CGI-58 Protein Acts as a Positive Regulator of Triacylglycerol Accumulation in Phaeodactylum tricornutum

Shu Qin,Pan Yufang,Hu Hanhua 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.2

Comparative gene identification-58 (CGI-58) is an activating protein of triacylglycerol (TAG) lipase. It has a variety of catalytic activities whereby it may play different roles in diverse organisms. In this study, a homolog of CGI-58 in Phaeodactylum tricornutum (PtCGI-58) was identified. PtCGI-58 was localized in mitochondria by GFP fusion protein analysis, which is different from the reported subcellular localization of CGI-58 in animals and plants. Respectively, PtCGI-58 overexpression resulted in increased neutral lipid content and TAG accumulation by 42-46% and 21-32%. Likewise, it also increased the relative content of eicosapentaenoic acid (EPA), and in particular, the EPA content in TAGs almost doubled. Transcript levels of genes involved in de novo fatty acid synthesis and mitochondrial β-oxidation were significantly upregulated in PtCGI-58 overexpression strains compared with wild-type cells. Our findings suggest that PtCGI-58 may mediate the breakdown of lipids in mitochondria and the recycling of acyl chains derived from mitochondrial β-oxidation into TAG biosynthesis. Moreover, this study potentially illuminates new functions for CGI-58 in lipid homeostasis and provides a strategy to enrich EPA in algal TAGs.

Novel miR-1958 Promotes Mycobacterium tuberculosis Survival in RAW264.7 Cells by Inhibiting Autophagy Via Atg5

( Shuqin Ding ),( Yuliang Qu ),( Shaoqi Yang ),( Ya’e Zhao ),( Guangxian Xu ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.6

Autophagy is crucial for immune defense against Mycobacterium tuberculosis (Mtb) infection. Mtb can evade host immune attack and survival within macrophages by manipulating the autophagic process. MicroRNAs (miRNAs) are small, non-coding RNAs that are involved in regulating vital genes during Mtb infection. The precise role of miRNAs in autophagy with the exits of Mtb remains largely unknown. In this study, we found miR-1958, a new miRNA that could regulate autophagy by interacting with 3'UTR of autophagy-related gene 5 (Atg5). In addition, Mtb infection triggered miR-1958 expression in RAW264.7 cells. What's more, miR- 1958 overexpression blocked autophagic flux by impairing the fusion of autophagosomes and lysosomes. Overexpression of miR-1958 reduced Atg5 expression and LC3 puncta while inhibition of miR-1958 brought an increase of Atg5 and LC3 puncta; the opposite results were observed in detection of p62. The survival of Mtb in RAW264.7 cells transfected with mimic of miR-1958 was enhanced. Taken together, our research demonstrated that a novel miR-1958 could inhibit autophagy by interacting with Atg5 and favored intracellular Mtb survival in RAW264.7 cells.

Research on the Load-slip Properties of Corrugated rib connectors' Push-out Test

Shuqin Li,Lichao Su,Zhibin Sun 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.4

The failure characteristic and load-slip performance of corrugated rib connectors under static load were investigated usingthe push-out test combined with Finite Element Analysis (FEA). The results obtained from experiment and FEA indicate thatthe shear bearing capacity of the corrugated rib connectors are closely relate with the key factors including cubic strength ofconcrete (fcu), the open area of the steel plate (Ac1), perforated rebar tensile yield strength (fy) and Cross-sectional area (Ay),horizontal projection area of corrugated rib (= dwhw). In elastic stage, the overall shear stiffness of the corrugated rib connectorare mainly affected by the shear stiffness of the concrete bars. And in elastic-plastic stage, the shear stiffness of the concretebars and perforated rebar pronouncedly influence the overall shear stiffness of the corrugated rib connector. Finally, a linearmodel and a power law model for the load-slip behavior at elastic stage and elastic-plastic stage are provided based on theregression statistical analysis, respectively.

Bounded matrices over regular rings

Shuqin Wang,Huanyin Chen 대한수학회 2006 대한수학회보 Vol.43 No.1

In this paper, we investigate bounded matrices over regular rings. We observe that every bounded matrix over a regular ring can be described by idempotent matrices and invertible matrices. Let $A,B\in M_n(R)$ be bounded matrices over a regular ring $R$. We prove that $(AB)^d=U(BA)^dU^{-1}$ for some $U\in GL_n(R)$.

A NEW "MIDDLE" CRETACEOUS ZALAMBDALESTID MAMMAL, FROM A NEW LOCALITY IN JILIN PROVINCE, NORTHEASTERN CHINA

Shuqin Zan,Craig B. Wood,Guillermo W. Rougier,Liyong Jin,Jun Chen,Charles R. Schaff 한국고생물학회 2006 고생물학회지 Vol.22 No.1

중국 길림성의 Gongzhuling 시 근처의 새로운 백악기 화석지에서 산출된 화석은 두개의 불완전한 포유류 하악골을 포함하는데 이 표본은 태반류 Zalambdalestidae과에 속하는 새로운 속과 종이다. 화석지는 Songliao분지의 넓게 분포하는 Quantou층의 가창자리에 위치하는데 Songliao분지의 시대는 Aptian~Cenomanian으로 알려져 있다. 그러나 Quantou층이 Cenomanian 보다 더 젊을 것 같지는 않다. 두 하악골은 크고 납작한 첫 번째 앞니를 갖고 있는데 Kulbeckia kulbecke같은 종과 비교해 보면 원시적이고 진화적인 특징들이 흥미롭게 섞여 있다. 예를 들면, Gongzhuling 표본은 5개의 앞어금니 (거의 완전한 어금니 형태의 앞어금니)와 단지 3개의 앞니, 그리고 한 개의 낮고 단 뿌리의 송곳니를 가진다. 5번째 앞어금니와 첫 번째 어금니의 trigonids는 상대적으로 눌리지 않고 열려있는 형태다. 이 화석지는 “중기” 백악기 척추화석들에 대한 새로운 정보를 확보할 중요한 장소로 알려지게 될 것이며 가까운 미래에 이곳에서 새로운 포유류, 공룡, 그리고 다른 표본들이 기재될 것이다. Fossil specimens from a new Cretaceous locality near Gongzhuling City in Jilin Province, China, include two incomplete mammalian dentaries which represent a new genus and species referable to the eutherian family Zalambdalestidae. The locality is in basin-margin outcrops of the Quantou Formation, which is widely spread in the subsurface of Songliao Basin and which has been assigned ages ranging from the Aptian to the Cenomanian stages of the Cretaceous; it seems unlikely that the Quantou Formation could be younger than Cenomanian. Both dentaries possess an enlarged, procumbent first incisor combined with an interesting mosaic of both plesiomorphic and derived dental characters compared to taxa such as Kulbeckia kulbecke. For example, the new Gongzhuling specimens appear to show five premolars (including an almost fully molariform ultimate premolar) combined with only three incisors and a low but single-rooted canine. The trigonids on p5 and m1 are relatively open and non-compressed. This locality is likely to emerge as an important source of new information on "middle" Cretaceous vertebrates as additional mammalian, dinosaurian, and other specimens are described from it in the near future.

The relationship between the variants in the 5'-untranslated regions of equine chorionic gonadotropin genes and serum equine chorionic gonadotropin levels

ShuQin Liu,Song Lian,YunZhou Yang,ChunZheng Fu,HongYing Ma,ZhiYao Xiong,Yao Ling,ChunJiang Zhao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12

Objective: An experiment was conducted to study the association between the single nucleotide polymorphisms (SNPs) in 5'-untranslated regions (5'-UTR) of equine chorionic gonadotropin (eCG) genes and the serum eCG levels. Methods: SNPs in 5'-UTR of eCG genes were screened across 10 horse breeds, including 7 Chinese indigenous breeds and 3 imported breeds using iPLEX chemistry, and the association between the serum eCG levels of 174 pregnant Da’an mares and their serum eCG levels (determined with ELISA) was analyzed. Results: Four SNPs were identified in the 5'-UTR of the eCGα gene, and one of them was unique in the indigenous breeds. There were 2 SNPs detected at the 5’ end of the eCGβ subunit gene, and one of them was only found in the Chinese breeds. The SNP g.39948246T>C at the 5'-UTR of eCGα was associated significantly with eCG levels of 75-day pregnant mare serum (p<0.05) in Da’an mares. Prediction analysis on binding sites of transcription factors showed that the g.39948246T>C mutation causes appearance of the specific binding site of hepatocyte nuclear factor 3 forkhead homolog 2 (HFH-2), which is a transcriptional repressor belonging to the forkhead protein family of transcription factors. Conclusion: The SNP g.39948246T>C at the 5'-UTR of eCGα is associated with eCG levels of 75-day pregnant mare serum (p<0.05).

Tunable Shape Memory Polyurethane Networks Cross-Linked by 1,3,5,7-Tetrahydroxyadamantane

Shuqin Fu,Jiaping Zhu,Shaojun Chen 한국고분자학회 2018 Macromolecular Research Vol.26 No.11

As a new type of smart polymers, the shape memory polyurethanes attract increasing attention because of their wide use in various applications. Herein, a serials of novel thermo-responsive shape memory polyurethane networks were developed by chemical cross-linking of polycaprolactone with 1,3,5,7-tetrahydroxyadamantane. The polyurethane networks were characterized in relation to their microstructure, cross-linking density, surface appearance, thermal properties, static and dynamic mechanical properties, and shape memory behavior. It was found that the prepared polyurethane networks can exhibit excellent thermo-responsive shape memory properties at body temperature by controlling the molar ratio of 1,3,5,7- tetrahydroxyadamantane and polycaprolactone. Moreover, the incorporation of 1,3,5,7-tetrahydroxyadamantane substantially improved the mechanical properties of the final polyurethane networks. The versatility of the synthesized polyurethane networks is reflected by its dual- and triple-shape memory performance. It can be anticipated that the synthesized polyurethane networks could be used as smart materials for applications involving shape memory polymers.

Robust Finite-time Control for Neutral Systems with Time-varying Delays via Sliding Mode Observer

Shuqin Wang,Baoping Jiang,Cunchen Gao,Yonggui Kao 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.5

This paper is concerned with the problem of robust finite-time sliding mode control (SMC) for a classof continuous-time uncertain neutral systems with time-varying delays. Firstly, based on the constructed slidingmode observer, an integral sliding surface is put forward on the estimation space. Secondly, the synthesized SMClaw guarantees the finite-time reachability of the predefined sliding surface. Thirdly, through finite-time stabilityanalysis, sufficient conditions are established to guarantee the required performance of the system during bothreaching phase and sliding motion phase. Finally, a practical example is provided to verify the effectiveness of theproposed method.

The Economic and non-Economic Analysis of U.S.-China Trade Deficits

MA ShuQin 한국통상정보학회 2004 통상정보연구 Vol.6 No.3

While the bilateral trade volume between China and U.S. has been growing rapidly, the trade deficits of U.S. to China has also been expanding. This growing trade deficit of U.S. to China has several reasons: the increase of foreign direct investment to China, the transfer of trade deficit origins, the intervention of U.S. domestic politics to China-U.S. trade relations, and U.S. direct control on export to China. However, the increased U.S. trade deficit to China does not mean that U.S. is in a disadvantaged position in its economic relations with China, or its international competitiveness is deteriorating. When U.S. surplus in service trade to China is included, the picture would be very different. Also, as internationalization progresses and China’s industrial structure adjusts, the trade deficit of U.S. to China would narrow.

BOUNDED MATRICES OVER REGULAR RINGS

Wang Shuqin,Chen Huanyin Korean Mathematical Society 2006 대한수학회보 Vol.43 No.1

In this paper, we investigate bounded matrices over regular rings. We observe that every bounded matrix over a regular ring can be described by idempotent matrices and invertible matrices. Let A, $B{in}M_n(R)$ be bounded matrices over a regular ring R. We prove that $(AB)^d = U(BA)^dU^{-1}$ for some $U{\in}GL_n(R)$.