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      • KCI등재

        Dielectric and piezoelectric properties of MeTiO3 (Me=Ba and Sr) modified (K, Na)NbO3 potassium-sodium niobate ceramics

        Yoshiro Shimojo,Kunio Matsuzaki,Ruiping Wang,Tadashi Sekiya 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.1

        To develop lead-free piezoelectric materials, (1 { x)(K0:6Na0:4)NbO3-xBaTiO3 and (1x)(K0:6Na0:4)NbO3-xSrTiO3 solid solutions were prepared by the hot-press method. The dielectric and piezoelectric properties of the samples were investigated as a function of dopant content x and type of dopant ions. It is found that the ferroelectric phase transition temperatures are dominated by the x value and the piezoelectric properties depend on both x and the type of dopant ions. The reasons for the results are discussed.

      • KCI등재

        In Vitro Performance of Injectable Chitosan-Tripolyphosphate Scaffolds Combined with Platelet-Rich Plasma

        Andréa Arruda Martins Shimojo,Sofia Elisa Moraga Galdames,Amanda Gomes Marcelino Perez,Thiago Heiji Ito,Ângela Cristina Malheiros Luzo,Maria Helena Andrade Santana 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.1

        This study aimed to evaluate the in vitro biological effectiveness of chitosan microparticles crosslinked with sodium tripolyphosphate (TPP) in combination with activated pure platelet-rich plasma (aP-PRP) as an injectable composite scaffold for growth factors release, cell proliferation and osteogenic differentiation. Two main novelties were addressed in the field of scaffolds in regenerative medicine: the first is the approach including simultaneously the three vertices of the proliferation triangle formed by the capabilities genic progenitor cells, conductive scaffolds and inductive growth factors, which are provided by platelet rich plasma (PRP); secondly, the approach of an injectable composite scaffolds formed by the fibrin network from aP-PRP and the chitosan microparticles crosslinked with TPP. The microparticles were prepared by vortexing the chitosan and TPP solutions. The ionic crosslinking of chitosan with TPP was made at mass ratios of 2:1, 5:1, and 10:1 at pH 4.0. P-PRP was obtained via the controlled centrifugation of whole blood. The composite scaffolds were prepared by adding the microparticles to immediately activated P-PRP. The results showed that the microparticles had adequate physicochemical and mechanical properties for injection. Furthermore, the microparticles controlled the release of growth factors from P-PRP. The proliferation of human adipose-derived mesenchymal stem cells was lower than in aP-PRP alone but significant at a 2:1 chitosan-TPP mass ratio. Osteogenic differentiation was stimulated at all studied mass ratios, as indicated by the alkaline phosphatase activity. These results offer perspectives for optimizing the composite scaffold, and to prove its potential as an injectable scaffold in regenerative medicine.

      • SCIESCOPUSKCI등재

        Amino Acid Profiles of Tropical Legumes, Cooper (Glycine wightii), Tinaroo (Neonotonia wightii) and Siratro (Macroptilium atropurpureum), at Pre-blooming and Blooming Stages

        Tokita, Norio,Shimojo, Masataka,Masuda, Yasuhisa Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5

        An experiment was conducted to investigate amino acid composition of three tropical legumes (Cooper (Glycine wightii), Tinaroo (Neonotonia wightii) and Siratro (Macroptilium atropurpureum)) at two different stages (pre-blooming and blooming stages). Chemical composition and totally 16 amino acids of these plants were analysed for comparison of their composition among species at different growing stages and characterizing the amino acid pattern of these legumes. Crude protein content of the plants ranged from 16% to 27% on a dry matter basis. The total amount of 16 amino acids analyzed in this experiment was highest at 89.7 mg/16 g N in Cooper at pre-blooming and lowest at 80.9 mg/16 g N in Glycine at blooming stage. Total amount of amino acids in each legume species tended to slightly decrease with their maturity but no statistical difference was found. The percentage of aspartic acid, glutamic acid and proline in the total amount of amino acids was dominant at 9% to 13%, and that of methionine was less than 1.6%. In this experiment it was concluded that three tropical legumes were rich in crude protein content and characterized by 16 different amino acids with lower sulfur-containing amino acid as methionine.

      • SCIESCOPUSKCI등재

        IMPROVEMENT IN THE NUTRITIVE VALUE OF RICE STRAW BY TREATMENT WITH THE URINE OF GOATS

        Hasan, S.,Shimojo, M.,Goto, I. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.1

        This study was conducted to measure the improvement in nutritive value of rice straw by treatment with urine of goat under different temperatures. Straw samples were japonica I (comprising 15 varieties with culms longer than 123 cm and mean silica content of 13.0%), japonica II (comprising 20 varieties with culms less than 87 cm and mean silica content of 13.7%), indica I (Java 14 variety with a high content of silica of 13.9%) and indica II (comprising IR-8 and IR-20 varieties with a low content of silica of 11.0%). Urine was collected from four Japanese Saanen goats fed alfalfa hay. The different straws were sprayed with urine at the rate of 1 ml/g straw DM, put in double layer plastic bags, sealing and stored for 4 weeks at either $20^{\circ}C$ (GU-20) or $40^{\circ}C$ (GU-40). The in vitro dry matter digestibility (IVDMD) and organic matter digestibility (IVOMD) of the straws were improved by the GU-40 treatment (13.5% and 13.2%, but the GU-20 treatment had little effect of digestibility (1.5% and -0.2%) except with the low silica indica II varieties. The GU-40 treatment tended to decrease NDF and hemicellulose content of straws. Crude protein content of the straws was improved by both the GU-20 and GU-40 treatments. It was concluded that treatment of rice straw with goat urine at $40^{\circ}C$ for 4 weeks improved the crude protein content and digestibility in vitro, the greatest improvement occurring with straws of lowest digestibility in the untreated state.

      • SCIESCOPUSKCI등재

        Effect of Additives on the Fermentation Quality and Residual Mono- and Disaccharides Compositions of Forage Oats (Avena sativa L.) and Italian Ryegrass (Lolium multiflorum Lam.) Silages

        Shao, Tao,Shimojo, M.,Wang, T.,Masuda, Y. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.11

        This study aimed to evaluate the effects of silage additives on the fermentation qualities and residual mono- and disaccharides composition of silages. Forage Oats (Avena sativa L.) and Italian Ryegrass (Lolium multiflorum Lam.) were ensiled with glucose, sorbic acid and pre-fermented juice of epiphytic lactic acid bacteria (FJLB) treatments for 30 days. In both species grass silages, although the respective controls had higher contents of butyric acid (20.86, 33.45g $kg^{-1}$ DM) and ammonia-N/total nitrogen (100.07, 114.91 g $kg^{-1}$) as compared with other treated silages in forage oats and Italian ryegrass, the fermentation was clearly dominated by lactic acid bacteria. This was well indicated by the low pH value (4.27, 4.38), and high lactic acid/acetic acid (6.53, 5.58) and lactic acid content (61.67, 46.85 g $kg^{-1}$ DM). Glucose addition increased significantly (p<0.05) lactic acid/acetic acid, and significantly (p<0.05) decreased the values of pH and ammonia-N/total nitrogen, and the contents of butyric acid and volatile fatty acids as compared with control, however, there was a slightly but significantly (p<0.05) higher butyric acid and lower residual mono- and di-saccharides as compared with sorbic acid and FJLB additions. Sorbic acid addition showed the lowest ethanol, acetic acid and ammonia-N/total nitrogen, and highest contents of residual fructose, total mono- and di-saccharides and dry matter as well as high lactic acid/acetic acid and lactic acid content. FJLB addition had the lowest pH value and the highest lactic acid content, the most intensive lactic acid fermentation occurring in FJLB treated silages. This resulted in the faster accumulation of lactic acid and faster pH reduction. Sorbic acid and FJLB additions depressed clostridia or other undesirable bacterial fermentation, thus this decreased the water-soluble carbohydrates loss and saved the fermentable substrate for lactic acid fermentation.

      • SCIESCOPUSKCI등재

        CHEMICAL COMPONENTS INFLUENCING LODGING RESISTANCE OF RICE PLANT AND ITS STRAW DIGESTIBILITY IN VITRO

        Hasan, S.,Shimojo, M.,Goto, I. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.1

        This study was conducted to investigate the chemical components of culm that influencing the resistance to lodging and the in vitro digestibility in indica type rice plants. Indica type rice plants with three levels of lodging resistance were used; resistant type (RT), intermediate type (IT) and susceptible type (ST). For each type there were four varieties. Culm length was shorter in RT and longer in ST (98.1 cm vs. 151.8 cm). Silica content in the culm was highest in RT and lowest in ST (11.0% vs. 7.6%). There was no difference in the level of acid detergent lignin (ADL) between the 3 levels of lodging. For the 12 straw samples, in vitro organic matter digestibility (IVOMD) was negatively correlated with silica content (r = -0.664, p<0.05) but ADL had no significant effect (r = -0.454, p>0.05). Digestibility was more closely correlated with the sum of silica and ADL (r = -0.747, p<0.01) than silica alone. It was concluded that rice plants showed resistance to lodging when the culm was short and its structure was reinforced with larger quantities of silica. However these silicified straws were less digestible.

      • SCISCIESCOPUS

        Overlapping responses for the expectation of juice and money rewards in human ventromedial prefrontal cortex.

        Kim, Hackjin,Shimojo, Shinsuke,O'Doherty, John P Oxford University Press 2011 Cerebral cortex Vol.21 No.4

        <P>Although much is known about the neural substrates of reward, the question of whether expectation of different types of reinforcers engage distinct or overlapping brain circuitry has not been addressed definitively. In the present study, human subjects, while being scanned with functional magnetic resonance imaging, performed a simple reward-based action selection task to obtain different magnitudes of either monetary outcomes (winning or losing money) or juice outcomes (pleasant apple juice or an unpleasant salt flavor). At the group level, we found partially overlapping value-related activity within ventromedial prefrontal cortex (vmPFC) during anticipation of juice and money reward outcomes. Analogous results were found in the right anterior insula, except that this region showed negative correlations as a function of increasing expected reward. These results indicate that vmPFC and anterior insula contain overlapping representations of anticipatory value, consistent with the existence of a common currency for the value of expected outcomes in these regions.</P>

      • SCIESCOPUSKCI등재

        Effects of Adding Urea and Molasses on Napiergrass Silage Quality

        Yunus, M.,Ohba, N.,Shimojo, M.,Furuse, M.,Masuda, Y. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.11

        To standardize proper formulation of urea and molasses, the former to increase crude protein content of tropical grass and the latter for improving its silage quality, we examined the fermentation quality of silage of fresh and wilted napiergrass (Pennisetum purpureum Schumach) with different levels of urea and molasses with or without lactic acid bacteria (LAB). Silage was made of napiergrass with conditions of fresh young (Exp. 1),young wilted for half day (Exp. 2) and fresh mature (Exp. 3). Chopped plant materials of about 1cm length were ensiled into a laboratory silo and incubated for one month at $25^{\circ}C$. The treatments were the combination of 0, 0.2 and 0.6% of urea and 0, 2 and 5% of molasses (fresh material basis) with or without LAB inoculation. After opening the silo, pH, organic acids, volatile basic nitrogen (VBN) and total nitrogen (TN) were determined. Addition of molasses significantly (p<0.01) lowered pH values in three experiments. Though molasses addition increased lactic acid production even at a higher level of urea, pH values at 0 and 2% molasses were significantly increased by urea in fresh and wilted young silages, but in fresh mature silage it occurred only when molasses was not added. VBN/TN at 0.6% urea were decreased significantly by the highest molasses in three experiments. Significant increases in TN by the increasing of urea addition were observed at all levels of molasses in wilted young and fresh mature silages. In conclusion, a combination of 5% molasses and 0.6% urea could improve the nutritive and fermentation qualities of napiergrass silage under young, wilting and mature conditions.

      • SCIESCOPUSKCI등재

        Dynamics of Early Fermentation of Italian Ryegrass (Lolium multiflorum Lam.)Silage

        Shao, Tao,Ohba, N.,Shimojo, M.,Masuda, Y. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.11

        The dynamics of fermentation were studied with Italian ryegrass ensiled in the laboratory silos. The silos were kept in the room set at 25$^{\circ}C$, and then were opened on 0.5, 1, 2, 3, 5, 7 and 14 days after ensiling, respectively. The samples were taken from three silos at each sampling time for chemical analyses. Mono-and disaccharides composition was determined for glucose, fructose and sucrose by high performance liquid chromatography. The Italian ryegrass silage succeeded to achieve lactate type fermentation; high values of lactic acid (85.83 g/kg) and lactic acid/acetic acid at the end of ensiling (14 day), low values of pH (3.74), acetic acid (5.38 g/kg), ethanol (19.20 g/kg) and $NH_3-N/Total\;N$ (75.84 g/kg), no or only small amounts of butyric acid, valeric acid and propionic acid. The fermentation dynamics showed a fast and large pH decrease caused by a fast and large production of lactic acid during the first 5 days. Mono-and disaccharides composition largely decreased within initial 0.5 day (12 h) of ensiling. Sucrose disappeared rapidly within initial 0.5 day of ensiling, and fructose and glucose contents showed an initial rise during ensiling, and then decreased gradually. These indicated that the enzymes of plant tissue were active within 2 days of ensiling, which caused the initial rise in fructose and glucose from the hydrolysis of sucrose and fructans. After 5 days of ensilage, glucose was consumed completely, suggesting that glucose was the first fermentation substrate. After 2 days of ensiling, sum amounts of lactic acid and remaining mono-and disaccharides proved to be larger than the quantity of mono-and disaccharides in the initial grass. From the facts mentioned above, it was suggested that considerable amounts of lactic acid were produced from some other substrate such as fructans than initial mono-and disaccharides.

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