The effect of Lfcin-B on non-small cell lung cancer H460 cells is mediated by inhibiting VEGF expression and inducing apoptosis

Shusheng Wang,Jiancheng Tu,Cuijie Zhou,Jianwei Li,Long Huang,Lei Tao,Lei Zhao 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.2

Lfcin-B, an antimicrobial peptide found invarious exocrine secretions of mammals, showed antitumoreffects. However, the effect and relative mechanism ofLfcin-B on non-small cell lung cancer is unclear. In thisstudy, assay of cell viability, quantitative real-time PCR,Western blot, annexin V/propidium iodide assay, flowcytometry and tumor-xenograft model were applied toelucidate the mechanism of Lfcin-B on non-small cell lungcancer NCI-H460 (H460) cells. Lfcin-B significantly suppressedthe proliferation of H460 cells in vitro. Additionally,the transcription and translation of the VEGF gene inH460 cells were restrained after exposure to Lfcin-B. Moreover, the apoptosis of H460 cells was induced byLfcin-B through stimulating caspase-3, caspase-9 andpreventing survivin expression on both the transcriptionand translation level. Meanwhile, Lfcin-B increased theproduction of reactive oxygen species and suppressed theRNA of antioxidant enzymes (GPX1, GPX2, SOD3 andcatalase) in H460 cells. Finally, Lfcin-B significantly preventedthe tumor growth in the H460-bearing mice model. These results indicated that Lfcin-B could be a potentialcandidate for the treatment of lung cancer.

Analysis for gear contact in synchronizer

Shusheng Feng(펑수성),Daesung Bae(배대성),Woonggi Kim(김웅기),Byoungchan Kim(김병찬) 한국소음진동공학회 2016 한국소음진동공학회 학술대회논문집 Vol.2016 No.4

Superior lubrication of dense/porous-coupled nanoscale C/WS₂ multilayer coating on ductile substrate

Xu, Shusheng,Liu, Yuzhen,Gao, Mingyu,Kang, Kyeong-Hee,Shin, Dong-Gap,Kim, Dae-Eun Elsevier 2019 APPLIED SURFACE SCIENCE - Vol.476 No.-

<P><B>Abstract</B></P> <P>Sputtered carbon material has been receiving much attention as a solid lubricant coating owing to its high hardness and low coefficient of friction. However, its relatively low wear resistance performance when applied on a ductile substrate has limited its use in certain applications. In this work, a porous WS<SUB>2</SUB> sublayer was introduced to the sputtered carbon-based nanoscale multilayer coating to improve its tribological properties. The microstructural, mechanical and tribological properties of the nanoscale C/WS<SUB>2</SUB> multilayer coating were systematically investigated using various analytical techniques. Through structural optimization, a durable coating with excellent wear resistance and low frictional performances could be attained for a ductile steel substrate. The excellent toughness allowed the coating to deform with the ductile substrate without fracturing during contact sliding process. Furthermore, the first self-destruction and then recombination behaviors of dense/porous-coupled nanoscale multilayer coating as well as the selective release of carbon component to the contact interface driven by the frictional interaction aided in maintaining low friction. As a result, the nanoscale multilayer coating showed approximately 100-fold greater wear resistance than that of pure hard carbon coating on a ductile steel substrate.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A hard/soft-coupled nanoscale C/WS<SUB>2</SUB> multilayer coating was constructed by sputtering. </LI> <LI> The multilayer coating exhibited excellent synchronized deformation with the ductile steel substrate. </LI> <LI> Multilayer coating on ductile steel showed 100-fold longer wear life than that of a stiff C coating. </LI> <LI> The rearranged coating on the formed deep wear track resulted in improved lubrication performance. </LI> <LI> That provided a novel way to develop lubricant protection layer on ductile material surface </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Selective release of less defective graphene during sliding of an incompletely reduced graphene oxide coating on steel

Xu, Shusheng,Liu, Yuzhen,Gao, Mingyu,Kang, Kyeong-Hee,Kim, Chang-Lae,Kim, Dae-Eun Elsevier 2018 Carbon Vol.134 No.-

<P><B>Abstract</B></P> <P>Recently, graphene has been gaining a lot of attention as a coating material that can be effectively utilized in reducing friction and wear of sliding components. In order to fully exploit the lubricious properties of graphene, the variation in the structure of the coating as sliding proceeds against a counter surface needs to be understood clearly. In this study, the structural evolution during sliding of an incompletely reduced graphene oxide (rGO) coating deposited on the 304 stainless steel by an electrodynamic spraying process was investigated. Experimental results showed that this coating possessed a low friction coefficient, below 0.05, under either low humidity air or dry N<SUB>2</SUB> gas condition. The micro-Raman and XPS analyses systematically revealed that less defective graphene structure was selectively released at the center region of the wear track. It was determined that the redistribution of graphene with different structures within the wear track was induced by frictional interaction at the sliding interface. Furthermore, it was determined that the degree of release of less defective graphene structure was proportional to both the applied normal force and sliding cycles.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Characteristic modes and flow structure of non-premixed flame in humid-air combustion

Zang Shusheng,Gu Xin,Ge Bing 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.2

and the flame stability limit in turbulent non-premixed flame. Two-dimensional Particle Image Velocimetry (PIV) meas-urements were made to quantify the velocity field, with and without steam injected. The results indicate the additionof steam decreases the recirculation flow and reduces the distance between the forward and aft stagnation points. Thedetailed stabilization regimes show that the critical fuel-to-air velocity ratios of the central fuel penetration in the humidair case are 16% to 22% lower, and the partially quenching limits are at least 25% lower. The decreased penetrationlimit is due to a reduction in momentum of the humid air. An analysis of flamelet concepts reveals that increased chem-

ZL-2, a cathelicidin-derived antimicrobial peptide, has a broad antimicrobial activity against gram-positive bacteria and gram-negative bacteria in vitro and in vivo

Jiancheng Tu,Shusheng Wang,Geping Wu,Yun Zuo,Lei Zhao 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

Alloferons are a group of naturally occurringpeptides primarily isolated from insects that are capable ofstimulating mouse and human NK cell cytotoxicity towardcancer cells. In this study, we found that a modified antibacterialpeptide had a broad range of action against bothgram-positive and gram-negative bacteria. A time-courseexperiment showed that CFU counts rapidly decreasedafter ZL-2 treatment, with the bacteria nearly eliminatedwithin 4 h. We also examined the synergy between thepeptide and antibiotics. The peptide ZL-2 resulted in asignificant synergistic improvement in the potencies ofampicillin, erythromycin and ceftazidime against methicillin-resistant bacteria. In addition, ZL-2 had no detectablecytotoxicity in mouse spleen cells or a mouse animalmodel. In the mouse model by i.p. inoculation with Escherichiacoli, timely treatment of i.p. injection with ZL-2resulted in 100-fold reduction in bacteria load in blood aswell as 80 % protection from death in the inoculated animals. In conclusion, we successfully identified a modifiedpeptide with maximal bactericidal activity. This study alsoprovides a potential therapeutic for the treatment of E. colisepticemia by increasing the activity of antimicrobials.

Investigating the Wear Mechanisms and Tribochemical Reactions of W-Based Coatings

Yuzhen Liu,Shusheng Xu 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

Removal of Cr(VI) from solution using UiO-66-NH2 prepared in a green way

Xiaoting Zhang,Shusheng Zhang,Gangfeng Ouyang,Runping Han 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7

A zirconium-based metal organic framework (UiO-66-NH2) was prepared using a greener approach forthe removal of hexavalent chromium (Cr(VI)) from solution. The characterization of UiO-66-NH2 was performedusing several methods, and the influence of the solution pH, adsorbent dosage, salt, adsorption time, Cr(VI) concentrationand adsorption temperature on adsorption quantity was performed in batch mode. UiO-66-NH2 can efficientlyremove Cr(VI) through the synergistic effect of adsorption and reduction. At pH solution of 1.1 and solid-to-liquidratio of 2 g·L1, the removal rate of 100mg·L1 Cr(VI) increased to 95.2%. The maximum adsorption capacity of UiO-66-NH2 from Langmuir model was 252mg·g1. The adsorption equilibrium process can be well described by Langmuir,Koble-Corrigan and Temkin models, while the kinetic process can be predicted by pseudo-second-order andElovich model. UiO-66-NH2 showed a good adsorption capacity in a wide range of pH (pH=1.1-10) and also had betterregeneration performance after five cycles using 0.01 mol·L1 NaOH; consequently, it can be a promising adsorbentfor the removal of Cr(VI) from solution.

Analysis of heat transfer performance of ORC direct contact heat exchanger by GRA-VMD-LSSVM model using optimization

Guanfeng Zheng,Qingtai Xiao,Shusheng Zhu,Hua Wang,Jian Geng,Shuang Zhao,Junwei Huang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7

The relationship between heat transfer effect and influencing factors in direct contact with evaporator isstudied. It is important to optimize the design and setting of direct contact heat exchangers and further improve theheat transfer process of heat exchangers by understanding the influencing factors of direct contact evaporator. Themethods used are grey correlation analysis, variational mode decomposition and least square support vector machinealgorithm to calculate the experimental heat transfer coefficient. The conclusions are that grey relation analysis can findthe complex relationship between different influencing factors and reduce the amount of data to improve the accuracyof prediction. Hence, the novelty of this paper is to propose a simple, efficient and accurate hybrid prediction model forVHTC prediction. The place where it goes beyond previous efforts in the literature is that different influencing factorson the heat transfer performance in the direct contact heat transfer process are considered. The results show that theprediction accuracy of the heat transfer coefficient can be improved by 7% by optimizing the data only with grey correlationanalysis. The prediction accuracy can be improved up to 53% after using the hybrid model.

Large-eddy simulation of low-swirl multi-nozzle combustion with co- and counter-swirling arrays

Weijie Liu,Bing Ge,Shusheng Zang,Huiru Wang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

Large-eddy simulation (LES) of CH 4 -air low-swirl flame was carried out in a multi-nozzle combustor with two burner configurations by using a premixed flamelet model. The multi-nozzle burner includes a co-swirling array where all five nozzles act in the same direction and a counter-swirling array where the center nozzle is in the opposite swirling direction to the outer nozzles. LES results are in good agreement with OH-planar laser-induced florescence data in terms of OH concentrations and combustion progress variables. Numerical results show that the flow of each nozzle is constant before merging. The neighboring flows interact with each other and generate a highvelocity zone with intensive turbulence. The kinetic energy in the interacting region for the co-swirling array is larger than that for the counter-swirling array. After neighboring flow combining, the flow develops into a unified swirling motion similar to a single swirling flow for the co-swirling array, whereas the flow maintains the individual swirling structures for the counter-swirling arrangement. However, the swirling array exerts minimal effect on multi-nozzle combustion in terms of the temperature distributions and combustion progress of premixed low-swirl multi-nozzle flames.