Midazolam Induces Cellular Apoptosis in Human Cancer Cells and Inhibits Tumor Growth in Xenograft Mice

Siddhartha Kumar Mishra,김환묵,강주희,이창우,오승현,류준선,배윤수 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.3

Midazolam is a widely used anesthetic of the benzodiazepine class that has shown cytotoxicity and apoptosis-inducing activity in neuronal cells and lymphocytes. This study aims to evaluate the effect of midazolam on growth of K562 human leukemia cells and HT29 colon cancer cells. The in vivo effect of midazolam was investigated in BALB/ c-nu mice bearing K562 and HT29 cells human tumor xenografts. The results show that midazolam decreased the viability of K562 and HT29 cells by inducing apoptosis and S phase cell-cycle arrest in a concentration-dependent manner. Midazolam activated caspase-9, capspase-3 and PARP indicating induction of the mitochondrial intrinsic pathway of apoptosis. Midazolam lowered mitochondrial membrane potential and increased apoptotic DNA fragmentation. Midazolam showed reactive oxygen species (ROS) scavenging activity through inhibition of NADPH oxidase 2 (Nox2) enzyme activity in K562 cells. Midazolam caused inhibition of pERK1/2 signaling which led to inhi-bition of the anti-apoptotic proteins Bcl-XL and XIAP and phosphorylation activation of the pro-apoptotic protein Bid. Midazolam inhibited growth of HT29 tumors in xeno-graft mice. Collectively our results demonstrate that midazolam caused growth inhibition of cancer cells via activation of the mitochondrial intrinsic pathway of apoptosis and inhibited HT29 tumor growth in xenograft mice. The mechanism underlying these effects of midazolam might be suppression of ROS production leading to modulation of apoptosis and growth regulatory proteins. These findings present possible clinical implications of midazolam as an anesthetic to relieve pain during in vivo anticancer drug delivery and to enhance anticancer efficacy through its ROS-scavenging and pro-apoptotic properties.

LCB: Light Cipher Block An Ultrafast Lightweight Block Cipher For Resource Constrained IOT Security Applications

( Siddhartha Roy ),( Saptarshi Roy ),( Arpita Biswas ),( Krishna Lal Baishnab ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.11

In this fast-paced technological world, the Internet of Things is a ground breaking technology which finds an immense role in the present electronic world which includes different embedded sensors, devices and most other things which are connected to the Internet. The IoT devices are designed in a way that it helps to collect various forms of data from varied sources and transmit them in digitalized form. In modern era of IoT technology data security is a trending issue which greatly affects the confidentiality of important information. Keeping the issue in mind a novel light encryption strategy known as LCB is designed for IoT devices for optimal security. LCB exploits the benefits of Feistel structure and the architectural benefits of substitution permutation network both to give more security. Moreover, this newly designed technique is tested on (Virtex-7) XC7VX330T FPGA board and it takes much little area of 224 GE (Gate Equivalent) and is extremely fast with very less combinational path delay of 0.877 ns. An in-depth screening confirms the proposed work to promise more security to counter cryptographic attacks. Lastly the Avalanche Effect (AE) of LCB showed as 63.125% and 63.875% when key and plaintext (PT) are taken into consideration respectively.

Effectiveness of pre-injection use of cryoanesthesia as compared to topical anesthetic gel in reducing pain perception during palatal injections: a randomized controlled trial

Siddhartha Rai,Mehul Rajesh Jaisani,Ashok Dongol,Pradeep Acharya,Anjani Kumar Yadav 대한치과마취과학회 2024 Journal of Dental Anesthesia and Pain Medicine Vol.24 No.2

Background: Palatal injections are often painful. We aimed to compare topical ice and 20% benzocaine gel for pre-injection anesthesia before greater palatine nerve block (GPNB) injections. Methods: A randomized split-mouth clinical trial was conducted among patients aged 15-60-years needing bilateral GPNB injections. A total of 120 palatal sites from 60 patients were randomly allocated to Group A (topical ice) or Group B (20% benzocaine gel). Pain was evaluated using sound, eye, motor (SEM), and the visual analog scale (VAS) in both groups. Inferential analysis was performed using the Mann-Whitney U test. Results: The mean age of the participants was 20.5 ± 3.9 years. The median VAS score for group A was 11 (Q1 - Q3: 5.25 - 21.75), which was slightly higher than the 10 (Q1 - Q3: 4.0 – 26.75) reported in group B. However, the difference was not statistically significant (P = 0.955). The median SEM score for group A and group B was 3.5 (Q1 - Q3: 3.0 – 4.0) and 4.0 (Q1 - Q3: 3.0 - 4.0), respectively, which was statistically insignificant (P = 0.869). Conclusion: Using ice as a form of topical anesthetic for achieving pre-injection anesthesia before GPNB was as effective as 20% benzocaine gel.

Structure and Properties of Conducting Poly(o-phenylenediamine) Synthesized in Different Inorganic Acid Medium

Siddhartha Samanta,Poulomi Roy,Pradip Kar 한국고분자학회 2016 Macromolecular Research Vol.24 No.4

Poly(o-phenylenediamine) was chemically synthesized from the monomer o-phenylenediamine in various inorganic acid medium, viz., hydrochloric (HCl), sulphuric (H2SO4) and phosphoric acid (H3PO4) to investigate the differences in structures and properties of the synthesized polymers. From the various characterizations, it was confirmed that the ladder type polymer with some open ring structure was formed in HCl medium while open ring type amine derivative of polyaniline structure was obtained in H2SO4 medium and the ladder type dimer was formed in H3PO4 medium. The structural differences of the polymers were correlated with the polymerization mechanism in different acid medium. Thus, the polymer synthesized in H2SO4 was well soluble in polar organic solvent like dimethyl sulfoxide (DMSO), N,N-dimethyl formamide, tetrahydrofuran etc. and casting of freestanding film was possible from DMSO solution. The highest average DC conductivity of H2SO4 doped polymer synthesized in H2SO4 medium was observed as 7.14×10-4 S/cm. The conductivity and doping properties of the polymer were explained here from the proper characterizations.

Osteogenic Nanofibrous Coated Titanium Implant Results in Enhanced Osseointegration: In Vivo Preliminary Study in a Rabbit Model

Siddhartha Das,Sandeep Gurav,Vivek Soni,Arvind Ingle,Bhabani S. Mohanty,Pradip Chaudhari,Kiran Bendale,Kanchan Dholam,Jayesh R. Bellare 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.2

A titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and hence results in enhanced osseointegration of the implant. The osteogenic nanofibrous coating (ONFC) consists of poly-caprolactone, gelatin, nano- sized hydroxyapatite, dexamethasone, ascorbic acid and beta-glycerophosphate. ONFC exhibits optimum mechanical properties to support mesenchymal stem cells and steer their osteogenic differentiation. ONFC was subjected to various characterization tests like scanning electron microscopy, Fourier-transform infrared spectroscopy, x-ray diffractometry, thermal degradation, biomineralization, mechanical properties, wettability and proliferation assay. In pre-clinical animal trials, the coated implant showed enhanced new bone formation when placed in the tibia of rabbit. This novel approach toward implant bone integration holds significant promise for its easy and economical coating thus marking the beginning of new era of electrospun osteogenic nanofibrous coated bone implants.

Rayleigh waves in porous orthotropic medium with phase lags

Siddhartha Biswas 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.80 No.3

The present article deals with the propagation of Rayleigh surface waves in homogeneous orthotropic medium. This thermoelastic problem is studied under the purview of three-phase-lag model of hyperbolic thermoelasticity in the presence of voids. The normal mode analysis is employed to obtain a vector matrix differential equation which is then solved by eigenvalue approach. The frequency equations for different cases are derived. In order to illustrate the analytical developments, the numerical solution is carried out and the computer simulated results in respect of phase velocity and attenuation coefficient are presented graphically. Phase velocity and attenuation coefficient decreases in the presence of voids. The present problem is the most general one as other problems can be obtained as special cases from it.

Biomechanical Analysis of Sliding Board for Developing Augmented Reality Based Feedback Skating Simulator

Siddhartha Bikram Panday(시다르타),Kitae Kim(김기태),Jong-hyun Yang(양종현),Je-Heon Moon(문제헌),Do-Hoon Koo(구도훈) 한국산학기술학회 2015 한국산학기술학회 학술대회 Vol.2015 No.1

Track sports such as “Short track” and “Speed skating” are some of the winter sporting discipline dominated by South Korea which have high hopes for the upcoming 2018 Pyeongchang Olympics. Despite the winter sports being in the limelight, training of the seasonal sports are not only problematic due to accessibility but also because of financial reasons due to which, dry land training such as slide board exercise has been considered by coaches to enhance endurance, agility, balance, body composition, muscular strength, proprioception, as well as cardiorespiratory fitness. Although the exercise was initially developed for sports specific off the ice training, slide board training has been also effective for recreational purposes, conditioning, functional rehabilitation for ACL injuries as well as activities involving lateral movement. Similarly, an increased demand of sports oriented fitness equipment’s and simulators have been seen in the field of sports however, without substantial biomechanical evidence as well as interactive visual feedback of performance to the user. Hence, the purpose of the study was to provide quantitative data of skating maneuver performed on sliding board for developing augmented reality based feedback skating simulator.

Minimal clinically important difference of commonly used patient-reported outcome measures in total knee arthroplasty: review of terminologies, methods and proposed values

Maredupaka Siddhartha,Meshram Prashant,Chatte Manish,Kim Woo Hyun,Kim Tae Kyun 대한슬관절학회 2020 대한슬관절학회지 Vol.32 No.-

Purpose: The aim of this article was to highlight various terminologies and methods of calculation of minimal clinically important difference (MCID) and summarize MCID values of frequently used patient-reported outcome measures (PROMs) evaluating total knee arthroplasty (TKA). Materials and methods: PubMed and EMBASE databases were searched through May 2019. Of 71 articles identified, 18 articles matched and underwent a comprehensive analysis for terminologies used to indicate clinical significance, method of calculation, and reported MCID values. Results: MCID was the most common terminology (67% studies) and anchor-based methods were most commonly employed (67% studies) to calculate it. The analytical methods used to calculate and the estimated values of MCID for clinical use are highly variable. MCID values reported for WOMAC scores are 20.5 to 36.0, 17.6 to 33.0 and 12.9 to 25.0 for pain, function and stiffness sub-scales, respectively, and 4.7 to 10.0 for OKS. Conclusion: There was lack of standardization in the methodology employed to calculate MCID in the available studies. MCID values reported in this review could be used for patients undergoing TKA, although caution is advised in their interpretation and application.

Towards Identifying Evolving Mathematical Creativity in School Children : A Conceptual Framework

( Chattopadhyay Siddhartha Sankar ),( Sinha Dilip Kumar ) 한국수학교육학회 2011 수학교육 학술지 Vol.2011 No.-

As the title implies, the paper aims at seeking modes for possible identification of moments of mathematical creativity in its ebb and flow among gifted school children, following which a conceptual framework is aimed at. An individualistic consideration from a wider standpoint, a perception of mathematical creativity is studiedly sought for in this work. Possible layers of creativity in several classroom milieus are also looked for. Deciphering or even noticing mathematical creativity is also undertaken. The coupling from an experiential point of view is presented. Creativity ratios, obviously, in the field of mathematics are presented so that creative incidents or events can be well focused. That mathematical creativity can be well sustained forms a major interest of this paper. Nonlinearity stemming essentially from coupling in the experiential arena is also discussed. Evolving forms of mathematical creativity become discernible through the perceptual space about creativity. Few designs and patterns emerge from such dynamical exercises on mathematical creativity.

Midazolam induces cellular apoptosis in human cancer cells and inhibits tumor growth in xenograft mice

Mishra, Siddhartha Kumar,Kang, Ju-Hee,Lee, Chang Woo,Oh, Seung Hyun,Ryu, Jun Sun,Bae, Yun Soo,Kim, Hwan Mook Springer-Verlag 2013 Molecules and cells Vol.36 No.3