Effects of Various Sources and Levels of Chromium on Performance of Broilers

Suksombat, Wisitiporn,Kanchanatawee, S. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11

Three hundred and twenty four one day old mixed sex broiler chicks were assigned at random into 9 treatment groups. The experimental design was a 3${\times}$3 factorial arrangement. During the starter period (week 1-3), chicks were fed ad libitum. A cornsoybean meal based diet contained 23% crude protein, 3,200 kcal/kg metabolizable energy (NRC, 1994), and supplemented with organic or inorganic forms of chromium. Two organic chromium products, chromium yeast (Cr-Yeast from Alltech Biotechnology Corporation Limited) and chromium picolinate (Cr-Pic) were supplemented at the rate of 200, 400 and 800 ppb. One inorganic product, chromium chloride, was supplemented at the rate of 200, 400 and 800 ppb. During the finishing period (week 4-7), the corn-soybean meal based diet contained 20% crude protein, 3,200 kcal/kg metabolizable energy (NRC, 1994), and the same levels of chromium as in the starter period were added. No significant difference was observed among treatment groups in average daily gain, feed intake, body weight gain, feed conversion ratio and mortality. The carcass percentage of broilers receiving 200 and 400 ppb organic chromium (Cr-Yeast or Cr-Pic) was significantly increased (p<0.01). In addition, the supplementation of organic chromium reduced (p<0.05) breast meat fat content but increased breast meat protein content. The addition of chromium in the diet had no effect on boneless breast, skinless boneless breast, boneless leg, skinless boneless leg but reduced percentage of sirloin muscle. Total cholesterol and triglycerides were reduced by organic Cr supplementation. Supplementation with 200 and 400 ppb of both Cr-Yeast and Cr-Pic showed the lowest total cholesterol. The effects of type of Cr on HDL and LDL were variable, however, LDL increased with increasing level of Cr supplementation. This trial indicates that organic chromium tended to improve growth performances and carcass composition, reduced total cholesterol and triglycerides. The optimum level of organic chromium supplementation was at 200 ppb.

Effects of Soybean Oil or Rumen Protected Conjugated Linoleic Acid Supplementation on Accumulation of Conjugated Linoleic Acid in Dairy Cows' Milk

Suksombat, Wisitiporn,Chullanandana, Khukbuan Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.9

The effects of feeding soybean oil (SBO) or rumen protected conjugated linoleic acid (RP-CLA) on CLA accumulation in milk, and performance of lactating dairy cows were studied. Twenty four Holstein Friesian crossbred lactating dairy cows, averaging $126{\pm}45days$ in milk, $15.6{\pm}2.43kg$ of milk and $452{\pm}51kg$ body weight were stratified randomly and assigned in a randomized complete block design (RCBD) to three treatments of 8 cows each. The treatments were control, 150 g of SBO and 150 g of RP-CLA supplementation. Performance parameters showed that DM intake, NELP intake and body weight change were similar across treatments, while CP intake was decreased by SBO and RP-CLA supplementation. Milk yield and milk composition were not significantly different among treatments, except for milk fat percentage and fat yield which were significantly decreased by 27% (p<0.05) and by 28% (p<0.01), respectively, by RP-CLA supplements compared with control treatment. Feeding RP-CLA reduced 3.5% FCM compared with the other treatments (p<0.003). Both SBO and RP-CLA supplementation reduced <C16:0 fatty acids but increased ${\geq}C18:0$ and CLA concentration in milk fat.

Effects of Soybean Oil or Whole Cotton Seed Addition on Accumulation of Conjugated Linoleic Acid in Beef of Fattening Brahman×Tai-Native Catle

Suksombat, Wisitiporn,Chullanandana, Khukhuan Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10

Effects of soybean oil or whole cotton seed addition on conjugated linoleic acid (CLA) and performance of fattening Brahman$\times$Thai-Native cattle were studied. Eighteen fattening cattle averaging $241{\pm}24kg$ body weight and approximately 1 year old were stratified by live weight into three groups and randomly assigned by group to one of three dietary treatments. The treatments were control (concentrated 14% crude protein), control and supplemented with 170 g/d soybean oil, control plus 170 g/d of oil from whole cotton seed. All animals were weighed before and after the experimental period and 4 cattle per treatment were randomly slaughtered then carcass measurements were obtained. There were no statistically significantly differences in the final body weight, average daily gain and dry matter intake among treatments. However, the crude protein intake was significantly decreased (p<0.01) when whole cotton seed was fed compared with control and soybean oil treatments. The carcass composition and carcass characteristics were not significantly different in Longissimus and Semimembranosus muscle by feeding soybean oil and whole cotton seed compared with the control treatment. Supplementation of soybean oil increased (p<0.01) cis-9, trans-11 CLA by 116% in Longissimus muscle and by 240% in Semimembranosus muscle. However, whole cotton seed did not increase cis-9, trans-11 CLA in both muscles. The present study successfully increased cis-9, trans-11 CLA content of muscle lipids by soybean oil but not by whole cotton seed.

Milk Production, Milk Composition, Live Weight Change and Milk Fatty Acid Composition in Lactating Dairy Cows in Response to Whole Linseed Supplementation

Suksombat, Wisitiporn,Meeprom, Chayapol,Mirattanaphrai, Rattakorn Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.8

The objective of this study was to determine the effects of whole linseed supplementation on performances and milk fatty acid composition of dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The control group received 300 g of palm oil. The second group was supplemented with 344 g/d of top-dressed whole linseed plus 150 g of palm oil and the third group was supplemented with 688 g/d of top-dressed whole linseed. All cows also received ad libitum grass silage (Brachiaria ruziziensis), had free access to clean water and were individually housed in a free-stall unit and individually fed according to treatments. Residual feeds were collected on 2 consecutive days weekly and at the end of the experiment. Feed samples were pooled to make representative samples for proximate and detergent analyses. Daily milk yields were recorded. Milk samples were collected on 2 consecutive days weekly. Live weights were recorded at the start and at the end of the experiment. Milk samples were taken on d 56 of the experiment and subjected to milk fatty acid composition. The results showed no statistical significant differences in intakes, live weight change, milk yields and milk compositions, however, C18:1, C18:3 and unsaturated FAs were increased while saturated FAs were reduced by whole linseed supplementation. It is recommended that the addition of 300 g/d oil from whole linseed could be beneficial to lactating dairy cows in early lactation.

Effects of Linseed Oil or Whole Linseed Supplementation on Performance and Milk Fatty Acid Composition of Lactating Dairy Cows

Suksombat, Wisitiporn,Thanh, Lam Phuoc,Meeprom, Chayapol,Mirattanaphrai, Rattakorn Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.7

The objective of this study was to determine the effects of linseed oil or whole linseed supplementation on performance and milk fatty acid composition of lactating dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The treatments consisted of basal ration (53:47; forage:concentrate ratio, on a dry matter [DM] basis, respectively) supplemented with 300 g/d of palm oil as a positive control diet (PO), or supplemented with 300 g/d of linseed oil (LSO), or supplemented with 688 g/d of top-dressed whole linseed (WLS). All cows were received ad libitum grass silage and individually fed according to the treatments. The experiment lasted for 10 weeks including the first 2 weeks as the adjustment period, followed by 8 weeks of measurement period. The results showed that LSO and WLS supplementation had no effects on total dry matter intake, milk yield, milk composition, and live weight change; however, the animals fed WLS had higher crude protein (CP) intake than those fed PO and LSO (p<0.05). To compare with the control diet, dairy cow's diets supplemented with LSO and WLS significantly increased milk concentrations of cis-9,trans-11-conjugated linoleic acid (CLA) (p<0.05) and n-3 fatty acids (FA) (p<0.01), particularly, cis-9,12,15-C18:3, C20:5n-3 and C22:6n-3. Supplementing LSO and WLS induced a reduction of medium chain FA, especially, C12:0-C16:0 FA (p<0.05) while increasing the concentration of milk unsaturated fatty acids (UFA) (p<0.05). Milk FA proportions of n-3 FA remarkably increased whereas the ratio of n-6 to n-3 decreased in the cows supplemented with WLS as compared with those fed the control diet and LSO (p<0.01). In conclusion, supplementing dairy cows' diet based on grass silage with WLS had no effect on milk yield and milk composition; however, trans-9-C18:1, cis-9,trans-11-CLA, n-3 FA and UFA were increased while saturated FA were decreased by WLS supplementation. Therefore, it is recommended that the addition 300 g/d of oil from whole linseed should be used to lactating dairy cows' diets.

Comparison of Different Alkali Treatment of Bagasse and Rice Straw

Suksombat, W. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.10

A study was conducted to determine the effect of different alkali treatments on changes in chemical composition and on degradability of bagasse and rice straw. This study divided into 2 experiments, the first with bagasse and the second with rice straw. Each experiment comprised 9 treatments which included: untreated control; 3% NaOH; 6% NaOH; 3% urea; 6% urea; 3% NaOH/3% urea; 3% NaOH/6% urea; 6% NaOH/3% urea; 6% NaOH/6% urea. In both experiments, crude protein contents were increased from 2.0 to 12.5 units for bagasse and 3.1 to 13.7 units for rice straw by urea treatments. Ash contents of the treated bagasse and rice straw were increased over the untreated control (1.5-9.7 units for bagasse; 4.2-8.8 units for rice straw). The effects on ether extract, crude fiber, neutral detergent fiber and acid detergent fiber of the treated bagasse and rice straw were variable. Nylon bag degradability of dry matter and crude fiber were increased by treatments applying NaOH and NaOH plus urea but not urea alone. In contrast, the egradability of neutral detergent fiber and acid detergent fiber were reduced compared with the untreated control. From these degradability studies, it can be concluded that the most efficient treatments of bagasse were those treatments with 6% NaOH, followed by treatments with 6% NaOH plus 3% or 6% urea and 3% NaOH plus 3% or 6% urea, respectively. However, when comparison was made on the cost of chemical used to treat the agricultural by-products, particularly in case of rice straw, 3-6% urea would be appropriate.

Effect of Cutting Interval and Cutting Height on Yield and Chemical Composition of Hedge Lucerne (Desmanthus virgatus)

Suksombat, Wisitiporn,Buakeeree, K. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.1

The experiment was conducted to determine the effects of cutting interval and cutting height on the yield and nutrient composition of hedge lucerne (Desmanthus virgatus) when grown on a sandy soil in the Northeast of Thailand. The cutting intervals compared were 30, 40 and 50 days between harvests and the cutting heights 30, 40 and 50 cm above ground level. The experiment was a $3{\times}3$ factorial layout in a randomized complete block design with 4 replications-giving a total of 36 plots each $3{\times}3m^2$. Harvested plant material was weighed, dried and the ground subsamples taken for analyses of crude protein (CP), crude fiber (CF), ash, ether extract (EE) and nitrogen-free extract (NFE). At the last harvest the hedge lucerne samples were separated to determine leaf to stem ratios and then analyzed for nutrient composition in the leaf and stem. Results showed that increasing the cutting interval (i.e. advancing age of maturity) increased dry matter and nutrient yields significantly. In terms of nutrient content, it also increased the crude fiber, ash, ether extract and nitrogen free extract percent in the plant. However, crude protein percent was markedly decreased as the cutting interval increased. Increasing cutting height had no effect on dry matter yield and yields of nutrients, but in terms of nutrient content, it increased crude protein and ash content, but decreased crude fiber content. The percent EE and NFE in the plant was unaffected by cutting height. From the results presented it is clear that cutting a stand of hedge lucerne every 40 to 50 days will achieve greater dry matter and nutrient yields than cutting more frequently, at 30 days. The cutting height at harvest, whether 30, 40 or 50 cm above ground level had no effect on dry matter or nutrient yields of hedge Lucerne. Hedge lucerne therefore offers the Thai poultry farmer a useful alternative protein supplement for poultry diets rather than relying on the more expensive soybean meal. As it can be readily and successfully grown on a range of soil types and climates throughout Thailand, hedge lucerne also offers the Thai farmer a valuable additional source of income.

Feeding of Whole Sugar Cane to Dairy Cattle during the Dry Season

Suksombat, W.,Mernkrathoke, P. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.3

A study was conducted to determine the effect of feeding chopped whole sugar cane compared to corn silage on performances of dairy cows during the dry season. Twenty four Holstein Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in mid lactation; averaging 16.5${\pm}$2.0 kg of milk, 121${\pm}$22 days in milk, 54.5${\pm}$4.5 months old and 440${\pm}$31 kg live weight, were stratified for milk yield, days in milk, age, stage of lactation and body weight, and then randomly allocated to two treatment groups (12 cows in each group). The first group was fed corn silage together with commercial concentrate while the second group was fed chopped whole sugar cane together with commercial concentrate. All cows consumed similar DM, however, cows on corn silage consumed more CP while cows on chopped whole sugar cane consumed more $NE_{LP}$. No significant differences in performances between the two groups were observed except for final live weight and body weight change. Cows on chopped whole sugar cane showed higher final live weight and gained more weight than cows on corn silage. The present study clearly indicates that chopped whole sugar cane can be fed to lactating dairy cows, while giving similar milk yield to corn silage.

Feeding of Sugar Cane Silage to Dairy Cattle during the Dry Season

Suksombat, Wisitiporn,Junpanichcharoen, P. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.8

A study was conducted to determine the effect of feeding sugar cane silage compared to chopped whole sugar cane or grass silage on performances of lactating dairy cows during the dry season. Twenty four Holstein Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in mid lactation; averaging 15.4${\pm}$3.2 kg of milk, 120${\pm}$23 days in milk, 50.5${\pm}$6.5 months old and 432${\pm}$39 kg live weight, were stratified for milk yield, days in milk, age, stage of lactation and body weight, and then randomly allocated to three treatment groups (8 cows in each group). All cows were fed 7.5 kg/d commercial concentrate plus ad libitum roughage according to treatment groups, which were grass silage, sugar cane silage or chopped whole sugar cane respectively. All cows consumed similar DM and produced similar milk and milk composition yields. However, cows on grass silage lost more weight than the other cows. The present study indicated that, during the dry season, sugar cane silage can be fed to lactating dairy cows, while giving similar milk yield to grass silage or chopped whole sugar cane.

Silage from Agricultural By-products in Thailand: Processing and Storage

Suksombat, W.,Lounglawan, P. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.4

Processing and storage of the silage from agricultural by-products were studied in two experiments. The first experiment was conducted to investigate the chemical composition and degradability of various silages with varying ensiling times. The experiment was a 5$\times$3 factorial design, completely randomized, with factor A as the different formulated mixtures by varying level of urea addition (0, 0.5, 1.0, 1.5 and 2.0%) and factor B as the time of ensiling. Chemical composition changed little with time and varied only slightly with levels of urea in the mixtures. Dry matter (DM) degradability increased with increasing cassava levels while crude protein degradability and pH level increased with increasing urea addition. By using 'Flieg point', which relates to organic acid yields, there were no significant difference among ensiled mixtures and times of ensiling. Therefore it can be concluded that the 5th silage formulation is the most appropriate since its DM and crude protein (CP) degradability were highest. The second experiment was carried out to determine the quality of the 5th silage mixtures (from the previous trial) after being stored for up to 6 months. The experiment was a complete randomized design with samples taken at monthly intervals up to 6 months and subjected to laboratory and degradability analyses. The results showed no significant (p>0.05) difference in chemical composition except for increased neutral detergent fiber and acid detergent fiber percentage in association with increasing storage time. There were no significant (p>0.05) differences in 'Flieg point' among times of storage. In conclusion, this experiment showed that the silage from agricultural by-products can be stored for more than 6 months.