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Screening of aldose reductase inhibitory activity of white-color natural products
목소연,신현철,이상현,Mok, So-Youn,Shin, Hyun-Cheol,Lee, Sang-Hyun Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.1
The purpose of this study was to evaluate the potential of naturally occurring aldose reductase (AR) inhibitors from white-color natural products (Aruncus dioicus var. kamtschaticus, Chionanthus retusa, Cosmos bipinnatus, Hibiscus syriacus, Hydrangea paniculata, Magnolia denudata, Prunus padus, Robinia pseudo-accacia, Rhododendron mucronulatum for. albiflorum, Spiraea blumei, and Spiraea prunifolia var. simpliciflora). The MeOH extract of white-color natural products were tested on rat lens AR inhibition in vitro. Among them, the MeOH extract of R. mucronulatum for. albiflorum showed highest inhibition on AR ($IC_{50}$ value, 1.07 ${\mu}g/ml$). These results suggested that R. mucronulatum for. albiflorum, a white-color natural product, could be a useful resource in the development of a novel AR inhibitory agent against diabetic complications.
Screening of aldose reductase inhibitory activity of white-color natural products
So-Youn Mok(목소연),Hyun Cheol Shin(신현철),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.1
The purpose of this study was to evaluate the potential of naturally occurring aldose reductase (AR) inhibitors from white-color natural products (Aruncus dioicus var. kamtschaticus, Chionanthus retusa, Cosmos bipinnatus, Hibiscus syriacus, Hydrangea paniculata, Magnolia denudata, Prunus padus, Robinia pseudo-accacia, Rhododendron mucronulatum for. albiflorum, Spiraea blumei, and Spiraea prunifolia var. simpliciflora). The MeOH extract of white-color natural products were tested on rat lens AR inhibition in vitro. Among them, the MeOH extract of R. mucronulatum for. albiflorum showed highest inhibition on AR (IC50 value, 1.07 ㎍/ml). These results suggested that R. mucronulatum for. albiflorum, a white-color natural product, could be a useful resource in the development of a novel AR inhibitory agent against diabetic complications.
Determination of hesperidin in mixed tea by HPLC
한샘,목소연,김혜민,이정민,이동구,이숙영,김종기,김선주,이상현,Han, Saem,Mok, So-Youn,Kim, Hye-Min,Lee, Jeong-Min,Lee, Dong-Gu,Lee, Sook-Young,Kim, Jong-Kee,Kim, Sun-Ju,Lee, Sang-Hyun Institute of Agricultural Science 2011 Korean Journal of Agricultural Science Vol.38 No.2
The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 nm). The How rate was kept constant at 1.0 ml/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 mg/g, respectively).
Determination of hesperidin in mixed tea by HPLC
Saem Han(한샘),So-Youn Mok(목소연),Hye-Min Kim(김혜민),Jeong-Min Lee(이정민),Dong Gu Lee(이동구),Sook-Young Lee(이숙영),Jongkee Kim(김종기),Sun-Ju Kim(김선주),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2
The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 ㎚). The How rate was kept constant at 1.0 ㎖/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 ㎎/g, respectively).
최지연 ( Ji Yeon Choi ),김혜민 ( Hye Min Kim ),목소연 ( So Youn Mok ),최경 ( Kyung Choi ),구자정 ( Ja Jung Ku ),박광우 ( Kwang Woo Park ),조은주 ( Eun Ju Cho ),이상현 ( Sang Hyun Lee ) 한국응용생명화학회(구 한국농화학회) 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.3
The aim of this research was to investigate the industrialapplication of Sedum sarmentosum. Antibacterial activities of then-hexane, methylene chloride (MC), ethyl acetate, and n-butanolfractions of S. sarmentosum were tested against Escherichia coli,Staphylococcus aureus, and Helicobacter pylori. The MC fractionshowed the strongest antibacterial activity against E. coli, with aninhibition zone greater than 13mm in disc assays. At 100ug/mL all fractions scavenged more than 50% of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (·OH). In particular,the MC fraction showed the strongest scavenging activity against DPPH and ·OH. In addition, we found that treatment with the MC fraction inhibited the growth of H. pylori and gastric adenocarcinomacells. The present results suggest that the MC fraction of S sarmentosum would play the promising protective role againstpathogenic bacteria and free radicals.