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Sulaiman F. A.,Iyiola O. A.,Anifowoshe T. A.,Sulaiman A. A.,Bello O. K.,Akinyele T. J.,Jimoh A. M.,Maimako R. F.,Otohinoyi D. A.,Osemwegie O. O.,Adeyemi O. S. 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.1
The Trypanosoma brucei is the causative agent of African trypanosomiasis, a disease that affects both humans and animals. Chemotherapy which forms the major means of control for the disease has several shortcomings such as limited efficacy and adverse side effects amongst others. Thus, motivating the search for better therapies. In this study, extracts of some tropical plants including the Acacia nilotica, Bombax buonopozense and Khaya senegalensis were evaluated for therapeutic and prophylactic potential in mouse model of experimental trypanosomiasis. Both diminazene aceturate and isometamidium chloride were included as reference drugs. Results showed that T. brucei caused an elevation in rat plasma indirect bilirubin and a reduction in rat plasma albumin and total protein which suggest mild hepatic dysfunction due to experimental infection. Data also revealed that the plant extracts significantly reduced the rat parasite burden both in the prophylaxis and therapeutic treatment groups when compared with the negative drug control. The infection and treatments had no adverse effect on the rat organ and body weights. The infection did not alter the activity of rat plasma ALT, AST and ALP compared with the administered extracts of A. nilotica and B. buonopozense. Further, the plant extracts ameliorated some trypanosomiasisinduced pathologies in treated rats compared with negative drug control. Taken together, findings do not only lend credence to the folkloric use of these plants for medicinal purposes but also suggest these plant extracts have potential to serve as alternative source of anti-parasitic agents particularly for the control of trypanosomiasis
Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase
Sulaiman, Sintawee,You, Dong-Ju,Kanaya, Eiko,Koga, Yuichi,Kanaya, Shigenori American Chemical Society 2014 Biochemistry Vol.53 No.11
<P>The crystal structure of metagenome-derived LC-cutinase with polyethylene terephthalate (PET)-degrading activity was determined at 1.5 Å resolution. The structure strongly resembles that of <I>Thermobifida alba</I> cutinase. Ser165, Asp210, and His242 form the catalytic triad. Thermal denaturation and guanidine hydrochloride (GdnHCl)-induced unfolding of LC-cutinase were analyzed at pH 8.0 by circular dichroism spectroscopy. The midpoint of the transition of the thermal denaturation curve, <I>T</I><SUB>1/2</SUB>, and that of the GdnHCl-induced unfolding curve, <I>C</I><SUB>m</SUB>, at 30 °C were 86.2 °C and 4.02 M, respectively. The free energy change of unfolding in the absence of GdnHCl, Δ<I>G</I>(H<SUB>2</SUB>O), was 41.8 kJ mol<SUP>–1</SUP> at 30 °C. LC-cutinase unfolded very slowly in GdnHCl with an unfolding rate, <I>k</I><SUB>u</SUB>(H<SUB>2</SUB>O), of 3.28 × 10<SUP>–6</SUP> s<SUP>–1</SUP> at 50 °C. These results indicate that LC-cutinase is a kinetically robust protein. Nevertheless, the optimal temperature for the activity of LC-cutinase toward <I>p</I>-nitrophenyl butyrate (50 °C) was considerably lower than the <I>T</I><SUB>1/2</SUB> value. It increased by 10 °C in the presence of 1% polyethylene glycol (PEG) 1000. It also increased by at least 20 °C when PET was used as a substrate. These results suggest that the active site is protected from a heat-induced local conformational change by binding of PEG or PET. LC-cutinase contains one disulfide bond between Cys275 and Cys292. To examine whether this disulfide bond contributes to the thermodynamic and kinetic stability of LC-cutinase, C275/292A-cutinase without this disulfide bond was constructed. Thermal denaturation studies and equilibrium and kinetic studies of the GdnHCl-induced unfolding of C275/292A-cutinase indicate that this disulfide bond contributes not only to the thermodynamic stability but also to the kinetic stability of LC-cutinase.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2014/bichaw.2014.53.issue-11/bi401561p/production/images/medium/bi-2013-01561p_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bi401561p'>ACS Electronic Supporting Info</A></P>
INTEGRAL INEQUALITY REGARDING γ-CONVEX AND γ-CONCAVE FUNCTIONS
Sulaiman, WaadAllah T. Korean Mathematical Society 2010 대한수학회지 Vol.47 No.2
New integral inequalities concerning $\gamma$-convex and $\gamma$-concave functions are presented.
Sulaiman, Rania S.,Park, Bomina,Sheik Pran Babu, Sardar Pasha,Si, Yubing,Kharwadkar, Rakshin,Mitter, Sayak K.,Lee, Bit,Sun, Wei,Qi, Xiaoping,Boulton, Michael E.,Meroueh, Samy O.,Fei, Xiang,Seo, Seung- American Chemical Society 2018 ACS CHEMICAL BIOLOGY Vol.13 No.1
<P>The standard-of-care therapeutics for the treatment of ocular neovascular diseases like wet age-related macular degeneration (AMD) are biologics targeting vascular endothelial growth factor signaling. There are currently no FDA approved small molecules for treating these blinding eye diseases. Therefore, therapeutic agents with novel mechanisms are critical to complement or combine with existing approaches. Here, we identified soluble epoxide hydrolase (sEH), a key enzyme for epoxy fatty acid metabolism, as a target of an antiangiogenic homoisoflavonoid, SH-11037. SH-11037 inhibits sEH <I>in vitro</I> and <I>in vivo</I> and docks to the substrate binding cleft in the sEH hydrolase domain. sEH levels and activity are up-regulated in the eyes of a choroidal neovascularization (CNV) mouse model. sEH is overexpressed in human wet AMD eyes, suggesting that sEH is relevant to neovascularization. Known sEH inhibitors delivered intraocularly suppressed CNV. Thus, by dissecting a bioactive compound’s mechanism, we identified a new chemotype for sEH inhibition and characterized sEH as a target for blocking the CNV that underlies wet AMD.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/acbcct/2018/acbcct.2018.13.issue-1/acschembio.7b00854/production/images/medium/cb-2017-00854f_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cb7b00854'>ACS Electronic Supporting Info</A></P>
Sulaiman M. Al-Eidi,Ashry Gad Mohamed,Raid A. Abutalib,Abdullah M. AlBedah,Mohamed K.M. Khalil 사단법인약침학회 2019 Journal of Acupuncture & Meridian Studies Vol.12 No.6
To evaluate the feasibility of comparing the effect of the traditional Hijamah and the Asian wet cupping techniques in the management of patients with chronic low back pain (CLBP), a randomized clinical trial comparing traditional and Asian wet cupping techniques for CLBP was conducted in two secondary care hospitals in Saudi Arabia. Seventy eligible participants with CLBP were randomized to receive one session of wet cupping using either Asian technique (34 patients) or traditional Hijamah technique (36 patients). Cupping was performed at four sites of the bilateral bladder meridian (BL23, BL24, and BL25). The numeric rating scale, Present Pain Intensity, and Oswestry Disability Questionnaire scores were measured immediately after intervention, at seven days, and 14 days after intervention. In both groups, there was a significant decrease in the numeric rating scale, Present Pain Intensity, and Oswestry Disability Questionnaire scores, immediately after intervention, at seven days, and 14 days after intervention. However, there was no significant difference between the two groups across all the outcome measures up to 14 days after intervention. The study did not show a superiority of one technique compared with the other. Longer follow-up periods and more than one cupping session may be needed to evaluate the difference, if any, between both the techniques. Trial Registration: NCT02012205.
Current status, challenges, and future prospects of plant genome editing in China
Sulaiman Ahmed,Yandi Zhang,Muhammad Abdullah,Qiuxiang Ma,Hongxia Wang,Peng Zhang 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.5
Genome editing (GE) is the most powerful tool for creating genetic variation in plants. This approach is valuable for studying the mechanism of gene function and regulation as well as to improve desirable traits using sequence-specific endonucleases. It is typically performed with diverse molecular scissors that cleave a particular gene at a defined position. The advent of sequence-specific nucleases such as ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases), and CRISPR (clustered regularly interspaced short palindromic repeats), in particular, have allowed for the precise and efficient introduction of genetic variation into the genome. The newly developed CRISPR-associated protein 9 (Cas9) variants, base-editing systems, novel RNA-directed nucleases, and DNA-free CRISPR/Cas9 delivery methods offer great opportunities for plant genome engineering. China has made tremendous progress in the field of GE for crop improvement to meet the demand of growing population. Herein, we reviewed the recent progress in GE of different crops in China, highlighting advanced GE tools/methods, and also discussed the specific challenges and prospects of plant GE.