims at c. 18.3+/-0.3Ma and c. 16.3+/-0.2Ma were intruded into Early Oligocene migmatite hosts. Microstructural observations and the corresponding P-T conditions associated with the two generations of zircon rims indicate that the Early Oligocene and Early Miocene migmatites show relatively isobaric and nearly isothermal P-T paths during exhumation, respectively. The inferences are consistent with higher average cooling rates for the Early Miocene (c. 30-40<SUP>o</SUP>C/My) than the Early Oligocene (c. 15-25<SUP>o</SUP>C/My) migmatites, inferred from peak-T conditions and FT (c. 6Ma for both migmatites) and U-Pb zircon ages. The P-T-t paths of the two migmatites indicate that burial of the Early Miocene migmatites has been coeval with exhumation of the Early Oligocene migmatites, implying the formation of large-scale thrust within the HHCS.

Deletion in HSP110 T₁₇: correlation with wild-type HSP110 expression and prognostic significance in microsatellite-unstable advanced gastric cancers

Kim, K.J.,Lee, T.H.,Kim, J.H.,Cho, N.Y.,Kim, W.H.,Kang, G.H. W. B. Saunders Co ; Centrum Philadelphia 2017 Human pathology Vol.67 No.-

<P>Deletion of the HSP110 T-17 mononucleotide repeat has recently been identified as a prognostic marker that is correlated with wild-type HSP110 (HSP110wt) expression in microsatellite instability-high (MSI-H) colorectal cancers. The aim of this study was to assess the correlation between deletion of the HSP110 T-17 repeat and expression of HSP110wt using DNA testing and immunohistochemistry and to determine the prognostic implications of HSP110 T-17 deletion in MSI-H advanced gastric cancers (GCs). The status of HSP110wt expression was evaluated by immunohistochemistry using an HSP110wt-specific antibody in 142 MSI-H advanced GCs. The size of the HSP110 T-17 repeat deletion was analyzed in 96 MSI-H advanced GCs; deletions were divided into small (0-2 base pairs) and large deletions (3-5 base pairs). Low and high expressions of HSP110wt were detected in 38 (26.8%) and 104 (73.2%) of the 142 cases, respectively. The HSP110 T-17 deletion was observed in 45 (46.9%) of the 96 MSI-H GC samples. Tumors with high expression of HSP110wt showed a tendency to have small or no deletion of HSP110 T-17. In Kaplan-Meier survival analysis, tumors with a large HSP110 T-17 deletion were associated with favorable overall survival and disease-free survival compared with those with small/no deletion of HSP110 T-17. However, HSP110 T-17 deletion size was not an independent prognostic factor in multivariate analysis. In summary, deletion of the HSP110 T-17 repeat was frequently observed in MSI-H GCs, and HSP110 T-17 deletion size was inversely correlated with HSP110wt expression status. Large HSP110 T-17 was not a prognostic indicator in MSI-H GCs. (C) 2017 Elsevier Inc. All rights reserved.</P>

고단백질 사료 급여시 사료내 에너지 , 단백질 수준과 사료의 가공방법이 이스라엘 잉어의 성장 , 소화율 및 오염부하량에 미치는 영향

원태희(T . H . Won),한인규(I . K . Han),주광석(K . S . Chu) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.6

PCR - RFLP 기법을 이용해 젖소개량을 위한 유전적 표지로서 K- Casein 좌위의 유전자형 분석

정의룡(E . R . Chung),김우태(W . T . Kim),최석호(S . H . Choi),임태진(T . J . Rhim),한상기(S . K . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.1

Genotypes of K-casein(K-CN) locus as a genetic marker linked to quantitative trait loci affecting traits of economic importance in dairy cattle were determined by PCR-RFLP method. Genomic DNA was prepared from blood of Holstein cows. The PCR was used to amplify an 874 by region between nucleotides 10592 and 11466 from exon IV to intron IV of the bovine K-CN gene using sense primer(5`-GTGCTGAGTAGGTATCCTAG-3`) and antisense primer(5`GTAGAGTGCAACAACACTGG-3`). After amplification, PCR products were digested with four restriction enzymes, Hind III, Rsa I, Taq I, and Pst I, and the fragments were separated by agarose gel electrophoresis for RFLP analysis of K-CN locus. In addition to screening for the known Hind III and Rsa I restriction site polymorphisms of K-CN locus, we have found additional RFLPs specific for the K-CN A and B alleles in Taca I and Pst I enzymes. The amplified DNA product digested with each restriction enzyme generated specific RFLP pattern that allowed precise identification of K-CN AA, BB or AB genotypes. The K-CN genotypes determined for cows by the PCR-RFLP method agreed completely with the phenotypes obtained from milk samples of the same individuals. Thus, PCR amplification and RFLP analysis was shown to be a rapid and sensitive method for the discrimination of K-CN genotypes directly at the DNA level in dairy cattle of any age or sex. Consequently, the PCR-RFLP method presented in this study can be used as a valuable tool for early selection of AI bulls and calves with desirable K-CN B gene or K-CN BB genotype affecting superior milk production traits for genetic improvement of Holstein dairy cattle.

Growth Performance and Carcass Characteristics of Korean Native Ducks Fed Diets with Varying Levels of Limiting Amino Acids

Choo, Y.K.,Kwon, H.J.,Oh, S.T.,Kang, C.W.,Kim, H.K.,Hong, E.C.,Heo, K.N.,Lee, S.K.,An, B.K. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

There are multiple experiments conducted with male Korean native ducks (KND) to evaluate the optimal levels of limiting amino acids (AA). In Exp. 1, a total of 450 one-d-old male KNDs were divided into five groups with six replicates and fed experimental diets with varying levels of lysine, total sulfur amino acids (TSAA) and threonine (T1, 0.90/0.74/0.70%; T2, 1.00/0.82/0.77%; T3, 1.10/0.90/0.85%; T4, 1.20/0.98/0.93%; T5, 1.30/1.07/1.01%) to 21 d of age. In Exp. 2, one-d-old male KND were received and fed commercial starter diet from hatching to 21 d of age, and then divided into five groups with six replicates and fed one of five diets varying levels of lysine, TSAA, and threonine (T1, 0.73/0.62/0.54%; T2, 0.80/0.68/0.60%; T3, 0.87/0.74/0.65%; T4, 0.94/0.80/0.70%; T5, 1.01/0.86/0.75%) during 22 to 56 d of age, respectively. The BW gain was linearly increased as dietary limiting AA levels increased to 1.20% lysine, 0.98% TSAA and 0.93% threonine. There were no significant differences in feed intake, gain:feed and uniformity among groups. In Exp. 2, the BW gain and gain:feed were not affected by dietary limiting AA levels. There were no significant differences in carcass characteristics and meat quality among groups. The growth performance and carcass characteristics did not show the significant response to increasing dietary limiting AA levels in KND during 22 to 56 d of age. In conclusion, the levels of lysine, TSAA and threonine necessary to maximize growth for starter phase were at least 1.20%, 0.98%, and 0.93%, respectively. On the other hands, KND require relatively low levels of limiting AA for late growth and carcass yield. The dietary levels of 0.73% lysine, 0.62% TSAA and 0.54% threonine appear to be adequate during growing phase.

한우 사골, 꼬리, 우족 및 잡뼈 추출물의 이화학적 특성

이진규(J. K. Lee),정지택(J. T. Jeong),최정석(J. S. Choi1),정명옥(M. O. Jung),최양일(Y. I. Choi) 충북대학교 동물생명과학연구소 2017 동물생명과학연구 Vol.9 No.-

This study was conducted to investigate the physico-chemical characteristics of various bone extracts in Hanwoo cattle. The experimental materials are as follows; T1(Shank bone extract), T2(Tail extract), T3(Beef feet extract), and T4(The other bones extract). In the proximate composition, the T1 and T2 showed higher moisture and fat contents than those of T3 and T4(p<0.05), and the T3 had the highest protein content among the bone extracts(p<0.05). The quality properties including salinity, sugar content, turbidity and yellowness(b*) values were significantly higher in the T3 than the other bone extracts(p<0.05). However, the pH value of T3 was significantly lower than the other bone extracts. In the mineral contents, calcium contents of bone extracts were 36.33-48.57mg/kg, and the content of phosphorus was the lowest in T1(4.62mg/kg) and T2(19.88mg/kg) was the highest(p<0.05). Contents of sodium and potassium were the highest in T3(215.83mg/kg) and T2(54.59mg/kg), while the content of magnesium was the lowest in T3(12.28mg/kg). In conclusion, these results could be suggested to be the basis information for processed meat extract products industry in korea using Hanwoo bone extract.

Study of B^+/-→K^+/-(K_SKπ)⁰ decay and determination of η_c and η_c(2S) parameters

Belle Collaboration,Vinokurova, A.,Kuzmin, A.,Eidelman, S.,Arinstein, K.,Aulchenko, V.,Aushev, T.,Bakich, A.M.,Balagura, V.,Barberio, E.,Belous, K.,Bhardwaj, V.,Bondar, A.,Bozek, A.,Bracko, M.,Brodzic North-Holland Pub. Co 2011 Physics letters: B Vol.706 No.2

We report the results of a study of B<SUP>+/-</SUP>→K<SUP>+/-</SUP>η<SUB>c</SUB> and B<SUP>+/-</SUP>→K<SUP>+/-</SUP>η<SUB>c</SUB>(2S) decays followed by η<SUB>c</SUB> and η<SUB>c</SUB>(2S) decays to (K<SUB>S</SUB>Kπ)<SUP>0</SUP>. The results are obtained from a data sample containing 535 million BB@?-meson pairs collected by the Belle experiment at the KEKB e<SUP>+</SUP>e<SUP>-</SUP> collider. We measure the products of the branching fractions B(B<SUP>+/-</SUP>→K<SUP>+/-</SUP>η<SUB>c</SUB>)B(η<SUB>c</SUB>→K<SUB>S</SUB>K<SUP>+/-</SUP>π<SUP>@?</SUP>)=(26.7+/-1.4(stat)<SUB>-2.6</SUB><SUP>+2.9</SUP>(syst)+/-4.9(model))x10<SUP>-6</SUP> and B(B<SUP>+/-</SUP>→K<SUP>+/-</SUP>η<SUB>c</SUB>(2S))B(η<SUB>c</SUB>(2S)→K<SUB>S</SUB>K<SUP>+/-</SUP>π<SUP>@?</SUP>)=(3.4<SUB>-1.5</SUB><SUP>+2.2</SUP>(stat+model)<SUB>-0.4</SUB><SUP>+0.5</SUP>(syst))x10<SUP>-6</SUP>. Interference with the non-resonant component leads to significant model uncertainty in the measurement of these product branching fractions. Our analysis accounts for this interference and allows the model uncertainty to be reduced. We also obtain the following charmonia masses and widths: M(η<SUB>c</SUB>)=(2985.4+/-1.5(stat)<SUB>-2.0</SUB><SUP>+0.5</SUP>(syst)) MeV/c<SUP>2</SUP>, Γ(η<SUB>c</SUB>)=(35.1+/-3.1(stat)<SUB>-1.6</SUB><SUP>+1.0</SUP>(syst)) MeV/c<SUP>2</SUP>, M(η<SUB>c</SUB>(2S))=(3636.1<SUB>-4.2</SUB><SUP>+3.9</SUP>(stat+model)<SUB>-2.0</SUB><SUP>+0.7</SUP>(syst)) MeV/c<SUP>2</SUP>, Γ(η<SUB>c</SUB>(2S))=(6.6<SUB>-5.1</SUB><SUP>+8.4</SUP>(stat+model)<SUB>-0.9</SUB><SUP>+2.6</SUP>(syst)) MeV/c<SUP>2</SUP>.