연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

미들박스 링크 비용을 고려한 라우팅 비용 변화에 대한 연구

권수경(Su-Kyung Kwon),김태준(Tae-June Kim),김정구(Jeong-Goo Kim),최윤호(Yoon-Ho Choi) 한국통신학회 2015 한국통신학회 학술대회논문집 Vol.2015 No.06

유치원 교사의 변인에 따른 음악적 개념 인식 및 이해수준과 음악적 개념에 대한 자기인식과 실제이해의 차이

권수현(Kwon Su-hyun),박선미(Park Seon Mi) 한국육아지원학회 2013 육아지원연구 Vol.8 No.2

본 연구는 유치원 교사의 변인에 따른 음악적 개념에 대한 자기 인식과 실제 이해를 살펴보고 자기 인식과 실제 이해의 차이를 분석하고자 하였다. 연구대상은 서울시 소재의 유치원 교사 302명이며, Kim, Lee, Kwon과 Park(2011)의 유아교사 음악적 개념 실제 이해 측정 도구를 사용하여 자료를 수집 하였다. 첫째, 유치원 교사의 개인적 변인에 따라 음악적 개념에 대한 자기인식 정도에 차이가 있는가 를 살펴본 결과, 경력이 4년 이상 8년 미만이고, 교육정도가 대학원 이상이며, 음악관련 현직교육을 수 강한 경험이 있고, 양성과정에서 음악관련 수업을 들었고, 음악을 매우 즐기고, 악기교육을 4년 이상 받 은 경험이 있는 경우 자신이 음악적 개념에 대해 잘 알고 있다고 인식하는 정도가 높았다. 둘째, 유치 원 교사의 개인적 변인에 따라 음악적 개념에 대한 실제이해 정도에 차이가 있는가를 살펴본 결과, 경 력이 8년 이상이고, 교육정도가 대학원 이상이며, 근무기관이 국․공립 유치원이고, 음악관련 현직교육 을 수강한 경험이 있으며, 양성과정에서 음악관련 수업을 들었고, 악기교육을 4년 이상 받은 경험이 있 는 경우에 음악적 개념에 대한 실제이해가 높았다. 셋째, 유치원 교사의 음악적 개념에 대한 자기인식 과 실제이해에는 유의한 차이가 있는 것으로 나타났다. 이는 자신이 알고 있다고 생각하는 것과 실제로 아는 것은 다르다는 것을 의미한다. The purpose of this study was to examine the level of kindergarten teachers’ self-perception and actual understanding of the musical concepts according to six variables and to compare their self-perception with their actual understanding. The participants of this study were 302 kindergarten teachers in Seoul, and the data was collected using a measurement developed by Kim, Lee, Kwon & Park(2011). The results show, first, in terms of the teachers’ self-perception of the musical concepts related to the variables, significant differences were found in all the variables except for the types of kindergarten. In general, the teachers who had longer teaching career (4-8 years), higher educational level (graduate school or above), took music education related course(s) during pre-service education years, had taken music-related course(s) through in-service education programs, the more enjoyed music, had higher understanding about the musical concepts. Also, the teachers who had more learning experiences in musical instrument (over 4 years) had higher understanding about the musical concepts. Second, in terms of the teachers’ content knowledge of the musical concepts depending on the variables, statistically significant differences were shown in all of the variables except for the level of enjoying music. In other words, in general, the teachers who had longer teaching career (over 8 years), higher educational level (graduate school or above), worked in national public kindergarten, took music education course(s) during pre-service and in-service education programs, and had more learning experiences in musical instrument (over 4 years) showed higher understanding level about the musical concepts. Third, the level of kindergarten teachers’ perceived knowledge in the musical concepts was significantly higher than the level of their actual knowledge.; there was a considerable gap between perceived knowledge and actual knowledge of musical concepts.

치매(痴매)의 침구치료(鍼灸治療)에 대(對)한 문헌적(文獻的) 고찰(考察)

김동석 ( Dong Suk Kim ),권순철 ( Sun Chul Kwon ),서상수 ( Sang Su Seo ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.21 No.2

Objectives: This study is designed to investigate the pathology, acupuncture and moxibustion therapy applicable of the dementia through literature of oriental medicine. Results: The findings of this study are as follows; 1. The cause of dementia is internal damage of seven emotions(七情內傷), deficiency of the kidney essence(腎精不足), retention of dampness in the mental(痰迷心竅). 2. The acupuncture meridians used often for dementia are Tok-maek(GV), Chok-t`aeyang Pang-gwang-Kyong(BL), Chok-yangmyong Wi-Kyong(ST), Su-gworum Shimp`o-Kyong(PC), Su-soum Shim-Kyong(HT). 3. The spots on the body suiTable for acupuncture used for therapy of the dementia are 98 points and extra points are 6 points, total therapeutic acupunture points are 104 points. And acupunture points are frequently used Shinmun(神門-HT7, 24), Paek`oe(百會-GV20, 19), Chok-samni(足三里-ST36, 18), Sugu(水溝-GV26, 17), P`ungnyung(豊隆-ST40, 16), Taech`u(大椎-GV14, 14), Samumgyo(三陰交-SP6, 14), Shimsu(心兪-BL15, 14), Taenung(大陵-PC7, 11), Nogung(勞宮-PC8, 11), Sosang(少商-LU11, 10), Kansa(間使-PC5, 10), Naegwan(內關-PC6, 10). 4. The acupoints used often on moxibustion are Paek`oe(百會-GV20), Chang-gang(長强-GV1), Taech`u(大椎-GV14), Kansa(間使-PC5). Conclusion: It is recommended that further study of many sided investigations in the future.

치매(痴?)의 침구치료(鍼灸治療)에 대(對)한 문헌적(文獻的) 고찰(考察)

김동석,서상수,권순철,Kim, Dong-Suk,Seo, Sang-Su,Kwon, Sun-Chul 경락경혈학회 2004 대한침구의학회지 Vol.21 No.2

Objectives : This study is designed to investigate the pathology, acupuncture and moxibustion therapy applicable of the dementia through literature of oriental medicine. Results : The findings of this study are as follows ; 1. The cause of dementia is internal damage of seven emotions(七情內傷), deficiency of the kidney essence(腎精不足), retention of dampness in the mental(痰迷心竅). 2. The acupuncture meridians used often for dementia are $Tok-maek(GV),\;Chok-t'aeyang\;Pang-gwang-Ky\check{o}ng(BL),\;Chok-yangm\check{o}yng\;Wi-Ky\check{o}ng(ST),\;Su-gwor\check{u}m\;Shimp'o-Ky\check{o}ng(PC),\;Su-so\check{u}m\;Shim-Ky\check{o}ng(HT)$. 3. The spots on the body suiTable for acupuncture used for therapy of the dementia are 98 points and extra points are 6 points, total therapeutic acupunture points are 104 points. And acupunture points are frequently used Shinmun(神門-HT7, 24), Paek'oe(百會-GV20, 19), Chok-samni(足三里-ST36, 18), Sugu(水溝-GV26, 17), P'ungnyung(豊隆-ST40, 16), Taech'u(大椎-GV14, 14), $Sam\check{u}mgyo(三陰交-SP6,\;14)$, Shimsu(心兪-BL15, 14), $Taen\check{u}ng(大陵-PC7,\;11)$, Nogung(勞宮-PC8, 11), Sosang(少商-LU11, 10), Kansa(間使-PC5, 10), Naegwan(內關-PC6, 10). 4. The acupoints used often on moxibustion are Paek'oe(百會-GV20), Chang-gang(長强-GV1), Taech'u(大椎-GV14), Kansa(間使-PC5). Conclusion : It is recommended that further study of many sided investigations in the future.

일 전문대학 간호학생의 건강상태 조사연구

권수자 서라벌대학 1995 논문집 Vol.9 No.-

The Purpose of this study was to investigate the health status of nursing students. Subjects of this study were 230 nursing students of Kyong Ju Junior College in 1994. The data were analyzed by prcentage, F-test and Pearson correlation. The results were as follows: 1. Total THI mean score was not significant according to the grade, but nursign students were somewhat normal health status(freshman was 253.2, sophomore was 252.4, junior was 249.4). Total personality & mind item was significnt(p<0.05). 3. According to the satisfaction of the college life, the THI mean score was significant in the personality & mind item(p<0.01). 4. The grade & health status were not correlated. 5. According to the correlation coefficient between satisfaction of the college life & health status : Total THI score was correlated (r=.22), Personality & mind were correlated (r=.27), Irregular life stress was correlated(r=.30).

무균돼지 사육시설 계획을 위한 사례연구 : 공간구성 및 동선계획을 중심으로

권순정,성제경,염수청 한국의료복지시설학회 2006 의료·복지 건축 Vol.12 No.1

According to the increase of demand for human organs such as kidney, heart, pancreas, joint, and cornea for therapeutic transplantation, the production of alternative organs based on Gnotobiotic Pigs gains a lot of concerns all over the world. However, it is not common to design and build Gnotobiotic Pigs' facility, and there are only a few those facilities and planning principles for them. Considering the situation above, this paper tries to develop planning guidelines for space organization and circulation system of standardized Germ Free Pig's facility on the bases of case analysis. The results of this study are as follows. At first, four swine farms including a Gntobiotic Pig's facility has been analysed from the point of space organization and circulation system. Secondly, the space zoning of Gnotobiotic Pigs' facility has been proposed into 5 groups : pigs' area, adminstration area, operating room and laboratory, service area, and mechanical area. Space components of each group have been presented also. Finally, circulation system of Gnotobiotic Pigs' facility has been explored from a operational point of view. This, also, includes human circulation, pig's circulation, and goods' circulation. This study has some limitations because it does not consider the SOPs(standard operational policies) of that facility to the fullest measure and does not suggest space area of each part, either. Despite of some weaknesses, it is expected that this study can give some useful guidelines for the design and planning of Germ Free Pigs' facilities.

국내 혈액질환 환자에서 침습성 아스페르길루스증의 구제치료로서 Voriconazole의 효과와 안전성

권재철,김시현,최수미,최재기,이동건,박선희,최정현,유진홍,신완식 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Invasive aspergillosis (IA) is associated with significant morbidity and mortality in patients with hematologic malignancies. We investigated the efficacy and safety of voriconazole (VCZ) when used as salvage therapy for IA in Korean adults with hematologic malignancies who had not responded to prior antifungal therapy. Materials and Methods: We retrospectively reviewed data, collected from January 2007 to October 2008, from patients with proven or probable cases of IA. All were probable IA cases, except for one proven case. All cases were refractory or intolerant to antifungal therapy prior to administration of VCZ. Efficacy and safety were assessed in patients treated with VCZ for more than 3 days and for more than one dose, respectively. A favorable response [complete (CR) or partial (PR)] was defined by significant improvement of all clinical symptoms, signs, and radiologic abnormalities. Results: Fifty patients who met the inclusion criteria were enrolled. There were 27 male and 23 female patients with mean age of 44.4 years (range, 15-65 years). Underlying diseases were acute leukemia (35 cases), chronic myelogenous leukemia (4 cases), myelodysplastic syndrome (3 cases), lymphoma (3 cases) and other hematologic diseases (5 cases). Twenty-two patients had received chemotherapy and 13 patients had undergone hematopoietic stem cell transplantation. The lung was the main infection site (94%) followed by the sinus (6%). Amphotericin B deoxycholate alone was the most frequent previous antifungal therapy. The mean duration of antifungal therapy prior to VCZ therapy was 13.9±8.8 days (2-44 days). The median duration of VCZ therapy was 19 days (interquartile range, 49 days). Sixteen patients (32.0%) showed favorable responses (CR:PR=8:8) at the end of VCZ therapy. The numbers of patients with stable disease, progression and death were, 6 (12%), 6 (12%) and 22 (44%) respectively. Most of those with unfavorable responses had relapsed underlying malignancies or refractory graft versus host diseases. Twelve patients developed drug-related adverse events but only one patient stopped VCZ treatment prematurely. Conclusions: VCZ demonstrated an acceptable level of toxicity in patients with hematologic malignancies but further studies are required to prove its efficacy as salvage therapy.

외래환자가 지각한 의료 서비스에 대한 만족도 평가

권수자 서라벌대학 1994 논문집 Vol.8 No.-

The purpose of this study was to investigate patient satisfaction about the medical care services in outpatient department. The subjects of this study were 472 outpatients. The data were collected from 29th April to 7th May, 1993, using questionnaire and analized by spss program (percentage, mean, T-test, Anova). The Mean score of satisfaction were as follows: 1. Spending time of the medical care service ; Mean score was 2.770. 2. Guidance of the facilities ; Mean score was 3.535. 3. Cost of medical care service ; Mean score was 2.818. 4. Attitude of the staff with sincerity ; Mean score was 3.368. 5. Explanation of the curative service ; Mean score was 3.327. 6. OPD environment ; Mean score was 2.932.

NaOCl의 적용 후 치수강 상아질에 대한 결합강도의 변화와 Sodium Ascorbate에 의한 환원 효과

권수미,김태균,유미경,이광원 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6

본 연구에서는 치수강이 개방된 근관와동에 5.25% NaOCl용액을 각각 다른 시간 동안 적용시킨 뒤 접착레진에 의한 미세인장결합강도의 변화를 측정하였으며, 10% sodium ascorbate를 NaOCl 용액에 일정시간 노출시킨 상아질에 다시 10분 동안 적용하여 결합강도에 미치는 효과를 연구하고자 하였다. 연구결과 본 실험에서 상부 치수강 상아질과 하부 치수강 상아질에 대한 결합강도는 모든 실험군내에서 유의한 차이 (p > 0.05)를 보이지 않았다. 5.25% NaOCl 용액을 적용하지 않고 치수강 상아질에 접착시킨 대조군에 비해 20분간 적용시킨 실험군에서는 결합강도의 감소가 초래되었으나 통계적인 유의성 (p > 0.05)은 없었다. 그러나 5.25% NaOCl 용액을 40분과 80분 동안 치수강 상아질에 적용시킨 실험군에서는 대조군에 비해 유의하게 낮은(p < 0.05) 결합강도의 변화를 나타내었다. 5.25% NaOCl 용액을 40분동안 그리고 10% sodium ascorbate를 10분간 처리한 실험군에서는 유의한 결합강도의 회복을 나타내었다. 그러나 5.25% NaOCl 용액을 80분 동안 그리고 10% sodium ascorbate를 10분간 적용한 실험군에서는 대조군에 비해 통계적으로 유의한 차이(p < 0.05)를 보임으로써 여전히 결합강도가 회복되지 못하고 있음을 나타내었다. 5.25% NaOCl 용액을 5분간 적용한 뒤 10% sodium ascorbate를 10분간 적용한 실험군에서는 오히려 대조군에 비해 높은 결합강도(p < 0.05)를 보여줌으로써 높은 회복효과를 나타내었다. Clinical suggestion for the limitation of application time of NaOCl solution is needed to avoid large reductions in resin-dentin bond strength. The aim of this study was to measure the change of -tensile bond strength after the various application time of 5.25% NaOCl solution to pulp chamber dentin in endodontic access cavity, and to evaluate the effect of 10% sodium ascorbate application for 10 min on bond strength after the treatment of 5.25% NaOCl solution. In this experiment, there were no statistical differences(p > 0.05) in bond strengths between upper chamber dentin and lower chamber dentin. NaOCl-treated group for 20 min did not show any significant decrease(p > 0.05) in bond strength than non-treated control group. In contrast to that, bond strengths of NaOCl-treated groups for 40 & 80 min were significantly lower(p < 0.05) than that of non-treated control group. 10% sodium ascorbate retreated group for 10 min after 5.25% NaOCl application for 40 min to chamber dentin showed the recovery of bond strength significantly. However, the bond strength of sodium ascorbate retreated group after 5.25% NaOCl application for 80 min was still significantly lower(p < 0.05) compared to the non-treated control group, which means the reductions in resin-dentin bond strength were not fully reversed. On the contrary, sodium ascorbate retreated group after 5.25% NaOCl application for 5 min showed significantly higher(p < 0.05) bond strength compared to the control group, which demonstrates its superior recovery effect. In SEM exminations of specimens retreated with 10% sodium ascorbate after NaOCl application for 40 & 80 min showed that resin tags were formed clearly and densely, but weakly in density and homogeneity of individual resin tag compared to the control specimen.