Effects of Melatonin on Improvement of Neurological Function in Focal Cerebral Ischemic Rats

Seunghoon,Jinhee Shin,Minkyung Lee,Sang-Kil Lee,Sang-Rae Lee,Kyu-Tae Chang,Yonggeun Hong 한국동물번식학회 2011 Reproductive & developmental biology Vol.35 No.2

Biosynthesis of fraxetin from three different substrates using engineered Escherichia coli

An Seung Hoon,최규식,Ahn Joong-Hoon 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.5

Fraxetin, which is a simple coumarin, is a phytochemical present in medicinal plants, such as Fraxinus rhynchophylla, and Cortex Fraxini. In plants, it serves as a controller of iron homeostasis. The health-enhancing activities of fraxetin, such as anticancer, neuroprotective and antibacterial activities, are known. Scopoletin 8-hydroxylase (S8H) is a key enzyme involved in the synthesis of fraxetin from scopoletin. Scopoletin can be synthesized either from esculetin by O-methylation or from ferulic acid by feruloyl CoA 6′-hydroxylase (F6′H) and 4-coumaric acid CoA ligase (4CL). To enable fraxetin synthesis, the fraxetin biosynthesis pathway was introduced into Escherichia coli. Three distinct routes, from ferulic acid, esculetin, and scopoletin, were designed for the synthesis of fraxetin. In the first approach, E. coli strain harboring S8H was used and found to synthesize 84.8 μM fraxetin from 100 μM scopoletin. Two E. coli strains were used for the other two approaches because these approaches required at least two enzymatic reactions. Through this approach, 41.4 μM fraxetin was synthesized from 100 μM esculetin, while 33.3 μM fraxetin was synthesized from 100 μM ferulic acid.

유형별 청자의 명절 갈등 표현 해석 양상 연구: 취업준비생을 중심으로

한승훈 ( Han¸ Seung-hoon ) 대한영어영문학회 2021 영어영문학연구 Vol.47 No.4

The goal of this study is to investigate language usages of job seekers who participate in conflict family discourses during Korean holiday with respect to hearers’ types. Hearers are classified into three types on the basis of three self-construal types; independent, interdependent, and ambivalent self-consrtual type (Markus & Kitayama, 1991; Ting-Toomey, et al., 2001). Online self-report questionnaire is used to identify demographic data of subjects (N=100), their self-construal types, and linguistic responses to conflict expressions. The focus of analysis is laid on linearly examining a meaning-interpretation and a relation-decision process in order to understand their interpretation patterns through the conversation-developing analysis and the face-negotiation theory. As for conflict expressions, each self-construal type shows unique linguistic results; (a) independent hearers (N=44) predominantly make rebuttals, refusals, or ignorance; (b) interdependent hearers (N=11) make partial refusals, acceptances, or acknowledgments; (c) ambivalent hearers (N=45) make rebuttals, acceptances, or avoidance, especially depending on the given context and their conversational goal. (Sogang University)

중국 생물안전법에 대한 연구 ― 문제점 및 해결방안 중심으로 ―

韩承勋 ( Han¸ Seung-hoon ),李玄雨 ( Lee¸ Hyun-woo ),洪弦廷 ( Hong¸ Hyun-jung ) 한국환경법학회 2020 環境法 硏究 Vol.42 No.1

생명공학 기술은 의료, 위생, 농업, 환경보호, 화공, 식품 및 보건 영역에서 인류의 건강과 생존 환경을 개선하였고, 농림업과 공업의 생산량 및 품질을 향상 시키어 전 세계 경제발전에 많은 공헌을 함과 동시에 생물안전 문제, 윤리 및 도덕과 같은 부정적인 문제들도 가지고 있는 “양날의 검”과 같다. 생물안전은 영어로 안전성을 의미하는 ‘biosafety’ 또는 안보를 의미하는 ‘biosecurity’ 개념으로 이해할 수 있고, 유전자변형생물체의 안전한 관리, 감염성 병원체로 인한 질병의 관리 같은 것은 바이오 안전성(biosafety)으로 이해될 수 있다. 또한, 가축 질병의 만연으로 인한 경제적 손실, 생물테러, 생물무기와 관련될 경우 “생물안보”(biosecurity)라고 부를 수도 있다. 이처럼 생물안전 개념은 국가, 지역, 집단, 개인 혹은 여건·상황에 따라 용어나 내용적 범위를 달리해 쓰이고 있다. 생물안전의 관리주체는 일반적으로 민간, 기업, 지방정부와 중앙정부이며 주요 관리대상은 전염병, 병해충, 유전자변형, 외래종, 기후변화, 오염물질 등이고, 관리범위는 지리적으로 생태계, 지역, 국가, 전 세계이면서 사회, 문화, 환경, 위생 등 여러 분야에서 폭넓게 다루고 있다. 따라서 생물안전에 소홀할 경우 지역사회나 국가를 넘어서 심각할 경우 전 세계까지 확산될 수 있다. 중국은 1990년대부터 생물안전 관련 법률, 법규, 행정명령 형식의 규범성 문건들을 발표하였고 생물경제 발전이 가져온 생물안전 문제에 대한 법률과 법규들을 제정하였다. 그러나 생물안전 선진국가와 달리 중국의 생물안전 관계 법제는 아직 그 수준에 도달하지 못하고 있는 실정이며, 기존 법률과 법규들은 실질적인 생물안전 수요에 비해 부족한 부분들이 있다. 주지하다시피 WHO가 2020년 3월 11일 코로나19(COVID-19) 사태에 대해 팬데믹(Pandemic)을 선언하였으며 전 세계가 심각한 피해를 입었다. 이러한 중대한 생물안전 문제들의 특징은 다양성, 광역성, 집단성, 전파성, 치명성, 복잡성 등을 나타내고 있다. 이런 특징으로 인해 생물안전 문제를 효과적으로 예방하고 해결하기 위해 상당한 수준의 과학적 지식과 경험이 요구되고, 동시에 관련 법체계, 관리체계, 제도들은 가능한 중복을 피하고 간결하게 구성할 필요가 있다. 중국 생물안전 관련 법률과 법규의 주요 문제는 중국의 민법 체계가 단행법 위주의 입법형태를 갖추고 있어 조정 대상 위주로 개별법을 제정하다 보니 관련법률과 법규간 내용상 불일치, 비협조, 모호성을 피할 수 없다. 이것은 생물안전 관리체계에 영향을 주었으며, 특히 중앙과 지방의 복잡한 보고 체계, 지방정부의 응급조치 권한 비효율성, 정보공개의 비신속성, 사법제도 부실 등의 문제 등을 안고 있다. 따라서, 중국은 조속히 생물안전법의 제정을 통해 현행 중국 생물안전 법체계의 구조적 결함을 개선하고, 생물안전에 관계된 법규를 조정할 수 있는 효과적인 법체계를 수립하며, 기존의 복잡하고 중복적인 관리체계를 개선하여 중앙과 지방의 입체적·신속적인 협력을 추진하면서 사법기능을 강화할 것으로 보인다. Biotechnology has improved human health and survival in the areas of medicine, hygiene, agriculture, environmental protection, chemicals, food, health, and contributes to the economic development of the world by improving the production and quality of agriculture and industry. It is like a “double-edged sword” that also has negative issues such as biosafety issues, ethics and morals. In particular, biosafety can be understood as the concept of ‘biosafety’ in English or ‘biosecurity’ in terms of security, and the safety of genetically modified organisms and the management of diseases caused by infectious pathogens. It may also be called “national biosecurity” beyond biosecurity when it comes to economic losses, bioterrorism concerns, and biological weapons due to the prevalence of livestock disease. As such, biosafety concepts are used in different terms or scopes depending on the country, region, group, individual, or situation. Biosafety management agencies are generally private, corporate, local and central government. their main management targets are infectious diseases, pests, genetic modifications, invasive species, climate change and pollutants, and the scope of management is geographically ecosystem, region, country and world. In addition, it covers a wide range of fields such as society, culture, environment and hygiene. Thus, neglect of biosafety can spread beyond communities or countries and, in serious cases, to the whole world. Since the 1990s, China has published normative documents in the form of laws, regulations and executive orders on biosafety, and enacted laws and regulations on biosafety issues brought on by bioeconomic development. However, unlike the advanced biosafety countries, China's biosafety legislation has not yet reached that level, and existing laws and regulations lack the actual biosafety demand. As is well known, WHO declared Pandemic on March 11, 2020 for the Corona 19 (COVID-19), and the world suffered serious damage. These significant biosafety issues are characterized by diversity, wide area, collectiveity, dissemination, fatality, and complexity. Due to these characteristics, a considerable amount of scientific knowledge and experience is required to effectively prevent and solve biosafety problems, and at the same time, related legal systems, management systems, and systems need to be structured as simple as possible, avoiding duplication. The main issue of China's biosafety laws and regulations is that the Chinese civil law system has a legislative form focusing on single law. Individual laws are mainly oriented to mediation, so there are inconsistency, non-cooperation, or ambiguity in the content of related laws and regulations. This has had an impact on the management system in relation to biosafety, especially the central and local complex reporting system, the inefficiency of local governments' first aid measures, the non-existence of information disclosure, and the lack of judicial system. By enacting the Biosafety Act, China will improve the structural deficiencies of the current Chinese biosafety law system, establish an effective legal system to adjust the regulations related to biosafety, and improve the existing complex and redundant management system in the central and local government. It is expected to strengthen the judicial function by promoting the three-dimensional and rapid cooperation.

지속가능한 건설사업관리를 위한 발전방안

이승훈 ( Lee¸ Seung-hoon ) 한국건축시공학회 2021 한국건축시공학회 학술발표대회 논문집 Vol.21 No.2

For the sustainable development of the domestic construction project management field, each CM company should try to diversify in terms of service contract types and scope of work while having internationally competitive technical and service capabilities as follows. First, from the initiation of the service to the completion, the CMr should be able to establish and execute a plan to create specific outcomes. Second, CMr must have the capability to produce weekly or monthly cost, schedule, risk, and scope management reports. In addition, it should be possible to apply the cost plus sliding fee method or to systematically and continuously accumulate and process data generated during the construction process.

Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME)

Seunghoon Song,Seung-Woon Myung 한국분석과학회 2021 분석과학 Vol.34 No.4

Beneficial effects of melatonin on stroke-induced muscle atrophy in focal cerebral ischemic rats

Seunghoon Lee,Jinhee Shin,Yunkyung Hong,Minkyung Lee,Koo Kim,Sang-Rae Lee,Kyu-Tae Chang,Yonggeun Hong 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.1

Muscle atrophy is the result of two opposing conditions that can be found in pathological or diseased muscles: an imbalance in protein synthesis and degradation mechanisms. Thus, we investigated whether exogenous melatonin could regulate muscle components in stroke-induced muscle atrophy in rats. Comparing muscle phenotypes, we found that long-term melatonin administration could influence muscle mass. Muscle atrophy-related genes, including muscle atrophy F-box (MAFbx) and muscle ring finger 1 (MuRF1) were significantly down-regulated in melatonin-administered rats in the gastrocnemius. However, only MAFbx at the mRNA level was attenuated in the soleus of melatonin-administered rats. Insulin-like growth factor-1 receptor (IGF-1R) was significantly over-expressed in melatonin-administered rats in both the gastrocnemius and soleus muscles. Comparing myosin heavy chain (MHC) components, in the gastrocnemius, expression of both slow- and fast-type isoforms were significantly enhanced in melatoninadministered rats. These results suggest that long-term exogenous melatonin administration may have a prophylactic effect on muscle atrophy through the MuRF1/MAFbx signaling pathway, as well as a potential therapeutic effect on muscle atrophy through the IGF-1-mediated hypertrophic signaling pathway in a stroke animal model.

OPTIMIZATION OF WIND TURBINE BLADES FOR REDUCTION OF AIRFOILSELF-NOISE

Seunghoon LEE,Soogab LEE,Jaeha RYI,Jong-SooCHOI 한국신재생에너지학회 2011 AFORE Vol.2011 No.-

Hospital admissions due to endocrine diseases in Korean male firefighters

Seunghoon Ryu,Yong-Jin Lee,Eun-Chul Jang,Soon-Chan Kwon,Kiseok Kim,Yeon-Soon Ahn,Young-Sun Min 대한직업환경의학회 2021 대한직업환경의학회지 Vol.33 No.6

NUMERICAL PREDICTION OF WIND TURBINE AERODYNAMIC NOISE WITH WALL-PRESSURE SPECTRAL MODEL

Seunghoon LEE,Soogab LEE 한국신재생에너지학회 2014 AFORE Vol.2014 No.-