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Sharma, A.,Lee, B.K. Elsevier 2016 Applied Catalysis A Vol.523 No.-
<P>TiO2 and TiO2-FeO co-doped carbon spheres (CB) synthesized with a suspension polymerization process, followed by carbonization and activation, were used to promote the sorption and photocatalytic removal of toluene from aqueous media. The TiO2 nanoparticles (NPs) and Fe doped TiO2 NPs were incorporated during the middle stage of the suspension polymerization reaction. A porous surface texture on the doped CB was developed by a carbonization and activation process at 800 degrees C under N-2 and CO2 atmospheres, respectively. The doped TiO2-FeO NPs acted as visible-light sensitizers for photo-degradation of toluene under visible light irradiation. The maximum removal capacity of toluene (5-100 mg/L of aqueous solution) was found to be 48.3, 78.1 and 103.1 mg/g, for the TiO2, CB-TiO2 and CB-TiO2-FeO, respectively. The great adsorptive-photo-catalytic process for complete removal of toluene with CB-TiO2-FeO was 98.2%. However, reduction of total organic carbon (TOC) and chemical oxygen demand (COD) was achieved 84.2 and 94.6% respectively, after 3.5 h under visible light irradiation at an initial pH of 3.5. The catalytic removal of toluene followed a pseudo-first-order reaction. The toluene removal in the dark phase indicated that nonpolar toluene molecules are initially sorbed onto the porous structure of CB-TiO2-FeO via electrostatic bonding between nonpolar molecule moieties with phenolic surface of CB-TiO2-FeO. The greatly enhanced photocatalytic performance of CB-TiO2-FeO mainly stemmed from their strong visible-light-harvesting ability of photo-generated electron-hole pairs. (C) 2016 Elsevier B.V. All rights reserved.</P>
Effect of Bradyrhizobium japonicum on Chlorophyll Content, Nodulation, and Plant Growth in Soybean
Roshan Sharma Poudyal,B. N. Prasad 韓國作物學會 2005 Korean journal of crop science Vol.50 No.4
Study on effectiveness of Bradyrhizobium japonicum on soybean [Glycine max (L.) Merr.], local cultivar 'Sathiya' were carried out in the garden soil of Trib-huvan University. Different parameters like nodulation, chlorophyll content in fresh leaves and growth of plant in inoculated and uninoculated plant was studied. Pot experiment was conducted in the green house to evaluate the effectiveness of B. japonicum on soybean. It was observed that B. japonicum inoculation increased the number of nodules, shoot length of plant and total chlorophyll content in fresh leaves of soybean plant. However, root length was decreased in all inoculated plants.
Sankar, S.,Sharma, S.K.,Kaur, N.,Lee, B.,Kim, D.Y.,Lee, S.,Jung, H. Ceramurgica ; Elsevier Science Ltd 2016 CERAMICS INTERNATIONAL Vol.42 No.4
An inexpensive chemical method was used to synthesize biogenic mesoporous silica (m-SiO<SUB>2</SUB>) from rice husk ash (RHA). A comparative study was carried out to produce silica nanoparticles (S-SiO<SUB>2</SUB>, R-SiO<SUB>2</SUB>, and B-SiO<SUB>2</SUB>) from three type of rice husk ashes (sticky, red, and brown). The microstructure of m-SiO<SUB>2</SUB> was dependent on the geographical provenance and the types of RHA. An analysis of the SEM and TEM micrographs reveals that the S-SiO<SUB>2</SUB> nanoparticles had a clustered spherical shape, while R-SiO<SUB>2</SUB> and B-SiO<SUB>2</SUB> nanoparticles were found to be purely spherical. The average crystallite size of S-SiO<SUB>2,</SUB> R-SiO<SUB>2</SUB> and B-SiO<SUB>2</SUB> nanoparticles evaluated from the TEM measurements were observed to be 50, 20 and 10nm, respectively. The XRD pattern of silica nanopowders had an absence of sharp peaks that confirmed the amorphous nature of the material. The Fourier transform infrared (FTIR) spectra of silica nanoparticles showed the symmetric Si-O and O-Si-O stretching bond vibrations at 462, 1088, and 1098cm<SUP>-1</SUP>. The surface area of S-SiO<SUB>2,</SUB> R-SiO<SUB>2</SUB> and B-SiO<SUB>2</SUB> nanopowders was measured to be 7.5513, 201.45, and 247.18m<SUP>2</SUP>g<SUP>-1</SUP>, respectively. The surface area of uniformly-distributed spherical nanoparticles of B-SiO<SUB>2</SUB> was observed the highest, which can be applied for the application of energy storage and drug delivery systems.
Effect of Bradyrhizobium japonicum on Chlorophyll Content, Nodulation, and Plant Growth in Soybean
Poudyal Roshan Sharma,Prasad B. N. The Korean Society of Crop Science 2005 Korean journal of crop science Vol.50 No.4
Study on effectiveness of Bradyrhizobium japonicum on soybean [Glycine max (L.) Merr.], local cultivar 'Sathiya' were carried out in the garden soil of Trib-huvan University. Different parameters like nodulation, chlorophyll content in fresh leaves and growth of plant in inoculated and uninoculated plant was studied. Pot experiment was conducted in the green house to evaluate the effectiveness of B. japonicum on soybean. It was observed that B. japonicum inoculation increased the number of nodules, shoot length of plant and total chlorophyll content in fresh leaves of soybean plant. However, root length was decreased in all inoculated plants.
( T. Selvakumar ),( B. Nataraju ),( K. Chandrasekharan ),( S. D. Sharma ),( M. Balavenkatasubbaiah ),( P. Sudhakara Rao ),( V. Thiagarajan ),( S. B. Dandin ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.11 No.1
While observing silkworm larval samples received from field, microsporidian spores formed within the haemocytes of silkworm haemolymph were observed. The spores of microsporidian sp. were purified and characterized for morphological characters viz., size, shape as well as serological affinity with different Nosema spp. (M11 and M12). The infectivity of the isolated spores to silkworm was also studied. The microsporidian sp. was found to be highly pathogenic to silkworm, B. mori. The isolated microsporidian sp. was designated as NIK-5hm, which formed ovocylindrical spore in the haemocytes of silkworm and differed in spore size (length, 4.55 μm & width, 2.10 μm) and shape from Nosema bombycis (NIK-1s), NIK-2r (Nosema sp. Mysore [3.6 & 2.8 μm]), NIK-3h (Nosema sp. M11 [3.8 & 1.8 μm]), NIK-4m (Nosema sp. M12 [5.0 & 2.1 μm]) and Lb㎳ (Nosema sp. in Lamerine breed of silkworm [4.36 & 2.14]). In immonological test (Latex agglutination test), the isolated microsporidian spores did not react with antibody sensitized latex particles of N. bombycis, M11, M12 and Lb㎳ and thus are different type of microsporidian sp., parasitic to silkworm, Bombyx mori L.
Selvakumar T.,Nataraju B.,Chandrasekharan K.,Sharma S. D.,Balavenkatasubbaiah M.,Sudhakara Rao P.,Thiagarajan V.,Dandin S. B. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.1
While observing silkworm larval samples received from field, microsporidian spores formed within the haemocytes of silkworm haemolymph were observed. The spores of microsporidian sp. were purified and characterized for morphological characters viz., size, shape as well as serological affinity with different Nosema spp. (M$_{11}$ and M$_{12}$). The infectivity of the isolated spores to silkworm was also studied. The microsporidian sp. was found to be highly pathogenic to silkworm, B. mori. The isolated microsporidian sp. was designated as NIK-5hm, which formed ovocylindrical spore in the haemocytes of silkworm and differed in spore size (length, 4.55 $\mu$m & width, 2.10 $\mu$m) and shape from Nosema bombycis (NIK-ls), NIK-2r (Nosema sp. Mysore [3.6 & 2.8 $\mu$m]), NIK-3h (Nosema sp. M$_{11}$ [3.8 & 1.8 $\mu$m]), NIK-4m (Nosema sp. M$_{12}$ [5.0 & 2.1 $\mu$m]) and Lb$_{ms}$ (Nosema sp. in Lamerine breed of silkworm [4.36 & 2.14]). In immonological test (Latex agglutination test), the isolated microsporidian spores did not react with antibody sensitized latex particles of N. bombycis, M$_{11}$, M$_{12}$ and Lb$_{ms}$ and thus are different type of microsporidian sp., parasitic to silkworm, Bombyx mori L.
Yashpal S. Malik,Kuldeep Sharma,Nirupama Vaid,Somendu Chakravarti,K. M. Chandrashekar,Sanjay S. Basera,Rashmi Singh,Minakshi,Gaya Prasad,Baldev R. Gulati,Kiren N. Bhilegaonkar,Awadh B. Pandey 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.3
The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that 39.4% (13/33) of the prevalent RVs were the G3 type while 60.6% (20/33) were dual G3G10 or G3G8 types. P typing revealed that 93.9% (31/33) of the samples were P[11] while 6.1% (2/33) possessed a dual P[1]P[11] type. Sequence analysis of the VP7 gene from G3 strains viz. B-46, 0970, and BR-133 showed that these strains had sequence identities of 90.5% to 100% with other bovine G3 strains. The highest identity (98.9% to 100%) was observed with RUBV3 bovine G3 strains from eastern India. The G3 strains (B-46, 0970, and BR-133) showed 97.5% to 98.8% sequence homologies with the Indian equine RV strain Erv-80. Phylogenetic analysis demonstrated that G3 strains clustered with bovine RUBV3 and J-63, and equine Erv-80 G3. Overall, these results confirmed that the incidence of infection by RVs with the G3 genotype and mixed genotypes in the bovine population was higher than previously predicted. This finding reinforces the importance of constantly monitoring circulating viral strains with the G3 genotype in future surveillance studies.
Malik, Y.P.S.,Chakravarti, S.,Sharma, K.,Vaid, N.,Rajak, K.K.,Balamurugan, V.,Biswas, S.K.,Mondal, B.,Kataria, R.S.,Singh, R.K. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7
Toll-like receptors (TLRs) play an important role in the recognition of invading pathogens and the modulation of innate immune responses in mammals. The TLR4 and TLR7 are well known to recognize the bacterial lipopolysaccharide (LPS) and single stranded (ssRNA) ligands, respectively and play important role in host defense against Gram-negative bacteria and ssRNA viruses. In the present study, coding exon fragments of these two TLRs were identified, cloned, sequenced and analyzed in terms of insertion-deletion polymorphism, within bovine TLRs 4 and 7, thereby facilitating future TLR signaling and association studies relevant to bovine innate immunity. Comparative sequence analysis of TLR 4 exons revealed that this gene is more variable, particularly the coding frame (E3P1), while other parts showed percent identity of 95.7% to 100% at nucleotide and amino acid level, respectivley with other Bos indicus and Bos taurus breeds from different parts of the world. In comparison to TLR4, sequence analysis of TLR7 showed more conservation among different B. indicus and B. taurus breeds, except single point mutation at 324 nucleotide position (AAA to AAM) altering a single amino acid at 108 position (K to X). Percent identity of TLR7 sequences (all 3 exons) was between 99.2% to 100% at nucleotide and amino acid level, when compared with available sequence database of B. indicus and B. taurus. Simple Modular Architecture Research Tool (SMART) analysis showed variations in the exon fragments located in the Leucine Rich Repeat (LRR) region, which is responsible for binding with the microbial associated molecular patterns and further, downstream signaling to initiate anti-microbial response. Considering importance of TLR polymorphism in terms of innate immunity, further research is warranted.
Sankar Ganesh, R.,Sharma, S.K.,Sankar, S.,Divyapriya, B.,Durgadevi, E.,Raji, P.,Ponnusamy, S.,Muthamizhchelvan, C.,Hayakawa, Y.,Kim, D.Y. ELSEVIER 2017 Current Applied Physics Vol.17 No.3
<P>Nanocrystalline BiFeO3 (BFO) powder was synthesized by sol-gel method and subsequent annealed at 100-500 degrees C. The microstructural analysis of BFO nanopowder confirmed the perovskite like structure of spherical nanoparticles annealed at 500 degrees C. The Raman spectrum of BFO nanoparticles showed the rhombohedrally structure with the space group of R3c. The XPS spectrum of BFO nanopowder showed the peaks of Bi, Fe, and O at the binding energy of 158.8, 711.75 and 529.2 eV, respectively. The piezoelectric properties of BFO nanoparticles were evaluated by making the sandwiched structure of graphene/BiFeO3-PDMS/graphene. The fabricated piezoelectric device demonstrated an output voltage of 0.4 V by applying the normal pressure from human finger on the device. (C) 2016 Elsevier B.V. All rights reserved.</P>