The Presence of Open Dentinal Tubules Affects the Biological Properties of Dental Pulp Cells Ex Vivo

Mei-Ying Shao,Zhong-Sen Fu,Ran Cheng,Hui Yang,Li Cheng,Feng-Ming Wang,Tao Hu 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.1

To investigate the effects of open dentinal tubules on the morphological and functional characteristics of dental pulp cells. Morphological changes in human dental pulp cells that were seeded onto dentin discs with open den-tinal tubules were investigated on days 1, 2, 4, and 10 of culture using scanning electron microscopy and fluores-cence microscopy. Samples collected on days 1, 3, 6, 8, and 10 of culture were evaluated for cell proliferation rate and alkaline phosphatase activity. Cultured human dental pulp cells developed a columnar or polygonal morphology and monopolar cytoplasmic processes that extended into the dentinal tubules. The cells formed a multilayer and secreted an extracellular matrix onto the cell surface. Scanning electron microscopy and fluorescence microscopy revealed polarized organization of odontoblasts. Cells seeded onto dentin discs prolifer-ated minimally but showed high levels of ALP activity. Dental pulp cells seeded onto treated dentin discs develop an odontoblast-like phenotype, which may be a potential alternative for use in experimental research on dentinogenesis.

Structure-based design, structure–activity relationship analysis, and antitumor activity of diaryl ether derivatives

Shao-Mei Yang,Fu-Nan Li,Zhi-Ning Huang,Zhong-Shi Zhou,Jin Hou,Man-Yi Zheng,Li-Juan Wang,Yu Jiang,Xin-Yi Zhou,Qiu-Yue Chen,Shan-Hua Li 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

To identify novel therapeutic agents to treatcancer, we synthesized a series of diaryl ether derivatives. Structure–activity relationship studies revealed that thepresence of a chlorine or hydroxyl at the para-position onthe phenyl ring (5h or 5k) significantly enhanced antitumoractivity. Compound 5h had stronger growth inhibitory activityin HepG2, A549, and HT-29 cells than compound 5k,with IC50 values of 2.57, 5.48, and 30.04 lM, respectively. Compound 5h also inhibited the growth of other cells lines,including Hep3B, PLC/PRF5, SMMC-7721, HeLa, andA375, with IC50 values of 2.76, 4.26, 29.66, 18.86, and10.21 lM, respectively. The antitumor activity of compound5h was confirmed by a colony forming assay. Further,our results indicated that the antitumor activity ofcompound 5h may be mediated by enhancing expression ofp21 and cl-caspase3, and leading to apoptosis of cancercells.

A two-stage cascaded foreground seeds generation for parametric min-cuts

( Shao-mei Li ),( Jun-guang Zhu ),( Chao Gao ),( Chun-wei Li ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.11

Parametric min-cuts is an object proposal algorithm, which can be used for accurate image segmentation. In parametric min-cuts, foreground seeds generation plays an important role since the number and quality of foreground seeds have great effect on its efficiency and accuracy. To improve the performance of parametric min-cuts, this paper proposes a new framework for foreground seeds generation. First, to increase the odds of finding objects, saliency detection at multiple scales is used to generate a large set of diverse candidate seeds. Second, to further select good-quality seeds, a two-stage cascaded ranking classifier is used to filter and rank the candidates based on their appearance features. Experimental results show that parametric min-cuts using our seeding strategy can obtain a relative small pool of proposals with high accuracy.

Aristoyunnolin H attenuates extracellular matrix secretion in cardiac fibroblasts by inhibiting calcium influx

Shao-rui Chen,Wen-ping Zhang,Jing-mei Bao,Zhong-bin Cheng,Sheng Yin 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.1

Aristoyunnolin H is a novel aristophyllenesesquiterpenoid isolated from the traditional Chinese medicineAristolochia yunnanensis Franch. The presentresearch was designed to explore the anti-fibrotic effects ofaristoyunnolin H in adult rat cardiac fibroblasts (CFs)stimulated with angiotensin II (Ang II). Western blotanalysis data showed that aristoyunnolin H reduced theupregulation of fibronectin (FN), connective tissue growthfactor and collagen I(Col I) production induced by Ang II inCFs. By studying the dynamic intracellular changes ofCa2?, we further found that while aristoyunnolin H relievedthe calcium influx, it has no effect on intracellular calciumstore release. Meanwhile, aristoyunnolin H also inhibitedthe Ang II-stimulated phosphorylation of Ca2?/calmodulindependentprotein kinase II. In conclusion, aristoyunnolin Hmay attenuate extracellular matrix secretion in vitro byinhibiting Ang II-induced calcium signaling.

Pre-anesthetic clinic internship: new teaching method of pre-anesthesia evaluation for undergraduates

Shao-Hua Zheng,Xiao-Peng Mei 대한치과마취과학회 2021 Journal of Dental Anesthesia and Pain Medicine Vol.21 No.3

Background: This study aimed to observe the effect of internship in a pre-anesthetic clinic on the teaching quality of pre-anesthesia evaluation for undergraduates. Methods: A total of 120 undergraduates from July 2017 to July 2018 in the anesthesia department of our hospital were randomly divided into two groups: pre-anesthetic clinic internship teaching group (n = 60) and traditional teaching group (n = 60). The knowledge in the pre-anesthesia evaluation teaching chapters was evaluated between the two groups of undergraduates. Results: There were no significant differences in the demographic information between the two groups. The scores in the case analysis and theoretical knowledge test in the pre-anesthetic clinic internship teaching group were significantly higher than those in the traditional teaching group. In addition, the students’ satisfaction with the curriculum design was significantly higher in the pre-anesthetic clinic internship teaching group than in the traditional teaching group. Conclusion: Pre-anesthetic clinic internships can improve the quality of pre-anesthesia assessment teaching for undergraduates.

Color correction method for multi-view video based on background segmentation and dominant color extraction

Feng Shao,Gangyi Jiang,Mei Yu,Yo-Sung Ho 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7

Free viewpoint video (FVV) system is one of the most attracting three-dimensional video applications in the future, which can offer depth impression of the observed scenery and allow interactive selection of viewpoint and direction. A color correction method is proposed in this paper to eliminate un-consistent color appearance between viewpoint images. Experimental results show that the proposed method is quite effective.

A Perception-based Color Correction Method for Multi-view Images

( Feng Shao ),( Gangyi Jiang ),( Mei Yu ),( Zongju Peng ) 한국인터넷정보학회 2011 KSII Transactions on Internet and Information Syst Vol.5 No.2

Three-dimensional (3D) video technologies are becoming increasingly popular, as it can provide users with high quality and immersive experiences. However, color inconsistency between the camera views is an urgent problem to be solved in multi-view imaging. In this paper, a perception-based color correction method for multi-view images is proposed. In the proposed method, human visual sensitivity (VS) and visual attention (VA) models are incorporated into the correction process. Firstly, the VS property is used to reduce the computational complexity by removing these visual insensitive regions. Secondly, the VA property is used to improve the perceptual quality of local VA regions by performing VA-dependent color correction. Experimental results show that compared with other color correction methods, the proposed method can greatly promote the perceptual quality of local VA regions greatly and reduce the computational complexity, and obtain higher coding performance.

Survey on the Morbidity of Obesity and Analysis of Related Factors among School Children in Shanghai, China

Cai Mei-Qin,Wang Shao-Mu,Zhang Xiao-Min The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

Objective : To get a better understanding of obesity and etiological factors of obesity among school children. Methods : We have conducted a questionnaire survey of 4542 (2395male and 2147female) age from 7-15 in Shanghai School District to study their 24h nutrition intakes ; we have asked all our survey participating students to take a physical exam and body mass index measurement to determine the morbidity of obesity. Common statistic method was used in analyzing the data. Results : The result shows an over all morbidity (boys and girls) of $12.3\%$ among the students we have studied. Our research shows that boys have a higher rate of morbidity of obesity $17.1\%$ vs. $7.0\%$ for girls. The morbidity for boys starts under age 10, reaches the peak at age 10 ($24.3\%$), then declines after age 11 ; as for girls the morbidity starts at under age 9, reaches the peak at 9, and then declines after 10. Our study also indicates that the daily nutrition intake of protein, fat and carbohydrate by the students have satisfied Chinese Dietary Reference Intakes (DRIs), while the daily intakes of calcium, iron, zinc, and vitamin C are below the $80\%$ of the DRIs suggested value. For students who are obese, our study shows that their average daily intakes of calories and protein have reached $90\%$ of the suggested value by the RNI while the intakes of calcium, iron, and zinc are far below standards. Furthermore, our research shows that the obese students usually intake more calories, proteins and vitamin $B_1$, but less for iron and calcium compared to the normal students. Some other factors such as the education and occupation of the parents, especially the mother, the family history of obesity and the eating habits are important factors that should be closely watched. Conclusion : The morbidity of obesity among boys is higher than girls. Obesity is closely related to the family history, the diet habits and the life styles.

Design, synthesis and biological evaluation of B-region modified diarylalkyl amide analogues as novel TRPV1 antagonists

한영택,Shao-Mei Yang,Xiao-Yuan Wang,Fu-Nan Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.4

Design, synthesis and biological evaluation ofB-region, known to be a dipolar interacting pharmacophore,modified diarylalkyl amide analogues for novelTRPV1 (transient receptor potential channel, vanilloidsubfamily member 1) antagonists was described. A varietyof moieties including guanidines, heterocyclic rings, cinnamides,and a-substituted acetamides were introduced atthe B-region. TRPV1 antagonistic activities of these analogueswere evaluated by 45Ca2? uptake assay in rat DRGneuron. In particular, a,a-difluoroamide 53 exhibited3-fold more potent TRPV1 antagonistic activity (IC50 =0.058 lM) than the parent amide analogue 6.

Asymmetric Coding of Multi-View Video Plus Depth Based 3-D Video for View Rendering

Feng Shao,Gangyi Jiang,Mei Yu,Ken Chen,Yo-Sung Ho IEEE 2012 IEEE transactions on multimedia Vol.14 No.1

<P>The recent years have witnessed three-dimensional (3-D) video technology to become increasingly popular, as it can provide high-quality and immersive experience to end users, where view rendering with depth-image-based rendering (DIBR) technique is employed to generate the virtual views. Distortions in depth map may induce geometry changes in the virtual views, and distortions in texture video may be propagated to the virtual views. Thus, effective compression of both texture videos and depth maps is important for 3-D video system. From the perspective of bit allocation, asymmetric coding of the texture videos and depth maps is an effective way to get the optimal solution of 3-D video compression and view rendering problems. In this paper, a novel asymmetric coding method of multi-view video plus depth (MVD) based 3-D video is proposed on purpose of providing high-quality view rendering. In the proposed method, two models are proposed to characterize view rendering distortion and binocular suppression in 3-D video. Then, an asymmetric coding method of MVD-based 3-D video is proposed by combining two models in encoding framework. Finally, a chrominance reconstruction algorithm is presented to achieve accurate reconstruction. Experimental results show that compared with other methods, the proposed method can obtain higher performance of view rendering under the total bitrate constraint. Moreover, the perceptual visual quality of 3-D video is almost unaffected with the proposed method.</P>