Production and export of the parasitoid Tachardiaephagus somervilli (Chalcidoidea: Encyrtidae), a biological control agent for the yellow lac scale, Tachardina aurantiaca (Hemiptera: Coccoidea: Kerriidae)

Su Ping Ong,Dennis J. O’Dowd,Peter T. Green 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

Development and reproduction of the hymenopteran parasitoid Tachardiaephagus somervilli Mahdihassan (Chalcidoidea: Encyrtidae) were studied in its native range in Malaysia as part of a classical biological control program on Christmas Island (Indian Ocean). Tachardiaephagus somervilli was reared in the laboratory on its target host, the yellow lac scale, Tachardina aurantiaca Cockerell (Coccoidea: Kerriidae). The developmental time, survival, and sex ratio of F1 offspring from two different sources of F0 adults (field-collected F0 adults [=‘wild-caught’] and F0 adults that emerged from parasitized hosts in the laboratory [=‘laboratory-emerged’]) were compared. Sources of F0 adults did not affect the developmental time of either male or female offspring, which ranged from 23 to 25 days. Furthermore, per capita fecundity of laboratory-emerged F0 females was three times greater than wild-caught F0 females. The number of emerged F1 adults was positively related to host size. Tachardiaephagus somervilli has a short generation time relative to its female Tachardina hosts (~23 vs ~90 days), high fecundity (~16 emerged offspring per laboratory-emerged female) and can potentially complete 12–15 generations per year. This rearing protocol produced sufficient quantities of adult T. somervilli for the export and successful introduction to Christmas Island (Indian Ocean) for the biological control of invasive T. aurantiaca, with near 100% survival rates for both sexes during transit.

Dynamic analysis of Ca^(2+) level during bovine oocytes maturation and early embryonic development

Su Li Liang,Qian Jun Zhao,Xiang Chen Li,Ya Ping Jin,Yi Peng Wang,Xiao Hua Su,Wei Jun Guan,Yue Hui Ma 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.2

Mammalian oocyte maturation and early embryo development processes are Ca^(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca^(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca^(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca^(2+)was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca^(2+) was present throughout the blastomere. In PA embryos, Ca^(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca^(2+) in the SCNT embryos. However, Ca^(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca^(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca^(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate.

Cytogenetic and Genetic Mutation Features of de novo Acute Myeloid Leukemia in Elderly Chinese Patients

Su, Long,Li, Xian,Gao, Su-Jun,Yu, Ping,Liu, Xiao-Liang,Tan, Ye-Hui,Liu, Ying-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objectives: The present study aimed to examine the cytogenetic and genetic mutation features of acute myeloid leukemia (AML) in elderly Chinese patients. Methods: A retrospective analysis of cytogenetics and genetic mutations was performed in 113 cases (age range 50-82 years) with de novo AML. Results: The most frequent cytogenetic abnormality was t (15;17) (q22;q21), detected in 10.0% (n = 9) of successfully analyzed cases, followed by t (8;21) (q22;q22) in 8.89% (n = 8), and complex karyotypes in 5.56% (n = 5). Those with complex karyotypes included 4 cases (4.44%) of monosomal karyotypes. The frequencies of NPM1, FLT3-ITD, c-kit, and CEBPA mutations were 27.4% (31/113), 14.5% (16/110), 5.88% (6/102), and 23.3% (7/30), respectively. The complete remission rates of patients in low, intermediate, and high risk groups were 37.5%, 48.6%, and 33.3%, respectively (${\chi}^2$ = 0.704, P = 0.703) based on risk stratification. Conclusion: Cytogenetics and genetic mutations alone may not be sufficient to evaluate the prognoses of elderly AML patients. The search for a novel model that would enable a more comprehensive evaluation of this population is therefore imperative.

CHARACTERIZING THE TIME-FREQUENCY PROPERTIES OF THE 4 Hz QUASI-PERIODIC OSCILLATION AROUND THE BLACK HOLE X-ray BINARY XTE J1550-564

SU, YI-HAO,CHOU, YI,HU, CHIN-PING,YANG, TING-CHANG,HSIEH, HUNG-EN,CHUANG, PO-SHENG,LIN, CHING-PING,LIAO, NAI-HUI The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

We present the results from analysis of the Hilbert-Huang transform (HHT) for the 4 Hz quasi-periodic oscillations (QPO) around the black hole X-ray binary XTE J1550-564. The resultant Hilbert spectra demonstrate that the QPO is composed of a series of intermittent signals appearing occasionally. From the analysis of the HHT, we further found the distribution of the lifetimes for the intermittent oscillations and the distribution for the time intervals with no significant signal (the break time). The mean lifetime is 1.45 s and 90% of the oscillation segments have lifetimes less than 3.1 s whereas the mean break time is 0.42 s and 90% of break times are less than 0.73 s. We conclude that the intermittent feature of the QPO could be explained by the Lense-Thirring precession model and rules out interpretations of continual frequency modulation.

Chromophorylation of a Novel Cyanobacteriochrome GAF Domain from Spirulina and Its Response to Copper Ions

( Su-dan Jiang ),( Yi Sheng ),( Xian-jun Wu ),( Yong-li Zhu ),( Ping-ping Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.2

Cyanobacteriochromes (CBCRs) are phytochrome-related photoreceptor proteins in cyanobacteria and cover a wide spectral range from ultraviolet to far-red. A single GAF domain that they contain can bind bilin(s) autocatalytically via heterologous recombination and then fluoresce, with potential applications as biomarkers and biosensors. Here, we report that a novel red/green CBCR GAF domain, SPI1085g2 from Spirulina subsalsa, covalently binds both phycocyanobilin (PCB) and phycoerythrobilin (PEB). The PCB-binding GAF domain exhibited canonical red/green photoconversion with weak fluorescence emission. However, the PEB-binding GAF domain, SPI1085g2-PEB, exhibited an intense orange fluorescence (λ_{abs. max} = 520 nm, λ_{fluor. max} = 555 nm), with a fluorescence quantum yield close to 1.0. The fluorescence of SPI1085g2-PEB was selectively and instantaneously quenched by copper ions in a concentration-dependent manner and exhibited reversibility upon treatment with the metal chelator EDTA. This study identified a novel PEB-binding cyanobacteriochrome-based fluorescent protein with the highest quantum yield reported to date and suggests its potential as a biosensor for the rapid detection of copper ions.

恨世張愛玲 -還本色張愛玲之二

왕소평 ( Su Ping Wang ),엄영욱,염숙 韓國中國學會 2009 中國學報 Vol.59 No.-

An Improved Ant Colony Optimization for VRP with Time Windows

Yu Su-Ping,Mao Wei-Wei 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

The Vehicle Routing Problem (VRP) is an important problem occurring in many distribution systems, which is also defined as a family of different versions such as the Capacitated Vehicle Routing Problem (CVRP) and the Vehicle Routing Problem with Time Windows (VRPTW). The Ant Colony Optimization (ACO) is a metaheuristic for combinatorial optimization problems. Given the ACO inadequacy, the vehicle routing optimization model is improved and the transfer of the algorithm in corresponding rules and the trajectory updated regulations is reset in this paper, which is called the Improved Ant Colony Optimization (I-ACO). Compared to the calculated results with genetic algorithm (GA) and particle swarm optimization (PSO), the correctness of the model and algorithm is verified. Experimental results show that the I-ACO can quickly and effectively obtain the optimal solution of VRFTW.

Pathologic Response During Chemo-radiotherapy and Variation of Serum VEGF Levels Could Predict Effects of Chemo-Radiotherapy in Patients with Esophageal Cancer

Yu, Jing-Ping,Lu, Wen-Bin,Wang, Jian-Lin,Ni, Xin-Chu,Wang, Jian,Sun, Zhi-Qiang,Sun, Su-Ping Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Background: To investigate the relationship between pathologic tumor response to concurrent chemoradiotherapy and variation of serum VEGF in patients with esophageal cancer. Materials and Methods: Forty six patients with esophageal cancer who were treated with concurrent chemo-radiotherapy were enrolled. Endoscopic and pathologic examination was conducted before and four weeks afterwards. Serum level of VEGF was documented before, four weeks later and after chemo-radiotherapy. The relationship between pathologic response and the variation of serum level of VEGF and its influence on the prognosis were investigated. Results: Serum level of VEGF decreased remarkably during and after chemo-radiotherapy in patients whose pathologic response was severe (F=5.393, 4.587, P(0.05). There were no statistical differences of serum VEGF level before, during and after chemo-radiotherapy for patients whose pathologic response was moderate or mild. There were 18 (85.7%), 7 (53.8%) and 6 patients (50.0%) whose serum VEGF level dropped in the severe, moderate and mild group, respectively, with significant differences among these groups (p=0.046). Two year survival rates of patients with severe, moderate and mild pathologic response were 61.9%, 53.8% and 33.3% respectively, and no statistically difference between severe and mild group regarding OS (p=0.245) was tested. Conclusions: Tumor pathologic response during chemo-radiotherapy and the changes of serum VEGF lever could predict curative effects of chemo-radiotherapy in patients with esophageal cancer.

APPLICATIONS OF THE HILBERT-HUANG TRANSFORM ON THE NON-STATIONARY ASTRONOMICAL TIME SERIES

HU, CHIN-PING,CHOU, YI,YANG, TING-CHANG,SU, YI-HAO,HSIEH, HUNG-EN,LIN, CHING-PING,CHUANG, PO-SHENG,LIAO, NAI-HUI The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

The development of time-frequency analysis techniques allow astronomers to successfully deal with the non-stationary time series that originate from unstable physical mechanisms. We applied a recently developed time-frequency analysis method, the Hilbert-Huang transform (HHT), to two non-stationary phenomena: the superorbital modulation in the high-mass X-ray binary SMC X-1 and the quasi-periodic oscillation (QPO) of the AGN RE J1034+396. From the analysis of SMC X-1, we obtained a Hilbert spectrum that shows more detailed information in both the time and frequency domains. Then, a phase-resolved analysis of both the spectra and the orbital profiles was presented. From the spectral analysis, we noticed that the iron line production is dominated by different regions of this binary system in different superorbital phases. Furthermore, a pre-eclipse dip lying at orbital phase ~0:6-0:85 was discovered during the superorbital transition state. We further applied the HHT to analyze the QPO of RE J1034+396. From the Hilbert spectrum and the O-C analysis results, we suggest that it is better to divide the evolution of the QPO into three epochs according to their different periodicities. The correlations between the QPO periods and corresponding fluxes were also different in these three epochs. The change in periodicity and the relationships could be interpreted as the change in oscillation mode based on the diskoseismology model.

Knockdown of GCF2/LRRFIP1 by RNAi Causes Cell Growth Inhibition and Increased Apoptosis in Human Hepatoma HepG2 Cells

Li, Jing-Ping,Cao, Nai-Xia,Jiang, Ri-Ting,He, Shao-Jian,Huang, Tian-Ming,Wu, Bo,Chen, De-Feng,Ma, Ping,Chen, Li,Zhou, Su-Fang,Xie, Xiao-Xun,Luo, Guo-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: GC-binding factor 2 (GCF2) is a transcriptional regulator that represses transcriptional activity of the epidermal growth factor receptor (EGFR) by binding to a specific GC-rich sequence in the EGFR gene promoter. In addition to this function, GCF2 has also been identified as a tumor-associated antigen and regarded as a potentially valuable serum biomarker for early human hepatocellular carcinoma (HCC) diagnosis. GCF2 is high expressed in most HCC tissues and cell lines including HepG2. This study focused on the influence of GCF2 on cell proliferation and apoptosis in HepG2 cells. Materials and Methods: GCF2 expression at both mRNA and protein levels in HepG2 cells was detected with reverse transcription (RT) PCR and Western blotting, respectively. RNA interference (RNAi) technology was used to knock down GCF2 mRNA and protein expression. Afterwards, cell viability was analyzed with a Cell Counting Kit-8 (CCK-8), and cell apoptosis and caspase 3 activity by flow cytometry and with a Caspase 3 Activity Kit, respectively. Results: Specific down-regulation of GCF2 expression caused cell growth inhibition, and increased apoptosis and caspase 3 activity in HepG2 cells. Conclusions: These primary results suggest that GCF2 may influence cell proliferation and apoptosis in HepG2 cells, and also provides a molecular basis for further investigation into the possible mechanism at proliferation and apoptosis in HCC.