Effect of Forging Type on the Deformation Heterogeneities in Multi‑Axial Diagonal Forged AA1100

Min‑Seong Kim,Sang‑Chul Kwon,Sun‑Tae Kim,Seong Lee,Hyo‑Tae Jeong,Shi‑Hoon Choi 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3

The present study investigated the effects that different types of forging exert on the deformation heterogeneities developedin AA1100 during multi-axial diagonal forging. To measure the deformation heterogeneities of deformed workpieces, thevalues for hardness and Kernel average misorientation were measured at the center section following each forging process. Type-D forging that consists of diagonal forging and return-diagonal forging was relatively advantageous compared withType-P forging that includes plane forging and return-plane forging for minimizing the non-uniformity of deformationdeveloped in workpieces. The effective strain developed in a workpiece during the 2 types of forging was simulated using3-D FEA. FEA revealed that the positions and degrees of occurrence for soft and hard-zones in workpieces vary greatlydepending on the forging type. Type-D forging was relatively advantageous compared with Type-P forging for minimizingthe non-uniformity of effective strain developed in workpieces.

단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

생활원예 행태에 관한 연구 : 천안시를 중심으로

이애경,도경민,서정근 韓國植物ㆍ人間ㆍ環境學會 1999 인간식물환경학회지 Vol.2 No.3

This study was conducted to investigated the status of horticultural working of Cheonan, Korea. Most of the house had flowers and vegetables garden which was located of gate along the wall and external house. Most of horticultural working participants was women(66%) and age brackets was above 50(72%). The space of horticultural working was internal house(38%) and external house(12%). Cultivated plants in garden were mainly vegetables(30.1%), deciduous tree(25.7%), and evergreen tree(13.5%), plants were cultivated in vacant area(40%) and in weekend farm(16%). Participants were satisfied in their garden, but they required the knowledge of horticultural working(51.9%), So participants of horticultural working required horticultural knowledge, skill and programs by a specialist or governments.

臨床檢體에서 分離된 E. coli의 臨床細菌學的 調査硏究

申鉉成,吳是煥,李敏雄 대한화학요법학회 1986 대한화학요법학회지 Vol.4 No.2

A total of 25004 cases of clinical materials from patients was collected in H hospital from January 1974 to December 1984 in Seoul. Among the materials, 7773 cases of the clinical specimens were identified as pathogenic, organisms. The numbers of cases of E. coli isolated from the specimens were 1838 except for cases of incubation of human feces. Infectious pattern and antibiotics sencitivity of the pathogenic organisms and E. coli were as follows: Total clinical specimens, and isolation rate of E. coli from total clinical materials and to the identified pathogenic organisms were increased more than 2 times in 1982 to 1984 comparing to the year in 1974 to 1976. The range of percentage of all identified microorganisms to total clinical specimens was 27.0~34.6% yearly. Isolated ratio E. coli to total specimens was 7.4% and the ration of isolated E. coli to total isolated pathogens was 23.6%(1838/7773). The isolation ratio of E. coli per each patient was 1.323 for male and 1.21 for female. The isolation ratio-of male to female was 1 : 1.88. The highest number of E. coli was found in May for male and June for female according to monthly isolation. On the other hand the lowest number of E. coli was observed in February for male and October for female respectively. In seasonal fluctuation of the isolated orgainsms from patients specimens, the isolation rate was maximized in fall for male, and in summer for female, but the ratio was generally decreased in winter season. The age specific isolation rate of E. coli was 27.8% under 10 year old group for male and 27.1% in 20~29 year old group for female, but the frequency of the organisms was observed lower in 10~19 year old group in both sexes. The isolating sources of specimens in male were 41% in pus, 22.2% in urine and 20.1% in throat swab, and the sources in female were 59.7% from urine, 14% in pus and 11.3% from vaginal discharge. The susceptibility to antibiotics studied for 11 years was all less than 40% in streptomycin, cephalothin, chloramphenicol, carbenicillin, tetracyclin, ampicillin, erythromycin and penicillin except for 73.6% in gentamicin and 52.8% in kanamycin. But in the last 2 years, the antibiotics showing over 90% sensitivity against E. coli were cefotaxime, sisomicin, cefoxitin, amikacin and pipemidic acid.

Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin

Cheng Baixiang,Feng Fan,Shi Fan,Huang Jinmei,Zhang Songbai,Quan Yue,Tu Teng,Liu Yanli,Wang Junjun,Zhao Ying,Zhang Min 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4

BACKGROUND: Although newly formed constructs of feasible pressure-preadjusted bone marrow mesenchymal stem cells (BMSCs) and platelet-rich fibrin (PRF) showed biomechanical flexibility and superior capacity for cartilage regeneration, it is still not very clear how BMSCs and seed cells feel mechanical stimuli and convert them into biological signals, and the difference in signal transduction underlying mechanical and chemical cues is also unclear. METHODS: To determine whether mechanical stimulation (hydrostatic pressure) and chemical cues (platelet-rich fibrin, PRF) activate canonical or noncanonical Wnt signaling in BMSCs, BMSCs cocultured with PRF were subjected to hydrostatic pressure loading, and the activation of the Wnt signaling molecules and expression of cartilage-associated proteins and genes were determined by western blotting and polymerase chain reaction (PCR). Inhibitors of canonical or noncanonical Wnt signaling, XVX-939 or L690,330, were adopted to investigate the role of Wnt signaling molecules in mechanically promoted chondrogenic differentiation of BMSCs. RESULTS: Hydrostatic pressure of 120 kPa activated both Wnt/b-catenin signaling and Wnt/Ca2? signaling, with the the maximum promotion effect at 60 min. PRF exerted no synergistic effect on Wnt/b-catenin signaling activation. However, the growth factors released by PRF might reverse the promotion effects of pressure on Wnt/Ca2? signaling. Real-time PCR and Western blotting results showed that pressure could activate the expression of Col-II, Sox9, and aggrecan in BMSCs cocultured with PRF. Blocking experiment found a positive role of Wnt/b-catenin signaling, and a negative role of Wnt/ Ca2? signaling in chondrogenic differentiation of the BMSCs. Mutual inhibition exists between canonical and noncanonical Wnt signaling in BMSCs under pressure. CONCLUSION: Wnt signaling participates in the pressure-promoted chondrogenesis of the BMSCs co-cultured with PRF, with canonical and noncanonical pathways playing distinct roles during the process.

퉁퉁마디로부터 색소체 외막 단백질 유전자의 분리 및 발현분석

네티 엘마와티,차준영,양영실,정민희,신동진,이병현,이곤호,손대영 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na+ sensitivity of yeast mutant (cnbΔ) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.

Activation by Combined Treatment with Cyclobeximide and Electrical Stimulation of In-Vitro Matured Porcine Oocytes Improves Subsequent Parthenogenetic Development

Naruse, Kenji,Kim, Hong Rye,Shin, Young Min,Chang, Suk Min,Lee, Hye Ran,Tarte, Vaishali,Quan, Yan Shi,Kim, Beak Chul,Park, Tae Young,Choi, Su Min,Park, Chang Sik,Jin, Dong Il 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Electrical treatment has been widely used for porcine oocytes activation However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10 μg/ml) for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to 6.25 μM calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to 10 μg/ml cycloheximide and electrical pulse treatment was significantly increased (P<O.05) than in the control group. And exposure to 6.25 μM calcium ionophore for 2 min with 10 μg/ml cycloheximide for 10min and electrical pulse treatment significantly increased (P<O.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.

Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Oocytes Improves Subsequent Parthenogenetic Development

Kenji Naruse,Hong Rye Kim,Young Min Shin,Suk Min Chang,,Hye Ran Lee,Vaishali Tarte,Yan Shi Quan,Beak Chul Kim,Tae Young Park,Su Min Choi,Chang Sik Park,Dong Il Jin 사단법인 한국동물생명공학회 2006 Reproductive & developmental biology Vol.30 No.1

Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10 μg/ml) for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to 6.25 μM calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to 10 μg/ml cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to 6.25 μM calcium ionophore for 2 min with 10 μg/ml cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.

Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Porcine Oocytes Improves Subsequent Parthenogenetic Development

Naruse Kenji,Kim Hong-Rye,Shin Young-Min,Chang Suk-Min,Lee Hye-Ran,Tarte Vaishali,Quan Yan-Shi,Kim Beak-Chul,Park Tae-Young,Choi Su-Min,Park Chang-Sik,Jin Dong-Il 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.1

Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10μgml) for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to 6.25μM calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to 10μgml cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to 6.25μM calcium ionophore for 2 min with 10μgml cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development

Influence of LPPS Spraying Parameters on Deposition Efficiency of Zirconia Powder

Shi, Jian-Min,Hu, Zhong-Yin,Huang, Jing-Qi,Ding, Chuan-Xian The Korean Vacuum Society 1997 Applied Science and Convergence Technology Vol.6 No.1

Yttria stabilized zirconia coating is an attractive material for several engineering applications. In order to produce coatings with consistent and reliable performance it is important to understand the influence of spraying parameters on the coating properties and optimize the spraying parameters. In this paper the low pressure plasma spray(LPPS) deposition of as-received zirconia powder has been investigated using simple one-factor-at-a-time approach. The deposition efficiency was chosen to evaluate the melting characteristics of the as-received zirconia powder. The results obtained indicated that the deposition efficiency of zirconia powder is very sensitive to the spraying parameters such as plasma gas flow rate and ranges from 24% to 57% The microstructure and the phase composition of zirconia coating deposited with the different plasma spraying parameters were also examined by SEM and XRD respectively. The relationship between deposition efficiency and the microstructure of zirconia coating was discussed.