여주 건조분말 함유식이가 고콜레스테롤 혈증 Rat의 혈청성분에 미치는 영향

이웅수 忠州大學校 2007 한국교통대학교 논문집 Vol.42 No.-

This research was carried out to investigate the hypocholesterolemic effects of diets containing bitter gourd(Momordica charantia, MC; Yeoju) powder in Sprague-Dawley male rats. After 2 weeks feeding with basal diet, 6 week old rats were fed with basal diet, diets containing 1% cholesterol, and 1% MC, 5% MC and 10% MC powder with 1% cholesterol for 2, 4 and 6 week. And then I measured the levels of glucose, triglyceride, total-, HDL-and LDL-cholesterol, and the activities of GOT, GPT in the serum of rats. The levels of triglyceride and total-cholesterol in the serum of rats were decreased remarkably in the groups fed with diet contained more 5% MC powder compared to the basal diet group for 2weeks. The levels of LDL-cholesterol were decreased in the all groups fed with diet contained MC power for 4weeks, especially groups fed with contained more 5% MC powder were more effective than the other groups, but the decrease of HDL-cholesterol level was not effective in all diet groups contained MC powder. The levels of blood sugar were decreased in all MC powder diet groups, and the decreased levels were more effective in the groups fed with diet contained more MC powder. The activities of GOT and GPT were also decreased in all MC powder diet groups. In conclusion, diets contained MC powder were effective in the decrease of triglyceride, total- and LDL-cholesterol, and glucose levels, and GOT and GPT activities in the serum of rats

장내세균에 의한 Trichothecene Mycotoxins의 대사 : (I) T-2 toxin의 대사

이웅수 忠州大學校 2008 한국교통대학교 논문집 Vol.43 No.-

Trichothecene mycotoxins are a chemically related group of toxic fungal metabolites produced by a number of species of the fungi such as Fusarium, Cephalosporium, Trichothecium, Myrothecium, Stachybotrys and Trichoderma, and are responsible for mycotoxicoses as causative agents in a wide variety of animal and human health problems. Especially, T-2 toxin is a secondary metabolite produced by Fusarium spp. such as F. tricinctum, F. poae and F. sporotrichioides etc., and is often found in agricultural products and feeds including cereals, and is a potent cytotoxic and immunodepressive trichothecene mycotoxin, and causative agent of moldy corn toxicosis and alimentary toxic aleukia(ATA), and induces acute toxicity such as leukocytosis, hemorrhage and dermal edema, followed by death in animals. In order to elucidate the possible metabolism of T-2 toxin by rat and human intestinal bacteria, this research was carried out. T-2 toxin transformed into HT-2 toxin and unknown metabolite by cultured intestinal bacteria in the anaerobic condition. The amount of HT-2 toxin was decreased, but unknown metabolite was increased by subsequent incubation. This metabolite was elucidated to deepoxy HT-2 toxin by NMR and mass spectra data. It was suggested that T-2 toxin was deepoxidized after deacetylation to HT-2 toxin by the intestinal bacteria. This deepoxy HT-2 toxin was transformed into HT-2 toxin and 15-deacetyl-deepoxy HT-2 toxin by liver microsome of PCB-treated rat in the presence of NADPH and oxygen. These results suggest that the epoxide group of trichothecene mycotoxins is reduced by intestinal bacteria and re-oxidized by hepatic microsomal enzyme of rat.

설사환자의 대변에서 분리한 세균성 병원체의 특성 조사 II. Staphylococcus aureus의 분리·동정 및 항생제에 대한 내성 조사

이웅수 忠州大學校 2010 한국교통대학교 논문집 Vol.45 No.-

I investigated the characterization of bacterial pathogens on 2,270 stool specimens, which was collected from dysentery patients for one year, 2007 Detection of enteric bacteria in stool of diarrheal patient was performed according to the procedure of routine protocol such as culture and multiplex PCR method. Biochemical test for the identification of Staphylococcus aureus(S. aureus) was performed by API Staphy kit. In order to cofirm of toxin type, I also carried out serological test. Among 456 isolates of bacterial pathogen isolated from the collected stool, S. aureus was 74 isolates(toxin A type 16, C type 9, D type 1, non-toxin type 48). Detection rate of S. aureus in dysentery patients was the higher in April, August and December, and in children below 10 years old(66.2%). In addition, S. aureus in male was isolated higher rate than their in female. Antibiotics resistance rate on the each toxin type S. aureus showed that type A is AM > PC > EM, OC > TC > CP > CT > CM > GM, IP > CI > ChP > TM/SM, RP, and type C is AM, PC, EM, OC > CP, CM, TC > CT, CI, IP > GM > TM/SM, RP, and type D is only 100% on AM, PC and TC. In the present study, I focused to investigate the incidence and antibiotics resistance patterns in each toxin type S. aureus related diarrheal patients. These results were very helpful to make preventive method for infectious disease causing bacterial gastroenteritis, especially, food poisoning by enteric bacterial infection.

산업대학 건축공학과 교육과정 개발에 관한 연구

임응찬,한인웅,이승준,박재호,조방현,서형수,정구용,한기원,김진원,임상규,이재윤 三陟大學校 1996 論文集 Vol.29 No.2

By impproving the educational program of the Department of Architectural Engineering and studying the theory of learning and the applied method necessary for the development of nation and society, we should develop a satisfying course of education to meet the demand and skill in the industralized society for the purpose of cultivating men of baility with great personalities and creative minds. Therefore, this study focused on classifying several kinds of occupations in the prart of architectured related to what graduates from the department of Architectural Engineering go with into the society. Accordingly after choosing some works adequate to each occupation by the proposed curricula, we tried to establish the scope of education and courses corresponding to the jobs and thus and develop a satisfying curriculum.

여성 생식계조직의 각종 임신연관 혈장단백의 분포에 관한 면역효소조직화학적 연구

남웅수,이규완,홍성봉 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3

Pregnancy associated plasma protein-A(PAPP-A), pregnancy-specific β-1 glycoprotein (SP1) and human placental lactogen(HPL), which are specific protein, have been known to be detectable in the plasma of pregnant women, especially in mid and last trimester by radioimniunoassay. Various researches have been reported on these and the increments of them could be of value as the tumor markers and as the pregnancy related abnormalities. Surgical specimens of normal and pathologic female genital tissues were fixed in formalin and embedded in paraffin in order to detect the exact distribution of PAPP-A, SP1 and HPL in various tissue component of them. The result obtained are as follows: 1. Normal superficial and intermediate squamous epithelial cells, moderate and severe dysplasia, carcinoma in situ and keratinizing squamous cell carcinoma of uterine cervix revealed positive reaction to anti PAPP-A, SP1, and HPL antibodies. 2. Endometrium in both proliferative and secretory phase, endometrial hyperplasia, trophoblastic tumors revealed positive reaction to anti PAPP-A, SP1, and HPL antibody, hut endometrial adenocarcinoma revealed negative reaction to each of them. 3. All of the normal ovarian tissues and various epithelial ovarian tumors revealed negative reaction to PAPP-A, SP1 and HPL antibody, but stromal and germ cell ovarian tumors revealed positive reaction to each of them. 4. Reactions to each PAPP's were in uniformity among the same tissues, that is, once the reaction was positive, the reactions to the other remaining two PAPP's were positive. Similarly negative reactions appeared in the same fashion.

NPV法 計算에 減價償却費가 미치는 影響

朴秀雄 慶星大學校 1983 부산수산대학 논문집 Vol.4 No.3

Effect of Dietary Vitamin E on the Release of Iron and Generation of Mitochondrial Hydrogen Peroxide in Rat

이웅수 忠州大學校 2003 한국교통대학교 논문집 Vol.38 No.2

In this research, whether dietary vitamin E has a direct functional effect on the production of reactive oxygen species(ROS) in mitchondria, and whether dietary vitamin E influence the formation of free iron in rat tissues were determined. Additionally, if altered iron release is linked to the degree of peroxidative damage was determined. The results clearly showed that dietary vitamin E markedly reduced hydrogen peroxide production in the mitochondria of liver and skeletal muscle of both male and female rats in a dose-dependent manner. Also, the data showed that deprivation of vitamin E resulted in an increase of free iron in the skeletal muscle and spleen of male rats and liver, skeletal muscle, heart, kidney, and spleen of female rats. Supplementation with vitamin E significantly decreased the levels of free iron in the skeletal muscle of both male and female rats. Also, the levels of free iron were significantly inversely related to the concentration of supplemented vitamin E in the skeletal muscle, kidney, and spleen of male rats and liver, skeletal muscle, heart, kidney, and spleen of female rats. Deprivation of vitamin E caused a significant increase in the levels of thiobarbituric acid reactants(TBARS) in the liver, skeletal muscle, heart, kidney, and spleen of both male and female rats. Vitamin E does-dependently decreased TBARS in the liver and kidney of male rats and liver, skeletal muscle, and kidney of female rats. The results obtained from this research indicate that vitamin E regulates mitochondrial generation of ROS and attenuates the release of iron from its protein complexes. The results also suggest that the overprodution of mitochondrial ROS is the first event leading to tissue damage observed in vitamin E-deficiency syndromes and that formation of free iron is the first event of oxidative tissue damage.

중ㆍ장거리 선수의 최대산소섭취량, 무산소성 역치 및 혈액성분에 관한 연구

이영웅,박수연,박철빈 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The purpose of this paper was evaluate the specificity of maximal oxygen uptake and anaerobic threshold which is indicator of cardio- respiratory function during incremental load exercise using treadmill, also. Twenty of elite middle and long distance runners participated as subjects of this research. By measurement the specificity of cardio-fulmonary function and blood component in middle and long distance runners, the conclusion and results which is obtained is as follows: 1.Maximal oxygen uptake of absolute and relative value during maximal exercise load in middle and long distance runners was display 4.23±0.421/min & 71.2/㎖/kg/min, respectively, also, maximal ventilation and maximal heart rate was expressed 146± 18.861/min & 192.25 ± 7.61beats/min, respectively. 2.Oxygen uptake and relative percentage of maximal oxygen uptake in the anaerobic threshold levels of middle and long distance runners was shown 3.55± 0.491/min(46.54㎖/kg/min) 72.81±8.86%, respectively and ventilation, heart rate and relative percentage of maximal heart rate was expressed 95.09 ± 16.951/min, 176.54 ± 9.79beats/min and 91.62 ±3.90%, respectively. 3.Concentration of plasma lactate(t-value:-3.65, p<0.003), glucose (t-value:-4.52, p<0.02) In blood component of middle and long distance runners was display the significantly difference between pre and post value after maximal incremental exercise load.

配當과 株價에 關한 硏究 : 一部上場 企業을 中心으로

朴秀雄 경성대학교 1982 부산수산대학 논문집 Vol.3 No.1

Rat의 간세포 항산화계 및 지질 과산화에 미치는 Mycotoxins의 영향 : I. Effects of Fumonisin B₁, a Mycotoxin produced by Fusarium moniliforme I. Fumonisin B₁의 영향

李雄洙 忠州大學校 1994 한국교통대학교 논문집 Vol.29 No.2

The purpose of this study was to determine if fumonisin B₁(FB₁), a promoter for hepa tocarcinogenesis in rat, induces lipid peroxidation and alters the concentrations of antioxidant systems in rat liver cell. The hepatocytes were isolated from male Harlan Sprague-Dawley rat by using aseptic procedures, and treated with varying concentrations of FB₁(1μM,10μM and 50μM). The levels of malondialdehyde(MDA), conjugated diene(CD), vitamin A(V-A), vitamin E(V-E),γ-tocopheryl quinone(γ-TQ), vitaminC(V-C), glutathione(GSH), total lipids(TL), and protein were determined to assess oxidative damage and antioxidant status on the hepatic cell. FB₁ increased the levels of MDA and CD, oxidative products of cellular lipid. And FB₁ decreased the levels of cellular antioxdants such as V-E, and V-A, and V-C, but increased GSH level. This study shows that the adiministration of FB₁, a mycotoxin produced by Fusarium moniliforme, did increase indices of lipid peroxidation, but that cellular antioxidant defences were inhibited by FB₁.