New Zealand White 토끼의 생식세포 및 체세포 분열에 의한 염색체 분석

신선희,김희수,최영현,이원호 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-

토끼(New Zealand White rabbit)의 감수분열 및 유사분령상을 통해 그 염색체적 특징을 조사하였다. 감수분열 염색체의 표본 작성은 공기건조법을 다소 변형하여 사용하였고, 유사분열 중기상의 핵형분석에는 G-분염법을 이용하여 다음과 같은 결과를 얻었다. 1. 제1감수분열의 접합기와 태사기의 염색소립과 sex vesicles 및 동원체가 뚜렷하게 구별되었다. 태사기의 중기에서부터 후기 과정이 진행되는 동안에 2가염색체에서 측면으로 돌출한 머리카락 모양의 돌기들이 관찰되어졌고, 이 돌기들은 염색체가 lampbrush 염색체 구조의 모양이 되도록 해주었다. 이동기의 염색체들은 키아즈마의 수와 위치에 따라 분류가 가능하였다. 제1 감수분열 전기의 후반부 과정과 중기Ⅰ 시기에는 2가염색체로 된 21개의 상염색체와 1개의 말단결합으로 연결된 X-Y 염색체를 관찰할 수 있었다. 2. New Zealand White rabbit의 생식세포에서 관찰된 2가 염색체의 형태는 1CH, 1TAl 및 2TA 2가염색체 유형이 대부분을 이루었다. New Zealand White rabbit에서의 평균 키아즈마 빈도는 약 30.2로 나타났으며, 키아즈마 빈도가 복사기, 이동기 및 중기Ⅰ로 분열이 진행됨에 따라 다소 감소하는 경향이 있었다. 3. New Zealand White rabbit의 핵형분석에 의하면 염색체 수는 44개(2n=44)로서, 8쌍의 중부염색체, 9쌍의 차중부염색체, 4쌍의 단부염색체로 된 21쌍의 상염색체와 중부염색체인 1개의 X 염색체 및 단부염색체인 1개의 Y 염색체로 구성되어 있음을 알 수 있었다. Chromosomal characteristics of New Zealand White rabbit was studied at meiosis and mitosis. The meiotic chromosomal preparations were made with the modified air-drying method and karyotype analysis was performed with the G-banding technique, using isolated mitotic metaphase chromosomes of the New Zealand White rabbit. Chromosomes, sex vesicles and centromeres could be could be classified in the zygotene and the pachytene of the meiosis Ⅰ. The hair-like processes projecting laterally from the axes of bivalent chromosomes at the mid-late pachytene were basis of the numbers and the locations of chiasma in the diakinesis. Twenty-one autosomal bivalents and a single unequal terminally associated X-Y bivalent were observed during the late prophase and the metaphase of the meiosis Ⅰ. Most of the bivalent types observed in the New Zealand White rabbit spermatocytes were 1CH, 1TAl, and 2TA bivalents. The mean chiasma frequency (CE) of the male New Zealand White rabbit was 30.2, and it was found that the CF value tended to decrease through the diakinesis and the metaphase Ⅰ. The karyotype of the New Zealand White rabbit was a male chromosome number of 44(2n=44), comprising 8 pairs of metacentric, 9 pairs of submetacentric, 4 pairs of acrocentric autosomes, metacentric X chromosome and acrocentric Y chromosome.

인슐린 비의존형 당뇨병 환자에서 혈장 Endothelin-1농도의 변화

신양수,조희충,김원식,국기용,김용화,정종훈,문철웅,배학연,양성훈 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Endothelin(ET) is a 21-residue peptide originally isolated from the cultured porcine endothelial cells. There are at least three genes for endothelin:endothelin-1(ET-1), endothelin-2(ET-2), and endothelin-3(ET-3). Endothelins are present in various human biological fluids including plasma, urine, breast milk, and saliva and have been found elevated plasma ET concentrations in patients with diabetes mellitus, Patients undergoing maintenance hemodialysis due to chronic renal failure, patients with acute myocardial infarction, and patients with subarachnoid hemorrhage. Endothelial cell damage is suspected to occur in diabetic patients and may be one important cause of angiopathy, a major complication in diabetes mellitus. The elevation of ET in diabetic patients may be a marker of, and further exacerbate, their vascular disease. We measured the levels of ET-1 in plasma of 50 patients with non-insulin dependent diabetes mellitus(NIDDM) and 25 normal subjects by radioimmunoassay. The plasma ET-1 concentration (mean±S.D.) in NIDDM was 6.461 A2.510 f㏖/ ㎖, and was significantly higher than in normal subjects (4.567±1.155f㏖/㎖) (P<0.05). The plasma ET-1 concentration (mean±S.D.) in diabetic retinopathy group( 7.15±2.454 f㏖/ml) was significantly elevated than those in otherwise uncomplicated groups (5.348±2.390 f㏖/㎖)(P<0.01). The correlation between any clinical parameters and plasma ET-1 levels in NIDDM was not significant, In conclusion, this study suggest that the elevated levels of ET-1 in diabetic patients may be play a important role in the pathogenesis of diabetic complications.

소수성 촉매를 이용한 수소동위원소 교환 반응 측정을 위한 순환 반응기

최희주,이한수,안도희,강희석,김광락 한국공업화학회 2002 응용화학 Vol.6 No.2

The catalytic rate constants of hydrophobic catalysts developed for the removal of tritium mainly generated in the pressurized heavy water reactors should be measured. A recycle reactor was designed and built for characterzing the long-term behavior of the catalyst performance at the various temperatures and gas velocities.The catalytic rate constants were measured at the apparent gas velocity of 0.5 m/s and the temperature of 60℃. The initial catalytic constant was 8.44×10^-4mol/g.sec. This value is much greater than that considered in the design of Wolsong Tritium Removal Facility.

食用色素開發에 關한 硏究

鄭輝秀,陳甲德 嶺南大學校 環境問題硏究所 1984 環境硏究 Vol.4 No.2

A study for development of natural red pigment in food and drug industry was proceeded to anthocyanin from prunus yedoensis Matsum, isolated through silica gel column and further subjected to TLC. The color effect and stability from several factors was detected by spectrophotometry in 520 nm and was compared to amaranth and then 1/200 times of it. The content of the color of the fruits of Prunus yedoensis Matsum was 0.6%. As this pigment was more safe about 7.4 times in ??,but was influenced by pH(3.0-8.0) and by some metal ions (??, ??, ??, ??),and respectively stable to temperature, sun light, ultra violet ray and organic acids than amaranth, and was not given a bad infouences by sugars, recovery of another characteristics of this pigment or co-factors to compensate and promote the color effect, for example Cu(), is expected.

광합성세균 Rhodospirillum rubrum의 생장과 Bacteriochlorophyll 및 Carotenoids의 형성에 대한 탄소원의 영향

송승희,이수오,오덕철 濟州大學校 基礎科學硏究所 1994 基礎科學硏究 Vol.7 No.1

광합성 세균인 Rhodospirillum rubrum으로 생장과 색소 형성에 대한 탄소원의 영향을 조사하였다. 탄소원은 acetate, alanine, glutamate 그리고 malate였으며 단독 또는 혼합하여 사용하였다. 세포 생장은 glutamate배지에서 가장 느렸으며 glutamate-malate 혼합배지에서 가장 빨랐다. acetate와 glutamate를 혼합 하였을 때는 두 가지를 단독으로 사용했을 때보다 월등히 빠른 생장을 나타내었다. 대부분의 경우 탄소원을 단독으로 사용했을 때보다 혼합하였을 때 빠른 생장을 나타내었다. 대부분의 경우 탄소원을 단독으로 사용했을 때보다 혼합하였을 때 빠른 생장을 나타내는 것으로 보인다. 색소가 없는 R.rubrum은 혐기적으로 광을 조사하였을 때 bacteriochlorophyll과 carotenoid를 탄소원의 종류에 관계없이 동시에 형성하였으며 bacteriochlorophyll 비함량은 alanine 배지와 glutamate-alanine 혼합배지에서 가장 높고, carotenoid 비함량은 malate 배지와 malate-alanine 혼합배지에서 가장 높아서 두 색소의 비함량에 대한 탄소원의 영향은 다르게 나타났다. 탄소원에 따른 생장량과 색소 함량은 반드시 일치하지 않았고 색소 형성이나 생장에 있어서의 효과는 탄소원의 종류에 따라 달랐다. 세포내 bacteriochlorophyll에 대한 carotenoid의 비함량비는 다소 변화가 있으나 대개 1: 1 수준이었다. The influences of organic compounds such as acetate, alanine, glutamate and malate, on the growth and formation of photosynthetic pigments as a sole or mixed carbon sources were tested in a photosynthetic bacterium, Rhodospirillum rubrum. Cells grew most rapidly in culture on glutamate-malate mixtrues and showed slowest growth in glutamate among all cultures.Cells cultured on acetate-glutamate mixtures showed much rapid growth than those on single carbon source separately. Cells cultured on mixed carbon sources generally grew more rapidly than those on each single carbon source, so it seems that mixtures of carbon sources exhibit synergistic effect on cell growth. The for mation of bacteriochlorophyll and carotenoids was initiated simultaneously regardless of the kind of carbon source. The synergistic effect of mixed carbon sources on the formation of photosynthetic pigments of R. rubrum was not shown distinctly. The influence of carbon sources on the growth and formation of photosynthetic pigments were not coincided. The ratios of specific contents of carotenoids to bacteriochlorophyll were about 1 : 2 with some variations according to carbon sources.

다짐점토의 간극수압 모델에 관한 연구

임희대,박수용 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.1

The behavior of an unsaturated soil tested under undrained loading conditions depends on the magnitude of the pore-air and pore-water pressure developed. This paper derives pore pressure parameters that can be utilized in determining the pore pressure response of the compacted clayey materials. Pore pressure parameter expressions are also derived for the pore-water pressures induced as a result of isotropic stress changes and deviator stress changes during undrained loading. The parameters required to computation in the proposed model can be readily determined from the conventional triaxial compression tests. To examine the degree to which the proposed pore pressure model reflects the results of triaxial tests, The back analyses carried out, It is shown from the examination that agreements are generally satisfactory.

ABUTMENT의 경사가 CORE의 응력-변형률에 미치는 영향

임희대,박수용,우원재 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.1

Cracking of core presents a serious hazard resulting in possible leakage and failure. For a realistic evaluation of cracking of core one must take into account irregular steep abutment. The present paper is concerned with the most dangerous transverse movements in the embankment which result from non-uniform settlement along the abutments. In this study, the effect of steepness of abutment has been analyzed for 100m high central trapezodial cores with abutment slopes of 1:0.25, 1:0.5, 1:0.75, and 1:1 respectively. Numerical analysis of longitndinal sections of the embankment has been carried out by the application of finite element method. The nonlinear and stress-dependent stress-strain properties of soil is approximated by using a hyperbolic model. The results of the analyses indicate that there is a decrease of vertical stress due to arching action. This decrease is more for steeper slope than for flatter slope.

주택의 물 사용 공간계획을 위한 주문화 연리 : 조리ㆍ세탁ㆍ목욕 행위를 중심으로

조성희,박수빈 대한건축학회 2004 대한건축학회논문집 Vol.20 No.9

The purpose of this research is to find cultural design principles through the meaning and use of housing in regard to cooking, washing, and bathing. A historical and behavioral study was performed to link the past and the present The meaning and use of places for cooking, washing, and bathing were grasped by literature review In order to figure out principles of space organization for those activities, a questionnaire survey was Carried out The subjects were 646 residents who live in apartments In Busan The residents' attitudes on these Issues were investigated and analyzed The main findings are as follows (1) The kitchen, as a major place for cooking, becomes a center of dwelling space by facing the livingroom The role of the kitchen as a place for eating has become weighty (2) In the past, washing the clothing was carried out outside of the house because It was considered dirty After modernization, It was not easy to place laundry facilities Within the house Today, the design of apartments for household chores needs to be developed according to meaning and use of those spaces (3) Traditionally, bathing was considered to be clean After modernization, the bathroom appeared as a room for bath10g m a house At present, the bathroom becomes a space not only for bathing but also for taking a rest (4) Principles of space organization related to water usage 10 housing were Identified m the classes of dirty/clean, and of edible/not edible

고리 및 영광 논토양에 대한 ^137Cs의 모의 사고 침적시 토양-벼 전이계수

최용호,임광묵,박효국,최희주,이한수 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.4

고리 및 영광 원전 반경 5km 내 11곳의 논토양을 원상대로 재배상자에 채취하여 실험 온실로 운반한 후 벼의 이식 전과 생육중에 ^137Cs을 담수의 표면에 처리하였다. 이식전 처리시 ^137Cs의 토양-작물체 전이계수(m²kg^-1-dry plant)는 토양에 따라 8배 정도의 변이를 보였고 평균은 지역간 차이가 거의 없이 쌀알의 경우 1.1 x 10^-4 , 볏짚의 경우 2.7 x 10^-4 이었다. 분얼초기 처리와 수잉기 처리시 전이계수는 이식전 처리에 비해 대체로 2∼3 배 및 15∼30 배 정도 높았다. 토양 유기물 함량과 전이계수 간에는 정의 상관이 있었다. 본 실험결과에 입각하여 우리나라 논에 대한 일회성 사고침적시 적용할 수 있는 ^137Cs 전이계수의 대표치를 침적시기별로 제안하였다. Undisturbed soil blocks were taken into culture boxes from 11 paddy fields within 5 km radii of Kori and Yonggwang NPPs and carried to a greenhouse where ^137Cs was applied to the surface of standing water before rice transplanting and during its growth. Transfer factors (m²kg^-1-dry plant) of ^137Cs applied before transplanting varied with soils by a factor of about 8. The mean was 1.1 x 10^-4 for hulled rice seeds and 2.7 x 10^-4 for straw without significant regional differences. The ^137Cs applications at the early tillering stage and booting stage resulted in, on the whole, 2∼3 and 15∼30 times, respectively, higher transfer factors than the application before transplanting. A positive correlation was found between soil organic matter and transfer factor. Generic values of transfer factors were proposed for an acute accidental deposition of ^137Cs onto Korean paddy fields in different times of the year.

Killer 효모 Saccharomyces cerevisiae B15-1의 에탄올 발효특성

이창호,우철주,이종수,정기택,박희동 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

정치배양에 의한 Saccharomyces cerevisiae B15-1의 에탄올 발효에 미치는 영향 인자와 초기 기질농도에 따른 발효 특성을 연구, 검토하여 에탄올 생성 최적 조건하에서 발효액중의 생성된 에탄올 농도, 균체 농도 및 당 농도와의 관계를 규명하고자 하였다. 에탄올 생성의 최적 조건은 배양 온도 30℃, 초기 pH 5.0이었으며, 에탄올 비생성 속도는 초기 기질농도가 150 g/l일 때 1.203 g-EtOH/g-cell·hr로서 가장 높게 나타났다. 에탄올 발효능은 기질농도가 150 g/l 이하에서는 거의 일정하였으나 200 g/l에서는 급격히 감소하였다. 최종 균체량과 최종 에탄올 생성량은 초기 기질의 농도가 증가함에 따라 증가하는 현상을 보였으나 그 증가율은 기질의 농도가 높을 수록 다소 감소하는 경향을 나타내었다. 초기 기질농도가 150 g/l 이하에서는 대부분이 발효에 의해 에탄올로 전환되었으나 200 g/l의 당을 사용한 경우에는 균체의 증식과 발효가 완료된 후에도 약 10.7 g/l의 기질이 에탄올로 전환되지 못하고 배지에 남아 있었다. 초기 기질 농도를 매개변수로 생성된 에탄올과 에탄올 수율을 조사한 결과 에탄올 수율은 초기 당농도가 증가할수록 급격히 감소하였으며, 초기 당농도가 일정한 경우 에탄올 생성량이 소량일 때는 에탄올의 생성량이 증가할수록 에탄올의 수율은 급격히 증가하다가 점차 완만한 증가현상을 보였으며 에탄올 생성량이 60 g/l 이상에서는 거의 일정하였다. Characteristics of ethanol fermentation were investigated during the stationary culture of a killer yeast, Saccharomyces cerevisiae B15-1. Specific ethanol production rate reached the maximum level, 1.203 g-EtOH/g-cell·hr, at 150 g/l of the initial glucose concentration. No big differences were obtained in ethanol fermentability based on the initial sugar concentration below 150 g/l. When 200 g/l of sugar was used, fermentability dropped significantly. Although the final cell mass and the amount of ethanol produced were increased, their increase rates were declined according to the increase of initial sugar concentration. It was found that most of the sugar used below 150 g/l of concentration could be changed to ethanol. However, when 200 g/l of sugar was used, some of them remained in the media even after increase of cell mass and fermentation stopped. The ethanol yield was decreased when initial sugar concentration was high, and were increased when the amount of ethanol produced was increased and finally reached the plateau over 60 g/l of ethanol concentration.