동충하초 균사체 및 동충하초 균사체로부터 분리된 코디세핀의 항암 활성 평가

하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),이동환 ( Dong Hwan Lee ),최민서 ( Min Seo Choe ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1

Various medicinal fungi have been traditionally used in many countries for health maintenance, as well as for the prevention and treatment of various diseases. Dong-Chong-Xia-Cha in Chinese, which translates as “winter worm and summer grass”, is an entomogenous fungus that colonized the larvae or pupae of insect. Although various biological activities of crude extracts have been reported, the precise active compounds responsible for the anticancer activities of cultured Cordyceps are not well defined. Therefore, as part of our study program to identify potential anticancer agents from traditional medicines, we used cordycepin isolated from Cordyceps militaris obtained by submerged cultivation. The aim was to isolate the pure cordycepin from the extracts of Cordyceps militaris and evaluate its anticancer properties. Silica gel column chromatographic purification of Cordyceps militaris extracts resulted in the isolation of cordycepin. The isolated cordycepin and crude cordyceps were examined for their anti-proliferative effects on human cancer cell lines, HCT, MC5-7, U-87MG, AGS, A549, and MCF-7 cells were analyzed. The isolated cordycepin displayed potent growth inhibition on HCT, MC5-7, U-87MG, AGS, A549, and MCF-7 cells with IC50 values of 86.16, 28.13, 282.40, 78.41, 137.40, and 216.40 μg/ml, respectively. A similar inhibitory trend in these cancer cells was observed for crude cordyceps, with IC50 values ranging from 60.8 317.2. The present study provides scientific supporting information for the ethnopharmacological use of Cordyceps militaris as an anticancer agent.

Evaluation of the Efficacy and Safety of DA-9601 versus Its New Formulation, DA-5204, in Patients with Gastritis: Phase III, Randomized, Double-Blind, Non-Inferiority Study

Choi, Yoon Jin,Lee, Dong Ho,Choi, Myung-Gyu,Lee, Sung Joon,Kim, Sung Kook,Song, Geun Am,Rhee, Poong-Lyul,Jung, Hwoon-Yong,Kang, Dae-Hwan,Lee, Yong Chan,Lee, Si Hyung,Choi, Suck Chei,Shim, Ki-Nam,Seol, KOREAN ACADEMY OF MEDICAL SCIENCE 2017 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.32 No.11

<P>This study compared the efficacy of DA-9601 (Dong-A ST Co., Seoul, Korea) and its new formulation, DA-5204 (Dong-A ST Co.), for treating erosive gastritis. This phase III, randomized, multicenter, double-blind, non-inferiority trial randomly assigned 434 patients with endoscopically proven gastric mucosal erosions into two groups: DA-9601 3 times daily or DA-5,204 twice daily for 2 weeks. The final analysis included 421 patients (DA-5204, 209; DA-9601, 212). The primary endpoint (rate of effective gastric erosion healing) and secondary endpoints (cure rate of endoscopic erosion and gastrointestinal [GI] symptom relief) were assessed using endoscopy after the treatment. Drug-related adverse events (AEs), including GI symptoms, were also compared. At week 2, gastric healing rates with DA-5204 and DA-9601 were 42.1% (88/209) and 42.5% (90/212), respectively. The difference between the groups was −0.4% (95% confidence interval, −9.8% to 9.1%), which was above the non-inferiority margin of −14%. The cure rate of gastric erosion in both groups was 37.3%. The improvement rates of GI symptoms with DA-5204 and DA-9601 were 40.4% and 40.8%, respectively. There were no statistically significant differences between the two groups in both secondary endpoints. AEs were reported in 18 (8.4%) patients in the DA-5204 group and 19 (8.8%) in the DA-9601 group. Rates of AE were not different between the two groups. No serious AE or adverse drug reaction (ADR) occurred. These results demonstrate the non-inferiority of DA-5204 compared to DA-9601. DA-5204 is as effective as DA-9601 in the treatment of erosive gastritis. Registered randomized clinical trial at ClinicalTrials.gov (NCT02282670)</P>

A Study on Improvement and Degradation of Si/SiO₂ Interface Property for Gate Oxide with TiN Metal Gate

Lee, Byung-Hyun,Kim, Yong-Il,Kim, Bong-Soo,Woo, Dong-Soo,Park, Yong-Jik,Park, Dong-Gun,Lee, Si-Hyung,Rho, Yong-Han The Korean Institute of Electrical and Electronic 2008 Transactions on Electrical and Electronic Material Vol.9 No.1

In this study, we investigated effects of hydrogen annealing (HA) and plasma nitridation (PN) applied in order to improve $Si/SiO_2$ interface characteristics of TiN metal gate. In result, HA and PN showed a positive effect decreasing number of interface state $(N_{it})$ respectively. After FN stress for verifying reliability, however, we identified rapid increase of $N_{it}$ for TiN gate with HA, which is attributed to hydrogen related to a change of $Si/SiO_2$ interface characteristic. In contrast to HA, PN showed an improved Nit and gate oxide leakage characteristic due to several possible effects, such as blocking of Chlorine (Cl) diffusion and prevention of thermal reaction between TiN and $SiO_2$.

A Study on Improvement and Degradation of Si/SiO2 Interface Property for Gate Oxide with TiN Metal Gate

이병현,Yong-Il Kim,Bong-Soo Kim,Dong-Soo Woo,Yong-Jik Park,Dong-Gun Park,Si-Hyung Lee,Yonghan Rho 한국전기전자재료학회 2008 Transactions on Electrical and Electronic Material Vol.9 No.1

In this study, we investigated effects of hydrogen annealing (HA) and plasma nitridation (PN) applied in order to improve Si/SiO₂ interface characteristics of TiN metal gate. In result, HA and PN showed a positive effect decreasing number of interface state (Nit) respectively. After FN stress for verifying reliability, however, we identified rapid increase of Nit for TiN gate with HA, which is attributed to hydrogen related to a change of Si/SiO₂ interface characteristic. In contrast to HA, PN showed an improved Nit and gate oxide leakage characteristic due to several possible effects, such as blocking of Chlorine (Cl) diffusion and prevention of thermal reaction between TiN and SiO₂.

일본잎갈나무 낙엽의 추출성분 및 항산화활성

사전령,권동주,김진규,황병호,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

일본잎갈나무 낙엽 (8.5kg)을 채취하여 9.5% EtOH 용액으로 추출하고 농축된 추출용액은 분획깔때기로 헥산, 메틸렌클로라이드, 에틸아세테이트 및 수용성으로 순차 추출하여 동결건조하였다. 에틸아세테이트용성과 수용성 분획에 대하여 칼럼크로마트크래피를 실시하였고, 충진물질로는 Sephadex LH-20을, 용리용매로는 메탄올 수용액 및 에탈올-핵산 혼합용액을 사용하였다. 단리된 화합물들은 TLC로 확인한 후 NMR 스펙트럼을 사용하여 정확한 구조규명을 하였고 FAB-MS와 EI-MS 스펙트럼으로 분자량을 측정하였다. 일본잎갈나무 낙엽으로부터 5개의 화합물을 단리하였으며, 각 분획물과 단리된 화합물들은 DPPH 라디칼 소거법을 이용하여 항산화 시험을 실시하였다. Fallen neeldes (8.5kg) of Larix kaempferi were separately collected. extracted with 95% EtOH. EtOH extract was evaporated under reduced pressure, concentrated then successively fractionated with a series of hexane. methylene chloride, ethylaectate and water on a separatory funnel. Then, each fraction was freeze dried. A portion of ethylacetate and water soluble powder were packed on a column chromatography (Sephadex LH-20) eluting with aqueous MeOH and EtOH-hexane mixture. Spectrometric analyses such as NMR and FAB-MS including TLC were performed to characterize the structures of isolated compounds.

오동나무 잎의 페놀성 화합물

사전령,김진규,권동주,배영수 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

강원대학교 구내림에서 오동나무 잎을 채취하여 아세톤-물(7:3, v/v)로 추출하고 n-hexane, methylene chloride, ethylacetate 및 H_(2)O 등 네 개의 분획으로 분리한 후 에틸아세테트용성 분획물을 Sephadex LH-20으로 칼럼 크로마토그래피를 수행하였으며 용리용매는 메탄올 수용액과 에탄올-핵산 혼합액을 사용하였다. 그 결과 flavonoid류 화합물인( +)-catechin, ( - )-epicatechin, apigenin, luteolin, taxifolin과 phenolic acid류 화합물인 p-hydroxybenzoic acid, vanillic acid 및 p-coumaric acid를 단리 하였다. 단리된 화합물은 NMR 및 MS 스펙트럼을 이용하여 구조를 결정하였다. The leaves of Paulownia Coreana Uyeki were collected, extracted with acetone-H_(2)O(7:3, v/v), fractionated with n-hexane, methylene chloride and ethylacetate, and freeze dried to give some dark brown powder. The ethylacetate soluble mixture was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol-hexane mixture as eluents. Spectrometric analysis such as NMR and MS From the ethylacetate fraction, five flavonides and three phenolic acids were isolated and determined.

대장내시경 후 발생한 흉부 피하기종

김시욱, 이택구, 이동욱, 배진호 충북대학교 의과대학 충북대학교 의학연구소 2011 忠北醫大學術誌 Vol.21 No.2

-본문참고-

일부 농촌지역의 모자보건 실태에 관한 조사연구

최시영,이동배,조영채 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

This study was conducted to obtain the information on maternal and child health in Wonju-Gun, Kangwondo from March 1, 1990 to April 31, 1990. The Author surveyed a total 178 women experienced child born after 1987. The results were as follows: 1. The proportion of receiving prenatal care was 95.5%. The mean times of visiting medical facilities was 4.8 and the starting time of prenatal care was 3.3 months of gestational age. 2. The proportion of hospital or clinic delivery was 69.1 % but that of home delivery was 15.2% 3. The proportion of receiving postnatal care was 26.4% and the hospital or clinics was the most common place of visit. 4. The proportion of complete vaccination for child was 77.5% and the proportion of family who use contraception was 74.2%. 5. Variables such as education period, number of child and age at marrage had high relation with the variables of maternal and child health.

저신장을 주소로 내원한 환아에서 발견된 기질성 원인 질환에 대한 임상적 고찰

고시환,이동환 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

Background: Growth is a sensitive index, which reflects the health status, nutrition and hereditary background of children. If a growth pace deviates from the normal, it could be a warning that represent s congenital or secondary diseases. A lot of research and clinical have been done since Raben reported the treatment of dwarfism using growth hormone for the first time in 1958. Recently, the interest in the growth is more and more increasing, which results in a lot of request. It is usually known that it is rare most children with short stature have abnormality in other internal organs except endocrine or congenital problems . Sometimes, however, the short height itself could be manifestation of major diseases caused by the problem of other internal organs. Accordingly, this study reports the cases of unexpected major diseases found in patients with short stature who have been observed in the outpation clinic and emphasizes the importance of early diagnosis of the diseases. Methods: The retrospective study has been done with the records of patients who were diagnosed as short sture with a basis of below 2 SD (Standard Deviation) or 3 percentile in the pediatric clinic of the Samsung seoul hospital from January of 1996 to July of 1997. The study excludes children patients who indicated vomiting, headache and other nervous diseases-related symptoms except short stature among brain tumor patients. Results : The results were as follows 1) Major internal organ problems have been found in 5 of 52 cases which were diagnosed as short stature after visiting the outpatient clinic with the chief complaint of growth retardation. 2) 2 of the 5 cases were diagnosed chronic renal failure and started dialysis treatment after several months. No specific problems were found in the urinalysis till the term of renal failure. 3) The problems on the Sella MRI test were found in 3 cases of 1 case craniopharyngioma, 1 case histiocytosis X and 1 case focal mass in the pituitary body. Conclusion: Although a few children have the problems in the major internal organs among patients who were diagnosed as short stature, major diseases were found in all 5 cases who have problem Accordingly, it is considered to be necessary to estimate other internal organs minutely.

웨슬리와 홀리클럽 운동에 관한 연구

염시동 한국 전문대학 기독교교육학회 1999 한국 전문대학 기독교교육학회 논문집 Vol.3 No.1

When John Wesley was appointed as a fellow at Lincoln College in November 1729, he began a meeting for trying to lead a pious life in Oxford campus with Charles, his younger brother, Morgan and Kirkhan. They used to get together three or four times a week in the evening and have a good time in reading the Holy Bible in Hellenic and Latin language. On Sunday evenings they read the books on theology, and on the other days They read the classics in Hellenic and Latin language. They were "the Biblelovers" who read the Bible very hard. That is to say, they were people of only one book-the Holy Bible. They became the Bible Christians who were comfortable to the interpretation of the first church about all living principles. Because of their this lives the Oxford students gave the members of the Holy club three mocking nicknames. The first nickname is "Methodist". It was given by Wesley's practical life of the thought that their discipline and pursuit for holiness and purity can be obtained only through obedience and training, and regular life. And later Wesley adopted the nickname as the name of the group. The second nickname is "enthusiast", which came our of the fact that their religious and social activities were very enthusiastic. The third nickname is "ceremononialist", which was given because of their active participation in the Communication service. On the other hand, the activities of the Holy club movement are as follows: The first is the study of the Sunday records for the purpose of theological understanding and insight by studying the Bible and classical spiritual books. The second is the pious life which can incorporate salvation and holy life, which depend on sincerity and good conduct in daily life. The third missionary work which practices the faith through love in social life. I think when the followings are strengthened an the basis of Holy club movement the right religious movement in Korean Christian church and college can be made. First, we have to try to find out the very core and message of the eternal gospel besides studying the Bible and practicing the spiritual discipline. which Wesley tried to learn from the tradition of the Firch church. Second, We must try to lead our daily lives for the glory of the Lord by having right interest in pious life and by trying to reach the wholeness of Christian-Wesley's main doctrine. Third, we should try to serve truly as workes of the Lord through the religious life only for us also for our neighbors the human relationship in which we can share love with them. On the Occasion of the 270th anniversary of the Oxford conversion, We will have to try to lead the Korean Methodist church and college to embody the basic ideologies and practical activities of Holy Club movement, and moreover we will have to provide a momentum for making today's corrupt society be born again.