Ubiquitin Expression in Soft Coral (Scleronephthya gracillimum) Exposed to Environmental Stresses

Seung Shic Yum 한국유전학회 2006 Genes & Genomics Vol.28 No.2

멧누에 ( Bombyx mandarina ) 난간단백질 유전자의 유전적 분석

성승현(Seung Hyun Sung),김순정(Soon Jeung Kim),염승식(Seung Shic Yum),김종길(Jong Kil Kim),김기세(Ki Sei Kim),김근종(Keun Chong Kim),문준옥(Jun Ok Moon),이병순(Byong Soon Lee),서동상(Dong Sang Suh) 한국유전학회 2001 Genes & Genomics Vol.23 No.4

Two multigene families, high-cysteine A(HcA) and high-cysteine B(HcB), are located within 140 kb region of chorion locus in Bombyx mori. Each family consists of 15 members and one of each members are transcribed in pairs. We report isolation and characterization of HcA and HcB genes of the wild type silkmoth, Bombyx mandarina. In this study, we examined 6 repeating units out of 15 HcA genes and 6 units of 15 HcB genes of B. mori and B. mandarina, respectively, and found that the arrangements and expression units of these are dramatically different. In addition, we found that most of the sequence variations were located within the C-variable region. These results suggest that the likely mechanism of concerted evolution is gene conversion-like event in HcA/HcB gene pairs.

Nucleotide Sequence of ${\beta}-tubulin$ Gene from the Soft Coral Scleronephthya gracillimum $(K\ddot{u}kenthal)$

Yum, Seung-Shic,Woo, Seon-Ock,Chang, Man,Lee, Taek-Kyun,Song, Jun-Im The Korean Society of Oceanography 2005 Ocean science journal Vol.40 No.1

We cloned the complete cDNA of the ${\beta}-bubulin$ from the soft coral, Scleronephthya gracillimum $(K\ddot{u}kenthal)$ (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5'-rapid amplification of cDNA end (RACE) technique. The full-length cDNA of the S. gracillimum ${\beta}-tubulin$ comprised 1541 bp, not including the poly $A^+$ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues 16402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that the sequence we have obtained is, indeed, the full-length cDNA of the ${\beta}-bubulin$ gene in S. gracillimum.

Screening of Ecotoxicant Responsive Genes and Expression Analysis of Benzo[a]pyrene-exposed Rockfish (Sebastes schlgeli)

Yum, Seung-Shic,Woo, Seon-Ock,Lee, Taek-Kyun The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.2

Benzo[a]pyrene is a representative ecotoxicant in marine environment and a model compound of polycyclic aromatic hydrocarbons, which has an ability to bioaccumulate in aquatic organisms. This study aimed to identify molecular biomarkers suitable for assessing environmental pollution using a microarray technique. We examined the effects of benzo[a]pyrene on gene expressions in the rockfish, Sebastes schlegeli. We constructed the subtractive cDNA library with hepatic RNA from benzo[a]pyrene-exposed and non-exposed control fish. From the library 10,000 candidate clones were selected randomly and cDNA microarray was constructed. We determined benzo[a]pyrene-responsive genes using a high-density microarray. Statistical analysis showed that approximately 400 genes are significantly induced or reduced by benzo[a]pyrene treatment ($2\;{\mu}m$). Especially gene expression changes of 4 candidate clones among the up- or down-regulated genes were investigated in 6, 12 and 24 hr BaP-exposed fish groups. Many methods have been developed to monitor marine environmental status, which depend on quantifying the levels of the toxic components in polluted seawater or on ecological accessing, such as species diversity or richness. However, those methods could not provide information on physiological or genetic changes induced by such environmental stresses. Comparing with the conventional methods, these data will propose that benzo[a]pyrene-responsive genes can be useful for biological risk assessment of polycyclic aromatic hydrocarbons on marine organism at molecular level.

Characterization of the stress-inducible OsNCED3 promoter in different transgenic rice organs and over three homozygous generations.

Bang, Seung Woon,Park, Su-Hyun,Jeong, Jin Seo,Kim, Youn Shic,Jung, Harin,Ha, Sun-Hwa,Kim, Ju-Kon Springer-Verlag [etc.] 2013 Planta Vol.237 No.1

<P>To be effective in crop biotechnology applications, gene promoters need to be stably active over sequential generations in a population of single-copy transgenic lines. Most of the stress-inducible promoters characterized in plants thus far have been analyzed at early (T?, T1 or T2) generations and/or by testing only a small number of transgenic lines. In our current study, we report our analysis of OsNCED3, a stress-inducible rice promoter involved in ABA biosynthesis, in various organs and tissues of transgenic rice plants over the T(2-4) homozygous generations. The transgene copy numbers in the lines harboring the OsNCED3:gfp construct were determined and six single- and two double-copy transgenic lines were analyzed for promoter activity in comparison with the Wsi18, a stress-inducible promoter previously characterized. The exogenous promoter activities were found to be significantly enhanced in the roots and leaves, whereas zero or low levels of activity were evident in grains and flowers, under drought and high-salinity conditions. The highest induction levels of gfp transcripts in the OsNCED3:gfp plants upon drought treatments were 161- and 93-fold in leaves and roots, respectively, and these levels were comparable with those of gfp transcripts in the Wsi18:gfp plants. A comparison of the promoter activities between the T2-T4 plants revealed that comparable activity levels were maintained over these three homozygous generations with no evidence of silencing. Thus, our results provide the OsNCED3 promoter that is stress-inducible in a whole rice plant except for in the aleurones and endosperm and stably active over three generations.</P>

The activities of four constitutively expressed promoters in single-copy transgenic rice plants for two homozygous generations.

Bang, Seung Woon,Park, Su-Hyun,Kim, Youn Shic,Choi, Yang Do,Kim, Ju-Kon Springer-Verlag [etc.] 2015 Planta Vol.241 No.6

<P>We have characterized four novel constitutive promoters ARP1, H3F3, HSP and H2BF3 that are active in all tissues/stages of transgenic plants and stable over two homozygous generations. Gene promoters that are active and stable over several generations in transgenic plants are valuable tools for plant research and biotechnology. In this study, we characterized four putative constitutive promoters (ARP1, H3F3, HSP and H2BF3) in transgenic rice plants. Promoter regions were fused to the green fluorescence protein (GFP) reporter gene and transformed into rice. Single-copy transgenic lines were then selected and promoter activity was analyzed in various organs and tissues of two successive homozygous generations. All four promoters showed a broad expression profile in most tissues and developmental stages, and indeed the expression of the ARP1 and H3F3 promoters was even greater than that of the PGD1 promoter, a previously described constitutive promoter that has been used in transgenic rice. This observation was based on expression levels in leaves, roots, dry seeds and flowers in both the T2 and T3 generations. Each promoter exhibited comparable levels of activity over two homozygous generations with no sign of transgene silencing, which is an important characteristic of promoters to be used in crop biotechnology applications. These promoters therefore have considerable potential value for the stable and constitutive expression of transgenes in monocotyledonous crops.</P>

Overexpression of <i>OsTF1L,</i> a rice HD‐Zip transcription factor, promotes lignin biosynthesis and stomatal closure that improves drought tolerance

Bang, Seung Woon,Lee, Dong‐,Keun,Jung, Harin,Chung, Pil Joong,Kim, Youn Shic,Choi, Yang Do,Suh, Joo‐,Won,Kim, Ju‐,Kon BLACKWELL 2019 PLANT BIOTECHNOLOGY JOURNAL Vol.17 No.1

<P><B>Summary</B></P><P>Drought stress seriously impacts on plant development and productivity. Improvement of drought tolerance without yield penalty is a great challenge in crop biotechnology. Here, we report that the rice (<I>Oryza sativa</I>) homeodomain‐leucine zipper transcription factor gene, <I>OsTF1L</I> (<I>Oryza sativa transcription factor 1‐like</I>), is a key regulator of drought tolerance mechanisms. Overexpression of the <I>OsTF1L</I> in rice significantly increased drought tolerance at the vegetative stages of growth and promoted both effective photosynthesis and a reduction in the water loss rate under drought conditions. Importantly, the <I>OsTF1L</I> overexpressing plants showed a higher drought tolerance at the reproductive stage of growth with a higher grain yield than nontransgenic controls under field‐drought conditions. Genomewide analysis of <I>OsTF1L</I> overexpression plants revealed up‐regulation of drought‐inducible, stomatal movement and lignin biosynthetic genes. Overexpression of <I>OsTF1L</I> promoted accumulation of lignin in shoots, whereas the RNAi lines showed opposite patterns of lignin accumulation. <I>OsTF1L</I> is mainly expressed in outer cell layers including the epidermis, and the vasculature of the shoots, which coincides with areas of lignification. In addition, <I>OsTF1L</I> overexpression enhances stomatal closure under drought conditions resulted in drought tolerance. More importantly, OsTF1L directly bound to the promoters of lignin biosynthesis and drought‐related genes involving <I>poxN/PRX38</I>,<I> Nodulin protein</I>,<I>DHHC4</I>,<I>CASPL5B1</I> and <I>AAA‐type ATPase</I>. Collectively, our results provide a new insight into the role of <I>OsTF1L</I> in enhancing drought tolerance through lignin biosynthesis and stomatal closure in rice.</P>

다해상도를 이용한 DCT 기반의 이미지 워터마킹

신윤식(Youn Shic Shin),김용욱(Yong Wook Kim),오승룡(Oh Seung Ryong),허도근(Do Geun Huh) 한국통신학회 2002 한국통신학회 학술대회논문집 Vol.2002 No.7

Screening of Differentially Expressed Genes in Diesel Oil-exposed Marine Fish Using DD-PCR

Woo, Seon-Ock,Yum, Seung-Shic,Yim, Un-Hyuk,Lee, Yaek-Kyun The Korean Society of Toxicogenomics and Toxicopro 2006 Molecular & cellular toxicology Vol.2 No.4

The exploration of genes which expressions are changed by exposure to ecotoxicants or pollutants can provide the important information about the reaction mechanisms in the body as well as adaptation to exterior stimulus or environmental changes. Also they can be developed as biomarkers for the detection of environmental pollution. Differential display polymerase chain reaction (DD-PCR) technique has been usefully used to hunt the clones which expressions are up-regulated or down-regulated by exterior changes and this study aimed to search for those clones in diesel oil-exposed rockfish (Sebastes schlegeli) using DD-PCR. The RNA isolated from liver of 20 ppb diesel oil-exposed rockfish was used for screening of the differentially displayed genes and total 44 differentially expressed genes (DEG) are detected then their nucleotide sequences were analyzed. The present data provided the general information about the effect of diesel oil contamination on marine organism and further more the primary step in development of new biomarkers for marine environmental pollution or ecotoxicological stresses.

Analysis of the APX, PGD1 and R1G1B constitutive gene promoters in various organs over three homozygous generations of transgenic rice plants.

Park, Su-Hyun,Bang, Seung Woon,Jeong, Jin Seo,Jung, Harin,Redillas, Mark Christian Felipe Reveche,Kim, Hyung Il,Lee, Kang Hyun,Kim, Youn Shic,Kim, Ju-Kon Springer-Verlag [etc.] 2012 Planta Vol.235 No.6

<P>We have previously characterized the constitutively active promoters of the APX, PGD1 and R1G1B genes in rice (Park et al. 2010 in J Exp Bot 61:2459-2467). To have potential crop biotechnology applications, gene promoters must be stably active over many generations. In our current study, we report our further detailed analysis of the APX, PGD1 and R1G1B gene promoters in various organs and tissues of transgenic rice plants for three (T?????????) homozygous generations. The copy numbers in 37 transgenic lines that harbor promoter:gfp constructs were determined and promoter activities were measured by real-time qPCR. Analysis of the 37 lines revealed that 15 contained a single copy of one of the three promoter:gfp chimeric constructs. The promoter activity levels were generally higher in multi-copy lines, whereas variations in these levels over the T????????? generations studied were observed to be smaller in single-copy than in multi-copy lines. The three promoters were further found to be highly active in the whole plant body at both the vegetative and reproductive stages of plant growth, with the exception of the APX in the ovary and R1G1B in the pistil and filaments where zero or very low levels of activity were detected. Of note, the spatial activities of the PGD1 promoter were found to be strikingly similar to those of the ZmUbi1, a widely used constitutive promoter. Our comparison of promoter activities between T???, T??? and T??? plants revealed that the APX, PGD1 and R1G1B promoters maintained their activities at comparable levels in leaves and roots over three homozygous generations and are therefore potentially viable alternative promoters for crop biotechnology applications.</P>