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Shuai Shao,Jun Cai,Xin Du,Changgao Wang,Jianguo Lin,Jun Dai 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
Aflatoxin contamination of food and grain poses a serious economic and health problem globally. Aflatoxin B1 (AFB1) is extremely mutagenic and toxic as well as a potent carcinogen to both humans and livestock. In this study, the degradation of AFB1 by extracellular extract of Cladosporium uredinicola was examined using high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and liquid chromatography mass spectrometry. Within 24 h of incubation, AFB1 was efficiently eliminated by the culture supernatant of C. uredinicola (84.5±5.7%) at 37oC; the elimination was proven to be enzymatic, and the enzyme was thermostable. The biotransformation products of AFB1 detected by HPLC and TLC were proven to be the same compound. Analysis with LCMS showed that AFB1 was bio-transformed to a structurally different compound (m/z=365 [M+Na]+), which is first reported. The cytotoxicity study to HeLa cells indicated that culture supernatant-treated AFB1 is less toxic as compared with AFB1.
Shao-shuai Yu,Hai-yan Che,Sheng-jie Wang,Cai-li Lin,Ming-xing Lin,Wei-wei Song,Qing-hua Tang,Wei Yan,Wei-quan Qin 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5
Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loopmediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64oC for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.
Shao, Shuai,Cai, Jun,Du, Xin,Wang, ChangGao,Lin, JianGuo,Dai, Jun 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
Aflatoxin contamination of food and grain poses a serious economic and health problem globally. Aflatoxin $B_1$ ($AFB_1$) is extremely mutagenic and toxic as well as a potent carcinogen to both humans and livestock. In this study, the degradation of $AFB_1$ by extracellular extract of Cladosporium uredinicola was examined using high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and liquid chromatography mass spectrometry. Within 24 h of incubation, $AFB_1$ was efficiently eliminated by the culture supernatant of C. uredinicola ($84.5{\pm}5.7%$) at $37^{\circ}C$; the elimination was proven to be enzymatic, and the enzyme was thermostable. The biotransformation products of $AFB_1$ detected by HPLC and TLC were proven to be the same compound. Analysis with LCMS showed that $AFB_1$ was bio-transformed to a structurally different compound ($m/z=365[M+Na]^+$), which is first reported. The cytotoxicity study to HeLa cells indicated that culture supernatant-treated $AFB_1$ is less toxic as compared with $AFB_1$.
Reading from transition of Beijing block planning and collective housing in the 1950s
Shao, Shuai East-Asian Society for Urban History 2019 Journal of east-asian urban history Vol.1 No.-
This paper showed that the policy power of the Chinese government in the 1950s was directly linked to the development of housing construction. The author of this study conducted on-site surveys of several collective houses built from 1949 to 1960 that are still standing to photograph and investigate the status of these residential buildings.
Shuai Ji,Yujie Wang,Shikai Gao,Xian Shao,Wei Cui,Yan Du,Mengzhe Guo,Daoquan Tang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
Lots of minor constituents in herbal medicines have shown great potential to devolve into leadcompounds, and their extraction is attracting more and more attention in pharmaceutical industry. Thisstudy provided an example that showed efficient and selective extraction of minor bioactive naturalproducts (prenylatedflavonoids with anti-cancer activities) from an herbal medicine (licorice) using pureionic liquids as green solvents. After investigation of a variety of hydrophobic ionic liquids withultrasonic-assisted extraction, we found that [C8MIM]BF4 (1-octyl-3-methylimidazolium tetrafluor-oborate) was the most effective one for extraction offive representative compounds (isoangustone A,glycycoumarin, licoisoflavanone, licoricidin and glabridin), and solvent to solid ratio, extraction time,extraction temperature and soaking time were then optimized through single-factor experiments andresponse surface methodology. As a result, the established method had significantly higher extractionselectivity than conventional organic solvents, which could be attributed to significant physical change oflicorice microstructures after extraction, as well as strong interactions (especially hydrogen bondinteractions) between [C8MIM]BF4 and prenylatedflavonoids by mechanism study. In addition, 78.92% ofthe extracted prenylatedflavonoids could be readily recovered from [C8MIM]BF4 by using reversed-phasesolid phase extraction, and the recyclability of [C8MIM]BF4 was also confirmed.
Hui Chen,Shuai Shao,Hao Jiang,Guohai Ren,Pengying Du 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
This paper proposes a current sharing method for interleaved LLC resonant converter with secondary partial energy phase shift regulation. An AC voltage source with phase shift regulation is constructed and in series with main transformer at secondary side to balance the output currents. With secondary regulation ability, the current imbalance caused by the resonant tank tolerance can be compensated. The major part of power is delivered to load by main transformer, and small portion power is converted by the auxiliary parts, therefore, the energy loss of the conversion is almost as high as conventional LLC resonant converter. The steady state operating principle of the proposed converter is analyzed, and a prototype with two phases current sharing ability is built-up based on the theoretical analysis.
Zhu, Yu-Lan,Shao, Shuai,Ma, Kui-Rong,Tang, Xue-Ling,Cao, Li,Zhao, Hui-Chao Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.4
Two metal compounds, $[Co(phen)_2(H_2O)_2]{\cdot}2H_2SIP{\cdot}2H_2O$ 1 and $[Ni(phen)_3]{\cdot}2H_2SIP{\cdot}3H_2O$ 2, have been obtained by incorporating 1,10-phenanthroline (phen) and 5-sulfoisophthalic acid monosodium salt ($NaH_2SIP$) ligands under hydrothermal conditions. Meanwhile, the two compounds were characterized by element analysis, IR, XRD, TG-DTA and single-crystal X-ray diffraction. Both 1 and 2 present 3D supramolecular structures via O-H${\cdots}$O hydrogen bond interactions. Luminescent properties for 1 and 2 were also studied. The compound 1 has two fluorescence emission peaks centered at 398 nm attributed to the intraligand emission from the SIP ligand and at 438 nm assigned to the combined interaction of intraligand ${\pi}^*-{\pi}$ transitions of the phen ligand and ligand-to-metal-charge-transfer (LMCT) transitions (${\lambda}_{ex}$ = 233 nm). The compound 2 shows one emission band centered at 423 nm with a shoulder peak at 434 nm which may be originated from the intraligand ${\pi}^*-{\pi}$ transitions of the phen ligand (${\lambda}_{ex}$ = 266 nm).
Yu Zhenjun,Li Yuhan,Shao Shuai,Guo Beichen,Zhang Mengxia,Zheng Lina,Zhang Kun,Zhou Feng,Zhang Li,Chen Chiyi,Jiang Wentao,Hong Wei,Han Tao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Some long noncoding RNAs (lncRNAs), which harbor microRNAs in their gene sequence and are also known as microRNA host gene derived lncRNAs (lnc-MIRHGs), play a dominant role alongside miRNAs, or both perform biological functions synergistically or independently. However, only a small number of lnc-MIRHGs have been identified. Here, multiple liver injury datasets were analyzed to screen and identify the target lncRNA Mir122hg. Mir122hg was mainly enriched in liver tissues with human-mouse homology. In both CCl4-induced acute liver injury and Dgal/LPS-induced fulminant liver failure in mice, Mir122hg was sharply downregulated at the early stage, while a subsequent significant increase was only found in the CCl4 group with liver recovery. Overexpression and silencing assays confirmed that Mir122hg played a protective role in acute injury by promoting hepatocyte proliferation in vivo and in vitro. Consistent with the results of gene enrichment analysis, Mir122hg binding to C/EBPα affected its transcriptional repression, promoted gene transcription of downstream chemokines, Cxcl2, Cxcl3, and Cxcl5, and exerted pro-proliferative effects on hepatocytes through activation of the AKT/GSK-3β/p27 signaling pathway by CXC/CXCR2 complexes. This study identifies a novel lncRNA with protective effects in acute liver injury and demonstrates that the binding of Mir122hg-C/EBPα promotes hepatocyte proliferation via upregulation of CXC chemokine and activation of AKT signaling.
Yu-Lan Zhu,Shuai Shao,Kui-Rong Ma,Xue-Ling Tang,Li Cao,Hui-Chao Zhao 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.4
Two metal compounds, [Co(phen)2(H2O)2]·2H2SIP·2H2O 1 and [Ni(phen)3]·2H2SIP·3H2O 2, have been obtained by incorporating 1,10-phenanthroline (phen) and 5-sulfoisophthalic acid monosodium salt (NaH2SIP) ligands under hydrothermal conditions. Meanwhile, the two compounds were characterized by element analysis, IR, XRD, TG-DTA and single-crystal X-ray diffraction. Both 1 and 2 present 3D supramolecular structures via O–H···O hydrogen bond interactions. Luminescent properties for 1 and 2 were also studied. The compound 1 has two fluorescence emission peaks centered at 398 nm attributed to the intraligand emission from the SIP ligand and at 438 nm assigned to the combined interaction of intraligand π*-π transitions of the phen ligand and ligand-to-metal-charge-transfer (LMCT) transitions (λex = 233 nm). The compound 2 shows one emission band centered at 423 nm with a shoulder peak at 434 nm which may be originated from the intraligand π*-π transitions of the phen ligand (λex = 266 nm).