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Jeong Sheop Shin(申正燮),Seung Jae Lee(李承再),Kuen Woo Park(朴權瑀) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
A total of 39 genotypes of watermelon were chosen to identify the frequency of RAPD polymorphisms and to group into sub-populations. Of the 15 primers tested, 14 primers except primer 275 showed the polymorphic pattern. A total of 162 bands were generated across all 39 genotypes. Among them, 100(62%) were polymorphic and the remaining 62(38%) were monomorphic. The mean 1-F(genetic distance) was 0.114, and this value corresponded to the mean value of 0.117 detected in 36 genotypes of sorghum. The hightest value was 0.309 and the smallest one was 0.012. The 100 polymorphic fragments of total 162 bands were proved to be very reliable and confident, and selected as useful polymorphic markers. The mean value in this comparison was 0.24 and the highest onewas 0.69. Eight RAPD markers were expected to be utilized in the unique variety discrimination of eight watermelon genotypes. Using DNA polymorphisms, the two major clusters were resolved and one of these was subdivided into three cluster. Each sub-group were characterized and identified again with morphological and genetic characteristics of respective genotype.
Jeong Sheop Shin(申正燮) 한국육종학회 1992 한국육종학회지 Vol.24 No.3
Genomic single or low copy number DNA clones from random genomic DNA libraries using the plasmid vector pBR322 and the phage λgtll were constructed using DNA from a barley cultivar, Sacheon #6. This work was done to develop the barley RFLP DNA markers and to saturate the linkage groups. In the initial screening, 25 clones, which were expected to have single or low-copy sequence, were selected from 1,200 bacterial colonies and 50 genetically unique phage plaques. Of these, 10 clones showed polymorphisms. To do these works, DNAs that were isolated from the barley lines and cultivars, MMS, Apex, Olbori, Sacheon #6, were digested with 3 restriction enzymes, Bam HI, Hind Ⅲ and Eco RI to prepare the genomic blot. Chemiluminescence as well as nick-translation was utilized to label the probe.
식물 Restriction Fragment Length Polymorphism DNA 인자의 이용
신정섭 한국생화학분자생물학회 1991 생화학분자생물학회 소식 Vol.11 No.3
The lack of available genetic markers in cultivated genotypes has limited the development of saturated genetic linkage maps in plant species. Analysis of restriction fragment length polymorphism(RFLP) will provide a relatively unlimited number of genetic markers and permits the construction of detailed genetic linkage maps in eukaryotic species. This review paper here focussed on the previously reported disussions, the general technology and future perspectives for the use of RFLP analysis in the plant species. In basic plant genetics as well as in plant breeding, RFLPs is the promising potent tools.