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Non-tubular bonded joint under torsion: Theory and numerical validation
Pugno, Nicola,Surace, Giuseppe Techno-Press 2000 Structural Engineering and Mechanics, An Int'l Jou Vol.10 No.2
The paper analyzes the problem of torsion in an adhesive non-tubular bonded single-lap joint. The joint considered consists of two thin rectangular section beams bonded together along a side surface. Assuming the materials involved to be governed by linear elastic laws, equilibrium and compatibility equations were used to arrive at an integro-differential relation whose solution makes it possible to determine torsional moment section by section in the bonded joint between the two beams. This is then used to determine the predominant stress and strain field at the beam-adhesive interface (stress field along the direction perpendicular to the interface plane, equivalent to the applied torsional moment and the corresponding strain field) and the joint's elastic strain (absolute and relative rotations of the bonded beam cross sections). All the relations presented were obtained in closed form. Results obtained theoretically are compared with those given by a three dimensional finite element numerical model. Theoretical and numerical analysis agree satisfactorily.
Dalamon, Viviana,Surace, Ezequiel,Giliberto, Florencia,Ferreiro, Veronica,Fernandez, Cecilia,Szijan, Irene Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.2
Constitutional RB1 gene mutations were studied in a series of 21 families with unilateral and bilateral retinoblastoma patients. Peripheral blood lymphocytes were analyzed by "exon by exon" PCR-heteroduplex and sequencing. Mutations were identified in 6 (29%) of the patients. One mutation corresponded to an intronic polymorphism in g.174351T > A. The other five mutations resulted C to T exonic transitions, four were CGA sequences (g.65386, g.150037 in two patients, and g.162237), creating stop codons and presumably truncated proteins. The fifth one was new and resulted in alanine to valine substitution (g.73774). Two patients had the same the germline truncated mutation (g.150037C > T), one with a familial bilateral early onset retinoblastoma and one with a sporadic unilateral late onset retinoblastoma. The later type has not been previously described. This finding is discussed in the genotype/phenotype correlation context. Additionally, a single nucleotide change was found in six studied samples, where a C to T homozygous transversion was identified in intron 26 (IVS26 + 28). It is worthy the non concordance of the nucleotide with the published sequence. This analysis proved to be a useful method for the detection of mutations in the RB1 gene, and contributed to the adequate genetic counseling to patients and relatives.
Influence of sharp stiffness variations in damage evaluation using POD and GSM
Thiene, M.,Galvanetto, U.,Surace, C. Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.14 No.4
Damage detection methods based on modal analysis have been widely studied in recent years. However the calculation of mode shapes in real structures can be time consuming and often requires dedicated software programmes. In the present paper the combined application of proper orthogonal decomposition and gapped smoothing method to structural damage detection is presented. The first is used to calculate the dynamic shapes of a damaged structural element using only the time response of the system while the second is used to derive a reference baseline to which compare the data coming from the damaged structure. Experimental verification is provided for a beam case while numerical analyses are conducted on plates. The introduction of a stiffener on a plate is investigated and a method to distinguish its influence from that of a defect is presented. Results highlight that the derivatives of the proper orthogonal modes are more effective damage indices than the modes themselves and that they can be used in damage detection when only data from the damaged structure are available. Furthermore the stiffened plate case shows how the simple use of the curvature is not sufficient when analysing complex components. The combined application of the two techniques provides a possible improvement in damage detection of typical aeronautical structures.
( Viviana Dalamon ),( Ezequiel Surace ),( Florencia Giliberto ),( Veronica Ferreiro ),( Cecilia Fernandez ),( Irene Szijan ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.2
Constitutional RB1 gene mutations were studied in a series of 21 families with unilateral and bilateral retinoblastoma patients. Peripheral blood lymphocytes were analyzed by exon by exon PCR-heteroduplex and sequencing. Mutations were identified in 6 (29%) of the patients. One mutation corresponded to an intronic polymorphism in g.174351T>A. The other five mutations resulted C to T exonic transitions, four were CGA sequences (g.65386, g.150037 in two patients, and g.162237), creating stop codons and presumably truncated proteins. The fifth one was new and resulted in alanine to valine substitution (g.73774). Two patients had the same the germline truncated mutation (g.150037C>T), one with a familial bilateral early onset retinoblastoma and one with a sporadic unilateral late onset retinoblastoma. The later type has not been previously described. This finding is discussed in the genotype/phenotype correlation context. Additionally, a single nucleotide change was found in six studied samples, where a C to T homozygous transversion was identified in intron 26(IVS26+28). It is worthy the non concordance of the nucleotide with the published sequence, This analysis proved to be a useful method for the detection of mutations in the RB1 gene, and contributed to the adequate genetic counseling to patients and relatives.
Influence of sharp stiffness variations in damage evaluation using POD and GSM
M. Thiene,U. Galvanetto,C. Surace 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.4
Damage detection methods based on modal analysis have been widely studied in recent years. However the calculation of mode shapes in real structures can be time consuming and often requiresdedicated software programmes. In the present paper the combined application of proper orthogonaldecomposition and gapped smoothing method to structural damage detection is presented. The first is usedto calculate the dynamic shapes of a damaged structural element using only the time response of the systemwhile the second is used to derive a reference baseline to which compare the data coming from the damagedstructure. Experimental verification is provided for a beam case while numerical analyses are conducted onplates. The introduction of a stiffener on a plate is investigated and a method to distinguish its influence fromthat of a defect is presented. Results highlight that the derivatives of the proper orthogonal modes are moreeffective damage indices than the modes themselves and that they can be used in damage detection whenonly data from the damaged structure are available. Furthermore the stiffened plate case shows how thesimple use of the curvature is not sufficient when analysing complex components. The combined applicationof the two techniques provides a possible improvement in damage detection of typical aeronauticalstructures.
Giliberto, Florencia,Ferreiro, Veronica,Dalamon, Viviana,Surace, Ezequiel,Cotignola, Javier,Esperante, Sebastian,Borelina, Daniel,Baranzini, Sergio,Szijan, Irene Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.2
Duchenne muscular dystrophy (DMD) is the most common hereditary neuromuscular disease. It is inherited manifestations. In some rare cases, the disease can also be manifested in females. The aim of the present study was to determine the molecular alteration in two cases of nonrelated DMD symptomatic carriers with no previous history of DMD. Multiplex PCR is commonly used to search for deletion in the DMD gene of affected males. This method could not be used in females because the normal X chromosome masks the deletion of the mutated one. Therefor, we used a set of seven highly polymorphic dinucleotide $(CA)_n$ repeat markers that lie within the human dystrophin gene. The deletions were evidenced by hemizygosity of the loci under study. We localized a deletion in the locus 7A (intron 7) on the maternal X chromosome in one case, and a deletion in the region of introns 49 and 50 on the paternal X chromosome in the other. The use of microsatellite genotyping within the DMD gene enables the detection of the mutant allele in female carriers. It is also a useful method to provide DMD families with more accurate genetic counseling.
A novel approach to damage localisation based on bispectral analysis and neural network
M. Civera,L. Zanotti Fragonara,C. Surace 국제구조공학회 2017 Smart Structures and Systems, An International Jou Vol.20 No.6
The normalised version of bispectrum, the so-called bicoherence, has often proved a reliable method of damage detection on engineering applications. Indeed, higher-order spectral analysis (HOSA) has the advantage of being able to detect non-linearity in the structural dynamic response while being insensitive to ambient vibrations. Skewness in the response may be easily spotted and related to damage conditions, as the majority of common faults and cracks shows bilinear effects. The present study tries to extend the application of HOSA to damage localisation, resorting to a neural network based classification algorithm. In order to validate the approach, a non-linear finite element model of a 4-meters-long cantilever beam has been built. This model could be seen as a first generic concept of more complex structural systems, such as aircraft wings, wind turbine blades, etc. The main aim of the study is to train a Neural Network (NN) able to classify different damage locations, when fed with bispectra. These are computed using the dynamic response of the FE nonlinear model to random noise excitation.
( Florencia Giliberto ),( Veronica Ferreiro ),( Viviana Dalamon ),( Ezequiel Surace ),( Javier Cotignola ),( Sebastian Esperante ),( Daniel Borelina ),( Sergio Baranzini ),( Irene Szijan ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.2
Duchenne muscular dystrophy (DMD) is the most common hereditary neuromuscular disease. It is inherited as an X-linked recessive trait in which males show clinical manifestations. In some rare cases, the disease can also be manifested in females. The aim of the present study was to determine the molecular alteration in two cases of non-related DMD symptomatic carriers with no previous history of DMD. Multiplex PCR is commonly used to search for deletion in the DMD gene of affected males. This method could not be used in females because the normal X chromosome masks the deletion of the mutated one. Therefore, we used a set of seven highly polymorphic dinucleotide (CA), repeat markers that lie within the human dystrophin gene. The deletions were evidenced by hemizygosity of the loci under study. We localized a deletion in the locus 7A (intron 7) on the maternal X chromosome in one case, and a deletion in the region of introns 49 and 50 on the paternal X chromosome in the other. The use of microsatellite genotyping within the DMD gene enables the detection of the mutant allele in female carriers. It is also a useful method to provide DMD families with more accurate genetic counseling.