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        • 재유화형 폴리머와 백시멘트의 조성비가 자기수평모르타르의 물리적 특성에 미치는 영향에 관한 실험적 연구

          ( Shao Xu-dong ), 곽규성 ( Kwak Kyu-sung ), 채우병 ( Chae Woo-byung ), 오상근 ( Oh Sang-keun ) 한국구조물진단유지관리공학회 2009 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.13 No.2

          This study investigates the physical properties of the self-leveling mortar according to variable composition set of redispersible polymers and white Portland cement. This materials have to be composed of many types of binders and chemical additives. So it is difficult to decide suitable mixing proportion of composition materials. The self-leveling materials using "0, 2, 4, 6, 8kg" of EVA polymer dispersion ranges are prepared with three types of white Portland cement rations which respectively rangs from "290, 300, 310kg", and were tested for basic characteristics such as flowability, flexural, and compressive strength, bond strength.

        • KCI등재

          Analysis the role of arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis

          Dong-Wei,Di,Lei,Wu,Pan,Luo,Li,Zhang,Tian-Zi,Zhang,Xue,Sun,Shao-Dong,Wei,Chen-Wei,An,Guang-Qin,Guo 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

          The crosstalk between auxin and cytokinin (CK) is important for plant growth and development, although the underlying molecular mechanisms remain unclear. Here, we describe the isolation and characterization of a mutant of Arabidopsis Cytokinin-induced Root Curling 6 (CKRC6), an allele of ANTHRANILATE SYNTHASE ALPHA SUBUNIT 1 (ASA1) that encodes the á-subunit of AS in tryptophan (Trp) biosynthesis. The ckrc6 mutant exhibits root gravitropic defects and insensitivity to both CK and the ethylene precursor 1-aminocyclopropane-1-carboxylicacid (ACC) in primary root growth. These defects can be rescued by exogenous indole-3-acetic acid (IAA) or tryptophan (Trp) supplementation. Furthermore, our results suggest that the ckrc6 mutant has decreased IAA content, differential expression patterns of auxin biosynthesis genes and CK biosynthesis isopentenyl transferase (IPT) genes in comparison to wild type. Collectively, our study shows that auxin controls CK biosynthesis based on that CK sensitivity is altered in most auxin-resistant mutants and that CKs promote auxin biosynthesis but inhibit auxin transport and response. Our results also suggest that CKRC6/ASA1 may be located at an intersection of auxin, CK and ethylene metabolism and/or signaling.

        • KCI등재SCIESCOPUS

          Test on the anchoring components of steel shear keys in precast shear walls

          Shao-Dong,Shen,Peng,Pan,Wen-Feng,Li,Qi-Song,Miao,Run-Hua,Gong 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.24 No.6

          Prefabricated reinforced-concrete shear walls are used extensively in building structures because they are convenient to construct and environmentally sustainable. To make large walls easier to transport, they are divided into smaller segments and then assembled at the construction site using a variety of connection methods. The present paper proposes a precast shear wall assembled using steel shear keys, wherein the shear keys are fixed on the embedded steel plates of adjacent wall segments by combined plug and fillet welding. The anchoring strength of shear keys is known to affect the mechanical properties of the wall segments. Loading tests were therefore performed to observe the behavior of precast shear wall specimens with different anchoring components for shear keys. The specimen with insufficient strength of anchoring components was found to have reduced stiffness and lateral resistance. Conversely, an extremely high anchoring strength led to a short-column effect at the base of the wall segments and low deformation ability. Finally, for practical engineering purposes, a design approach involving the safety coefficient of anchoring components for steel shear keys is suggested.

        • 재유화형 폴리머와 백시멘트의 조성비가 자기수평모르타르의 물리적 특성에 미치는 영향에 관한 실험적 연구

          邵旭東(Shao, Xu-Dong), 곽규성(Kwak, Kyu-Sung), 채우병(Chae, Woo-Byung), 오상근(Oh, Sang-Keun) 한국구조물진단유지관리학회 2009 한국구조물진단학회 학술발표회논문집 Vol.2009 No.2

          This study investigates the physical properties of the self-leveling mortar according to variable composition set of redispersible polymers and white portland cement. This materials have to be composed of many types of binders and chemical additives. So it is difficult to decide suitable mixing proportion of composition materials. The self-leveling materials using "0, 2, 4, 6, 8kg" of EVA polymer dispersion ranges are prepared with three types of white portland cement rations which respectively rangs from "290, 300, 310kg", and were tested for basic characteristics such as flowability, flexural, and compressive strength, bond strength.

        • SCIESCOPUSKCI등재

          Comparison of nitrogen transformation dynamics in non-irradiated and irradiated alfalfa and red clover during ensiling

          Dong,,Zhihao,Li,,Junfeng,Chen,,Lei,Yuan,,Xianjun,Shao,,Tao Asian Australasian Association of Animal Productio 2019 Animal Bioscience Vol.32 No.10

          Objective: To study the contribution of plant enzyme and microbial activities on protein degradation in silage, this study evaluated the nitrogen transformation dynamics during ensiling of non- and irradiated alfalfa (Medicago sativa L.) and red clover (Trifolium pratense L.). Methods: Alfalfa and red clover silages were prepared and equally divided into two groups. One group was exposed to ${\gamma}$-irradiation at a recommended dosage (25 Gky). Therefore, four types of silages were produced: i) non-irradiated alfalfa silage; ii) irradiated alfalfa silage; iii) non-irradiated red clover silage; and iv) irradiated red clover silage. These silages were opened for fermentation quality and nitrogen components analyses after 1, 4, 8, and 30 days, respectively. Results: The ${\gamma}$-irradiation successfully suppressed microbial activity, indicated by high pH and no apparent increases in fermentation end products in irradiated silages. All nitrogen components, except for peptide-N, increased throughout the ensiling process. Proteolysis less occurred in red clover silages compared with alfalfa silages, indicated by smaller (p<0.05) increment in peptide-N and free amino acid N (FAA-N) during early stage of ensiling. The ${\gamma}$-irradiation treatment increased (p<0.05) peptide-N and FAA-N in alfalfa silage at day 1, whereas not in red clover silage; these two nitrogen components were higher (p<0.05) between day 4 and day 30 in non-irradiated silages than the irradiated silages. The ammonia nitrogen and non-protein nitrogen were highest in non-irradiated alfalfa silage and lowest in irradiated red clover silage after ensiling. Conclusion: The result of this study indicate that red clover and alfalfa are two forages varying in their nitrogen transformation patterns, especially during early stages of ensiling. Microbial activity plays a certain role in the proteolysis and seems little affected by the presence of polyphenol oxidase in red clover compared with alfalfaa.

        • KCI등재SCOPUS

          Effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry (Morus alba L.) leaves silage

          Dong,Zhihao,Wang,Siran,Zhao,Jie,Li,Junfeng,Shao,Tao 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.8

          Objective: To explore feed resources capable of replacing regular poor-quality fodder, this study was conducted to evaluate the effects of additives on the fermentation quality, in vitro digestibility and aerobic stability of mulberry leaves silage. Methods: The mulberry leaves were ensiled either untreated (control) or treated with 1×106 cfu/g fresh matter Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), and a combination of 3% molasses and Lactobacillus plantarum (L+M). The fermentation quality and chemical composition were analyzed after 7, 14, 30, and 60 d, respectively. The 60-d silages were subjected to an aerobic stability test and fermented with buffered rumen fluid to measure the digestibility. Results: Inoculating lactic acid bacteria (LAB) resulted in more rapid increase in lactic acid concentrations and decline in pH of mulberry leaves silage as compared control. Higher acetic acid and lower ethanol and ammonia nitrogen concentrations (p<0.05) were observed in the LAB-inoculated silages as opposed to control during ensiling. The LAB-inoculated silages contained lower water-soluble carbohydrates compared with control during the first 14 d of ensiling, and lower neutral detergent fibre (p<0.05) concentrations as compared with non-LAB inoculated silages. Adding molasses alone increased (p<0.05) the digestibility of dry matter (DM). The aerobic stability of mulberry leaves silage was increased by LAB inoculation, whereas decreased by adding glucose or molasses. Conclusion: The LAB inoculation improved fermentation quality and aerobic stability of mulberry leaves silage, while adding glucose or molasses failed to affect the fermentation and impaired the aerobic stability. Inoculating LAB alone is recommended for mulberry leaves especially when ensiled at a relatively high DM.

        • KCI등재SCOPUS

          Evaluating fermentation quality, in vitro digestibility and aerobic stability of a total mixed ration ensiled with different additives on Tibet plateau

          Dong,,Zhihao,Wang,,Siran,Zhao,,Jie,Li,,Junfeng,Liu,,Qinhua,Bao,,Yuhong,Shao,,Tao Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.2

          Objective: To investigate the improvement in utilization efficiency of total mixed ration (TMR) on Tibetan plateau, TMR were ensiled with different additives. Methods: A total of 150 experimental silos were prepared in a completely randomized design to evaluate the six treatments: i) control (without additive), ii) Lactobacillus buchneri (L. buchneri), iii) acetic acid, iv) propionic acid, v) 1,2-propanediol; and vi) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and in vitro analysis, and then subjected to an aerobic stability test for 14 days. Results: Treating with L. buchneri, acetic acid, 1,2-propanediol and 1-propanol decreased propionic acid contents and yeast number, whereas increased (p<0.05) pH, acetic acid and ethanol contents in the fermented TMR. Despite increased dry matter (DM) loss in the TMRs treated with 1,2-propanediol and 1-pronanol, additives did not affect (p>0.05) all in vitro parameters including gas production at 24 h (GP24), GP rate constant, potential GP, in vitro DM digestibility and in vitro neutral detergent fibre digestibility. All additives improved the aerobic stability of ensiled TMR to different extents. Specially, aerobic stability of the ensiled TMR were substantially improved by L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol, indicated by stable pH and lactic acid content during the aerobic stability test. Conclusion: L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol had no adverse effect on in vitro digestibility, while ensiling TMR with the additives produced more acetic acid and ethanol, subsequently resulting in improvement of aerobic stability. There is a potential for some fermentation boosting additives to enhance aerobic stability of fermented TMR on Tibetan plateau.

        • SCIESCOPUSKCI등재

          Effects of calcium propionate on the fermentation quality and aerobic stability of alfalfa silage

          Dong,,Zhihao,Yuan,,Xianjun,Wen,,Aiyou,Desta,,Seare,T.,Shao,,Tao Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9

          Objective: To assess the potency of calcium propionate (CAP) used as silage additive, an experiment was carried out to evaluate the effect of CAP on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages. Methods: Alfalfa was ensiled with four levels of CAP (5, 10, 15, and 20 g/kg of fresh weight [FW]) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test. Results: The increasing proportion of CAP did not affect pH, lactic acid (LA) concentrations and yeast counts, while linearly decreased counts of enterobacteria (p = 0.029), molds (p<0.001) and clostridia (p<0.001), and concentrations of acetic acid (p<0.001), propionic acid (p<0.001), butyric acid (p<0.001), and ethanol (p = 0.007), and quadratically (p = 0.001) increased lactic acid bacteria counts. With increasing the proportion of CAP, the dry matter (DM) loss (p<0.001), free amino acid N (p<0.001), ammonia N (p = 0.004), and non-protein N (p<0.001) contents were linearly reduced, whereas DM (p = 0.048), water soluble carbohydrate (p<0.001) and peptide N (p<0.001) contents were linearly increased. The highest Flieg's point was found in CAP10 (75.9), represented the best fermentation quality. All silages treated with CAP improved aerobic stability as indicated by increased stable hours compared with control. Conclusion: The addition of CAP can suppress the undesirable microorganisms during ensiling and exposure to air, thereby improving the fermentation quality and aerobic stability as well as retarding the proteolysis of alfalfa silage. It is suggested that CAP used as an additive is recommended at a level of 10 g/kg FW.

        • KCI등재

          Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1

          Dong-Wei,Liu,Jia-Hui,Zhang,Feng-Xun,Liu,Xu-Tong,Wang,Shao-Kang,Pan,Deng-Ke,Jiang,Zi-Hao,Zhao,Zhang-Suo,Liu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

          The number of patients with diabetic nephropathy (DN) is still on the rise worldwide, and this requires the development of new therapeutic strategies. Recent reports have highlighted genetic factors in the treatment of DN. Herein, we aimed to study the roles of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and histone 3 lysine 27 trimethylation (H3K27me3) in DN. A model of DN was established by inducing diabetes in mice with streptozotocin. Mouse podocyte clone 5 (MPC5) podocytes and primary podocytes were cultured in normal and high glucose media to observe cell morphology and to quantify PVT1 expression. The roles of PVT1 and enhancer of zeste homolog 2 (EZH2) were validated via loss-of-function and gain-of-function in vitro experiments to identify the interactions among PVT1, EZH2, and forkhead box A1 (FOXA1). The podocyte damage and apoptosis due to PVT1 and FOXA1 were verified with in vivo experiments. PVT1 was highly expressed in MPC5 and primary podocytes in DN patients and in cultures grown in high glucose medium. A large number of CpG (C-phosphate-G) island sites were predicted at the FOXA1 promoter region, where PVT1 recruited EZH2 to promote the recruitment of H3K27me3. The silencing of PVT1 or the overexpression of FOXA1 relieved the damage and inhibited the apoptosis of podocytes in DN, as was evidenced by the upregulated expression of synaptopodin and podocin, higher expression of Bcl-2, and lower expression of Bax and cleaved caspase-3. The key findings of this study collectively indicate that the suppression of lncRNA PVT1 exerts inhibitory effects on podocyte damage and apoptosis via FOXA1 in DN, which is of clinical significance.

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