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골프장의 잔디 토양에서 살균제 Tolclofosmethyl의 분해
정근욱,우선희,김흥태,사동민,김영기,홍순달,김재정,이재구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3
Tolclofosmethyl (TCFM) is heavily and annually applied to the turf soils of most golf courses in Gyeongju to control the fungi known to cause the disease brown patch. The soil samples used for the experiment was collected three weeks after the annual application at the end of May in the year 2002. The preliminary results obtained from this study demonstrated that the repeated field annual applications of TCFM to the turf soils of a golf course located in Gyeongju city in the southern area of Korea showed the enhanced degradation of the parent compound TCFM, especially in the surface (0~15 cm) soil rather than the shallow subsurface (15~30 cm) and deep subsurface (30~45 cm) soils, compared to the corresponding surface (0~15 cm) and shallow and deep subsurface (15~30 cm and 30~45 cm) soils of the untreated plot. It appears that microorganisms in the soil may be involved in the enhanced degradation of TCFM.
Prediction of Rice Embryo Proteins using EST-Databases
Sun-Hee Woo,Seung-Woo Cho,Tae-Seon Kim,Keun-Yook Chung,Yong-Gu Cho,Hong-Sig Kim,Beom-Heon Song,Chul-Won Lee,Seung-Keun Jong 한국육종학회 2008 한국육종학회지 Vol.40 No.1
An attempt was made to link rice embryo proteins to DNA sequences and to understand their functions. One hundred of the 700 spots detected on the embryo 2-DE gels were microsequenced. Of these, 28% of the embryo proteins were matched to DNA sequences with known functions, but 72% of the proteins were unknown in functions as previously reported (Woo et al. 2002). In addition, twenty-four protein spots with 100% of homology and nine with over 80% were matched to ESTs (expressed sequence tags) after expanding the amino acid sequences of the protein spots by Database searches using the available rice EST databases at the NCBI (http://www/ncbi.nlm.nih.gov/) and DDBJ (http://www.ddbj.nig.ac.jp/). The chromosomal location of some proteins were also obtained from the rice genetic map provided by Japanese Rice Genome Research Program (http://rgp.dna.affrc.go.jp). The DNA sequence databases including EST have been reported for rice (Oryza sativa L.) now provides whole or partial gene sequence, and recent advances in protein characterization allow the linking proteins to DNA sequences in the functional analysis. This work shows that proteome analysis could be a useful tool strategy to link sequence information and to functional genomics.
Hee-Young Jang,Soo Jong Kwon,Ki-Hyun Kim,Keun-Yook Chung,Yong-Gu Cho,Jong-Soon Choi,YoungMok Park,Setsuko Komatsu,Sun-Hee Woo 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Recently, proteome analysis is becoming a powerful tool for the functional characterization of plants. Due to the availability of vast nucleotide sequence information and based on the progress achieved in sensitive and rapid protein identification by mass spectrometry, proteome approaches open up now perspectives to analyze the complex functions of model crop species at different level. In this study, we have N-terminal sequencing data for the 100 embryo and 53 seed proteins of rice separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were collected and systematically organized for a protein sequence data-file. An attempt was made to link the embryo proteins of rice to DNA sequences for understanding their functions. One hundred proteins of the 700 spots were detected in the embryo using 2-DE gels whereas we used micro sequenced. Of these, 28% of the embryo proteins were matched to DNA sequences with known functions, but 72% of the proteins were identified to be unknown functions as previously reported by Woo et al.,. In addition, twenty-four spots of protein with 100% of homology and nine with over 80% were matched to ESTs (expressed sequence tags) after expanding the amino acid sequences of the protein spots by Database searches using the available EST databases of rice at the NCBI (http://www/ncbi.nlm.nih.gov/) and DDBJ (http://www.ddbj.nig.ac.jp/). Also, a total of 53 proteins out of 700 protein spots separated on the 2-DE gels were analyzed by the peptide mass fingerprinting method (MALDI-TOF/MS). High-quality mass spectra suitable for peptide mass fingerprinting were obtained from 41 spots. Using the ESI-Q-TOF/MS, however, we were able to identify 53 seed proteins of rice, including 12 proteins not registered in database. The rapid expansion of DNA sequence databases to the utilization of EST now provides the whole or partial gene sequences of model organisms, and the recent advances in protein micro-characterization by mass spectrometry allow the possibility of linking these DNA sequences to the proteins in functional complexes. Proteome Database of rice is updated, and is available on the World Wide Web at http://gene64.rda.affrc.go. This work shows that the proteome analysis could be a useful strategy to link the sequence information to the functional genomics.
Pollen-tube Behavior and Embryo Development in Interspecific Crosses Among the Genus Fagopyrum
( Sun Hee Woo ),( Soo Hwan Kim ),( Kei Sei Tsai ),( Keun Yook Chung ),( Seung Keun Jong ),( Taiji Adachi ),( Jong Soon Choi ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.4
Common buckwheat (Fagopyrum esculentum Moench) is an agriculturally and pharmaceutically valuable crop due to its wellbalanced essential amino acids and rutin content. However, global mass production of buckwheat is limited because its genetic self-incompatibility results in low seed sets and poor grain yield. Therefore, this study was conducted to classify the modes of pistil-pollen interaction between species belonging to the genus Fagopyrum and to determine the optimal combination of outcrosses for the most successful pollinations. Based on the interaction between pistils and pollen, we classified the modes of pollen tube growth during interspecific crosses of Fagopyrum species into five categories: (i) Highly compatible: normal pollen tube elongation and style penetration within 6~24 hours of pollination, (ii) Slightly compatible: delayed (for 1~6 hours) pollen tube elongation and normal style penetration, (iii) Incompatible type I: pollen tube inhibition at the stigma, (iv) Incompatible type II: pollen tube inhibition at the style, and (v) Incompatible type III: pollen tube inhibition at the stylodium. Based on the observed pollen tube elongation and the following embryo development, highly compatible pollinations were found to be crosses between F. esculentum x F. cymosum and between F. esculentum (thrum) x F. homotropicum.