Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

남한 내 북한이탈주민의 3년간 사회적응 추적 연구 : 2001년부터 2004년까지 생활과 교육을 중심으로 With prioty given to life of North Korea defectors from 2001 to 2004

유시은,전우택,조영아,홍창형,엄진섭 연세대학교 통일연구원 2005 통일연구 Vol.9 No.1

Purpose of this study : In 2001, this study that investigate actual conditions about adaptation degree of Korean social life of the North Korean defectors was carried out to 151 people that succeed in chases among 200 the North Korea defectors answer in study. Purpose of this study is as following. First, we wished to search change aspect of actual conditions about South Korea society adaptation of the North Korean defectors who take part in the first investigation for past 3 years. Second, we wished to search difference of adaptation aspect by populational and social element. Third, We wished to suggest things that can be beaconed to efficient support policy of government or private organization and successful South Korea social adaptation method which support the North Korean defectors on the basis of study finding about change of adaptation aspect of the North Korea defectors. Methods of Study : We designated 200 Panels to study and survey actual adaptation conditions of the North Korean defectors on South Korea society synthetically and for long-term. So, chasing about 151 people was possible in 2004. We are fertilized and supplement the questionnaire forming in 2001 and composed the new questionnaires to part of a life, mental state, Body and health. This study among them investigated a standard of living of the North Korean defectors. In this process, interviewer directly asked and recorded questions to reduce individual variation of people who receive interview. Study Results : Government's residence support policy about North Korean secession inhabitants was generally attained as effective and suitable for North Korean inhabitants' early South Korea settlement. Only, government need to discuss about North Korea secession inhabitants' residence support more variously for long-term. Second, education should be supported more actively and variously. Suitable point of education for their needs should make a effect of vocational training or education maximize. This is a very important part. Third, they need guidance and education that can receive more easily law support. Fourth, medical supports and health cares should be achieved continuously and systematically to them since they arrived in South Korea. To supports and studies North Korean secession inhabitants has the large significance to gain a foothold about reunification of North and south. Their South Korea society adaptation is becoming topic of conversation among South Korean. An atmosphere of unity is elated. And interest degrees about North Korean are actually rising as exchanges between South and North Korean is activated. Therefore, the adaptation study about North Korean secession inhabitants will have to be expanded in studies for South Korean, not to be limited in studies for North Korean.

계태에서의 31P 자기공명분석법을 이용한 인대사변화의 추적

홍창의,임태환,이정희,이대근,유시준 울산대학교 의과대학 1994 울산의대학술지 Vol.3 No.2

Chick embryo is a convenient model for embryologic teratologic studies. We performed the present experimental study to establish the basic technology for magnetic resonance studies of chick embryos and to examine the biochemical changes occurring during the development of chick embryos. Fertilized Leghorn eggs were examined by 31-phosphours magnetic resonance spectroscopy (??P-MRS) on days 6, 9, 12, 14, 15, 17 and 20 after the start of incubation. Spectroscopic data of live embryos were acquired by using a 4.7T MR spectrometer, and signals from phosphorous metabolites were analyzed. Total phospholipid signal decreased, which resulted in the overall decrease in total phosphorous signal over time. Signals from other metabolites such as ATPs and phosphocreatine increased over time, while those from inorganic phosphate and sugar phosphates decreased. Before the incubation days 12, signals from sugar phosphates and inorganic phosphate were predominant, and other metabolites became remarkably functional between the days 12 and 14 when the decrease in the phospholipid signal was significant. MR specrtroscopy is an effective method in evaluation of the biochemical changes occurring during the developmental period of chick embryos.

친환경 건축물 인증이 부동산 거래가격에 미치는 영향 분석: 주거용 부동산과 상업용 부동산의 비교를 중심으로

최시영 ( Choi Shi-young ),정창무 ( Jung Chang-mu ),김현정 ( Kim Hyun-jung ) 한국부동산분석학회 2022 不動産學硏究 Vol.28 No.1

도시에서 배출되는 탄소와 온실가스는 기후변화에 큰 영향을 미친다. 기후변화를 방지하기 위해 전 세계적으로 친환경 건축물 보급을 위해 노력하고 있으며 친환경 인증 건축물이 부동산 가격에 미치는 영향에 대한 연구도 진행되었다. 본 연구는 친환경 건축물이 부동산 가격에 미치는 영향을 건축물의 용도에 따라 비교하기 위하여 진행되었다. 이에 따라 본 연구는 서울시 강서구의 주거용 부동산(아파트)과 상업용 부동산(상가 · 사무실)을 대상으로 에너지효율등급을 부여받은 건축물이 매매가격에 미치는 영향의 정도를 비교 분석하였다. 이를 위해 평당 거 래가격을 종속변수로 두어 다중회귀 분석의 형태를 띤 해도닉가격모형 분석을 수행하였다. 실증분석 결과, 매매 가격에 에너지효율등급이 미치는 영향력의 정도는 상업용 부동산(β = 0.045)보다 아파트(β = 0.537)에서 더 높게 나타났다. 본 연구는 친환경 건축물이 단순히 매매가격에 영향을 미치는지에 대해 분석한 선행연구들과는 달리 건물의 용도에 따라 영향정도를 비교한 연구라는 데에 의의가 있다. 향후 연구에서는 데이터센터, 오피스텔, 물류 시설, 공장 등 보다 다양한 형태의 건물이 부동산 시장에 미치는 영향을 연구할 필요가 있다. 또한 지역의 특성이나 계절에 따른 에너지효율등급의 실효성 연구 등 다양한 관점에서 친환경 건축물의 실요성에 대해 분석할 필요가 있다. 본 연구결과를 기반으로 전략적인 친환경 건축물 공급을 유도할 수 있다는 것으로서 그 가치가 있다. Pollutants and carbon emitted from cities have a major impact on global warming and climate change. Considerable efforts are being made to supply eco-friendly buildings to reduce greenhouse gases emitted from buildings worldwide. In addition, numerous studies have shown that eco-friendly building certification affects real estate prices. The purpose of this study is to examine the effects of eco-friendly buildings on real estate prices spanning different types of buildings. This study investigated the relationship between energy-efficiency ratings and real estate prices for residential buildings and commercial property in Gangseo-gu, Seoul, Korea using the Hedonic price model analysis. The influence of energy-efficiency rating on real estate prices was found to be greater on residential buildings (β=0.537) than that on commercial properties (β=0.045). This study expands from the previous studies that simply analyzed whether eco-friendly buildings had an effect on real estate prices by comparing the degree of influence on different building types. Future research needs to study the impact of more diverse types of buildings, such as data centers, officetels, logistics facilities, and factories, etc. on the real estate market beyond residential and commercial buildings. In addition, studies comparing the effect of eco-friendly buildings on prices depending on the characteristics of the region or the effectiveness of season-dependent energy efficiency grades may be conducted. Based on the results of this research, development strategies can be established in the private sector, and the public sector can make policies to induce the private sector to provide more eco-friendly buildings.

신경외과 영역에서의 접착성 지혈제가 주변뇌조직에 미치는 영향에 대한 실험적 연구

장헌상,김관태,송시헌,김성호,김윤 대한신경외과학회 1991 Journal of Korean neurosurgical society Vol.20 No.10-11

In a series of 20 young rats, three cortical lesions were made in each hemisphere. Two of these lesions were filled with avitene and gelfoam, while the third was left empty as a control. The animals were killed successively on weeks 1, 2, 4 and 8 after the operation. The results were as follows : 1) Although there was no difference in the type of tissue reactions, avitene was more rapid and profound than gelfoam in the process and degree of inflammatory reaction at the same periods. 2) Avitene biodegradaded more rapidly than gelfoam. 3) The extent of fibrosis and adhesion to the surrounding brain tissues were not grossly different between these two agents but more profound histopathologically in avitene at the same periods. With these results, we could conclude that the avitene was superior to the gelfoam as the hemostatic agent in neurosurgical area.

단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

쓰레기 매립지의 안정화처리에 관한 연구(I) : 침출수 및 우수처리

권창오 ; 차시환 김천대학교 1995 김천대학교 논문집 Vol.16 No.-

This study is an experimental research on the stabilization of landfill of the domestic solid waste. The purpose of this study is to investigate the environmental aspects which are accompanied by carrying and landfilling the domestic into the Changsin-dong Young ju city landfill site. In order to know the hydrogeolosical condition, we carried out the multipurpose experimental excavation for surveying the groundwa-ter level, hydraulic conductivity of aquifer and taking the natureal soil samples. The experiment was done by in-situ and instrumental analysis for soil in order to evaluate the physi-co-chemical characteristics of the solid.

Microcapsule의 효소고정화에 따른 CSTR 에서의 물질전달에 관한 연구(II)

차시환 ; 권창오 김천대학교 1998 김천대학교 논문집 Vol.19 No.-

Polyamide capsules containing aqueous solution of GOD were prepared by the interfacial polymerization of ethylenediamine and terephthaloyl chloride, and were coated with the bilayer membrane of dialkyldimethylammonium bromide(2CnN+2C1, n=11, 12, 13, 15). The activity control of the immobilized GOD through the capsule membrane was investigated for the effect of temperatures and pH changes.

신경외과의 수술후 감염증 등 세균에의 cefpiramide sodium(Cefirad^(�))의 효과연구

최창명,송시헌,김관태,김윤 최신의학사 1989 最新醫學 Vol.32 No.4

POCS를 이용한 프레임간 압축 영상의 블록화 현상 제거 방법

정시창,백준기 중앙대학교 정보통신연구소 1999 정보통신연구소논문집 Vol.1 No.1

본 논문에서는 동영상에서 프레임간 예측 방식을 이용하여 압축된 영상(P-프레임)의 블록화 현상을 모델화 하고, 그에 따른 블록화 현상 제거 방법을 제안한다. 동영상의 압축 과정은 전 영상에서 움직임 보상된 영상으로부터 차분 영상을 구하고, 그 차분 영상의 DCT 계수를 양자화 함으로써 압축되게 된다. DCT계수의 블록단위의 양자화 과정과 블록화 현상을 갖는 전 영상으로부터의 움직임 보상 과정에 의하여 P-프레임에는 블록 경계뿐만 아니라, 블록 내부에서도 불연속이 나타나게 된다. P-프레임 압축 영상이 블록화 현상을 갖지 않으려면, 차분 영상은 블록의 경계와 블록 내부에서 움직임 보상된 전 영상의 불연속과 반대방향의 불연속을 가져야 한다. 본 논문에서는 이러한 특징들을 이용하여 차분 영상에서의 철면 집합을 정의하고, POCS이론을 이용한 블록화 현상 제거 방법을 제안한다. In this paper, we propose a degradation model for blocking artifacts in inter-frame coded images and corresponding image restoration algorithm based on convex projection for reducing blocking artifacts. In contrast to still images, inter-frame coded images has two different kinds of blocking artifacts. The one arises on block boundary, which is caused by quantization of block discrete cosine transform (BDCT) coefficients. The other arises on block interior, which is caused by motion compensation from the previous blocky image. These properties are used to define convex sets. Namely, on both block boundary and interior, differential images must have discontinuity in opposite direction to the previous motion compensated image.