Effects of Intravenous Adminitration of Nimodipine on Cerbal Pial Vessels after Experimental Subarachnoid Hemorrhage in Rabbits

Hwang, Soon Gu,Lee, Young Woo 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.7

가토에서 대조내 자가 신선혈 0.2㎖를 주입 후 6시간에 생리식염수와 여러 농도의 운nimodipine 용액(10, 30 및 60㎍/㎏)을 각각 정맥주사하여 야기되는 뇌연막혈관들의 반응(직경변화)을 연구하였다. 혈관의 측정은 개두술 후 수술현미경 최고 확대(40×)하에서 현미경의 대안렌즈에 Filar micrometer eyepiece(424 and 426, AO)을 부착하여 시행하였다. 이 Filar micrometer eyepiece의 한 눈금은 3.4㎛로 매우 미세한 소혈관까지 직접 측정할 수 있었다. PaO₂, PaCO₂ 및 동맥혈 pH는 대개 전 실험을 통해서 의의있는 변화를 보이지 않았다. 수축기 동맥압은 nimodipine 용액 주입 직후에 다소의 하강을 보였으나(p<0.05) 이러한 변화는 수축기 동맥압의 일시적인 하강현상이고 곧 정상 수준으로 복귀했다. 실험적 뇌지주막하 출혈 6시간 후 nimodipine 용액과 동일한 량의 생리식염수를 주입하였을 때 뇌연막혈관의 반응은 미미하여 그 통계학적 의의는 없었다(p>0.05). 그러나 nimodipine 용액을 정주하였을 때는 Group Ⅰ, Group Ⅱ 그리고 Group Ⅲ 모두에서 뇌연막혈관의 확장을 보였다. 이러한 혈관의 확장은 동맥, 특히 소동맥에서 현저한 양상을 보였으며 대연막동맥에서도 상당히 의의있는 혈관 확장을 보이기는 했으나 소연막동맥에서만큼 현저하지는 않았다. 이러한 뇌 연막동맥의 확장은 약물 주입 후 5, 10분에 가장 뚜렷하였으며 그 후는 시간이 경과함에따라 혈관의 직경이 서서히 감소하는 양상을 보였다. 뇌연막정맥의 경우에도 nimodipine용액의 정주 후 미약한 정도의 혈관확장을 보였으나 그 확장된 정도는 뇌연막동맥에 비해 미미하였으며 통계학적 의의도 적거나 없었다. 뇌연막동맥의 nimodipine에 대한 반응은 10, 30 그리고 60㎍/㎏의 약물 양의 변화에 비교적 민감하여 용량에 비례하는 소견을 보였다. 이상의 결과에서 뇌 지주막하 출혈이 야기된 실험동물에 nimodipine을 정주했을 때 뇌연막동맥의 현저한 확장이 야기되며 특히 소동맥이 민감하게 확장되는 사실은 뇌지주막하출혈에 의한 뇌허혈성 질환에 있어 nimodipine의 치료적 유용성을 입증할 수 있는 좋은 증거가 될 것으로 생각되며 그 효능은 정주된 실험약물의 양에 비례하였다. 이로서 부작용이 허용되는 범위 내에서 다량의 nimodipine을 허혈성 뇌혈관질환에 사용하여 치료적인 효과를 기대할 수 있을 것이라 생각된다. The author have studied effects of intravenous injection of normal saline and nimodipine solution(10, 30 and 60㎍/㎏) on cerebral pial vessels at 6 hours after experimental subarachnoid hemorrhage. Measurement of pial vessels were performed under operation microscope through the bilateral craniectomy area. Filar micrometer eyepiece was attached on the oculus of microscope(40×) to make it easier to measure fine pial vessels directly. Measuring unit of this micrometer eyepiece was 3.4㎛. Physilogic parameters(PaO₂, blood pH) generally did not show significant change all through the experiments except that systolic blood pressure showed temporary decrease immediately after the injection of nimodipine solution, which returned to base level soon. Small pial arteries were by far most effectively dilated by intravenous injection of nimodipine solution especially in 5 minutes. Large arteries also presented significant dilatation after the injection. The arteries were dilated as dose-dependent manner. Veins showed dilatation to the lesser degree than arteries independent of dosage of nimodipine, that was statistically of no significance. Conclusively, nimodipine might be used in treatment of vasospasm in subarachnoid hemorrhage in practice.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

여자 양궁선수들의 경쟁상태불안에 대한 분석

김갑구,이순천 啓明大學校 生活科學硏究所 1998 科學論集 Vol.24 No.-

The purpose of this study was to analyze a cognitive anxiety, somatic anxiety, and self-confidence on a competitive state anxiety from 39 females of archers. The subjects of study were 16 professionals, 11 college females students, and 12 high school female student. Statistical methods of this study were used one-way ANOVA for analysing responses of subjects on each subdomains of the competitive state anxiety. The finding of this study were as the following : 1. High school student archers showed higher cognitive anxiety than professional and college archers. 2. Somatic anxiety showed higher by age to be grown older in the professional, college, and high school archers. 3. The level of self-confidence was lower in comparison of cognitive anxiety, somatic anxiety. The professional archers showed the highest self-confidence than college and high school archers.

비뇨생식기계 영역의 감염증에 있어서 Cefminox(Meicelin^(�))의 치료 효과

강순구,박만수,설종구 최신의학사 1991 最新醫學 Vol.34 No.10

PDP용 유전체의 반사특성과 유전특성

권순석,김명녕,이상래,황문구 한밭대학교 2004 한밭대학교 논문집 Vol.21 No.-

이 논문에서는 P₂O_(5)-ZnO-BaO계와 SiO₂-ZnO-B₂O₃계의 반사특성과 유전특성이 연구 되었다. P₂O_(5)-ZnO-BaO계의 반사율은 SiO₂-ZnO- B₂O₃계의 반사율보다 낮게 나타났고, TiO₂의 함유량이 증가할수록 반사율이 감소되었다. 또한 P₂O_(5)-ZnO-BaO계의 유전율은 SiO₂-ZnO-B₂O₃계의 유전율보다 높은 값을 보였고, TiO₂ 함유량이 증가함에 따라 두 계 모두 유전율이 증가되었다. 이 현상은 공간전하효과로 설명될 수 있다. 본 연구의 결과는 높은 반사율과 높은 절연파괴 강도가 요구되는 플라즈마 디스플레이(PDP)의 하판 유전체, 또는 플라즈마 현상을 응용하는 조명 및 광고용 기구의 반사 유전체로서 응용이 가능할 것으로 기대된다. In this paper, the reflectance and the dielectric characteristics for P₂O_(5)-ZnO-BaO system and SiO₂-ZnO-B₂O₃ system have been investigated as a function of contents of TiO₂. The reflectance was decreased with increasing the contents of TiO₂ contents, and the reflectance of P₂O_(5)-ZnO-BaO system was lower than that of SiO₂-ZnO-B₂O₃ system. The dielectric constant of P₂O_(5)-ZnO-BaO system was higher than SiO₂-ZnO-B₂O₃ system, and the dielectric constant in the both system was increased with increasing of TiO₂ contents. This could be explained as the space charge effects. These results are could be applied to the under plate dielectrics of PDP required high reflective ratio and breakdown strength.

비발치로 치료한 제1급 부정교합자의 재귀현상에 관한 연구 : EVALUATION ON CLASS Ⅰ NONEXTRACTION THERAPY

김구순,이기수 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

One of the strenuous problems in orthodontic procedures is postretention stability and retention against relapse. Many investigative trial had been done to disclose the factors associated with relapse and effective prescription to stave off, however, the nature Of these jeopardies remained obscure. The objective was to investigate the long-term stability and quantitative changes of dental arches subsequently after Class I nonextraction treatment. Study models,cephalometric headfilms of 26 samples which were taken before, after teatment and postretention were employed to measure the interdental width of corresponding buccal teeth,overbite,overjet and the inclination of incisors and molars. Statistical analysis was carried to compare each measurements across the time period, and followings were brought around. 1. The quantitative amount of relapse in overbite presented positive correlation with the amount of changes through the treatment 2. Stability of intercanine width was so far secure in the case the expansion had been done through. 3. The amount of changes in intercanine width of the lower regardless of expansion or constriction manifested less than the upper, however, the relapse ratio got high. 4. The upper and lower incisors were likely to be labioversive, and remained stable after retention. 5. The first molars of the upper and lower were conceivably tipped back immediately after treatment and returned to the original angulation. The expansion of intermolar width stayed stable across the time scheme after treatment It was suggested that the maintenance of intercanine width of lower was pertinent to perform the postretention stability, and the expansion of dental arch shoudbe confined within physiologic boundaries of the patients.

비발치로 치료한 제1급 부정교합자의 재귀현상에 관한 연구

김구순,이기수 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.1

이 연구는 제1급 총생치 부정교합자를 비발치로 치료한 후, 교정치료개시기에서부터 보정완료 후까지 장기간의 변화를 관찰하여 치열의 양적 변화와 그 경향을 이해하기 위하여 시행되었다. 연구자료는 26명에 대한 교정치료개시, 교정치료 직후 빛 보정종료까지의 일련의 석고모형과 두부엑스선규격사진이었으며, 이들을 계측하고 분석하여 다음의 결과와 결론을 얻었다. 1. 수직부피개의 재귀량은 치료량과 상관성이 있었다. 2. 상악견치간 폭경은 치료직후 확대된 증례에서는 안정성이 있었으나 감소된 증례에서는 재귀율이 컸다. 3. 하악견치간폭경은 치료직후 확대 혹은 축소에 관계없이 재귀율이 높았다. 4. 상하악 전치는 치료직후 순측경사를 보였고, 보정기간 동안에 안전성이 있었다. 5. 치료직후 상하악 제1대구치는 대체로 원심경사하였으며 보정기간중에 원래의 위치로 재귀하였으며, 대구치간 폭경은 치료직후 약간 확대되어 보정기간중에 안정성을 보였다. 6. 비발치 치료증례의 보정은 하악 견치간 폭경의 유지에 유의하여야 하며, 하악치열궁의 확대는 해당악골의 생리적 한계내에서 이루어져야 할 것으로 생각된다. One of the strenuous problems in orthodontic procedures is postretention stability and retention against relapse. Many investigative trial had been done to disclose the factors associated with relapse and effective prescription to stave off, however, the nature of these jeopardies remained obscure. The objective was to investigate the long-term stability and quantitative changes of dental arches subsequently after Class I nonextraction treatment. Study models, cephalometric headfilms of 26 samples which were taken before, after treatment and postretention were employed to measure the interdental width of corresponding buccal teeth, overbite, overjet and the inclination of incisors and molars. Statistical analysis was carried to compare each measurements across the time period, and followings were brought around. 1. The quantitative amount of relapse in overbite presented positive correlation with the amount of changes through the treatment. 2. Stability of intercanine width was so far secure in the case the expansion had been done through. 3. The amount of changes in intercanine width of the lower regardless of expansion or constraction manifested less than the upper, however, the relapse ratio got high. 4. The upper and lower incisors were likely to be labioversive, and remained stable after retention. 5. The first molars of the upper and lower were conceivably tipped back immediately after treatment and returned to the original angulation. The expansion of intermolar width stayed stable across the time scheme after treatment. It was suggested that the maintenance of intercanine width of lower was pertinent to perform the postretention stability, and the expansion of dental arch shoud be confined within physiologic boundaries of the patients.

316L 스테인리스강의 고온 저주기 피로저항에 미치는 동적변형시효의 영향

홍성구,이순복 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.2

Mechanism of dynamic strain aging (DSA) and its effect on the low-cycle fatigue (LCF) behavior in type 316L stainless steel were investigated by performing LCF tests in air in a wide temperature range from 20 to 650℃ with strain rates of 3.2x10^(-5)-1x10^(-2)/s. The regime of DSA was evaluated using the anomalies associated with DSA and was in the temperature range of 250-550℃ at a strain rate of 1x10/s, in 250-600℃ at 1x10^(-4)/s, and in 250-650℃ at 1x10^(-2)/s. The activation energies for each type of serration were about 0.57-0.74 times those for lattice diffusion indicating that a mechanism other than lattice diffusion is involved. It seems to be reasonable to infer that DSA is caused by the pipe diffusion of solute atoms through the dislocation core. Dynamic strain aging reduced the crack initiation and propagation life by way of multiple crack initiation, which comes from the DSA-induced inhomogeneity of deformation, and rapid crack propagation due to the DSA-induced hardening, respectively. (Received October 29, 2004)

저주기 피로변형시 316L 스테인리스강의 비안정화 거동에 미치는 온도의 영향 : 반복응력거동 및 적정 피로수명인자 Cyclic Stress Response and a Suitable Fatigue Parameter

홍성구,이순복 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.1

In this study, cold worked (CW) 316L austenitic stainless steel was investigated by performing low-cycle fatigue test with varying temperature, strain amplitude, and strain rate. CW 316L stainless steel underwent additional hardening at room temperature and in the temperature range of 250-600℃: The hardening at room temperature came from plasticity-induced martensite transformation, and the hardening over 250-600℃ was attributed to dynamic strain aging. These hardening mechanisms competed with the softening mechanism which was induced by dynamic recovery, generally observed in cold worked materials, resulting in the cyclical non-stabilization of the material. Three fatigue parameters, such as stress amplitude, plastic strain amplitude, and plastic strain energy density, were evaluated. The results revealed that plastic strain energy density was nearly invariant through a whole life and, thus, recommended as a suitable fatigue parameter. (Received September 3, 2004)