Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

시각화 초심자들을 위한 시각화 지침 조사

이세희(Sehi L’Yi),신동화(DongHwa Shin),장유리(Youli Chang),이재용(Jaeyong Lee),서진욱(Jinwook Seo) 한국정보과학회 2018 한국정보과학회 학술발표논문집 Vol.2018 No.6

화면 훔쳐보기 문제에 대한 보다 넓은 이해를 위한 연구

이세희(Sehi L’Yi),조재민(Jaemin Jo),신동화(DongHwa Shin),서진욱(Jinwook Seo) 한국HCI학회 2018 한국HCI학회 학술대회 Vol.2018 No.1

화면 훔쳐보기 문제(shoulder-surfing problem)를 다룰 때, 패스워드나 스마트폰 잠금 해제 패턴 등 중요한 개인정보를 보호하기 위해서는 다양한 연구가 이루어진 반면 사적인 문자 보내기 같은 일상에서 중요한 정보들을 보호하려는 연구는 드물다. 이 논문에서는 일상생활에서 발생하는 화면 훔쳐보기 문제를 보다 넓게 이해하기 위해 대규모의 온라인 설문(n = 350)을 수행하고 그 결과를 발표한다. 설문결과에 의하면 사람들은 사용자 인증 과정 뿐만 아니라 문자 작성, 사진 탐색, 영상 재생 등의 가벼운 상황에서도 훔쳐보기 문제를 경험한다는 것을 알 수 있었다.

대학생의 수면양상과 주간졸음증에 영향을 미치는 요인

이혜련(Hyeryeon Yi),신미경(Mee-Kyung Shin),원종순(Jongsoon Won) 기본간호학회 2012 기본간호학회지 Vol.19 No.4

Purpose: The purpose of this study was to investigate sleep patterns and predictors of excessive daytime sleepiness (EDS) in university students. Methods: Participants were 120 university students who were attending two universities in S-city and C-city. Data were collected from May 20 to June 15, 2012 using self-report questionnaires which included Johns’Epworth Sleepiness Scale, Yi’ Sleep Quality Scale, and Beck Depression Inventory. Data were digitalized and analyzed using frequency, percentages, means and standard deviations, Wilcoxon rank sum test, Chi-square test, Fisher’ exact test, and multiple logistic regression with SAS 9.0. Results: Mean total sleep time was 6.6 hours on weekdays, 8.1 hours on weekends. Mean sleep latency was 19.1 minutes and the score for mean sleep quality was 22.6. Prevalence of EDS was 12.5%. Depression was significantly different between EDS and Non-EDS students (t=2.17, p=.030). Multiple logistic regression showed that the only factor associated with EDS was depression (adjusted odds ratio of depression=5.33, 95% Confidence Interval=1.49-19.04). Conclusion: Results of this study indicate that university students experience short sleep time, low sleep quality, and common EDS with depression, suggesting that students with complaints of EDS should be completely assessed for depression as well as sleep problems.

다면적 인성검사(MMPI) 군집 분석을 통한 대학생 내담자의 유형 분류

이민영,강종구,홍창희,이신화 부산대학교 학생생활연구소 2000 硏究報 Vol.32 No.2

본 연구에서는 학생생활연구소를 방문한 대학생 156명을 대상으로 다면적 인성검사(MMPI) 프로파일을 군집 분석하고 유형별 특징을 살펴보았다. MMPI 만용에 따라 4개의 군집으로 분류할 수 있었고, 집단 1(31%)은 방어적 집단, 집단 2(8%)는 도움 호소 집단, 집단 3(50%)은 정상 프로파일 집단, 집단 4(12%)는 신경증적 집단으로 정의되었다. 4개 집단 중에서 집단 2(도움 호소 집단)와 집단 어신경증적 집단)는 일상 생활에서 불편함을 경험할 가능성이 많은 집단이므로 이 두 집단에 대한 주의깊은 관심이 필요할 것으로 여겨진다. 집단2(도움 호소 집단)는 전반적인 적용에서 어려움이 많을 것으로 예상되는 집단으로 사회적인 접촉을 회피하고 긴장, 불안, 우울을 경험하고 있는 것으로 나타났고, 집단 익신경증적 집단)에서는 신체화 및 충동성이 문제가 될 수 있을 것으로 예상된다. 각 집단에 대해 고려해야 할 사항들 및 연구의 시사점, 제한점을 논의하였다. The purpose of this study was to classify subtypes of the Minnesota Mllltiphasic Persomanality Inventoy(MMPI) profiles far university student clients. The MMPI 13 scales(L,F,K and 10 clinical scale) from 156 university students seeking psychological services from a university persfmality assessment service were investigated using cluster analysis. Four group were identified. The 48 of 156 participants(31%) were classified as group 1 and were concerned about disclosing informatifm that might be socially undesirable. When they perceived that test feedback challenges their self-concepts, this group typically rejected or denied the importance of suck feedback. The 12 of participants(8%) were classifled as group 2. This group admitted to some problems and afeared to be in enough distres to be motivates for therapy, but was not lithely to trust therapist easily. F elevation of this group migllt indicate fragility and weak ego strength. The half of participants(50%) was classified as group 3 and didn't show elevated scare in any scale. This group was named 'normal-reactilnl group'. The 18 of participants(12%) were classified as group 4 and were of ten lacked psychological might. Group 4 might become quite defensive when their poblems attributed to psychological causes, reacting with denial and hostility. This group might ask in indirect ways that people see as'manipulative'. The implications and limitatifms of this study were discussed.

유기박막 광도파로형 칼슘이온센서의 개발

이수미,임남주,김창원,강신원 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

New active organic thin film optical wavegudie ion sensor is proposed. Poly-dimethylsiloxane(RTV3140) membrane based on indicator tetramethylmurexide (TMX) and co-polymer matric membrane based chromoionophore(ETH5294) were used as active waveguide itself. Utilizing thin film membrane, fast respense time and high sensitivity are obtained. And sensor could be measured a wide range of Ca^(++) concentrations ranging from 10^(-6) to 1M by measuring the absorbance change at the 488nm and 514nm wavelength of light. The dependence of attenuation of guided wave on the properties of transduction layer is discussed.

크레아틴 로딩이 지구운동 및 스프린트 파워발휘에 미치는 효과

오이표,김희수,이신언 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

A study was conducted on the effect f creatine loading on persistent exercise and outcome in sprint which was performed immediately after such exercise and subjects of this study were 12 cycle load athletes. Persistent exercise for the purpose of experiment was carried out for 2 hours and 30 minutes. Then it was continued until the condition of tiredness and exhaustion was reaching with showing density of lactate in blood of 4mmol/ℓand then immediately thereafter for 10 seconds 5 sets maximum sprint were carried out. The subject athletes carried out C Performance which featured absorption of placebo liquid during the exercise involving creatine loading prior to experiment exercise, CC Performance featuring absorption of creatine liquid during the exercise involving creatine loading and P Performance featuring absorption of placebo liquid during the exercise without creatine loading-thus totally 3 Performances were carried out. Comparing with P Performance C Performance showed significant improvement in all the 5 sets in terms of peak power and average power for maximum sprint. On the other hand in CC Performance it was same as P Performance. Creatine loading and creatine absorption liquid did not have any effect on duration of persisting exercise until reaching to fatigue and exhaustion. From the result of this study it could be observed that creatine loading improved maximum sprint ability within a short period of time after persistent exercise leading to fatigue and exhaustion However such effect declined when creatine liquid was absorbed during the exercise.

강남콩 및 흑대두에서의 Phytohemagglutinin 抽出

姜信珠,李喜子 慶北大學校 師範大學 1984 敎育硏究誌 Vol.26 No.-

This experiment was conducted to study on the phytohemagglutinins which were extracted from black soybeans and kidney beans, their hemagglutinating activity, the purity, and the relative contents of protein though cellulose acetate electrophoresis. The results were summarized as following: The proteins of phytohemagglutinins from black soybeans were consisted of 32.2% of albumin, 13.3% of α_1-globulin, 11.1% of α_2-globulin, 30.3% of β-globulin, and 13.4% of γ-globulin. The proteins of phytohemagglutinins from kindney beans were consisted of 23.0% of α_2-globulin and 77.0% of γ-globulin. The hemagglutinating activities of black soybeans and kidney beans were 1,311 HU/㎎. protein and 2,438HU/㎎·protein respectively.

노인의 임종과정에 대한 태도 유형 분석

김순이,김신미 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: This study was performed to identify and understand the nature of attitude patterns regarding dying process exhibited in the elderly persons who lived in the community setting. Method: Q methodology was applied. Ten elderly were selected from the senior centers and requested to answer open ended questions by using written questionnaires. Additionally, 12 other elderly from different senior centers were interviewed in depth, and their statements were recorded via voice pen by researchers to derive Q-population. From the 120 Q-population, 33 Q-sample were selected, 22 P-sample were rated by 1 to 9 oint scale respectively for Q-sorting. For Q-type analysis, pc QUNAL was used. Result: Three types of attitude patterns were identified, which were valuing dignity, seeking provision of nature, and avoiding of dying process. Conclusion: The significance of this study is as follows : Discovery of 3 attitude patterns of elderly toward dying. better understanding about elderly's perspectives for favorable dying, and informations for possible development of nursing strategies for elderly who is facing death.

지구운동 후의 식사조성이 근중성지방과 지구운동의 성과에 미치는 효과

이신언,오이표,김한수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

This study was carried out for the purpose of examining the effect of eating on muscular neutral fat and outcome of persistent exercise. Seven men who were taking persistent training had 2 hours of cyclying exercise at an intensity of 65%VO₂max, Thereafter the examinees took meals containing high level of sugars and fats and then carried out 1600kJ by self pace after fasting 12 hours. In the Performance taken 2 hours before starting cycling training (33.0+/- 2.3 vs. 37.0 +/- 2.1mmol/㎏ dry weight ) and in the Performance taken thereafter (30.9+/- 2.4 vs. 32.8+/- 1.6 mmol/㎏ weight) there was no difference in muscular neutral fat. However eating more fatty food showed higher value of muscular neutral fat prior to 2nd self paced exercise in comparison with taking more sugary food. (44.7+/- 2.4 vs. 27.5+/- 2.1 mmol/㎏ dry weight). In terms of outcome of self-paced cycling training Performance where highly fatty food was taken showed significant decline, This outcome did not show any significant change in muscular neutral fat before and after cycling training of medium level intensity but it was observed that absorption of highly fatty food heightened accumulation of muscular neutral fat rather than taking highly sugary food.