Neuropeptide γ의 구조 및 생리활성

구희정,서정길,김은희,허민도,정준기,박장수,강신원,박남규 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

생리활성을 지닌 신경펩타이드의 구조와 활성간의 관계를 규명하기 위하여 고상법으로 합성한 세종류의 neuropeptide γ(mammalian-,trout- 그리고 goldfish-neuropeptide γ)를 사용하여 연구하였다. Circular dichroism spectra에 의하면 mammalian-,trout- 와 goldfish-neuropeptide γ는 완충액 조건하에 모두 random한 구조를 나타내었다. 중성 및 산성 지질 존재 하에서, mammalian-과 trout- neuropeptide γ는 여전히 random한 구조를 취하였다. 그러나, goldfish-neuropeptide γ는 중성 및 산성지질하에서 부분적으로 α-helix구조를 나타내었다. 장관 수축활성에 있어서는 carp 장관, guinea-pig 회장 그리고 rat십이지장에 대하여 비교하였다. Carp에 대해서는 goldfish-neuropeptide γ= trout- neuropeptide γ>mammalian-neuropeptide γ순으로 활서이 나타났다. 그러나, guinea-pig 회장과 rat십이지장에 대해서 mammalian-neuropeptide γ는 어류 유래성 neuropeptide g들 보다 높은 수축활성을 나타내었다. 이러한 결과들은 neuropeptide γ들이 종-특이적인 활성을 나타낸다는 것을 제시한다. The relationship between structure and biological activity was studied on the three neuropeptides(mammalian-,trout- and goldfish-neuropeptide γ)that were syntheized by the solid-phase method. Circular dichroism spectra showed that mammalian-,trout- and goldfish-neuropeptide γadopted an undered structure in buffer solution. In the presence od neutral and acidic liposomes, mammalian-and trout- neuropeptide γ also took a random structure. However, goldfish-neuropeptide γ took an α-helical structure in acidic liposomes. The intestinal motility response was investigated with carp interstines, guinea-pig ileums and rat duodenums. In case of carp intestine, contractine activity was as follows:goldfish-neuropeptide γ= trout- neuropeptide γ> mammalian-neuropeptide γ. On the other hand, the contracile activity of mammalian-neuropeptide γ was more potent than trout- and goldfish-neuropeptide γ in the guinea-pig ileums and rat duodenums. these results suggest that neuropeptide γs showed the species-specific activity.

치과의원과 대학치과병원 교정환자의 통제소재 특성

구승준,이신재,김태우,장영일 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.6

환자가 의료기관을 선택할 때 작용하는 요인은 매우 다양하며 이에 대한 많은 연구들이 있었으나 대부분 외부적인 요인에 대한 연구가 많았고 의료기관 선택 시 환자의 정신-심리학적인 면에 대한 정보는 부족한 실정이다. 이에 본 연구에서는 비교적 선택진료(elective treatment)의 측면이 많은 교정치료를 받기 위해 치과의원과 대학치과병원에 내원한 환자들이 의료기관을 선택하는 여러 요인들 중 정신-심리학적 요인을 알아보기 위해 인간의 행동을 예측하는데 유용하다고 알려진 통제소재(Locus of control)검사를 이용하였다. 교정치료를 목적으로 서울대학교 치과병원에 내원한 환자 934명 및 치과의원에 내원한 환자 1466명을 대상으로 내외통제소재검사를 시행한 결과 대학치과병원 교정환자들이 치과의원 교정환자에 비하여 외적통제 경향이 높았으며 이러한 결과에 비추어 대학치과병원 교정환자들이 치과의원 교정환자들에 비해 의료진의 권위에 의존하는 정신-심리학적 경향이 존재하는 것으로 나타났다. Objective: Mary factors are considered when a patient chooses the type of dental institution they wish to receive treatment from. Numerous studies have been conducted regarding this issue. However, more research is needed in analyzing the patient's psychological aspects when he/she decides to choose the dental institution they wish to receive treatment from. Methods: A locus of control test was utilized to analyze the psychologica1 aspects in orthodontic patients of a university denta1 hospital and a private dental clinic. The locus of control test is known to be useful in predicting a human being's behavior Both intrinsic and extrinsic locus of control tests were carried out in 934 orthodontic patients attending Seoul National University Dental Hospital and 1466 orthodontic patients from a private dental clinic. Results: The orthodontic patients at the university dental hospital showed a stronger extrinsic locus of control compared with those from the private dental clinic. Conclusion: The orthodontic patients at the university dental hospital value the doctor as the authority figure and the treatment quality more than those at the private dental clinic.

Propranolol·HCl-ion-exchange Albumin Microcapsules의 방출 특성에 관한 연구 : an in Vitro Propranolol Release Evaluation

박신아,김길수,구영순 梨花女子大學校 藥學硏究所 1992 藥學硏究論文集 Vol.- No.2

Empty cross-linked albumin microcapsules were prepared by chemical denaturation method using glutaraldehyde(GA) or terephthaloyl chloride(TP) as a cross-linking agent. The binding efficiency of cross-linked albumin microcapsules was evaluated as a function of various conditions. Increasing of the concentrations of croos-linking agent, stirring time, the amount of microcapsules and the initial Propranolol HCI(PPH, HCI) concentration reduced the binding ability of these microcapsules. But the increasing of the mean particle diameter increase that of these microcapsules. The release rates of PPH, HCI from the ion-exchange albumin microcapsules were retarded compared with the powder. And the total amount of drug released was dependent on the nature, the concentration of ionic strength of the cations used.

中學生의 環境差에 따른 醫療惠澤에 關한 調査硏究 : Focusing on chung-Nam

姜信一,具一會 公州大學校스포츠科學硏究所 1988 스포츠科學硏究所論文集 Vol.- No.2

This study is measure the gap of the surroundings of the medical benefaction and check the problems of it and a reform measure with analysis of the medical benefaction condition of the middle school students in large and small cities and farming and fishing areas. The middle students' problem of the hygiene education and the importance of it was discovered through this study. This study is based upon the data which were answered by the second grade students at middle school in Chung-nam povince. Ⅰ. The findings are as follows; 1. Concentration of the medical facilities and institutions in the cities causes the absence of them in the farming and fishing areas and the student in the fishing and farming areas to have the serious problems that they can not manage and maintain their health. 2. Because the individual student is negligent of the management of his hygiene, he has been attacked with many diseases without relation to the gap of his surroundings. 3. In farming and fishing villages, they are neglected to be treated at the hospital. They mainly depend on folk medicines and household medicines. 4. Only a few students use their school protective institution in spite of shortage of medical facilities in farming and fishing areas. 5. As people in the country as well as in the cities don't join the medical security system in large numbers, they are susceptible to infectious diseases. This runs counter to the improvement of national health. 6. In farming and fishing areas, many students are indifferent to the matter of preservation of health. And they are also in lack of medical facilities. 7. There is all the difference in the benefits of medical treatments be teween town and country. Ⅱ. Proposition Based upon the findings of the study, the following is suggested; 1. As one problem of this study, whether the condition of the health and medical benefaction in the farming and fishing areas influence on the society is questioned. 2. It is necessary to endow them with the time for the hygiene education and to educate them the realistic contents. 3. In order to improve the level of people's healty consciousness it is desirable for the public information of health related to the students and their parents to be preceded and to expand urgently the execution of the medical insurance. 4. Considering the regional speciality, of the lack of the medical facilities and institutions in the farming and fishing area protective rooms are to be used.

Propranolol·HCI-ion-exchange Albumin Microcapsules의 방출 특성에 관한 연구 : an in Vitro Propranolol Release Evaluation

박신아,김길수,구영순 梨花女子大學校 韓國生活科學硏究院 1992 韓國生活科學硏究院 論叢 Vol.49 No.-

Empty cross-linked albumin microcapsules were prepared by chemical denaturation method using glutaraldehyde(GA) or terephthaloyl chloride(TP) as a cross-linking agent. The binding efficiency of cross-linked albumin microcapsules was evaluated as a function of various conditions. Increasing of the concentrations of cross-linking agent, stirring time, the amount of microcapsules and the initial PropranololㆍHCl(PPHㆍHCl) concentration reduced the binding ability of these microcapsules. But the increasing of the mean particle diameter increase that of these microcapsules. The release rates of PPHㆍHCl from the ion-exchange albumin microcapsules were retarded compared with the powder. And the total amount of drug released was dependent on the nature, the concentration of ionic strength of the cations used.

전략적 환경적응력과 조직내부적 적합성이 중소기업의 성과에 미치는 영향

권구혁,신진교 한국중소기업학회 2003 中小企業硏究 Vol.25 No.2

본 논문은 중소기업의 경쟁전략, 기업환경 및 조직구조간의 관계 및 이들 관계의 적합성이 중소기업의 성과에 어떠한 영향을 끼치는가를 분석하고 있다. 179개 중소기업으로부터 설문자료를 수집·분석한 경과 중소기업의 경우에도 적합성 이론이 적용된다는 점을 알 수 있었다. 본 논문에서 제시한 가설들은 가설 2와 5를 제외하고는 부분적 혹은 전적으로 지지되었다. 전체적으로 볼 때, 가설에서의 예측은 성과가 낮은 기업보다는 성과가 높은 기업에서 보다 강하게 나타나고 있으며 부분적으로 지지된 가설들도 주로 성과가 낮은 기업의 영향을 받았다는 사실을 알 수 있었다. 성과 수준별로 표본을 구분하여 경쟁전략과 조직구조 및 환경불확실성의 관계를 살펴 본 결과, 차별화전략을 추구하는 기업이 높은 성과를 달성하기 위해서는 환경과 전략의 적합성이 중요한 반면, 저원가전략의 경우에는 전략과 조직구조와의 적합성이 높은 성과를 얻는데 중요하다는 점을 알 있었다. 이는 결국, 환경-전략-구조간의 적합성은 전략의 유형에 따라 성과에 관련된 함의가 다를 수 있으며 또한 전략 유형에 따라 중요한 적합성도 다를 수 있다는 점을 시사하고 있다. 마지막으로 본 연구결과에 대한 해석과 토론, 이론적 및 실무적인 시사점과 한계점, 그리고 향후의 연구방향이 제시되고 있다. This study examines the relationships among environment, strategy, and structure and the impact of fit among these three constructs on small and medium sized firm's performance. The data were collected from 179 small and medium sized firms located in Daegu and Kyungbuk district area. Empirical results showed that fit among environment, strategy, and structure is still important for small and medium sized firms. Firms with high fit were performing significantly better than firms with low fit. An important finding of this research is that fit among environment, strategy, and structure is not universally applicable for all types of strategy. High performers with externally oriented strategy (i.e., differentiation strategy) achieved external fit, while high performers with internally oriented strategy (i.e., cost leadership) pursued internal fit. It is also found that most of hypotheses are better supported for high performing firms than for low performing firms. The implications of these findings, limitations, and future research directions were also discussed.

大學綜合試驗 出題에 關한 硏究

卞烘圭,吳鎭坤,申鎔鎭,金泳喆,鄭求福,柳哲鍾,洪允杓 全北大學校 1977 論文集 Vol.19 No.-

The Comprehensive Examination for the undergraduate students is planned to adopt the Jeonbug National University for the purpose of enhancing the quality of its educational outputs. In order to effectively measure and evaluate the level of student progress toward the minimun standard as the students who have finished their general education and basic education for their major field study, the university has decided to make an item pool related to the subjects for the examination. This study is an attempt to make good items of five required courses among general education subjects which are Korean Language, English, Korean History, National Ethics. and Natural Science and are expected to be the subjects of the examination. Instead of making those items separately from the regular examination during the academic semesters, the university plans to collect and use good items from the usual mid-term and final examinations of those subjects. A key concern, in this study, therefore, is to analyze all the items used for the mid-term and final examinations of those subjects during the regular academic semesters. From the data of an about 100 sample in each subject at each time of total three tests, the difficulty index, discrimination index, the response rate of students on each item, and other general descriptive statistics are calculated and then good items are pooled for the future use for the Comprehensive Examination. Anhother important concern in this study is this study is given to the collection and analysis of controversial issues and problems on the process of operating the examination at other universities where the Comprehensive Examination is already adopted. The data from four institutions and from opinions of six participants of this study are used. The following main findidings are summarized : 1. The items have generally shown to be easy while the degree of discrimination is low. 2. Among the five subjects, Korean Language shows the highest rate of right answer (difficulty index) while English the strongest one in the discrimination index. 3. When comparing two types of test between the multiple choice and the short-answer, the latter seems to be powerful in both difficulty and discriminative. 4. The only one question of essay type test reveals clear limitations in scoring and evaluating students progress. 5. All items are constructed under the consideration of content-referenced base, not of the basis on behavioral aspect. 6. The analysis of issues and problems related to the Comprehensive Examination suggests that it is acceptable to test in terms of required subjects of general education and a few most basic subjects of major areas of study. 7. The examination can be functionally operated when it is held at the end of the first year of and before the beginning of the second year of the college years. The length of the examination is usally held from three to five hours. 8. Since the examination should be existed on the idea of criterion-referenced base rather than of norm-referenced one because of its testing the qualification of the entering behavior of students for the further study in their major field, the standard of the pass-and-failure should be set on at least 60 percent of correct answers in each subject. But there must be provided at least two additional opportunities for the make-up examination for those who failed in the first one. 9. A special committee must be established in order to deal with all the details related to the Comprehensive Examination.

MPLS망에서 RSVP와 DiffServ연동모델의 성능분석

최승권,오구영,지홍일,신승수,조용환 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.1

The explosive growth of the internet traffic has imposed tremendous stress on routers, particularly, in the core network. Today's IP services reflect the unpredictable and undifferentiated packet loss and jitter characteristics of traditional best-effort routers. For above requirements, it is required not only the high-speed transmission of the packet, but the new routing function, the guarantees of QoS and bandwidth, the supports of user-requested services MPLS is proposed by the IETF and many other research institute as the solutions for this problem. In this paper, analyze performance of QoS Model applied RSVP over DiffServ to MPLS network. this model is each RSVP applied access network and DiffServ applied core network. In Result, It is amiss that basis MPLS net uses to core network because the average packet loss rate and average delay time are increased if traffic increases. Experiment result was seldom difference in case of network that apply each RSVP, DiffServ, RSVP over DiffServ. RSVP over DiffServs case packet loss rate and delay time were low than DiffServ and the packet loss rate is high because comparing than RSVP but delay time was very low.

증발잔류 광물의 광물학적 연구

박천영,박신숙,김성구,조갑진,임성수 조선대학교 에너지.자원신기술연구소 2003 에너지·자원신기술연구소 논문지 Vol.25 No.1

This study has focused on mineralogical and geochemical characteritics of evaporated minerals from the mine waters and on variation of constituents in mine water during the evaporation The evaporated minerals are shown in mine waters at the field. In order to form of evaporates in the laboratory, mine waters were sampled from the abandoned mine area(HS, LG and BJ) and were air dried in laboratory for room temperature. During the evaporation of mine waters, TDS, EC values and the concentration of major and minor ions increased, whereas dissolved oxygen(DO) values decreased with the sampling time. Gypsum, epsomite, kaolinite, dolomite and illite were identified in BJ sample, gypsum and epsomite were observed in LG sample, and gypsum, epsomite and vermiculite were founded in HS sample by x-ray powder diffraction studies. The infrared spectra for evaporated minerals show major absorption banded due to OH stretching, adsorbed molecular water and sulfate stretching, respectively. Evaporated minerals were heated at 65, 150, 250 and 350℃ for 1 hour in electrical Furnaces. Gypsum transformed the bassanite at 150℃ heating temperature and epsomite transformed the hexa-hydrite at 65℃, and then converted the kieserite at 250℃. With increasing heating temperature, the intensity of absorption OH band(3407 11㎝^(-1)), adsorbed molecular water band (1654.39㎝^(-1)) and sulfate stretching band (1113.46㎝^(-1)) were decreased, whereas sulfate stretching bands(668.35㎝^(-1) and 603.85㎝^(-1)) were relatively increased in IR due to dehydration.

항균성 동치미액의 첨가에 의한 냉면국물 중의 Salmonella typhimurium 및 Staphylococcus aureus 생육억제

소명환,조신호,이재우,이효구 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.2

동치미액은 전통적으로 냉면국물로 이용되어왔다. 냉면국물의 미생물 오염문제를 해결하기 위하여 항균활성이 높은 김치 젖산균 Lactobacillus homohiochii B21과 Leuconostoc mesenteroides subsp. mesenteroides C16을 스타터로 혼합사용하여 동치미액을 제조하고, 이를 냉면국물에 100%, 50%, 10% 및 0% 첨가하여 20℃ 및 10℃에서 보관할 때에 동치미액의 항균력으로 인하여 의도적으로 첨가한 Salmonella typhimurium과 Staphylococcus aureus의 증식이 억제되는 정도를 조사하였다. 동치미액 100%인 냉면국물은 20℃에서 보관할 때는 Salmonella typhimurium은 16시간만에, Staphylococcus aureus는 32시간만에 10^6CFU/ml에서 10^0CFU/ml로 각각 급격히 사멸하였고, 10℃에서 보관할 때도 시간경과에 따라 생균수가 급격히 감소하였으나 감소속도는 20℃의 경우보다 느렸다. 동치미액을 50% 첨가한 육수에서도 Salmonella typhimurium과 Staphylococcus aureus는 시간경과에 따라 급격히 감소하였으며, 감소속도는 동치미액 100%일 경우보다 느렸다. 동치미액을 10% 첨가한 육수에서는 20℃에서 보관할 때는 초기에 Salmonella typhimurium과 Staphylococcus aureus의 균수증가가 약간 있었으나 동치미액 무첨가에 비하여 현저히 억제되었으며, 8시간∼16시간 이후부터는 균수가 서서히 감소하였다. 육수에 동치미액을 첨가하지 않았을 경우에는 20℃에서 보관할 때는 초기부터 Salmonella typhimurium과 Staphylococcus aureus의 균수증가가 급격히 이루어졌으며, 10℃에서 보관할 때는 24시간 이후부터 서서히 증가하였다. Salmonella typhimurium과 Staphylococcus aureus에 대한 본 동치미액의 억제력은 후자의 미생물보다 전자에 대하여 더 강하게 나타났다. 본 연구의 동치미액을 육수에 50% 첨가하여 냉면국물을 제조한다면 보관중의 미생물 오염문제 해결에 큰 도움이 될 것으로 판단된다. Juice of Dongchimi, a Korean traditional vegetable food fermented with lactic acid bacteria, has been traditionary used as broth for Naengmyon, a Korean cold noodles with broth. This study was carried out to demonstrate the growth inhibition of two food poisoning bacteria, Salmonella typhimurium and Staphylococcus aureus, in Naengmyon-broth containing Dongchimi-juice fermented with high antibacterial lactic acid bacteria, Lactobacillus homohiochii B21 and Leuconostoc mesenteroides C16. Naengmyon-broths were made with beef broth and Dongchimi-juice fermented with lactic acid bacteria, and the changes in viable cell counts of the inoculated food poisoning bacteria in Naengmyon-broths were investigated during storage at 20℃ and 10℃. In Naengmyon-broth of 100% Dongchimi-juice stored at 20℃, the numbers of Salmonella typhimurium and Staphylococcus aureus were rapidly decreased from 10^6CFU/ml to 10^0CFU/ml in 16 hours and 32 hours, respectively. In Naengmyon-broth containing 50% Dongchimi-juice, their numbers were also rapidly decreased, though the decreasing rates were not so fast as those in 100% Dongchimi-juice. In Naengmyon-broth containing 10% Dongchimi-juice, the growths of the two food poisoning bacteria were markedly inhibited, compared with those in 100% beef broth, though some growths were occurred in early phase. But in Naengmyon-broth of 100% beef broth, their growths were very fast from early. Antibacterial activity of the Dongchimi-juice was more distinct at 20℃ than at 10℃, and was more active against Salmonella typhimurium than against Staphylococcus aureus.