Identification of a Transferrin Receptor-binding Peptide from a Phage-displayed Peptide Library

Sungil Kim(김성일),Suk-Jung Choi(최석정) 한국생명과학회 2008 생명과학회지 Vol.18 No.3

펩타이드 문고 기술을 이용하여 흑색종 세포주인 B16F10에 결합하는 펩타이드 리간드를 검색하였다. 먼저 세포 내부로 들어간 파지들을 선택하는 방법으로 두 번 검색한 후 표면에 결합한 파지들 가운데 트랜스페린 단백질을 이용하여 트랜스페린 수용체에 결합한 파지들만을 선별적으로 용출시키는 방법으로 세 번 검색하였다. 다음으로 이 두 가지 방법을 통해 선별된 파지들에 표현된 펩타이드들을 Pseudomonas exotoxin의 전이 영역과 촉매 영역에 융합시킨 재조합 독소들을 만들었다. B16F10 세포에 대한 각 재조합 독소의 활성을 측정하여 일곱 개의 클론을 선택한 후 염기서열을 분석하였다. 그 결과 그 가운데 한 클론에서 표현하는 펩타이드의 아미노산 서열이 사람의 트랜스페린과 유사한 서열을 갖는 것으로 확인되었다. 그 펩타이드를 화학적으로 합성한 후 항암제를 포함하는 리포솜에 붙여 실험한 결과 트랜스페린 수용체를 통해 치료물질을 전달할 수 있는 가능성을 지닌 것으로 평가되었다. Using a phage peptide library approach, we have isolated a peptide ligand that binds to transferrin receptor on the surface of human melanoma cell, B16F10. The library was first screened twice by recovering internalized phages and was further screened three times by competitively eluting transferrin receptor-specific phages with human transferrin among the phages bound to the cell surface. The peptides displayed by the selected phages were fused to translocation and catalytic domain of Pseudomonas exotoxin to prepare recombinant toxins. After estimating cytotoxicity of each recombinant toxin toward B16F10 cell, seven clones were selected. Sequence analysis revealed that one of the clones displayed a peptide which had a significant sequence homology with human transferrin. The peptide was chemically synthesized and was shown to be functional in delivering cytotoxic agents into B16F10 cell via interaction with transferrin receptor.

Demonstration of re-mineralizing RO permeate and utilizing available water resources on a small island in Korea for drinking water supply

Sungil Lim(임성일),Yu Chang Kim(김유창) 대한환경공학회 2022 대한환경공학회 학술발표논문집 Vol.2022 No.11

Using CAD Systems for LED Placement and to Generate Electric Circuits for Smart Glass

Sungil Ham(함성일) (사)한국CDE학회 2022 한국CDE학회 논문집 Vol.27 No.2

Industrial companies aim to improve their productivity by using the latest technologies such as Big Data, artificial intelligence, and IoT. However, small and medium-sized companies (SMEs) often lag behind with the introduction of new technologies and refrain from automating their production processes. In this regard, SMEs that produce smart glass, the production of which requires advanced technology, often continue to depend on manual production processes. We analyzed the existing production process to determine whether the integration of CAD technologies could be used to improve the productivity. This study was limited specifically to the development of algorithms for placing the LEDs in the glass in correspondence with the designed shape and to automatically generate a circuit for delivering current to the LEDs. The arrangement of the LEDs is designed to minimize the discrete Fréchet distance in consideration of the “local maximum point of curvature” and “kink point,” and the circuit is automatically organized by grouping a certain number of LEDs to supply similar current to the LEDs. Finally, the time required for LED placement and circuit preparation was reduced from 2 hours to 10 minutes.

Teaching L2 Vocabulary Knowledge

( Sungil Lee ) 한국중등영어교육학회 2010 중등영어교육 Vol.3 No.2

L2 learners of English need to learn a large amount of vocabulary to read authentic texts and to communicate in the target language. Schmitt (2008) suggests that L2 learners are required to know 2000-3000 word families to understand spoken English, and 6000-7000 word families to cover 98%of texts. Because of the limited time for which L2 learners are exposed to the target language, to gain the enough amount of vocabulary is not a piece of cake. Along with broadening vocabulary size, L2 learners are required to deepen their vocabulary knowledge. Vocabulary knowledge includes the different aspects of word knowledge such as grammatical features, word associations, register constraints, syntactic features, and so on. In this regard, how effectively should vocabulary be taught and tested has been the center of the research of vocabulary acquisition. In the present paper, several issues in learning L2 vocabulary knowledge and pedagogy are mentioned.

Regional Difference and Counterfactual Decomposition of Pro-Poor Growth:

Sungil Kwak 대외경제정책연구원 2011 Working Papers Vol.2011 No.1

대부분의 선행연구들은 지역별 이질성의 존재를 무시한 상태에서 성장, 불평등, 그리고 빈곤 간의 상호관계에 관해 조사하였다. 본 연구에서는 에티오피아의 농촌지역을 생산기술과 기후의 차이에 근거해서 세 지역(highland, hoe, enset)으로 나누어 성장과 빈곤의 관계를 분석했다. 먼저 지역별 이질성이 존재함을 보인 후에, 의사고정효과 프로빗 모형(Pseudo-fixed effect Probit model)을 추정하여 세 지역의 빈곤결정인자가 다름을 보였다. 빈곤한 가구는 자산 증가, 또는 그 자산의 수익률 증가가 발생하여 행복감(well-being)의 개선이 기대될 때 빈곤에서 벗어날 수 있다. 따라서 우리는 역사실 분해(counterfactual decomposition)를 사용하여, 빈곤을 줄이는 성장(Pro-poor growth)을 가구가 보유한 관찰 가능한 자산량의 변화와 그 자산의생산성 변화로 분해하였다. 호(hoe) 지역에서는 생산성의 변화가 빈곤을 줄이는 성장에 유의한 영향을 주는 것으로 나타난 반면에, 다른 두 지역(highland 지역과 enset 지역)에서는 유의하게 나타나지 않았다. 성장의 성격 또한 지역에 따라 이질적으로 나타났다. 상대적인 의미에서 빈곤을 줄이는 성장이 호 지역에서 나타났는데, 이는 생산성의 변화가 성장에 미치는 영향이 소득분포를 따라 이질적으로 나타났기때문이다. 반대로, 다른 두 지역에서는 생산성의 변화가 성장에 미치는 영향이 상대적의미에서 빈곤을 줄이는 성향을 나타내지 않았다. 따라서 생산성의 변화가 가구의 성장에 미치는 영향이 지역에 따라 다르게 나타나므로, 빈곤한 가구의 생산성을 늘리기 위해 보급되는 생산기술이 빈곤을 줄이는 성장을 가져올 수 있을지에 관해 각수원지역의 환경에 기초해서 검증한 후, 그 지식과 기술을 보급해야 할 것이다. 예를 들어 수원국에 해외농업기술개발(Korea Project on International Agriculture) 센터를 건립하여 농업기술개발 협력사업을 추진할 때나 지식공유사업(KSP)을 통해 새로운 지식을 보급할 때, 이 같은 지식과 기술보급이 빈곤을 줄이는 성장 전략인지 검증할 필요가 있다. Previous literature, ignoring regional heterogeneity, has mainly explored the interrelationship among growth, inequality, and poverty. In exploring the incidence of poverty and growth, we classify rural Ethiopia into three regions based on the difference of production technologies and climates. We find evidence that regional heterogeneity exists across the three regions. To find the sources of heterogeneity, we estimate a pseudo-fixed effect probit model controlling for household fixed effects within a random effect probit model across regions. We find that poverty is determined by different sources across the three regions, each with different farming systems. Moreover, poor households can escape poverty only when their expected level of well-being has been improved by increases in asset holdings and/or returns to assets. Hence, we propose, using counterfactual decomposition, that propoor growth can be decomposed into two components: changes in the amount of attributes such as observable household assets or capital, and changes in ‘aggregate marginal product’ of the attributes. We find that the impacts of the changes in the aggregate marginal product on pro-poor growth are significant in the hoe area, but the changes in attributes do not significantly affect growth in this region. The aspects of growth are determined heterogeneously across regions: in the highland area, both components work together; in the hoe area, growth is mainly determined by changes in the aggregate marginal product; and in the enset area, it is changes in attributes that mainly determine the positive growth. We find evidence that pro-poor growth in a relative sense appear in the hoe area, where the impact of changes in the aggregate marginal product on growth is heterogeneous along the income distribution; the larger impact appears in the lower tails, while smaller impact can been seen in the upper tails. However, we find no evidence of pro-poor growth in the highland and the enset areas, where the impacts of the aggregate marginal product on growth are anti-poor and insignificant, respectively. Therefore, since the impact of changes in productivity on growth differs across regions, technology disseminated to increase household productivity should be tested whether it could generate pro-poor growth in the recipient’s environment. For example, in providing agricultural technologies through the Korea Project on International Agriculture (KOPIA) or new knowledge via the Knowledge Sharing Program (KSP), we have to deliberate carefully on whether the knowledge and technology do indeed have propoor aspects.

Financial Inclusion and Microfinance:

Sungil Kwak,Ju Young Lee 대외경제정책연구원 2014 World Economy Brief Vol.2014 No.01

The Challenges of Korean Development NGOs and Their Comparative Advantages

Sungil Kwak 대외경제정책연구원(KIEP) 2013 KIEP Opinions Vol.2013 No.11

Korea's Cooperation Schemes to Development of Small and Medium Enterpriese in Ethiopia

Sungil Kwak,Hyelin Jeon,Seukhoon Baik 대외경제정책연구원 2013 World Economy Brief Vol.2013 No.01

Outer-selective Hollow Fiber Thin-film Composite Membranes for Forward Osmosis Applications

Sungil Lim,Sherub Phuntsho,Ho Kyong Shon 한국막학회 2020 한국막학회 총회 및 학술발표회 Vol.2020 No.11

Complementarity of Horizontal and Vertical Multinational Activities

Sungil Bae,Tae Hwan Yoo 한국무역학회 2005 Journal of Korea trade Vol.9 No.2

This paper explores the multinational activities of the Japanese automobile industry by focusing on the vertical production structure of a firm. The main objective of this paper is to analyze the effects of horizontal multinational activities on vertical multinational activities. First, we construct a theoretical model to predict whether an increase in horizontal multinational activity leads to an increase in existing vertical multinational activity. This result mainly comes from the increased flow of intermediate products. Second, we test a theoretical model by applying annual data from Japanese automobile industry for 1982 to 1997. The estimated results provide a complementary relationship between horizontal and vertical multinational activities, thereby supporting the results of the theoretical model.