Two-Step Reset in the Resistance Switching of the Al/TiO_<i>x</i>/Cu Structure

Shao, Xing L.,Zhao, Jin S.,Zhang, Kai L.,Chen, Ran,Sun, Kuo,Chen, Chang J.,Liu, Kai,Zhou, Li W.,Wang, Jian Y.,Ma, Chen M.,Yoon, Kyung J.,Hwang, Cheol S. American Chemical Society 2013 ACS APPLIED MATERIALS & INTERFACES Vol.5 No.21

<P>Two-step reset behaviors in the resistance switching properties of the top Al/TiO<SUB><I>x</I></SUB>/bottom Cu structure were studied. During the electroforming and set steps, two types of conducting filaments composed of Cu and oxygen vacancies (Cu-CF and V<SUB>O</SUB>-CF) were simultaneously (or sequentially) formed when Al was negatively biased. In the subsequent reset step with the opposite bias polarity, the Cu-CFs ruptured first at ∼0.5 V, and formed an intermediate state. The trap-filled V<SUB>O</SUB>-CFs were transformed into a trap-empty state, resulting in a high-resistance state at ∼1 V. Matrix phase in the electrochemical metallization cell can play an active role in resistance switching.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2013/aamick.2013.5.issue-21/am403498q/production/images/medium/am-2013-03498q_0007.gif'></P>

Electronic resistance switching in the Al/TiO(x)/Al structure for forming-free and area-scalable memory.

Shao, Xing Long,Zhou, Li Wei,Yoon, Kyung Jean,Jiang, Hao,Zhao, Jin Shi,Zhang, Kai Liang,Yoo, Sijung,Hwang, Cheol Seong RSC Pub 2015 Nanoscale Vol.7 No.25

<P>Electronic bipolar resistance switching (eBRS) in an Al/TiOx/Al structure, where the TiOx layer was reactively sputter-deposited, was examined in conjunction with a structural analysis using transmission electron microscopy. A thin (3-5 nm) insulating Al(Ti)O-x layer was formed at the bottom Al electrode interface, which provided the necessary asymmetric potential barrier for the eBRS to emerge, whereas the top Al electrode interface appeared to have provided the fluent carrier (electron) injection. The set and reset switching were related to the trapping and detrapping of the carriers at the trap centers, the characteristic energy of which was similar to 0.86 eV, across the entire electrode area. The general features of this material system as the feasible RS memory were insufficient: endurance cycle, <similar to 8000, and retention time at 85 degrees C, 10(6) s. However, the detailed analysis of the switching behavior based on the space-charge limited current conduction mechanism, and its variation with the switching cycles, provided useful information on the general features of the eBRS, which could also be applicable to other binary (or even ternary) metal-oxide RS systems based on the electronic switching mechanism.</P>

Improving Cellulase Production in Trichoderma koningii Through RNA Interference on ace1 Gene Expression

( Wang Shao Wen ),( Miao Xing ),( Gang Liu ),( Shao Wen Yu ),( Juan Wang ),( Sheng Li Tian ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.8

Ribonucleic acid interference (RNAi) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. In this study, the function of the repressor of cellulase expression I (ACEI) was verified in Trichoderma koningii (T. koningii) YC01 through RNAi, and ace1-silenced strains with improved cellulase productivity were obtained. An expression cassette that transcribed the interfering double-stranded RNA (dsRNA) of ace1 was constructed and transformed into T. koningii, and the transformants, in which the expression of ace1 was successfully silenced, were selected. As a result of the ace1 gene silencing, the expression levels of the main cellulase and xylanase genes were elevated, and the enhanced production of total proteins, cellulase, and xylanase was observed in the cultivation. In addition, the downregulation of ace1 resulted in an increasing expression of xyr1, but no clear variation in the expression of cre1, which suggested that ACEI acted as a repressor of the xyr1 transcription, but was not involved in the regulation of the cre1 expression. The results of this work indicate that ace1 is a valid target gene for enhancing enzyme production in T. koningii, and RNAi is an appropriate tool for improving the properties of industrial fungi.

Research on Self-adaptive TFRC Congestion Control of Streaming Media in Wireless Network

Xing Li,Pin Wang,Limin Shao 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.2

Transport layer protocol, for lack of effective congestion control, made an irrational bandwidth utilization ratio between UDP and TCP. Especially for streaming media transport which is easily to cause the network congestion. TFRC (TCP-friendly rate control protocol) congestion control mechanism had solved the problem effectively. However, in the high bit error rate environment of wireless network, TFPC would mistake the bit error for congestion lost packet that degrades throughput excessively. Concerning this issue, this paper put forward a self-adaptive TFRC mechanism which distinguishes congest and bit error effectively by distinguishing parameters, and adjusts the transmission rate dynamically. Simulation result reveals that self-adaptive TFRC mechanism can use link efficiently, reduce delay variation and meet the live transmission requirement of streaming media in wireless network.

Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification

Shao-shuai Yu,Hai-yan Che,Sheng-jie Wang,Cai-li Lin,Ming-xing Lin,Wei-wei Song,Qing-hua Tang,Wei Yan,Wei-quan Qin 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5

Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loopmediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64oC for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.

An improved solid boundary treatment for waveefloat interactions using ISPH method

Xing Zheng,Xipeng Lv,Qingwei Ma,Wenyang Duan,Abbas Khayyer,Songdong Shao 대한조선학회 2018 International Journal of Naval Architecture and Oc Vol.10 No.3

The Smoothed Particle Hydrodynamics (SPH) method has proved to have great potentials in dealing with the wave-structure interactions. Compared with the Weakly Compressible SPH (WCSPH) method, the ISPH approach solves the pressure by using the pressure Poisson equation rather than the equation of state. This could provide a more stable and accurate pressure field that is important in the study of wave-structure interactions. This paper improves the solid boundary treatment of ISPH by using a high accuracy Simplified Finite Difference Interpolation (SFDI) scheme for the 2D wave-structure coupling problems, especially for free-moving structure. The proposed method is referred as the ISPH_BS. The model improvement is demonstrated by the documented benchmark tests and laboratory experiment covering various wavestructure interaction applications.

Defects of Methylation in Blastocyst Induced by Superovulation do not Cause by Abnormal Expression of Dnmts

Xing-Wei Liang,Shao-Chen Sun,Yong-Xun Jin,Seul-Ki Lee,Jung-Woo Kwon,Xiang-Shun Cui,Nam-Hyung Kim 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2

Defects of Methylation in Blastocyst Induced by Superovulation do not Cause by Abnormal Expression of Dnmts

Xing-Wei Liang,Shao-Chen Sun,Yong-Xun Jin,Seul-Ki Lee,Jung-Woo Kwon,Xiang-Shun Cui,Nam-Hyung Kim 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

Superovulation, or ovarian stimulation is a commonly used ART for treatment of human infertility/subfertility. Recent studies suggest that superovulation unaffects methylated imprints acquisition in mouse oocytes during oogenesis, whereas disrupts DNA methylation maintenance in embryos during preimplantation development. However, the mechanisms of defects in methylation maintanence caused by superovulation remain largely unclear. We hypothesized that superovulation may disrupt the expression of DNA methyltransferases (Dnmts), the enzymes which catalyze DNA methylation acquisition and maintenance. The mice were subjected to superovulate with low (6 IU) and high (10 IU) dosage hormone. We examined the global DNA methylation levels in zygotes and DNA methylation of repeated sequences (IAP and Line 1) in blastocyst stage embryos. In addition, we investigated the expression of Dnmts (Dnmt3a, Dnmt3b, Dnmt3l and Dnmt1o) in ovulated oocytes and zygotes. Through staining with antibody 5mC and Di-H3K9 coupled with confocal microscopy, we found that global methylation profiles in zygotes derived from females after low or high dosage hormone treatment were not affected when compared to control counterpart. Moreover, methylation at IAP in blastocysts also was unaffected by superovulation, irrespective of hormone dosage. In contrast, methylation level at Line 1 decreased when the females were administered by high dosage hormone. Furthermore, expression of de novo DNA methyltransferase Dnmt3a, Dnmt3b, Dnmt3L, as well as maintenance Dnmt1o in MII oocytes and zygotes was not disrupted by superovulation. Given superovulation adversely affected methylation maintenance in blastocysts during preimplantation development but with normal expression of Dnmts in oocytes and zygotes, it is indicated that defects of embryonic methylation didn’t originate from abnormal expression of Dnmts.

Identification of Key Genes and Pathways in Peripheral Blood Mononuclear Cells of Type 1 Diabetes Mellitus by Integrated Bioinformatics Analysis

Xing Li,Mingyu Liao,Jiangheng Guan,Zhou Ling,Rufei Shen,Long Min,Shao Jiaqing 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.3

Background: The onset and progression of type 1 diabetes mellitus (T1DM) is closely related to autoimmunity. Effective monitoring of the immune system and developing targeted therapies are frontier fields in T1DM treatment. Currently, the most available tissue that reflects the immune system is peripheral blood mononuclear cells (PBMCs). Thus, the aim of this study was to identify key PBMC biomarkers of T1DM.Methods: Common differentially expressed genes (DEGs) were screened from the Gene Expression Omnibus (GEO) datasets GSE9006, GSE72377, and GSE55098, and PBMC mRNA expression in T1DM patients was compared with that in healthy participants by GEO2R. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein-protein interaction (PPI) network analyses of DEGs were performed using the Cytoscape, DAVID, and STRING databases. The vital hub genes were validated by reverse transcription-polymerase chain reaction using clinical samples. The disease-gene-drug interaction network was built using the Comparative Toxicogenomics Database (CTD) and Drug Gene Interaction Database (DGIdb).Results: We found that various biological functions or pathways related to the immune system and glucose metabolism changed in PBMCs from T1DM patients. In the PPI network, the DEGs of module 1 were significantly enriched in processes including inflammatory and immune responses and in pathways of proteoglycans in cancer. Moreover, we focused on four vital hub genes, namely, chitinase-3-like protein 1 (CHI3L1), C-X-C motif chemokine ligand 1 (CXCL1), matrix metallopeptidase 9 (MMP9), and granzyme B (GZMB), and confirmed them in clinical PBMC samples. Furthermore, the disease-gene-drug interaction network revealed the potential of key genes as reference markers in T1DM.Conclusion: These results provide new insight into T1DM pathogenesis and novel biomarkers that could be widely representative reference indicators or potential therapeutic targets for clinical applications.

An improved solid boundary treatment for wave-float interactions using ISPH method

Zheng, Xing,Lv, Xipeng,Ma, Qingwei,Duan, Wenyang,Khayyer, Abbas,Shao, Songdong The Society of Naval Architects of Korea 2018 International Journal of Naval Architecture and Oc Vol.10 No.3

The Smoothed Particle Hydrodynamics (SPH) method has proved to have great potentials in dealing with the wave-structure interactions. Compared with the Weakly Compressible SPH (WCSPH) method, the ISPH approach solves the pressure by using the pressure Poisson equation rather than the equation of state. This could provide a more stable and accurate pressure field that is important in the study of wave-structure interactions. This paper improves the solid boundary treatment of ISPH by using a high accuracy Simplified Finite Difference Interpolation (SFDI) scheme for the 2D wave-structure coupling problems, especially for free-moving structure. The proposed method is referred as the ISPH_BS. The model improvement is demonstrated by the documented benchmark tests and laboratory experiment covering various wave-structure interaction applications.