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Micropropagation and DNA Delivery Studies in Onion Cultivars of Bellary, CO3
Ashwini Malla,Balamurugan Srinivasan,Bala Murugan Shanmugaraj,Sathishkumar Ramalingam 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.1
Onion is one of the most important vegetables grown all over the world. In the present study, rapidly growing and maintainable callus was accomplished using the basal meristem plate and twin scale leaves as explants in Bellary and CO3 varieties for callus induction and in vitro bulb formation. The two cultivars were transformed with Agrobacterium tumefaciens EHA 105 harboring the binary vector pCAMBIA 1301 containing the GUS gene with different concentrations of acetosyringone. The outcome of this study revealed that the frequency of callus induction was maximum on Murashige and Skoog medium (MS) fortified with B5 vitamins supplemented with 0.5 and 1 mg L-1 picloram. Regeneration of plantlets from the callus was observed on MS supplemented with 0.5 mg L-1 each of BAP and KIN and 0.1 mg L-1 NAA. In vitro bulb response was observed on MS with B5 vitamins supplemented with 2.0 mg L-1 BAP. GUS gene integration in the transgenic plants was confirmed by PCR. The maximum GUS gene expression was observed at a concentration of 150 μM acetosyringone in the transformed plants by histochemical assay. This protocol allows effective genetic transformation and regeneration system of onion via in vitro bulb development.
Meenakshi Sundaram Sri Abirami Saraswathi,Dipak Rana,Subbiah Alwarappan,Shanmugaraj Gowrishankar,Prabu Vijayakumar,Alagumalai Nagendran 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-
Poly (ether imide) [PEI] ultrafiltration membranes are coated by polydopamine (PD) and immobilizedwith silver nanoparticles (AgNPs) to improve the permeation, contaminant separation and anti-foulingproperties. The tailored membranes displayed enhanced permeability (97.2 Lm 2 h 1), hydraulicresistance (13.8 kPa/Lm 2 h 1), average roughness (43 nm), contaminant rejection (>97%) with a higherflux recovery ratio (>95%). PEI/PD/Ag membranes showed anti-biofouling property against gramnegative and gram positive bacteria and facilitated the separation of toxic contaminants. The outstandingstability of PD coating and the presence of AgNPs offer effective and safe water separation.