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Linghao Zhang,Lu Zhang,Chun-Hong Zhang,Xiao-Bi Fang,Zhen-Xiao Huang,Qing -Yuan Shi,Li-Ping Wu,Peng Wu,Zhen-Zhen Wang,Zhi-Su Liao 대한이비인후과학회 2016 Clinical and Experimental Otorhinolaryngology Vol.9 No.4
Objectives. Chronic rhinosinusitis (CRS) is common disease in otorhinolaryngology and will lead to lower airway abnormality. However, the only lung function in CRS patients and associated factors have not been much studied. Methods. One hundred patients with CRS with nasal polyps (CRSwNP group), 40 patients with CRS without nasal polyps (CRSsNP group), and 100 patients without CRS were enrolled. The difference in lung function was compared. Meanwhile, CRSwNP and CRSsNP group were required to undergo a bronchial provocation or dilation test. Additionally, subjective and objective outcomes were measured by the visual analogue scale (VAS), 20-item Sino-Nasal Outcome Test (SNOT-20), Lund-Mackay score, Lund-Kennedy endoscopic score. The correlation and regression methods were used to analyze the relationship between their lung function and the above parameters. Results. The forced expiratory volume in 1 second (FEV1) and forced expiratory flow between 25% and 75% of forced vital capacity (FEF25-75) of CRSwNP group were significantly lower than other groups (P<0.05). On peak expiratory flow, there was no difference between three groups. In CRSwNP group, FEV1 was negatively correlated with peripheral blood eosinophil count (PBEC) and duration of disease (r=–0.348, P=0.013 and r=–0.344, P=0.014, respectively), FEF25-75 negatively with VAS, SNOT-20 (r=–0.490, P=0.028 and r=–0.478, P=0.033, respectively) in CRSsNP group. The incidence of positive bronchial provocation and dilation test was lower in CRSwNP group (10% and 0%, respectively), with both 0% in CRSsNP group. The multiple linear regression analysis indicated that change ratio of FEV1 before and after bronchial provocation or dilation test were correlated with PBEC in CRSwNP group (β=0.403, P=0.006). Conclusion. CRS leading to impaired maximum ventilation and small airway is associated with the existence of nasal polyp. Lung function impairments can be reflected by PBEC, duration, VAS, and SNOT-20. In CRSwNP patients, PBEC is independent predictor of FEV1 change ratio.
Su, Shu,Zhou, Hao,Xue, Meng,Liu, Jing-Yu,Ding, Lei,Cao, Meng,Zhou, Zhen-Xian,Hu, Hong-Min,Wang, Li-Xin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
The majority of hepatocellular carcinoma (HCC) patients have a poor prognosis with current therapies, and new approaches are urgently needed. We have developed a novel therapeutic cancer vaccine platform based on tumor cell derived autophagosomes (DRibbles) for cancer immunotherapy. We here evaluated the effectiveness of DRibbles-pulsed dendritic cell (DC) immunization to induce anti-tumor immunity in BALB/c mouse HCC and humanized HCC mouse models generated by transplantation of human HCC cells (HepG2) into BALB/c-nu mice. DRibbles were enriched from H22 or BNL cells, BALB/c-derived HCC cell lines, by inducing autophagy and blocking protein degradation. DRibbles-pulsed DC immunization induced a specific T cell response against HCC and resulted in significant inhibition of tumor growth compared to mice treated with DCs alone. Antitumor efficacy of the DCs-DRibbles vaccine was also demonstrated in a humanized HCC mouse model. The results indicated that HCC/DRibbles-pulsed DCs immunotherapy might be useful for suppressing the growth of residual tumors after primary therapy of human HCC.
Adaptation of murine anaplastic thyroid cancer cell line from hybrid to inbred C57BL/6 mice
Zhen Xu,Seong Yun Ha,Hyo Shik Shin,Minjun Kim,Su-jin Kim,Jae-Kyung Won,Seong-Hyeon Kim,Sun Wook Cho,Kyu Eun Lee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Background: Immuno-competent mouse model is indispensable for the study of tumor immune microenvironment. TBP-3743 cell line is derived from anaplastic thyroid cancer arising in immune-competent B6129SF1 mouse with thyroid-specific BrafV600E/WT and deletion of Trp53. Aim of this study is to adapt TBP-3743 cell line to C57BL/6 stain, widely used mouse strain which has same MHC haplotype with that of B6129SF1. Methods: TBP-3743 cell line was subcutaneously injected into C57BL/6 mice. The mouse with the rapidest tumor growth was sacrificed for primary culture and the cultured cells were injected into naive C57BL/6 mice. This serial of processes, called adaptation, has been performed three times. The concentration-dependent growth analysis is performed by comparing the tumor growth of 1x107-injected and 5x106-injected group. Furthermore, Original TBP-3743 cells and adapted TBP-3743 cells were treated with BRAFV600E inhibitor(PLX-4032, 1uM) in vitro and cell proliferation was measured by luminometric detection. For Immunotherapy, mice were treated 10mg/kg of PLX-4032 on daily basis, 15mg/kg of anti-CD47 every other day, 10mg/kg of anti-PD-L1 every other day Results: In the course of adaptation, the lag phage of tumor growth shortened and tumor growth rate had dramatically increased. In vitro growth of Both TBP-3743 and adapted TBP-3743(TBP-3743B6) were effectively BRAFV600E-selective inhibitor (PLX-4032). In vivo treatment of PLX-4032 with anti-CD47 and/or anti-PD-L1 reduced tumor volume in mice injected with TBP-3743B6 and prolonged their survival compared to control Conclusions: We established a novel immunocompetent murine anaplastic thyroid cancer model available in C57BL/6 inbred mice. TBP-3743B6 grows rapidly and stably in immunocompetent C57BL/6 mice. This mouse model can save unnecessary time, cost and labor for the research of tumor immune microenvironment. Also, this newly established cell line will be a useful resource to expand the feasibility of basic research as well as drug screening for potential therapeutic effects on anaplastic thyroid cancer.
A morphological study on autumnal morphs of Aphis glycines (Hemiptera: Aphididae)
Zhen-qi Tian,Su-ji Wang,Bing Bai,Jian Liu,Kuijun Zhao 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.2
The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the most important pests of soybean. The complex life cycle of A. glycines is characterized as heteroecious and holocyclic, and has seasonal polymorphisms occurring during its life cycle. In the autumn, A. glycines occur as winged and wingless virginoparae in soybeans, as gynoparae and males that migrate from soybeans to Rhamnus spp. and as gynoparae, males and oviparae on Rhamnus spp. In this study, wingless virginoparae, gynoparae, males and oviparae of A. glycines were successfully induced in the laboratory and morphological parameters of these morphs were selected for quantitation. To aid in identification of these A. glycines autumnal morphs, these aphids were imaged by microscope and significant differences in morphological characteristics were found: distal parts of hind leg femurs of gynoparae were grayish black, which were darker than those of winged virginoparae; the 4th instars of gynoparae, males and winged virginoparae differ greatly in dorsal abdomen coloration and covering. Our results provide an important guide for distinguishing adults of gynoparae and winged virginoparae, and for identifying nymphs of gynoparae, males and winged virginoparae.
Time-dependent Optimal Heater Control in Thermoforming Preheating Using Dual Optimization Steps
Zhen-Zhe Li,Kwang-Su Heo,Seoug-Yun Seol 한국정밀공학회 2008 International Journal of Precision Engineering and Vol.9 No.4
Thermoforming is one of the most versatile and economical processes available for shaping polymer products, but obtaining a uniform thickness of the final product using this method is difficult. Heater power adjustment is very important because the thickness distribution depends strongly on the distribution of the sheet temperature. In this paper; the steady-state optimum distribution of heater power is first ascertained by a numerical optimization to obtain a uniform sheet temperature. The time-dependent optimal heater input is then determined to decrease the temperature difference through the direction of the thickness using the response surface method and the D-optimal method. The optimal results show that the time-dependent optimum heater power distribution gives an acceptable uniform sheet temperature in the forming temperature range by the end of the heating process.
Su, Xinhui,Cheng, Kai,Jeon, Jongho,Shen, Bin,Venturin, Gianina Teribele,Hu, Xiang,Rao, Jianghong,Chin, Frederick T.,Wu, Hua,Cheng, Zhen American Chemical Society 2014 MOLECULAR PHARMACEUTICS Vol.11 No.11
<P>The epidermal growth factor receptor (EGFR) serves as an attractive target for cancer molecular imaging and therapy. Our previous positron emission tomography (PET) studies showed that the EGFR-targeting affibody molecules <SUP>64</SUP>Cu-DOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-FBEM-Z<SUB>EGFR:1907</SUB> can discriminate between high and low EGFR-expression tumors and have the potential for patient selection for EGFR-targeted therapy. Compared with <SUP>64</SUP>Cu, <SUP>18</SUP>F may improve imaging of EGFR-expression and is more suitable for clinical application, but the labeling reaction of <SUP>18</SUP>F-FBEM-Z<SUB>EGFR:1907</SUB> requires a long synthesis time. The aim of the present study is to develop a new generation of <SUP>18</SUP>F labeled affibody probes (Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB>) and to determine whether they are suitable agents for imaging of EGFR expression. The first approach consisted of conjugating Z<SUB>EGFR:1907</SUB> with NOTA and radiolabeling with Al<SUP>18</SUP>F to produce Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB>. In a second approach the prosthetic group <SUP>18</SUP>F-labeled-2-cyanobenzothiazole (<SUP>18</SUP>F-CBT) was conjugated to Cys-Z<SUB>EGFR:1907</SUB> to produce <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB>. Binding affinity and specificity of Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> to EGFR were evaluated using A431 cells. Biodistribution and PET studies were conducted on mice bearing A431 xenografts after injection of Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> or <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> with or without coinjection of unlabeled affibody proteins. The radiosyntheses of Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> were completed successfully within 40 and 120 min with a decay-corrected yield of 15% and 41% using a 2-step, 1-pot reaction and 2-step, 2-pot reaction, respectively. Both probes bound to EGFR with low nanomolar affinity in A431 cells. Although <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> showed instability <I>in vivo</I>, biodistribution studies revealed rapid and high tumor accumulation and quick clearance from normal tissues except the bones. In contrast, Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> demonstrated high <I>in vitro</I> and <I>in vivo</I> stability, high tumor uptake, and relative low uptake in most of the normal organs except the liver and kidneys at 3 h after injection. The specificity of both probes for A431 tumors was confirmed by their lower uptake on coinjection of unlabeled affibody. PET studies showed that Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> could clearly identify EGFR positive tumors with good contrast. Two strategies for <SUP>18</SUP>F-labeling of affibody molecules were successfully developed as two model platforms using NOTA or CBT coupling to affibody molecules that contain an N-terminal cysteine. Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> and <SUP>18</SUP>F-CBT-Z<SUB>EGFR:1907</SUB> can be reliably obtained in a relatively short time. Biodistribution and PET studies demonstrated that Al<SUP>18</SUP>F-NOTA-Z<SUB>EGFR:1907</SUB> is a promising PET probe for imaging EGFR expression in living mice.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mpohbp/2014/mpohbp.2014.11.issue-11/mp5003043/production/images/medium/mp-2014-003043_0008.gif'></P>
( Su Hyun Cho ),( Min Ju Choi ),( Zhen Long Zheng ),( Do Young Kim ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: Intralesional corticosteroid injection is a commonly used first line therapy for alopecia areata (AA). However, assessing the treatment response clinically can be troublesome and can vary between individuals. Objectives: To trace the changes in dermoscopic findings of active AA lesions after repeated intralesional corticosteroid injection treatment. Methods: Fifty six AA patients who initially showed a positive hair pull test were enrolled. Intralesional triamcinolone acetonide injection at a concentration of 5 mg/ml was done at a 3-6 weeks interval. Serial dermoscopic examinations at the preselected alopecic patches were performed initially and before every injection, which was continued until the disease activity turned negative. Results: Among the 56 AA patients, the mean age was 32.8±12.5 years, and the mean duration of AA lesions were 11.2±17.4 weeks. Initially, yellow dots were the predominant dermoscopic finding seen in 51 patients (91.1%), followed by black dots (64.3%). When the AA lesions clinically stabilized, short vellus hair was the predominant finding seen in 54 patients (96.4%), and black dots were still observed in 50% of patients. Conclusion: Although dermoscopic examination is frequently used in AA, studies about its use as an assessment tool of treatment response is rare. It can be used as an ancillary tool to estimate disease activity of AA, and also can be helpful in deciding when the intralesional corticosteroid injection should be discontinued.
Mocklinter : Linting Mutual Exclusive Deadlocks with Lock Allocation Graphs
Zhen Yu,Xiaohong Su,Tiantian Wang,Peijun Ma 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.3
Deadlocks are serious runtime bugs and are difficult to expose, reproduce and diagnose. Once suffering from them, programs may be afflicted with increasing response time, decreasing throughputs, or even crashes. We present Mocklinter, a dynamic deadlock detection tool to capture a deadlock as soon as it happens and spit out enough information to support source-level debugging. Mocklinter tracks the synchronization state of the target program by dynamically constructing and maintaining a lock allocation graph. Mocklinter uses this graph to decide whether a deadlock is confronted or not. Mocklinter handles all types of pthread mutexes and can detect any number of deadlocks at a time. Each deadlock captured by Mocklinter can involve any number of threads. We implemented Mocklinter in Linux-3.2.0 and evaluated it with ten applications, including Dining-Philosophers, Sshfs, SQLite, OpenLDAP, MySQL and so on, whose sizes varies from 0.1K to 1021.0K in terms of LOC. The results demonstrate effectiveness against real or artificial deadlock bugs, while incurring modest performance overhead and scaling to more than one thousand of threads.