IN-VITRO STUDY OF CO2 EXTRACT OF TERMINALIA CHEBULA IN BREAST CANCER CELL LINE MD-MB-231

Shachi Chandil,Harikishan Bamoriya,D. B. More 셀메드 세포교정의약학회 2021 셀메드 (CellMed) Vol.11 No.3

Cancer is an abnormal growth of cells in body which leads to death. These cells are born due to imbalance in cell proliferation mechanism. In 2018, WHO released new statistics on cancer incidence, mortality, and prevalence worldwide i.e., GLOBOCAN 2018 estimates for 28 types of cancer in which more prevalence of cervix and breast cancer. According to survey, in India about 7.8 million cancer deaths and 11.5 million new cases arise in 2018, which will increase to 19.3 million new cases per year by 2025. Though breast cancer as such is not explained anywhere in Ayurvedic compendia, correlations can be done with the Stana Arbuda. Ayurveda, the ancient system of medicine came into existence 1000's of years ago with an objective of maintaining the health of people and treating diseases. Many herbs used in Ayurveda have been screened for activity against cancer and in-vitro and in-vivo studies have given promising leads. The plant, called as “Mother of Medicine”, Haritaki has been extensively studied for its various ailments because of its extraordinary healing potency. Haritaki (Terminalia chebula Retz.), Family: Combretaceae have a great therapeutic value and is widely distributed in India. Dried fruit of Terminalia chebula contains high quantities phenolic compounds consist of ellagic acid, gallic acid and chebulic acid. The fruit extract of T. chebula is having different biological properties like anticancer, antioxidant, hepatic and renal protective activities etc. In this study, we focus on the use of CO2 extract of Terminalia chebula, on the breast cancer cell line MDA-MB-231. All tests proved that CO2 extract of Terminalia chebula containing active chemical component, therefore our experiment showed the positive results for CO2 extract of Terminalia chebula against breast cancer cell line cancer MDA-MB-231. The MTT assay results were used to evaluate the anti-cancer activity of the extract. The percentage of cell growth and cell viability were calculated from tabulated result values of MTT assay. Cell viability MTT assay also showed significant growth inhibition, at the same time statistical analysis of MTT assay also proved significant results.

Physico-phytochemical Evaluation of Raw Material and CO2 extract of Fruits of Terminalia chebula

Shachi Chandil,Harikishan Bamoriya 셀메드 세포교정의약학회 2022 셀메드 (CellMed) Vol.12 No.1

The present communication attempts to evaluate the physicochemical and preliminary phytochemical studies on the fruit of Terminalia chebula Retz. Combretaceae family. Haritaki is one of the most celebrated herbs in the Indian traditional medicine system, Ayurveda. Terminalia chebula is known to exhibit different properties like anticancer, anti-inflammatory, anti-protozoal, antimicrobial, antioxidant, hepato and renal protective activities, and in the management of metabolic syndrome. As there is no detailed standardisation work reported on fruit, the physicochemical parameters, preliminary phytochemical constants, heavy metals, analysis are carried out. The phytochemical screening indicated the presence Tannin, Alkaloid, Phenol, Carbohydrate, Steroids, Protein and Resin compounds in CO2 extract of Haritaki. The present investigation will helpful in assessing the quality and purity of a crude drug. Thus, the study provides facts that CO2 extract of Haritaki contains medicinally important bioactive phytochemical compounds which justifies the use of plant species as conventional medicine for treatment of many diseases.

Performance Comparison of Watermarking by Sorting and Without Sorting the Hybrid Wavelet Transforms Generated from Sinusoidal and Non-sinusoidal Orthogonal Transforms

H. B. Kekre,Tanuja sarode,Shachi Natu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6

In this paper a watermarking technique using hybrid wavelet transforms obtained from sinusoidal and non-sinusoidal component orthogonal transforms is proposed. Sinusoidal transform DCT and non-sinusoidal transforms Walsh, Haar and Discrete Kekre Transform are used to generate hybrid wavelet transforms namely DCT-Walsh, Walsh-DCT, DCT-Haar, Haar-DCT, DCT-DKT and DKT-DCT. Size of each component transform matrix is varied suitably from 4, 8, 16, 32, and 64 to generate hybrid wavelet transform matrix for host and watermark. The best size combination is further applied column wise and row wise to host and watermark and to embed the watermark middle frequency regions of host is selected. Embedding is first done without sorting the hybrid wavelet transform coefficients of host and watermark and then sorting is applied to observe the difference in the achieved robustness. Performance of proposed technique is evaluated against various attacks to decide whether sinusoidal transform when used as base transform matrix or local transform matrix is more robust.

CLINICAL STUDY ON EFFECT OF VIRECHANA KARMA (THERAPEUTIC PURGATION)

Harikishan Bamoriya,Shachi Chandil,Rani Singh 셀메드 세포교정의약학회 2022 셀메드 (CellMed) Vol.12 No.1

The study has been carried out for the comparative analysis of relevant literature in order to highlight the present topic "Clinical study on effect on Virechana karma (therapeutic purgation)". Total no. of 40 cases has been selected from OPD and IPD of Panchakarma department, through proper history taking and clinical examination prior to Virechana Karma. Only Virechana Karma Yogya subjects were selected for the Virechana Karma. Analysis of Virechan Karma on the basis of Vegiki (purgative bouts), Maniki (quantity), Langiki (clinical features) and Aantiki (end product) features were done in this study. In this study a highly significant relief was found in patients i.e. p<0.001 due to Virechana mainly vitiated Pitta Dosha (the heat energy in the body) and secondary Kapha dosha (mucus) and Vata Dosha (subtle energy associated with movement) are being expelled out which might have accounted for better relief in Virechan Yogya (indicated) individuals (Pitta Pradhan Vyadhi (vitiated pitta), Raktaj Roga (hemopoietic diseases), Shodhan Yogya Avastha (detoxification), etc.) in the above group of patients. Virechana Dravyas have properties like Tikshna (hyperfunction), Sukshma(subtle), Ushna (heat), etc. are described in Ayurvedic classics which play a vital role in the mode of action of Virechana Karma has also been explained under probable mode of action.

Robust Watermarking Technique using Singular Values of Watermark Embedded in Single Mid- row of Column Transformed Host using DCT/DKT-DCT Wavel

Dr. H. B. Kekre,Dr. Tanuja Sarode,Shachi Natu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.3

Type-Specific Incidence and Persistence of HPV Infection among Young Women: A Prospective Study in North India

Datta, Palika,Bhatla, Neerja,Pandey, R.M.,Dar, Lalit,Patro, A. Rajkumar,Vasisht, Shachi,Kriplani, Alka,Singh, Neeta Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3

Background: Infections with human papillomavirus (HPV) are highly prevalent among sexually active young women in India. However, not much is known about the incidence of type-specific human papillomavirus (HPV) infections and their patterns of persistence, especially in the Indian context. Objective: The objective of this study was to evaluate the rate of acquisition and persistence of HPV types in young women. Methods: Women residing in an urban slum in Delhi (n=1300) were followed for 24 months at 6 monthly intervals. Exfoliated cervical cells collected at each visit were tested for the presence of HPV DNA. Genotyping was performed using the reverse line blot assay. Results: The incidence rate for any HPV type was calculated to be 5 per 1000 women-months. Among high risk HPV types, HPV16 had the highest incidence rate followed by HPV59, HPV52 and HPV18, i.e., 3.0, 0.58, 0.41 and 0.35 women per 1000 women-months respectively. The persistence rate was higher for high-risk than low-risk HPV types. Among low-risk types, HPV42, HPV62, HPV84 and HPV89 were found to persist. Whereas almost all high risk types showed persistence, the highest rate was found in women with HPV types 16, 45, 67, 31, 51 and 59. The persistence rate for HPV16 infection was 45 per 1000 women-months. Conclusion: Incident HPV infections and high risk HPV type-specific persistence were found to be high in our study population of young married women. Understanding the patterns of HPV infection may help plan appropriate strategies for prevention programs including vaccination and screening.