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Seo, Seung-Bo,Kim, Jae-Ho,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1
내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13 세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13을 2% 가용성 전분, 1% 효모 추출물, 1% Na_(2)CO_(3) 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 50℃에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 65℃, pH 8.5-9.0 이었고 80℃이하와 pH 5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용 시켰을 때 49%의 CD 수율을 보였다. To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isolated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1% Na_(2)CO_(3) (pH 8.5) at 50℃ for 24 h and about 80% of maximal activity was also showed in the culture broth of 60℃ for 18 h . Optimum reaction temperature and pH of the partial purified CGTase for soluble starch were 65℃ and pH 8.5-9.0, respectively. The partial purified CGTase were also stable below 80℃ and pH 5.0-10.0. When 1% soluble starch was digested with the partial purified CGTase, the yield of cyclodextrin was 49%.
동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여
박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1
This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.
Syntheses of New Azo Calix[4]arenes Containing Benzo Crown Ether
장승현,정광보 大邱大學校附設 基礎科學硏究所 1998 基礎科學硏究 Vol.15 No.1
New azo calix[4]arenes, 5-benzo-15-crown-5-azo-25,26,27,28-tetrahydroxy calix[4]arene(1), and 5,17-bis(benzo-15-crown-5-azo)25,26,27,28-tetrahydroxy calix[4]arene (2) were prepared by reaction of diazonium salt of 4-aminobenzo-15-crown-5 and clix[4]arene. 4-Aminobenzo-15-crown-5 was synthesized by reduction of 4-nitrobenzo-15-crown-5, obtained from the nitration of monobenzo-15-crown-5.
티타늄 함유 제올라이트 베타 촉매의 합성, 물성조사 및 반응특성 연구
윤보상,안화승 한국화학공학회 2002 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.40 No.1
티타늄 함유 제올라이트 베타(Ti-beta) 촉매를 5가지 수열합성 방법과 알콕사이드 또는 메탈로센을 티타늄 원으로 이용한 2가지 후처리 grafting방법으로 제조하여 제조 방법에 따른 물성 차이를 연구하였다. 모든 촉매의 UV-Vis 스펙트럼에는 제올라이트 격자구조 내에 고립되어 존재하는 티타늄에 해당하는 220㎚의 흡수밴드가 관찰되었으며, grafting 시킨 경우 나노크기의 TiO_2 cluster에 기인하는 240-320㎚의 흡수 밴드가 함께 관찰되었다. 결정 입자는 dry-gel 방법과 seed를 사용한 경우 50㎚이하, 액상 전구체의 수열합성에 의한 기존 방법이나 TiO_2/SiO_2 xcrogel 전구체를 사용한 경우는 200㎚, 불산 분위기 합성에서는 2-5㎛의 평균 입자 크기를 나타내었으며, 분자체 결정에 대한 수율은 Ti-beta(F^-)>Ti-veta(seed)>Ti-Al-beta(xerogel)>Ti-Al-beta(dry-gel)>Ti-Al-beta(conventional)의 순서로 조사되었다. Cyclohexene의 epoxidation 반응은 Ti-Al-beta(conventional), Ti-beta(seed)>Ti-Al-beta(xerogel), Ti-Al-beta(dry-gel)>Ti-beta(F^-)의 순서로 전화율이 나타났으며, epoxide에 대한 선택성은 Ti-Al-beta(xerogel)<Ti-Al-beta(conventional)<Ti-Al-beta(dry-gel)<Ti-beta(seed)<Ti-beta(F^-)의 순서로 증가하였다. Epoxide의 선택성은 알루미늄 함량과 표면의 소수성 정도에 영향을 받음을 확인할 수 있었다. Cyclohexene의 전화율과 epoxide의 선택성은 모든 촉매에서 CH_3CN을 용매로 사용할 때가 CH_3OH보다 높게 나타났다. A critical evaluation of synthesis recipes reported for the large pore zeolite Ti-beta was conducted after a series of titanium containing zeolite beta were prepared by five different hydrothermal synthesis and in two post-synthetic grafting methods using alkoxide or metallocene as a titanium precursor. All Ti-beta prepared by different synthesis routes showed a narrow charge transfer band centered at ca. 220㎚ in UV-Vis spectra, typical of Ti sites isolated in zeolite framework. For those prepared by grafting, the absorption band at 240-320㎚ attributed to nano-sized TiQ clusters were also detected. Particle size of Ti-Al-beta(dry-gel) and Ti-beta(seed) were below 20㎚; Ti-Al-beta(conventional) and Ti-Al-beta(xerogel) below 200㎚; and Ti-beta(F^-) ca. 2-5㎛. Yield of zeolite crystals decreased in the order Ti-beta(F^-)>Ti-veta(seed)>Ti-Al-beta(xerogel)>Ti-Al-beta(dry-gel)>Ti-Al-beta(conventional). For cyclohexene epoxidation, conversions varied in the order Ti-Al-beta(conventional), Ti-beta(seed)>Ti-Al-beta(xerogel), Ti-Al-beta(dry-gel)>Ti-beta(F^-)> with epoxide selectivity in the order Ti-Al-beta(xerogel)<Ti-Al-beta(conventional)<Ti-Al-beta(dry-gel)<Ti-beta(seed)<Ti-beta(F^-). The epoxide selectivity was affected by aluminum content and hydrophobicity of the material. Cyclohexene conversions and epoxide selectivities were higher in aprotic CH_3CN solvent than in CH_3OH.
좌승택,오성보 濟州大學校 産業技術硏究所 1999 산업기술연구소논문집 Vol.10 No.2
School illumination with full volume of light and adequate brightness is designed to help students better focus on their education and produce an optimum atmospnere where students can apply their utmost to learning. This paper present to study actual illumination environment of schools in order to figure out illumination prolems. Furthermore. it attempts to suggest an optimum design for schoolroom illumination through computer simulation to attain qualitative improvement effect in school illumination under the illuminaion plan which is based on illumination maintenance and uniformity ratio that K.S. illumination standard sets.
유승곤,송봉준,이보성 충남대학교 공업교육연구소 1985 論文集 Vol.8 No.1
Water soluble components of Persicaria hydropiper have an insecticidal effect. The extraction mechanism of water solubles from Persicaria hydropiper was studied and it turned out to be a diffusion model. The diffusivity was logarithmically increased with respect to extraction temperature (20…70℃) showing the values in 0.7…2.65×10^-7 cm²/min. Some variables which have an effect on extracted quantity of water solubles were also observed. As a result, optimum operatin conditions and empirical equation were presented for the industrial scale extraction.