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Evaluating the contribution of urban ecosystem services in regulating thermal comfort
Chaudhuri Suchismita,Kumar Amit 대한공간정보학회 2021 Spatial Information Research Vol.29 No.1
The study focuses on spatio-temporal dynamics of urban ecosystem services (UES) and their contribution in maintaining thermal comfort in Bhubaneswar city, India. An extensive increase in UES demand (grey) patches (187.95%) was observed during 1992–2016 in contrast to significant decline (47.94%) in UES supply (blue–green) patches, primarily in the northern and south–western directions. Also, a drastic rise in area under thermally highly uncomfortable zone (35–40 C) from 0.005 to 56.68 km2 and a decrease in area of thermally comfortable zone (B 26 C) from 0.46 km2 to zero during 1992–2016 exhibiting deteriorating natural urban living condition. Although, the land surface temperature (LST) was remained higher in urban areas, the peri-urban and neighbouring rural areas (27.31–33.98 C) of Bhubaneswar city recorded a high increase in mean LST as compared to the urban areas (31.19–34.69 C). In both the cases, UHI intensities were less as compared to other growing cities of India. The MODIS based time series analysis depicted similar trends with minor increase in LST (30.55–30.76 C) during 2000–16. The study proves the intrinsic linkages of UES with thermal comfort and necessitates to adopt sustainable measures to make the city green and habitable.
Sagarika Bohidar,Suchismita Pattanaik,Manikkannan Thiruvanoukkarasu 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
A rapid and efficient in vitro propagation protocolby enhanced multiple shoot proliferation from internodecultures of Ruta graveolens was established. Meanshoot number was maximum (55.83) in Murashige andSkoog (MS) basal medium fortified with 1.0 mg L-1benzyl amino purine and 0.25 mg L-1 indole-3-acetic acid. The elongated shoots rooted within 10–12 days in 1/2-strength MS medium supplemented with 2.0 mg L-1indole 3-butyric acid. About 80 % of the rooted plantletssurvived acclimatization and transfer to the field. Phytochemicalanalysis revealed that micropropagated plantsproduced linear furanocoumarins, characteristic of thespecies, in greater quantities as compared to the in vivogrownplants. The results will facilitate the conservation ofthis valuable medicinal plant and to obtain plants withimproved phytochemical constituents.
Bohidar, Sagarika,Pattanaik, Suchismita,Thiruvanoukkarasu, Manikkannan 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
A rapid and efficient in vitro propagation protocol by enhanced multiple shoot proliferation from internode cultures of Ruta graveolens was established. Mean shoot number was maximum (55.83) in Murashige and Skoog (MS) basal medium fortified with $1.0mgL^{-1}$ benzyl amino purine and $0.25mgL^{-1}$ indole-3-acetic acid. The elongated shoots rooted within 10-12 days in 1/2-strength MS medium supplemented with $2.0mgL^{-1}$ indole 3-butyric acid. About 80 % of the rooted plantlets survived acclimatization and transfer to the field. Phytochemical analysis revealed that micropropagated plants produced linear furanocoumarins, characteristic of the species, in greater quantities as compared to the in vivogrown plants. The results will facilitate the conservation of this valuable medicinal plant and to obtain plants with improved phytochemical constituents.
Yumnam, Silvia,Raha, Suchismita,Kim, Seong Min,Saralamma, Venu Venkatarame Gowda,Lee, Ho Jeong,Ha, Sang Eun,Heo, Jeong Doo,Lee, Sang Joon,Kim, Eun Hee,Lee, Won Sup,Kim, Jin A.,Kim, Gon Sup D.A. Spandidos 2018 ONCOLOGY REPORTS Vol.40 No.6
<P>Proteomic analysis serves as an important biological tool for identifying biological events. Novel biomarkers of a specific disease such as cancer may be identified using these promising techniques. The aim of the present study was to investigate the effect of tangeretin and to identify potential biomarkers in AGS gastric cancer cells using a proteomics approach. The results of the present study revealed that tangeretin inhibited AGS cell viability dose-dependently with a half-maximal inhibitory concentration of 100 µM. Two-dimensional gel electrophoresis was performed to determine the potential biomarker between control and tangeretin (100 µM)-treated AGS cells. A total of 16 proteins was identified from 36 significant protein spots using matrix-assisted laser-desorption/ionization time-of-flight-mass spectrometry using peptide fingerprinting. The bioinformatics tools Protein ANalysis THrough Evolutionary Relationships (PANTHER) and Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to identify the functional properties and association of the proteins obtained. Using western blot analysis, the regulatory pattern of four selected proteins, protein kinase Cε, mitogen-activated protein kinase 4, phosphoinositide 4-kinase and poly(ADP-ribose) polymerase 14, were successfully verified in replicate sample sets. These selected proteins are primarily involved in apoptosis signaling, angiogenesis, cell cycle regulation, receptor kinase binding, intracellular cytoplasmic and nuclear alterations. Therefore, aim of the present study was to identify potential diagnostic biomarkers from the functional categories of altered protein expression in tangeretin-inhibited AGS gastric cancer cell viability.</P>
Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array
Yumnam, Silvia,Saralamma, Venu Venkatarame Gowda,Raha, Suchismita,Lee, Ho Jeong,Lee, Won Sup,Kim, Eun Hee,Lee, Sang Joon,Heo, Jeong Doo,Kim, Gon Sup SPANDIDOS PUBLICATIONS 2017 MOLECULAR MEDICINE REPORTS Vol. No.
<P>Protein array technology not only identifies a large number of proteins but also determines their expression levels. In the present study, antibody array analysis is used to decipher the proteins involved in hesperidin-induced cell death in HepG2 cells. Altered proteins in hesperidin treated cells were compared with that of untreated control cells by using a RayBio<SUP>®</SUP> Label-based (L series) human antibody array kit. The identified proteins were further confirmed using western blot analysis. STRING software based analysis was used to determine the protein-protein interactions. Many proteins related to signal transduction, cellular mechanisms, cell growth and proliferation regulatory proteins were identified. Among the proteins identified Hsp90, Smac/DIABLO, Prdx6 and FRK were significantly reduced in hesperidin treated cells. To the best of the authors' knowledge, the present study is the first to use antibody array for identifying proteins marker in hesperidin-induced cell death in HepG2 cells. The present study provides a novel insight into the anticancer mechanism of hesperidin.</P>